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Topic:Chromatography
Chromatography is an analytical technique used to separate, identify, and quantify components within a mixture. In equine research, chromatography is applied to study various biological samples, such as blood, urine, and tissue, to investigate the metabolic and physiological processes in horses. This technique is instrumental in detecting and analyzing compounds like hormones, drugs, and nutrients, providing insights into equine health and performance. Different types of chromatography, including gas chromatography (GC) and high-performance liquid chromatography (HPLC), are utilized to achieve precise and reliable results. This page compiles peer-reviewed research studies and scholarly articles that explore the applications, methodologies, and findings of chromatography in equine science.
Modification of present procedures for the determination of conjugated estrogens (equine) in various low-dosage forms. The presently used procedures for the colorimetric determination of conjugated estrogens (equine) have been modified for the assay of tablets, capsules, liquids, and creams of low declaration per dosage unit. Modifications in sample chromatographic column preparation and elution have provided a more efficient extraction of the steroids.
Composition of neutral lipids from erythrocytes of common mammals. The neutral lipids of the erythrocytes were investigated in several common mammals: cow, dog, goat, horse, pig, rabbit, rat, and sheep. Cholesterol content was determined by gas-liquid, thin-layer, and column chromatography, the last in conjunction with the IR spectrophotometry. The three methods yielded similar results. In every species investigated, cholesterol was the major neutral lipid; cholesteryl esters, triglycerides, and free fatty acids were detected only in trace amounts. It is concluded that these substances may have been contaminants from plasma lipoproteins or leukocytes rather t...
