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Topic:Diagnostic Technique
Diagnostic techniques in equine medicine encompass a range of procedures and tools used to identify diseases, injuries, or other health conditions in horses. These techniques include imaging methods such as radiography, ultrasonography, and magnetic resonance imaging (MRI), as well as laboratory tests like blood work and tissue biopsies. Each diagnostic method provides specific information that can aid in the assessment and management of equine health issues. Radiography is commonly used for evaluating bone structures, while ultrasonography is useful for soft tissue examination. MRI offers detailed images of both soft and hard tissues, though its use is limited by cost and availability. Laboratory tests can detect biochemical and hematological changes indicative of disease. This page compiles peer-reviewed research studies and scholarly articles that explore the development, application, and efficacy of various diagnostic techniques in equine veterinary practice.
[Surgical treatment of an obliterating thrombosis of the jugular vein in a horse. Use of synthetic vessel prosthesis]. A 6-year-old trotter gelding presented with exercise intolerance and swelling of the left side of the head during exercise and grazing. The complaints were caused by a complete thrombosis of the left jugular vein. In this case report a surgical approach is described in which a bypass was created with a synthetic vascular graft. After treatment the horse was capable of racing at his former level. The patency of the synthetic graft was 11 months.
Use of laparoscopic equipment to divide abdominal adhesions in a filly. Exploratory laparoscopy of the right dorsal portion of the abdominal cavity was performed on a Standardbred filly because of signs of mild abdominal pain of 7 days' duration. On the basis of clinical examination, diagnosis was suppurative peritonitis, abdominal adhesions in the area of the right ovary, and right displacement and impaction of the pelvic flexure of the ascending colon. During laparoscopy, an abdominal adhesion between the right uterine horn, the cecum, and the pelvic flexure was identified. The abdominal adhesions were either stretched with laparoscopic forceps used as a probe o...
Ocular lesions in horses with lymphosarcoma: 21 cases (1977-1997). To determine the most common ocular lesions in horses with lymphosarcoma. Methods: Retrospective study. Methods: 79 horses histologically confirmed to have lymphosarcoma. Methods: Ophthalmic examinations were performed by a single individual. Results: 21 of 79 horses had lesions involving the eye or ocular adnexa. Infiltration of the palpebral conjunctiva and eyelids was the most common lesion (n = 11). Other lesions included uveitis (n = 4), corneoscleral masses (2), third eyelid masses (2), and diffuse retrobulbar infiltrates (2). Conclusions: In horses with lymphosarcoma, ocular lesions may...
[Determination of fibrinogen levels in the horse with the heat-precipitation methods of Schalm and Millar]. The purpose of this study was to investigate the usefulness of two heat-precipitation techniques (Schalm- and Millar-method) as screening tests to measure plasma fibrinogen concentration in horses. Based on the measurement of samples from 108 different horses, the coefficient of correlation (CC) for the relationship between the results with the Schalm- and with the reference-method (Jacobsson) were much lower (r = 0.78) than between the Millar- and Jacobsson-method (r = 0.94). Furthermore the Schalm-method was less precise as reflected by the greater coefficient of variation (CV, within-run pr...
Tenoscopic anatomy of the equine carpal flexor synovial sheath. To describe the tenoscopic anatomy of the carpal sheath of the flexor tendons (carpal sheath) viewed from a lateral approach. Methods: Tenoscopic observation of structures within the carpal sheath subsequently confirmed by dissection. Methods: 12 equine cadaveric forelimbs. Methods: The limbs were positioned lateral side up with the carpus slightly flexed. After distention of the carpal sheath, a portal for the arthroscope was made approximately 3 cm proximal to the distal radial physis and 2.5 cm caudal to the radius between the tendons of the ulnaris lateralis and lateral digital extensor mu...
Effect of alpha 2-adrenergic, cholinergic, and nonsteroidal anti-inflammatory drugs on myoelectric activity of ileum, cecum, and right ventral colon and on cecal emptying of radiolabeled markers in clinically normal ponies. To determine effect of xylazine hydrochloride (XYL), yohimbine hydrochloride (YOH), bethanechol chloride (BET), neostigmine methyl sulfate (NEO), or flunixin meglumine (FLU) on ileocecocolic myoelectric activity and passage of radiolabeled markers from the cecum. Methods: 6 healthy adult ponies. Methods: A cecal cannula was surgically implanted, and 12 were sutured to the ileum, cecum, and right ventral colon. After a 12-hour nonfeeding period, 370 MBq of technetium 99m-labeled sulfur colloid in egg albumen and 37 MBq of indium 111-labeled diethyltriaminepentaacetic acid in 60 ml of water were...
Effect of erythromycin lactobionate on myoelectric activity of ileum, cecum, and right ventral colon, and cecal emptying of radiolabeled markers in clinically normal ponies. To determine the effect of erythromycin lactobionate (ERY) on ileocecocolic myoelectric activity and passage of radiolabeled markers from the cecum. Methods: 6 healthy adult ponies. Methods: After a 12-hour nonfeeding period, 370 MBq of technetium 99m-labeled sulfur colloid in egg albumen and 37 MBq of indium 111-labeled diethyltriaminepentaacetic acid in 60 ml of water were administered directly into the cecal apex. The following drug concentrations were tested: ERY, 0.01, 0.10, 1.0, and 10.0 mg/kg of body weight; ERY, 0.10 mg/kg bolus; and saline (0.9% NaCl) solution, 10 ml. All treatments, ...
Antipyrine pharmacokinetics and urinary excretion in female horses. To measure renal clearance of antipyrine and urinary excretion of antipyrine (AP) metabolites in horses by use of validated high-performance liquid chromatography (HPLC) methods. Methods: 8 Standardbred mares. Methods: HPLC methods for measurement of AP in equine plasma and AP and its metabolites in equine urine were validated. Antipyrine (20 mg/kg of body weight) was administered i.v., and blood samples and urine specimens were collected over 24 hours. Results: Median plasma clearance of AP in horses was 6.2 ml/min/kg, of which < 2% could be attributed to renal clearance. Urinary excretion...
Myoelectric activity of the ileum, cecum, and right ventral colon, and cecal emptying of radiolabeled markers in clinically normal ponies. To determine normal cecal emptying curves for liquid- and solid-phase radiolabeled markers and to further define myoelectric patterns of the ileum, cecum, and colon in healthy ponies. Methods: 6 adult ponies. Methods: A cecal cannula and 12 bipolar Ag-AgCl recording electrodes were sutured to the ileum, cecum, and right ventral colon of the ponies. Radioisotopes, indium 111-labeled diethyltriaminepentaacetic acid (111In-DTPA) and technetium 99m (99mTc)-labeled sulfur colloid bound to egg albumen, were introduced through the cannula directly into the cecal body. Movement of these markers from t...
[Radiotherapy in veterinary medicine (review)]. A review of the latest literature concerning the present level of radiation therapy in veterinary medicine is given. In a general section physico-technical as well as biological fundamentals are discussed. In the special part of the paper indications for a radiation therapy of dogs, cats and horses are stated. In this respect the basis for a decision is the TNM-classification into different clinical stages according to the directions of the WHO. Tumors of the hemolymphatic system are very responsive to radiation therapy. While epithelial tumors are sensitive, tumors arising from the mesenchyma...
A microtiter plate assay for the determination of uronic acids. The amount of uronic acid residues in samples containing glycosaminoglycans or pectin is an important parameter in the quantitative and structural analysis of these complex carbohydrates. This paper describes a method to determine the content of uronic acids in biological samples, using conventional polystyrene microtiter plates and microtiter plate-reading equipment with standard interference filters (i.e., 540 or 492 nm). This assay is a modification of a commonly used procedure, viz. hydrolysis of uronic acid containing carbohydrate polymers in 80% sulfuric acid containing tetraborate ions ...
Chorionic gonadotropin secretion is associated with an inhibition of follicular growth and an improvement in oocyte competence for in vitro maturation in the mare. This study reports the follicular growth and oocyte competence for in vitro maturation and fertilization under the influence of circulating eCG. Three to 7 successive ultrasound-guided follicular punctures were performed on 4 pregnant mares from Day 23 until Day 75 of pregnancy and on 5 control mares whose embryonic vesicle was crushed on Day 22. All follicles larger than 5 mm were punctured 24 h after the largest follicle reached 18 mm. Expanded cumulus oocyte complexes (COCs) were stained at recovery to analyze the nuclear stage. Compact COCs were cultured in vitro for 46 h and either staine...
Association between bronchoalveolar lavage cytologic features and airway reactivity in horses with a history of exercise intolerance. To correlate indices of airway reactivity to bronchoalveolar lavage (BAL) fluid cytologic features in horses with a recent decline in exercise tolerance. Methods: 20 actively working horses from 2 to 24 years old. Methods: Bronchoalveolar lavage fluid samples were obtained and analyzed. Forced oscillatory mechanics (1-7 Hz) technique was used for measurements of total respiratory system resistance (RRS), compliance (CRS), and resonant frequency (fres). Changes in RRS (1 Hz) during histamine challenge were used to generate histamine dose-response curves, from which the provocative concentration...
Reliability of coprological diagnosis of Anoplocephala perfoliata infection. Three coprological methods were tested to establish the reliability of in vivo diagnosis of Anoplocephala perfoliata. A total of 107 faecal samples were analyzed, and the presence of tapeworms were confirmed postmortem in 24 animals with burdens that ranged from 1 to 248 worms; most of them (71%) with less than 100 parasites. Best results were obtained with a combination of two sedimentation/flotation methods, detecting only half the parasitized animals (54% sensitivity). No relationship could be established between tapeworm burden and egg detection, but results indicate that coprological meth...
Evaluation of digital and laminar blood flow in horses given a low dose of endotoxin. To measure blood flow in the palmar digital artery and laminae corium, using ultrasonic and laser Doppler flowmetry, respectively. Methods: 6 healthy horses. Methods: Digital blood flow and laminar perfusion, respectively, were measured by placing a flow probe around the palmar digital artery and a laser Doppler flow probe in a hole in the dorsal aspect of the hoof wall. All horses were given saline (0.9% NaCl) solution (1 L, IV, during a 30-minute period). Seven days later, each horse was given endotoxin (0.1 microgram/kg of body weight, IV, in 1 L of saline solution, during a 30-minute perio...
Effect of pin hole size and number on in vitro bone strength in the equine radius loaded in torsion. To determine the effect of pin hole size and number on the breaking strength of the adult equine radius when loaded in torsion to failure. Methods: 54 pairs of equine radii from adult horses. Methods: For test one, 12 pairs of radii were used to determine the effect of pin hole size on torsional breaking strength. A 6.35-mm hole was drilled in 1 radius, and a 9.5-mm hole was drilled in the contralateral radius. For test two, 36 pairs of radii were randomly assigned to 1 of 3 treatment groups (n = 12) to determine the effect of pin hole number on the torsional breaking strength of the equine ra...
Screening for bovine papillomavirus in peripheral blood cells of donkeys with and without sarcoids. Papillomaviral DNA has been identified in peripheral blood cells of both cattle and humans with and without associated disease and it has been suggested that such cells may act as sites of viral latency. In order to investigate the possibility of latent papillomaviral infection in the aetiopathogenesis of the equine sarcoid, peripheral blood derived DNA samples from 20 healthy and 34 sarcoid-affected donkeys were subject to polymerase chain reaction (PCR) using papillomaviral specific primers. Analysis of blood derived DNA samples failed to demonstrate the presence of papillomaviral DNA in any...
Videoendoscopic assessment of dynamic laryngeal function during exercise in horses with grade-III left laryngeal hemiparesis at rest: 26 cases (1992-1995). To evaluate laryngeal function by means of videoendoscopy during high-speed treadmill exercise in racehorses with grade-III left laryngeal hemiparesis at rest and to determine outcome of treatment. Methods: Retrospective study. Methods: 26 racehorses. Methods: Videoendoscopy of the larynx was performed while horses were at rest and exercising on a treadmill. Horses were classified as having grade-IIIA, -IIIB, or -IIIC laryngeal hemiparesis on the basis of the degree of arytenoid cartilage abduction maintained during exercise. Postoperative racing performance was determined by evaluating race r...
Uptake of drugs from topically applied anti-inflammatory preparations applied to racing animals. To determine whether a drug detected in the blood or urine of a racing animal could have penetrated through the skin from a topically applied preparation. Methods: Blood and urine of dogs and horses were analysed after topical administration of three common nonsteroidal anti-inflammatory preparations. Methods: Dimethylsulphoxide was analysed using a gas chromatograph with a flame photometric detector. Phenylbutazone, its metabolites and lignocaine were analysed using a gas chromatograph with a mass selective detector. Results: Dimethylsulphoxide, phenylbutazone and lignocaine were detected in ...
Strains of Actinobacillus spp. from diseases of animals and ostriches in Zimbabwe. Among the Actinobacillus spp. only A. lignieresii represents a homogenous and well studied taxon. However, haemolytic and non-haemolytic strains of A. equuli and A. suis are also isolated from a wide range of diseases in a variety of hosts. These isolates often pose problems in definitive identification. Consequently, several studies have been published, emphasizing the need for detailed studies to reclassify various members of this genus and also to assess their disease significance. We isolated 48 strains of Actinobacillus from clinical cases in horses, cattle, sheep, cat, pigs and ostrich. ...
Assessing the fertility potential of equine semen samples using the reducible dyes methylene green and resazurin. The objective of this study was to determine if spermatozoal reduction of the dyes methylene green to colorless and resazurin to pink or colorless was associated with the fertility potential of an equine semen sample. Fifty samples from 38 stallions were evaluated for the number of spermatozoa per milliliter and number of motile sperm per milliliter. Methylene green (20 micrograms/mL of semen) or resazurin (85 micrograms/mL of semen) was added to 3-mL aliquots of semen. Semen samples were identified as having low fertility potential (< 200 x 10(6) total cells/mL and or = 200 x 10(6) total ...
Fourier-transform infrared spectroscopic studies on the coordination of the side-chain COO- groups to Ca2+ in equine lysozyme. Interactions between Ca2+ and the Asp side chains in the Ca2+-binding site of equine lysozyme were investigated by Fourier-transform infrared (FT-IR) spectroscopy. In the spectrum of equine lysozyme, the intensities of the bands at about 1595 cm-1 and 1578 cm-1 in the region of the COO antisymmetric stretches increased upon Ca2+ binding. In the region of the COO- symmetric stretches, the loss of intensity at about 1388 cm-1 and gains of intensities at about 1423 cm-1 and 1403 cm-1 were observed due to Ca2+ binding to equine lysozyme. The spectral changes for equine lysozyme indicate that the C...
Genetic variation of envelope gp90 gene of equine infectious anemia virus isolated from an experimentally infected horse. Six strains of equine infectious anemia virus (EIAV) were recovered from febrile and non-febrile stages of a horse experimentally infected with the P337-V70 strain given once to a horse. The env gp90 genes of the isolates, the P337-V70 and P337-V26, avirulent virus derived from the P337-V70 strain, were sequenced. A comparison of the gp90 gene sequences revealed that amino acid variations among the viruses tested showed as high as 8.2 to 11.5%. In addition, the comparison also indicated that the isolates that recovered from the non-febrile stage were contained in nucleotide insertions in the p...
A modified teniotomy technique for facilitated removal of descending colon enteroliths in horses. To describe a method for facilitating movement of enteroliths obstructing the proximal portion of the descending colon. Methods: Prospective report. Methods: 15 horses. Methods: A seromuscular incision through the antimesenteric taenia of the proximal portion of the descending colon was used to facilitate movement of enteroliths. Results: Teniotomy allowed proximal descending colon enteroliths located within the abdomen or close to the abdominal wall to be advanced 4 to 15 cm in an aboral direction so that they could be exteriorized for safe removal. Conclusions: Teniotomy is a feasible method...
Closed one-stage functional end-to-end jejunojejunostomy in horses with use of linear stapling equipment. To use gastrointestinal linear stapling instrumentation to perform a closed one-stage functional end-to-end jejunojejunostomy in adult horses as an alternative to a stapled side-to-side jejunojejunostomy. Methods: Clinical outcome, anatomic and histological architecture, and anastomotic dimensions were determined at 2, 4, 8, 16, and 24 weeks postoperatively. Methods: 18 adult horses. Methods: The anastomosis and peritoneal cavity were inspected for adhesions. The diameter and circumference of the anastomosis and jejunum oral and aboral to the anastomosis were determined from digitized contrast...
In vivo evaluation of intramedullary interlocking nail fixation of transverse femoral osteotomies in foals. To evaluate an intramedullary interlocking nail for stabilization of transverse femoral osteotomies in foals. Methods: A transverse osteotomy and restabilization with an intramedullary interlocking nail was performed on the right femur in three foals and the left femur in three foals. Methods: Six foals weighing 149 to 207 kg. Methods: The femur was destabilized with a transverse middiaphyseal osteotomy and repaired with a 0.5-in (12.7 mm) interlocking nail. The implanted femurs were radiographed monthly until completion of the study 6 months after surgery. At the completion of the study, all ...
Biomechanical comparison of the Herbert and AO cortical bone screws for compression of an equine third carpal bone dorsal plane slab osteotomy. To assess feasibility of insertion of 4.5-mm Herbert cannulated bone screws (HS) using fluoroscopic guidance and compare the mechanical shear strength of these HS and 4.5-mm AO cortical bone screws (AO) for fixation of dorsal plane slab osteotomies in equine cadaver third carpal bones (C3). Methods: Eight equine cadavers. Methods: Bone mineral composition and density of contralateral C3 were confirmed to be equivalent using dual-energy x-ray absorptiometry. A standard 10-mm C3 slab osteotomy was reduced using HS or AO instrumentation under fluoroscopic guidance. Specimens were loaded in shear ...
Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples. In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.
Fructosamine measurement in ponies: validation and response following experimental cyathostome infection. Validation of an assay for measurement of fructosamine in equine serum and plasma utilised blood samples collected from 24 British native breed ponies. The results indicated that fructosamine can be measured easily using an assay which is precise and accurate. Paired plasma and serum fructosamine measurements were highly correlated, however, greater variations were observed within serum compared with those in plasma. A reference range for fructosamine in plasma was calculated to be 256.9 +/- 60.6 mumol litre-1 (mean +/- 2 SD). In order to assess the fructosamine response following experimental...
Kinematic symmetry index: a method for quantifying the horse locomotion symmetry using kinematic data. This study was conducted to evaluate a method for quantifying locomotion symmetry at trot in sound and lame horses. Using a 3D kinematic analysis system, the kinematics of the limb joints of sound and lame horses were recorded. The differences in motion between the left and right homologous joints were then quantified using a symmetry index based on an inter-correlation method. This method was used to calculate the symmetry indices of the vertical displacements and angle-time diagrams of the joints of each horse. In order to evaluate the sensitivity of this method of quantifying the locomotion...
