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Topic:Diagnostic Technique
Diagnostic techniques in equine medicine encompass a range of procedures and tools used to identify diseases, injuries, or other health conditions in horses. These techniques include imaging methods such as radiography, ultrasonography, and magnetic resonance imaging (MRI), as well as laboratory tests like blood work and tissue biopsies. Each diagnostic method provides specific information that can aid in the assessment and management of equine health issues. Radiography is commonly used for evaluating bone structures, while ultrasonography is useful for soft tissue examination. MRI offers detailed images of both soft and hard tissues, though its use is limited by cost and availability. Laboratory tests can detect biochemical and hematological changes indicative of disease. This page compiles peer-reviewed research studies and scholarly articles that explore the development, application, and efficacy of various diagnostic techniques in equine veterinary practice.
Effects of denervation of the digit of the horse. A study was conducted in an effort to explain why digital necrosis sometimes follows neurectomy in the horse. Six horses were subjected to unilateral section of the medial and lateral palmar nerves. Arteriography was done on each digit prior to surgery. Terminally, arteriography was repeated. Sections of bone, nerve, artery, skin, coronary band, and deep flexor tendon were examined histologically. Changes in arterial pattern and bone quality were noted. During the study, 2 of the horses had clinical signs of digital necrosis. The composite findings suggested trauma or infection, or both, of th...
Serologic responses of pregnant thoroughbred mares to vaccination with an inactivated equine herpesvirus 1 vaccine. The immunogenic potency and safety of a chemically inactivated equine herpesvirus 1 vaccine with added adjuvant was evaluated by testing serum-neutralizing and complement-fixation antibody responses of pregnant Thoroughbred mares. The vaccinated population comprised 321 pregnant mares on 7 farms; 3 in Normandy, France; 1 in Kildare, Ireland; and 3 in central Kentucky. The pattern of antibody response to vaccination was found qualitatively and quantitatively similar to that of pregnant mares previously vaccinated and determined by challenge exposure to be immune to abortigenic infection under e...
Comparison of milk and blood plasma progesterone concentrations in cycling and pregnant mares. Progesterone concentrations were measured in milk and blood plasma for 15 mares throughout a normal estrous cycle and early pregnancy to determine the feasibility of utilizing progesterone in milk as an indicator of pregnancy. Samples were obtained daily from foaling until diagnosis of pregnancy by rectal examination at 30 to 35 days of gestation. Progesterone in milk and blood plasma was quantified by radioimmunoassay. Mean progesterone concentrations (+/- SE) in plasma from foaling to foal heat and during estrus, luteal phase and pregnancy were .51 +/- .09 ng/ml, .53 +/- .08 ng/ml, 3.88 +/- ...
Quantitative epidemiological studies on the prevalence of babesiosis in horses in Kuwait. Racehorses imported into Kuwait were tested for serum antibodies to Babesia equi and B. caballi by complement fixation (CF) and indirect fluorescent antibody (IFA) tests. The prevalence of B. equi was high (77.1% by IFA) whereas that of B. caballi was much lower (11.4%). Data for B. equi showed that infection was acquired after about 6 months at risk and that in the following 12 months animals had antibody levels detectable by both CF and IFA tests by 24 months the CF reaction was no longer detectable. Estimates of incidence and inoculation rates were calculated and found to be consistent with...
Soft tissue masses in the fetlock joint of horses. Eight cases of sessile, intra-articular soft tissue masses originating from the cranio-dorsal attachment of the capsule of the fetlock joint of horses are presented. In 4 of these cases an osteochondral fracture of the first phalanx was also present. Clinically the condition closely resembled villonodular synovitis; however the microscopic changes did not correspond to those reported. The clinical signs included lameness after exercise, joint effusion and pain on manipulation. The offending masses were surgically resected and their histology indicated a chronic proliferative synovitis. The res...
Selected ion monitoring assay for bromhexine in biological fluids. A method has been developed for quantification of bromhexine in plasma using gas chromatography mass spectrometry with selected ion monitoring. A deuterium labelled analogue was synthesized and used as the internal standard. To evaluate the gas chromatographic electron capture detection method described earlier, 23 plasma samples have been analysed by both techniques. Although a good correlation was shown, selected ion monitoring was superior to the electron capture detection method for levels below 3 ng ml-1. The mass spectrometric method has also been used to set up a pharmacokinetic study o...
Sequence of the low activity equine erythrocyte carbonic anhydrase and delineation of the amino acid substitutions in various polymorphic forms. the sequence of the low activity form of equine erythrocyte carbonic anhydrase has been determined. The most common electrophoretic form, designated D, has been found to have five substitutions. Amino acid exchanges in the electrophoretic variants known as A1, A2, B, and T have been found at six other positions. The data do not permit calculation of the number of polymorphic forms of this enzyme. The equine D isozyme and the analogous human enzyme are quite homologous, 211 of their 260 residues, or 81%, being identical.
Disorders of the thoracolumbar spine of the horse–a survey of 443 cases. The survey comprised 443 horses, referred to the clinical department of the Equine Research Station, with a history of a thoracolumbar (TL) complaint. A wide range of lesions were capable of producing back problems and more than one condition or site of injury was found in 75 animals (17 per cent). There were 103 horses (19.7 per cent) with no evidence of damage to the TL spine or its associated structures. In 66 of these cases, clinical signs were attributed to a variety of hindlimb lamenesses and, in 37, no specific diagnosis could be made. Soft tissue injury was diagnosed in 203 cases (38.8...
Assessment of wall shear stress in arteries, applied to the coronary circulation. Time dependent wall shear rates cannot be directly or accurately measured in arteries using presently available techniques. Here a simple method is presented for calculating them from a single measured velocity waveform (either centreline or cross-sectionally averaged velocity). The method involves only Fourier analysis and the application of given formulae, and it is expected to be approximately valid in any segment of artery which has no branches or sharp curves for a distance of several diameters. It is shown, however, that a frequency response of 30 Hz is required in the velocity measuring...
Hypogammaglobulinaemia in foals: prevalence on Victorian studs and simple methods for detection and correction in the field. The prevalence of hypogammaglobulinaemia in 82 young foals was determined. Twelve foals were considered clinically abnormal at birth and ten died within two weeks. All of these foals were hypogammaglobulinaemic. Seven (10%) of the other 70 apparently normal foals were hypogammaglobulinaemic despite having suckled normally. Three of these foals developed significant disease and one died at one month of age. Rapid detection of foals with low serum immunoglobulin levels was achieved by adapting the zinc sulphate turbidity test to partially evacuated blood collection tubes. This permitted test to ...
A survey of Clostridium perfringens enterotoxin antibody in human and animal sera in western Canada. Sera from human, cattle, sheep, swine, and horse populations in western Canada were tested for the presence of Clostridium perfringens enterotoxin antibody by the passive hemagglutination (PHA) test, supplemented by an immunodiffusion test and by counterimmunoelectrophoresis. A total of 224 human, 345 cattle, 165 sheep, 620 swine, and 768 horse serum samples were examined. Low-titer reactions in the PHA test were detected in human, cattle, horse, and swine sera, in that order, with no titers demonstrated in sheep. The titers in human sera ranged up to 1:128 and three of these samples were also...
Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA. Bovine papilloma virus (BPV) appears to be the etiological agent of common equine connective tissue tumors. We investigated the physical state of the viral DNA within such tumors and found no indication for integration into the host genome. The BPV genomes were present as free circular episomes. Two equine sarcoids were shown to contain multiple copies of free circular BPV type 1 (BPV-1) DNA. When the tumors were digested with several single-cut restriction enzymes, there were only form III BPV-1 DNA sequences could be revealed. One of the sarcoids contained, apart from wild-type BPV-1 DNA, a ...
Thermography in the diagnosis of inflammatory processes in the horse. To evaluate the use of thermography in equine medicine, a three-phase study was conducted. In the first phase, six horses were examined thermographically, before and after exercise, to determine a normal thermal pattern. In the second phase, nine horses with acute and chronic inflammatory processes were examined thermographically. In the third phase, thermography was used to evaluate the effectiveness of anti-inflammatory drugs on chemically induced inflammatory reactions. All normal horses tested had similar infrared emission patterns. There was a high degree of symmetry between right and lef...
Pyloric stenosis in a foal. A two month old Thoroughbred filly was presented with signs of depression, grinding of the teeth, frothing of the mouth and abdominal pain. These signs had persisted for two weeks despite treatment with mineral oil, dioctyl sodium sulfosuccinate, meperidine and antibiotics. A variety of diagnostic tests were done, the only abnormal finding was a stress leukon. On exploratory laparotomy the stomach was dilated with fluid and gas and the pyloric canal was constricted. Pyloroplasty resulted in correction of the condition. The etiological possibilities are discussed. This is believed to be the fir...
Thermography: a technique for subclinical diagnosis of osteoarthritis. Thermographic and radiographic evaluations of the tarsus (hock) were done on 20 Standardbred racehorses before and after exercise at three consecutive 6-week intervals. All horses were from the same stable and given the same care and training under identical schedules and conditions. Normal thermographic patterns were established before and after exercise. These patterns corresponded to the underlying tarsal vasculature. Postexercise thermal patterns indicated a warming trend, and the increases were uniform. Abnormal thermal patterns were more localized and did not conform to the normal underl...
Limitations in equine fetal electrocardiography. Technical and interpretive limitations of equine fetal electrocardiography were evaluated in recordings obtained from 45 pregnant mares. Technical limitations were related to the small amplitude of the fetal electrocardiogram and the variability in the lead configuration providing the best recording. It was found that recording the fetal electrocardiogram at high sensitivity and high base-line fidelity in several different leads was necessary to obtain satisfactory tracings. Interpretive limitations were related in part to the small amplitude of the fetal electrocardiogram and to the marked va...
