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Topic:Horses

"Horses" is a broad topic that encompasses various aspects of equine biology, behavior, and management. This category includes studies on the anatomy, physiology, and genetics of horses, as well as their behavior, nutrition, and care. Research in this area may also cover the historical and cultural significance of horses, their roles in agriculture, sport, and therapy, and the challenges associated with their conservation and welfare. The page aggregates peer-reviewed research articles and scholarly studies that explore the multifaceted relationships between humans and horses, examining both scientific and socio-economic perspectives.
Methods for the Detection of Trichinellosis in Horses.
Journal of food protection    April 1, 1996   Volume 59, Issue 4 420-425 doi: 10.4315/0362-028X-59.4.420
Gamble HR, Gajadhar AA, Solomon MB.Twelve horses were infected with various doses of Trichinella spiralis and then tested for infection using direct (artificial digestion) and indirect (enzyme immunoassay) methods. Horses became infected in a dose-dependent manner. Larvae accumulated preferentially in the tongue, followed by the masseter, neck, supraspinatus, trapezius, and diaphragm. At lower infection levels, the tongue harbored several times more parasites than were found in other tissues. The sensitivity of artificial digestion methods for detecting infections was directly related to sample size. One-gram samples were not r...
[Acute pain in the horse and one possibility for its objective evaluation].
Tierarztliche Praxis    April 1, 1996   Volume 24, Issue 2 108-112 
Zierz J, Wintzer HJ.To judge acute processes of pain objectively the results are told of a determination of adrenaline and noradrenaline in the plasma of 30 horses suffering from pain. Besides a scheme basing on an awarding of points is developed to ascertain changes of physiological and ethological parameters caused by pain. These results in changes of behaviour are compared to results determined by laboratory experiments. Concerning pain of medium and high level a relation to the concentration of catecholamines is noticed. Therefore the total of certain clinical observations is suitable for graduating acute pai...
Immunohistolocalization of the carbonic anhydrase isoenzymes (CA-I, CA-II, and CA-III) in the reproductive tract of male horses.
American journal of veterinary research    April 1, 1996   Volume 57, Issue 4 439-443 
Asari M, Sasaki K, Miura K, Ichihara N, Nishita T.To elucidate locations of cytosolic carbonic anhydrase isoenzyme (CA-I, CA-II, and CA-III)-positive epithelial cells in equine male reproductive organs. Methods: Descriptive and immunohistochemical study. Methods: 4 clinically normal male horses. Methods: The testis (seminiferous tubules, rete tubules), epididymis (initial, middle, and terminal segments), proximal and distal portions of the ductus deferens, ampulla ductus deferentis, seminal vesicle, prostate, and bulbourethral gland were excised from euthanatized horses after administration of an overdose of pentobarbital. The tissue specimen...
Characterization of two polymorphic horse microsatellites: HMS15 and HMS20.
Animal genetics    April 1, 1996   Volume 27, Issue 2 123 
Guérin G, Bertaud M.No abstract available
Maturation and fertilization of equine oocytes.
The Veterinary clinics of North America. Equine practice    April 1, 1996   Volume 12, Issue 1 31-45 doi: 10.1016/s0749-0739(17)30293-6
Squires EL.Equine oocytes obtained either by transvaginal ultrasound-guided follicular aspiration or from slaughterhouse ovaries can be matured in vitro. This generally requires culture in TCM-199 containing serum and hormones for 30 to 36 hours. With this protocol, approximately 50% to 60% of the oocytes are at metaphase-II at the end of the culture period. At least some of these oocytes appear viable based on production of fertilized eggs either through in vitro fertilization or fertilization in vivo of a recipient mare. The success of producing equine embryos in vitro is still extremely low. More than...
Local distribution of mepivacaine after distal interphalangeal joint injection in horses.
American journal of veterinary research    April 1, 1996   Volume 57, Issue 4 422-426 
Keegan KG, Wilson DA, Kreeger JM, Ellersieck MR, Kuo KC, Li Z.To evaluate the distribution of mepivacaine hydrochloride after distal interphalangeal (DIP) joint injection in horses. Methods: Prospective, uncontrolled study. Methods: 10 adult horses. Methods: 30 minutes before euthanasia, 8 ml of 2% mepivacaine hydrochloride was injected into the dorsal pouch of a forelimb DIP joint. Synovial tissue from the DIP joint and podotrochlear (navicular) bursa and bone tissue from the medullary cavity of the distal sesamoid (navicular) bone were taken from both forelimbs immediately after death. All synovial and bone specimens were analyzed for tissue concentrat...
Methods for induction of capacitation and the acrosome reaction of stallion spermatozoa.
The Veterinary clinics of North America. Equine practice    April 1, 1996   Volume 12, Issue 1 111-117 doi: 10.1016/s0749-0739(17)30298-5
Graham JK.Methodologies to capacitate bovine spermatozoa, induce the acrosome reaction, and fertilize bovine oocytes in vitro have been established. The capability to do the same with stallion spermatozoa, however, is not available. Several different methods have been used to capacitate stallion spermatozoa with variable results. More basic research needs to be done to establish in vitro conditions necessary to capacitate and induce an acrosome reaction in stallion spermatozoa. Although much progress can be expected in this area, it is unlikely that the general practitioner will use these technologies i...
GnRH therapy for subfertile stallions.
The Veterinary clinics of North America. Equine practice    April 1, 1996   Volume 12, Issue 1 149-160 doi: 10.1016/s0749-0739(17)30301-2
Brinsko SP.Poor fertility has been associated with hormonal imbalances in the stallion. The primary cause of this imbalance is unknown, but it may be related to abnormal gonadotropin secretion. Secretion of GnRH from the hypothalamus causes the release of LH and FSH from the anterior pituitary and these gonadotropins help maintain adequate steroid levels so that normal spermatogenesis can occur. Hypogonadotropic hypogonadism has not been documented in the stallion; however, GnRH therapy has improved the fertility of a limited number of horses. Downregulation of the HPT axis readily occurs in most other s...
Equine dinucleotide repeat polymorphisms at loci LEX002, -003, -004, -005, -007, -008, -009, -010, -011, -013 and -014.
Animal genetics    April 1, 1996   Volume 27, Issue 2 126-127 
Coogle L, Bailey E, Reid R, Russ M.No abstract available
Ultrasonographic renal changes associated with phenylbutazone administration in three foals.
The Canadian veterinary journal = La revue veterinaire canadienne    April 1, 1996   Volume 37, Issue 4 235-236 
Léveillé R, Miyabayashi T, Weisbrode SE, Biller DS, Takiguchi M, Williams JF.No abstract available
Disposition and excretion of 6-methoxy-2-naphthylacetic acid, the active metabolite of nabumetone in horses.
American journal of veterinary research    April 1, 1996   Volume 57, Issue 4 517-521 
Soma LR, Uboh CE, Rudy JA, Smith MS.To examine, in horses, the disposition and excretion of the active metabolite 6-methoxy-2-naphthylacetic acid (6MNA) of the nonsteroidal anti-inflammatory prodrug nabumetone. Methods: Pharmacokinetic analysis of 6MNA after oral administration of nabumetone and IV administration of 6MNA. Methods: Using a crossover design, 5 horses were orally administered 3.7 mg of nabumetone/kg of body weight. After a 3-week washout period, 4 horses were administered 2.5 mg of 6MNA/kg, IV. Results: Absorption of nabumetone from the gastrointestinal tract and its metabolism to 6MNA had a median appearance half-...
Prothipendyl: detection and elimination in the horse–a case report.
DTW. Deutsche tierarztliche Wochenschrift    April 1, 1996   Volume 103, Issue 4 125-127 
Hagedorn HW, Zuck S, Schulz R.The azaphenothiazine neuroleptic prothipendyl (Dominal) is suspected to be administered illegally at low doses to race-horses to improve their performance. Since for this species pharmacokinetic data of the drug are missing we studied its elimination from blood and urine in a standard-bred mare. At a low (subtherapeutic) dose (i.v., 0.24 mg/kg) the horse is described to be less excited while locomotor activity and attention remain unaffected. In contrast, sedation and ataxia are brought about at 1 mg/kg (therapeutic dose). Identification of prothipendyl given i.v. at subtherapeutic doses was a...
[Expert opinions about a case of injury which by a failure of a restraining device caused a life-threatening injury to a breeding stallion].
Tierarztliche Praxis    April 1, 1996   Volume 24, Issue 2 113-116 
Merkt H.A stallion got fatal injuries by kicks of a maiden mare because a so called "panic hook" untied spontaneously. The use of such hooks to secure mares during mating should therefore not be recommended.
The effects of bovine somatotropin (bST) and porcine somatotropin (pST) on growth factor and metabolic variables in horses.
Journal of animal science    April 1, 1996   Volume 74, Issue 4 886-894 doi: 10.2527/1996.744886x
Buonomo FC, Ruffin DS, Brendemeuhl JP, Veenhuizen JJ, Sartin JL.Effects of exogenous pST and bST on metabolic and growth factor variables were examined in three studies with lighthorse mares (455 to 545 kg). In Study 1, eight mares received five s.c. injections of bST or pST (30 mg/d). In Studies 2 and 3, five mares received one s.c. injection of a prolonged release formulation designed to deliver 500 mg of bST (Study 2) or pST (Study 3) over 14 d. Blood samples were collected for several days before injection to establish baseline values, at frequent intervals during treatment, and for several days thereafter. In all studies, blood urea nitrogen concentra...
Mycobacterium avium complex abortion in a mare. Hélie P, Higgins R.No abstract available
Transvaginal aspiration.
The Veterinary clinics of North America. Equine practice    April 1, 1996   Volume 12, Issue 1 13-29 doi: 10.1016/s0749-0739(17)30292-4
Squires EL, Cook NL.This article describes in detail the procedures for collection of equine oocytes using a transvaginal ultrasound probe. Success in obtaining oocytes from humans, bovines, and horses are presented. The effect of repeated follicular aspiration of both cattle and horses is reviewed.
Ctenocephalides felis flea infestation in horses.
Veterinary parasitology    April 1, 1996   Volume 62, Issue 3-4 341-343 doi: 10.1016/0304-4017(95)00889-6
Yeruham I, Rosen S, Braverman Y.The horses were in two stables: in the first stable they were kept together with sheep and in the second stable they were kept together with goats. All horses in both stables were diagnosed as being infested with the cat flea Ctenocephalides felis felis. Foals were severely infested, whereas adult horses were only moderately infested.
Evaluation of propofol for general anesthesia in premedicated horses.
American journal of veterinary research    April 1, 1996   Volume 57, Issue 4 512-516 
Mama KR, Steffey EP, Pascoe PJ.To evaluate selected hemodynamic, respiratory, and behavioral responses to propofol in horses premedicated with xylazine or detomidine. Methods: Xylazine (0.5 and 1.0 mg/kg of body weight) was administered IV on different days to each of 6 horses prior to IV administration of propofol (2 mg/kg). In a second group of 6 horses, detomidine (15 and 30 micrograms/kg) was similarly studied. Methods: 2 groups of 6 mature healthy horses. Methods: Rectal temperature, heart and respiratory rates, arterial blood gas tensions, and direct arterial blood pressures were recorded before and at fixed intervals...
Traumatic rupture of the urinary bladder in a horse.
Australian veterinary journal    April 1, 1996   Volume 73, Issue 4 154-155 doi: 10.1111/j.1751-0813.1996.tb10010.x
Beck C, Dart AJ, McClintock SA, Hodgson DR.No abstract available
Early embryonic development and evaluation of equine embryo viability.
The Veterinary clinics of North America. Equine practice    April 1, 1996   Volume 12, Issue 1 61-83 doi: 10.1016/s0749-0739(17)30295-x
Vanderwall DK.Tremendous progress has been made in the development of assisted reproductive techniques that may enhance the reproductive efficiency of the horse. However, techniques that involve the manipulation of oocytes and/or embryos may themselves be detrimental to embryo viability and subsequent development. Therefore, an objective method of assessing viability of embryos before and/or after oocyte/embryo manipulation is desirable. At this time, morphologic evaluation is the most widely used method of determining the viability of equine embryos. Although morphologic assessment of embryo quality will n...
[Kidney function tests in horses–methods and reference values in healthy animals].
DTW. Deutsche tierarztliche Wochenschrift    April 1, 1996   Volume 103, Issue 4 117-122 
Bickhardt K, Deegen E, Espelage W.Investigations of renal function have been done on the basis of renal clearance (Clr) and excretion (E) of endogenous creatinine in healthy horses of different body weight (23 female, 7 male). Creatinine was measured by enzymatic PAP-method. Creatinine-E of female horses was poor positively correlated with body weight. The creatinine-Clr was highly correlated with the inulin-Clr (r = 0,896, p < 0,001). The excretion of creatinine was 35% higher than the filtration of creatinine, determined by inulin-Clr. That demonstrate a tubular net secretion of 26% of the total creatinine excretion. However...
Scintigraphic evaluation of 99mTc-methylene diphosphonate uptake in the navicular area of horses with lameness isolated to the foot by anesthesia of the palmar digital nerves.
American journal of veterinary research    April 1, 1996   Volume 57, Issue 4 415-421 
Keegan KG, Wilson DA, Lattimer JC, Twardock AR, Ellersieck MR.To evaluate distribution and intensity of 99mTc-methylene diphosphonate (99mTc-MDP) uptake in the navicular area in horses with forelimb lameness isolated to the palmar aspect of the foot. Methods: Prospective, case-controlled study. Methods: 7 horses with clinical signs of navicular syndrome and 7 control horses. Methods: Palmar view, soft tissue-phase scintigraphic images of the foot were obtained between 7 and 12 minutes after injection of 120 to 170 mCi of 99mTc-MDP. Lateral and palmar view, bone-phase images were obtained at 30 minutes and 1, 2, and 4 hours after injection. Palmar views w...
Hydrocele formation after castration in 3 geldings.
Australian veterinary journal    April 1, 1996   Volume 73, Issue 4 156-157 doi: 10.1111/j.1751-0813.1996.tb10011.x
Colbourne CM, Adkins AR, Yovich JV.No abstract available
Equine neonatal septicaemia: 24 cases.
Australian veterinary journal    April 1, 1996   Volume 73, Issue 4 137-140 doi: 10.1111/j.1751-0813.1996.tb10006.x
Raisis AL, Hodgson JL, Hodgson DR.Equine neonatal septicaemia was confirmed in 24 foals hospitalised at the Rural Veterinary Centre between 1989 and 1992 with suspected septicaemia. Septicaemia was confirmed by culture of bacteria from blood of live foals and tissues obtained at necropsy of foals that died or were euthanased. Pathogenic bacteria isolated were predominantly Enterobacteriaceae (including Escherichia coli and Salmonella serovars) and Actinobacillus equuli. Clinical manifestations of septicaemia included signs of depression, dehydration, abnormalities in body temperature and manifestations of localised infection i...
Biological and imaging characteristics and radiation dose rates associated with the use of technetium-99m-labelled imidodiphosphate in the horse. Riddolls LJ, Byford GG, McKee SL.The biological and imaging characteristics of technetium-99m imidodiphosphate (Tc99m-IDP) were measured in 4 horses once and in 1 horse twice. All computational results are expressed with 95.5% (mean +/- 2 SD) confidence limits. The clearance half-time of the radiopharmaceutical from the blood was 29.6 +/- 2.3 min. The percentage of the administered dose circulating in the whole-blood volume at 4 h was 3.9 +/- 0.8%. The Tc99m-IDP radioactivity confined at the plasma fraction of the whole blood at 4 h was 85.3 +/- 1.6%. At 8 h, approximately 45 +/- 16% of the dose administered had been excreted...
Zoo-FISH delineates conserved chromosomal segments in horse and man. Raudsepp T, Frönicke L, Scherthan H, Gustavsson I, Chowdhary BP.Human chromosome specific libraries (CSLs) were individually applied to equine metaphase chromosomes using the fluorescence in situ hybridization (FISH) technique. All CSLs, except Y, showed painting signals on one or several horse chromosomes. In total 43 conserved chromosomal segments were painted. Homoeology could not, however, be detected for some segments of the equine genome. This is most likely related to the very weak signals displayed by some libraries, rather than to the absence of similarity with the human genome. In spite of divergence from the human genome, dated 70-80 million yea...
Enzyme-linked immunosorbent assay for thrombin-antithrombin III complexes in horses.
American journal of veterinary research    April 1, 1996   Volume 57, Issue 4 427-431 
Topper MJ, Prasse KW, Morris MJ, Duncan A, Crowe NA.To adapt and characterize a human ELISA kit to quantify thrombin-antithrombin III (TAT) complexes in horses, and to evaluate TAT as a marker for hypercoagulation in horses. Methods: 29 clinically normal horses used as controls, and 4 ill horses used to evaluate assay for known causes of hypercoagulation. Methods: A commercially available human sandwich-type ELISA kit with 2 antibodies against human thrombin and antithrombin III that bind selectively to their corresponding TAT antigenic sites was used. Equine TAT standards were made from purified equine thrombin and antithrombin III. Proteins d...
Viability of split-thickness skin grafts attached with fibrin glue. Schumacher J, Ford TS, Brumbaugh GW, Honnas CM.Full-thickness, circular, cutaneous wounds (4 cm diameter) were created on metacarpi and metatarsi of 5 horses. On day 6, all 4 wounds on each horse received a stored autogenous split-thickness sheet graft. Grafts were obtained from the horse's ventrolateral thorax with a pneumatic dermatome at the time the cutaneous wounds were created. Grafts were coapted to the granulation bed of 2 wounds of each horse with fibrin glue. Grafts were coapted to the cutaneous margin of all 4 wounds of each horse with cyanoacrylate glue. Bandages were changed daily until the study ended at 14 d. When the bandag...
Joint pressure influences synovial tissue blood flow as determined by colored microspheres.
Journal of applied physiology (Bethesda, Md. : 1985)    April 1, 1996   Volume 80, Issue 4 1225-1232 doi: 10.1152/jappl.1996.80.4.1225
Hardy J, Bertone AL, Muir WW.We measured regional blood flow in synovial tissue of the antebrachiocarpal, midcarpal, and metacarpophalangeal joints of six normal adult anesthetized horses by using 15-microns-diameter polystyrene colored microspheres. The midcarpal fibrous capsule and synovial membrane blood flows (SMBF) were compared, and the effect of increased intra-articular pressure (30 and 60 mmHg) on midcarpal SMBF was investigated. Dorsal, medial palmar, and lateral palmar midcarpal SMBF measured 108 +/- 36, 61 +/- 12, and 50 +/- 11 microliters.min-1.g-1, respectively. Antebrachiocarpal, dorsal, and palmar metacarp...
Cloning and sequencing of an equine insulin-like growth factor I cDNA and its expression in fetal and adult tissues.
General and comparative endocrinology    April 1, 1996   Volume 102, Issue 1 11-15 doi: 10.1006/gcen.1996.0040
Otte K, Rozell B, Gessbo A, Engström W.A cDNA for equine insulin-like growth factor I (IGF I) has been isolated by reverse transcriptase-polymerase chain reaction and subsequently sequenced. The sequenced fragment contained 465 bp including the coding regions for the signal peptide, the entire mature protein, and 4 amino acids into the E-peptide. Like its human counterpart, the mature equine IGF I peptide contains 70 amino acids and was 100% homologous between horse and man. The 49-amino-acid signal peptide had the threonine in position 26 of the human signal peptide substituted by isoleucine. The nucleotide homology across the ent...