The physiology of horses encompasses the study of the biological functions and processes that occur within the equine body. This includes the examination of various systems such as the cardiovascular, respiratory, musculoskeletal, digestive, and nervous systems. Understanding equine physiology is essential for comprehending how horses adapt to different environmental conditions, perform physical activities, and respond to health challenges. Research in this field often focuses on the mechanisms of energy metabolism, thermoregulation, and muscle function during exercise, as well as the physiological responses to stress and disease. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine physiology, providing insights into the biological processes that support the health and performance of horses.
Hernández-Vidal G, Jeffcott LB, Davies ME.A polyclonal antiserum raised in sheep against human cathepsin B was tested for specificity and cross-reactivity with the horse homologue by SDS-PAGE and Western blotting, prior to being used for immunolocalization of the enzyme in equine articular cartilage. In Western blots, the antiserum recognized the 30 kDa single chain and 25 kDa heavy chain of the mature enzyme in purified bovine cathepsin B, and corresponding bands at 32 and 27 kDa in equine chondrocyte and fibroblast lysates. This antiserum was then used to compare the expression and distribution of cathepsin B in normal and dyschondr...
Lee YH, Clarke KW, Alibhai HI.The effects on intramuscular blood flow and cardiopulmonary parameters of changing from anaesthesia with halothane to isoflurane and vice versa were investigated in six ponies (small horses). Anaesthesia was induced with xylazine, ketamine and diazepam, maintained for one hour with halothane at an end tidal concentration of 1 per cent and then with isoflurane at 1.5 per cent for a further hour (halo/iso). On another occasion the order in which the volatile agents were administered was reversed (iso/halo). After one hour of anaesthesia the mean (sd) arterial blood pressure (MAP) and cardiac out...
Kotsiubenko AA.The indexes of standardized deviations by the measured characters (probits) are expedient to be used for distribution of horses according to classes M-, M0 and M+, under these conditions one can select individuals M0 for to rise their capacity for work and expressivity of the type of breed.
Overesch G, Wagner B, Radbruch A, Leibold W.The number of immunoglobulin G constant heavy chain genes (cgamma genes) varies broadly among mammalian species, reflecting structural and functional differences between expressed immunoglobulin G (IgG) isotypes and allotypes. Up to now equine IgG isotypes have been defined only at the biochemical and serological level. It is still not clear how many IgG isotypes exist in horses and whether there are any allotypes. Here, we describe the isolation and characterisation of equine cgamma genes. An equine genomic lambda phage library was screened with a human cgamma4 probe. Cross-hybridising equine...
Smith DJ, Iqbal J, Purewal A, Hamblin AS, Edington N.IL-2 and equine chorionic gonadotrophin (eCG) initiated reactivation of equid herpesvirus-1 (EHV-1) from venous lymphocytes at a frequency of 1/10(-5). Indirect immunofluorescence showed that > 80% of virus-positive leukocytes were CD5+/CD8+ with the remaining 20% being CD5+/CD8-/CD4-. Cocultivation demonstrated that the reactivated virus was infectious. In addition, virus was reactivated in vitro from leukocytes of > 70% of horses by the mitogens phytohaemagglutinin (PHA) and pokeweed mitogen (PWM). Transfer of supernatants showed that IL-2 and eCG acted indirectly by causing the releas...
Lin G, Chen GH, Ho HC.Conformationally restricted carbamate inhibitors, exo-2-norbornyl-N-butylcarbamate (1), endo-2-norbornyl-N-butylcarbamate (2), l-adamantyl-N-butylcarbamate (3), and 2-adamantyl-N-butylcarbamate (4) as active site-directed irreversible inhibitors of horse serum butyrylcholinesterase are investigated for values of the dissociation constant (KI), the carbamylation constant (k2), and the bimolecular rate constant (ki). Compound 1 is the most potent inhibitor of the enzyme and the values of KI and ki are 20 nM and 1.1 x 10(5) M-1sec-1, respectively.
King N, Suleiman MS.Exercise induces significant changes in the free intracellular amino acid pool in skeletal muscle but little is known of whether such changes also occur in cardiac muscle. In this study the effect of regular exercise on the size and the constituents of the free amino acid pool in the hearts and in the plasma of thoroughbred horses was investigated. The total free intracellular amino acid pool in the hearts of control horses was 30.9 +/- 1.2 mumol/g wet weight (n = 6). Glutamine but not taurine was present at the highest concentration (13.5 +/- 0.9 and 7.7 +/- 0.69 mumol/g wet weight for glutam...
Venugopalan CS, Beadle RE, Seahorn TL, Holmes EP.The response of parenchymal strips from guinea-pig lungs to tracheobronchial lavage fluid (TBLF) collected from 8 normal horses and from 8 affected with summer pasture-associated obstructive pulmonary disease (SPAOPD) was determined. TBLF was collected during the summer (July) and winter (February) seasons. The serum/TBLF urea nitrogen ratio was used to standardize the mediator concentration in the TBLF. Four strips were used from each guinea-pig. The first strip did not receive any antagonist and served as the control. The second, third and fourth strips received antagonists of PGE2, LTD4 and...
The Journal of nutritionDecember 30, 1998
Volume 128, Issue 12 Suppl 2698S-2703S doi: 10.1093/jn/128.12.2698S
Harris PA.In the first part of this century, there was little advancement in horse nutrition and little research was undertaken. However, in the last few years, there has been a great increase in interest in this whole area. This review surveys some of the more recent developments and how they have influenced feeding practices and also compares these with those feeding practices found at the start of this century. The review concentrates on the nutrition of the adult horse in work, exploring in particular what they are fed and how the nutrient value of these feeds is evaluated.
The Journal of nutritionDecember 30, 1998
Volume 128, Issue 12 Suppl 2704S-2707S doi: 10.1093/jn/128.12.2704S
Pagan JD, Harris P, Brewster-Barnes T, Duren SE, Jackson SG.Most digestibility studies in horses have been conducted with idle horses confined to metabolism stalls. The values obtained from such studies are used for all classes of horses, including the performance horse. It has not been clearly established whether exercise affects digestibility. Olsson and Ruudvere (1955), summarizing a number of earlier studies, suggested that digestion may be affected in horses by work or exercise in such a way that it is improved by light exercise and inhibited by heavy work.
Orton et al. (1985) reported reduced retention time of a particulate marker in yearling ...
Irvine CH, Turner JE, Alexander SL, Shand N, van Noordt S.In mares, dioestrous FSH profiles based on once-a-day sampling are variable; however, the pulsatility of plasma FSH, which has been suggested by limited windows of intensive sampling, may contribute to this variability. Jugular blood from six mares was sampled at 4 h intervals throughout an ovulatory cycle to determine cyclic FSH and LH patterns more accurately and to measure gonadotrophin pulse frequency during dioestrus. Synchronous pulses of FSH and LH occurred regularly in all mares between day 4 and day 12 (ovulation = day 0) with a mean (+/- SEM) frequency of 1.9 +/- 0.1 (FSH) or 1.6 +/-...
Starbuck GR, Stout TA, Lamming GE, Allen WR, Flint AP.Circulating concentrations of 13,14-dihydro-15-ketoprostaglandin F2 alpha (PGFM) were measured before and after administration of oxytocin and after endometrial biopsy, with or without uterine flushing performed per vaginam, on days 10, 14 and 18 after ovulation in nine pregnant and nine cyclic mares. Concentrations of oxytocin receptor were measured in endometrial biopsy samples. Neither pregnancy status nor time after ovulation affected basal PGFM concentrations. PGFM concentrations were increased after oxytocin administration on each of the days studied in cyclic mares; on day 14 the mean r...
Song L, Porter DG, Coomber BL.Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) play very important roles in extracellular matrix (ECM) remodeling in ovarian follicle growth and ovulation. Equine follicles are embedded in cortex that is at the center of the ovary, and they must expand/emigrate to the fossa, the only site in the ovary for ovulation. Therefore, equine ovarian stromal cells (EOSC) are probably involved in ECM remodeling during follicle growth. This study examined whether cultured EOSC synthesize gelatinases and TIMPs, molecules essential for ECM remodeling in other systems. Results showed...
Aguilera-Tejero E, Garfia B, Estepa JC, López I, Mayer-Valor R, Rodríguez M.To determine effects of exercise on blood ionized calcium (Ca2+) and plasma parathyroid hormone (PTH) concentrations in horses and to compare the effects of exercise-induced and EDTA-induced hypocalcemia on PTH secretion. Methods: 17 horses entered in a show jumping competition and 5 horses given EDTA. Methods: Blood Ca2+ and plasma PTH concentrations were measured before and after exercise in the 17 horses entered in the jumping competition. In the other 5 horses, concentrations were measured during infusion of EDTA IV. Results: Exercise resulted in a significant decrease in blood Ca2+ concen...
Ebert DW, Bertone AL, Roberts C.To determine whether irradiation with a low-intensity diode laser, which produces radiation at a wavelength of 810 nm, will induce nonthermal enhancement of chondrocyte metabolism. Methods: 144 grossly normal articular cartilage explants aseptically harvested from the femoral condyles of 6 adult horses. Methods: Treated cartilage explants were irradiated with a diode laser at 1 of 7 fluence levels that ranged from 8 to 1,600 J/cm2. Explants were incubated for 24 or 72 hours, labeled for 24 hours with [35S]Na2SO4, and assayed for newly synthesized sulfated glycosaminoglycan (GAG; measured incor...
Richardson DW, Dodge GR.To clone the entire coding sequence of equine matrix metalloproteinase-3 (MMP-3, stromelysin) and tissue inhibitor of metalloproteinase-1 (TIMP-1) and compare their nucleotide and amino acid sequences with those of MMP-3 and TIMP-1 from other species. Methods: Articular cartilage harvested from the joints of 4 foals, 2 yearlings, and 3 adult horses. Methods: A cDNA library was constructed from mRNA extracted from equine chondrocytes. The library was screened and clones selected that contained the cDNA for MMP-3 and TIMP-1. The cDNA was sequenced and the nucleotide and deduced amino acid sequen...
Sasaki N, Mizuno Y, Yoshihara T.Electrogastrography (EGG), in which the electrical activity of the smooth muscular layer of the stomach is recorded percutaneously through the abdominal wall, has been applied in recent years to humans as a non-invasive method. In acute abdominal disease in horses, it is considered diagnostically useful to analyze digestive activity using EGG. Electrocecography (ECG) was examined to determine its effectiveness in evaluating equine digestive motility through comparison, after xylazine administration, between the results of the percutaneous ECG method and the results obtained using a strain-gaug...
Vanin AF, Serezhenkov VA, Mikoyan VD, Genkin MV.The parameters of EPR signal from dinitrosyl-iron complexes (DNIC) with bovine serum albumin (BSA), horse hemoglobin (Hb), and apometallothionein (apo-Mt) of horse kidney incorporating one (BSA, Hb) or two thiol-containing ligands (apo-Mt) were compared. The EPR signal from DNIC-BSA was characterized by the rhombic symmetry of g tensor at room temperature of signal recording (ambient temperature) or at 77K in the solution frozen in the presence of glycerol. In freezing of the solution in the absence of glycerin, under the exposure of DNIC-BSA to negatively charged sodium dodecyl sulfate (SDS) ...
Bailey DN.The relative binding of acetaminophen, lidocaine, phenobarbital, procainamide, quinidine, and theophylline to sera of seven mammalian species was studied. Pooled commercial sera from cow, goat, horse, human, pig, rabbit, and sheep were supplemented with 5 and 10 mM concentrations of each drug. For each serum, each drug, and each drug concentration, equilibrium dialysis was performed in duplicate against phosphate buffer (pH 7.4, 0.1 M, 4 degrees C). Percent drug bound to serum was calculated. Phenobarbital demonstrated more than 20% binding to goat, horse, human, and sheep serum at both 5 and ...
Birch HL, Bailey AJ, Goodship AE.Injuries to the superficial digital flexor tendon are common in horses required to gallop and jump at speed. Partial rupture of this tendon usually occurs in the central core of the midmetacarpal region and may be preceded by localised degenerative changes. Post mortem examination of apparently normal equine flexor tendons has revealed an abnormal macroscopic appearance in the central core, characterised by a reddish discolouration. We have previously shown that there is also physical damage to the collagen fibres. In the present study we tested the hypothesis that the abnormal appearance is a...
Wilson AM, Seelig TJ, Shield RA, Silverman BW.Foot imbalance is believed to be a common cause of musculoskeletal injury in the horse; its biomechanical effects are, however, poorly understood. Wedges (angle 3.7 and 5 degrees) were attached to modified shoes to elevate one aspect of both front feet of Thoroughbred-type horses. The point of force application during weightbearing was determined at trot using a forceplate system. A total of 8 horses were studied with a minimum of 4 providing data for each wedge condition. The results demonstrated that application of a standard steel horse shoe to a balanced foot has minimal effect on the poin...
Estepa JC, Aguilera-Tejero E, Mayer-Valor R, Almadén Y, Felsenfeld AJ, Rodríguez M.Measurement of parathyroid hormone (PTH) in horses was performed on plasma samples using 2 immunoradiometric assays: a human intact PTH assay and a rat amino-terminal PTH assay. The assays were validated by assessment of their precision, sensitivity and specificity, and also by evaluating PTH changes in the horse in response to variation in blood ionised calcium. Intra- and inter-assay variance, precision and sensitivity were similar for both human and rat assays; however, the rat assay was slightly more precise and sensitive than the human assay. Both assays detected an increase in PTH levels...
Lantz KC, Enders AC, Liu IK.In addition to the unique feature of retention of unfertilized ova, the oviducts of mares frequently contain large intraluminal masses with a fibrillar component and some cells. The aim of this study was to identify the cells and examine their relationship to the extracellular components of these masses. Intraluminal masses were examined both in situ and flushed from the oviducts. The nature of the contained cells and their relationship to the fibrils were examined by light microscopy and by transmission and scanning electron microscopy. In some mares the large masses distended the oviduct, bu...
Bryant CE, Thompson J, Clarke KW.Medetomidine was administered to sheep and horses at a dose rate of 5 microg kg(-1) (i.v.). Heart rate and blood pressure were recorded. Medetomidine induced bradycardia and a biphasic blood pressure response consisting of a transient hypertension followed by hypotension. Administration of prazosin (an alpha1 adrenoceptor antagonist; 100 microg kg(-1), i.v.) had no effect on the cardiovascular response to medetomidine (5 microg kg(-1), i.v.), but inhibited the cardiovascular response of methoxamine (an alpha1 adrenoceptor agonist; 75 microg kg(-1), i.v.). L-659,066 (an alpha2 adrenoceptor anta...
Gray AW, Davies ME, Jeffcott LB.We report on preliminary results of a novel in vitro culture system designed to generate equine osteoclasts in large numbers. Osteoclast generation, as determined by the expression of tartrate resistant acid phosphatase (TRAP) and ability to resorb bone, was enhanced in equine bone marrow cultures supplemented with fibroblastic cell (L929) conditioned medium (L929-CM). Bone marrow was collected from a total of 12 horses and ponies and TRAP-positive cells with bone resorbing ability were generated in significant numbers in the last seven. TRAP-positive mononuclear cells appeared after three day...
Brodbelt DC, Harris J, Taylor PM.This study investigated whether maintenance of normotension using a methoxamine infusion would prevent activation of the stress response during halothane anaesthesia in ponies. After acepromazine premedication, anaesthesia was induced with thiopentone in six ponies, and maintained for 120 minutes with halothane in oxygen and ventilation to normocapnia. Methoxamine (M, 0.013+/-0.005 mg kg(-1) min(-1)) or saline (C) was infused throughout anaesthesia. Sequential blood samples were taken for cortisol and ACTH assay. During anaesthesia normotension (mean arterial blood pressure 101-124 mm Hg) was ...
Okumura M, Tagami M, Fujinaga T.Keratan sulphate (KS) concentration and anticollagen type II antibody levels were measured in the serum and synovial fluid (SF) of clinically normal horses and horses with osteoarthritis (OA). Serum KS in OA was significantly higher than that in normal horses, while no significant difference was found in KS levels of SF between normal and OA. Assays of antibody to collagen type II showed no significant increase in sera and SF of OA. It was suggested that levels of serum KS would be of value in the pathological detection of OA in the joint, although there was no evidence that the measurable aut...
Gauff FC, Patan-Zugaj B, Licka TF.To determine the effect of short-term hyperinsulinemia on the localization and expression of endothelin receptor (ETR)-A and ETR-B in lamellar tissue of the forelimbs of horses. Methods: Distal portion of 15 cadaveric forelimbs from healthy adult horses (1 limb/horse) obtained immediately after slaughter at an abattoir. Methods: Each forelimb was assigned to 1 of 3 treatment groups (perfused with autologous blood for 10 hours [control perfusion; n = 5], perfused with an insulin [142 ± 81 μU/mL] perfusate for 10 hours [insulinemic perfusion; 5], or not perfused [unperfused control; 5]). Immun...
Varhac R, Antalík M.A relation between pH-induced conformational transitions of horse heart ferricytochrome c and the kinetics of external ligand coordination to heme iron was investigated by optical spectroscopy, circular dichroism and viscometry. The dependencies of both the association, k (a), and dissociation rate constants of cyanide binding on pH were determined from kinetic measurements. The association rate constant exhibits a bell-shaped form of dependence on pH in the region where this protein unfolds. The maximum of the dependence of k (a) on pH is found to be coincident with the pK values of conformat...
Waller CA, Thompson DL, Cartmill JA, Storer WA, Huff NK.Previous results from our laboratory indicated that a majority of mares with high body condition scores (BCS) displayed estrous cycles or had considerable follicular activity during the winter. Among these high BCS mares, about 35% of them exhibited a persistent hyperleptinemia and hyperinsulinemia. The current experiment was designed to compare the reproductive characteristics of high BCS mares with hyperleptinemia to those with normal (low) plasma concentrations of leptin during the winter and the first estrous cycle (or the first full cycle encountered for those already cycling). Light hors...
Farley J, Sirois J, MacFarlane PH, Kombé A, Laverty S.To characterize expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and regulation of prostaglandin E2 (PGE2) production by equine articular chondrocytes. Methods: Articular cartilage from the metacarpophalangeal joints of 7 adult horses. Methods: Equine chondrocyte monolayer cultures were stimulated with different concentrations (2.5, 5, 10, and 20 ng/mL) of recombinant human interleukin-1beta (rhIL-1beta) for 24 hours and then with rhIL-1beta (5 ng/mL) for 3, 6, 9, 12, and 24 hours. Concentration of PGE2 in the media was measured via radioimmunoassay. T...
Viitanen M, Bird J, Maisi P, Smith R, Tulamo RM, May S.As a prerequisite for the identification of navicular disease markers, the concentrations of cartilage oligomeric matrix protein (COMP), total glycosaminoglycans (GAG), hyaluronan, metalloproteinases (MMP) 2 and 9 and total protein were measured in synovial fluid samples obtained from the distal interphalangeal joint (DIP), the metacarpophalangeal joint (MCP) and the navicular bursa of 24 horses. Mean GAG, COMP and total protein levels were significantly higher in the DIP joint and in the navicular bursa compared to the MCP joint. Hyaluronan content was lower. MMP -2 activity was present in al...
Faber NF, Roser JF.Testicular biopsy was performed as a standing procedure on fertile, mature stallions (n=7) under sedation and local anaesthesia. The 'Biopty' instrument was used to collect three samples from the left testis of each stallion. The oestradiol, testosterone and inhibin content in each testis were assessed by validated radioimmunoassay (RIA); protein concentrations were determined and the testes were prepared histologically for haematoxylin and eosin staining. The fertility status of each stallion was assessed before and after testicular biopsy by semen evaluation, changes in total scrotal width, ...
Krumrych W, Wiśniewski E, Danek J.Average values of chosen haematological parameters have been described in a population of 90 clinically normal Polish Primitive Horses. The comparison of the results with values given in literature for horses as a whole and for other breeds enabled us to state that Polish Primitive Horses' blood is characterized by relatively low values of RBC, PCV, segmented neutrophils and monocytes, and high percentage of lymphocytes. It was demonstrated that values of some blood parameters of examined animals are similar to typical results obtained for primitive and cold-blooded horses. Moreover, it was pr...
Bartolomé E, Perdomo-González DI, Ripollés-Lobo M, Valera M.Horses have been valued for their diversity of coat color since prehistoric times. In particular, the pleiotropic effect that coat color genes have on behavior determines the way the horse perceives and reacts to its environment. The primary aim of this study was to evaluate the influence of coat color on basal reactivity assessed with infrared thermography as eye temperature at rest (ETR), determine their relation with the results obtained by these horses in Show Jumping competitions and to estimate the genetic parameters for this variable to test its suitability for genetic selection. A Gene...
Moore RM, Muir WW, Bertone AL, Beard WL, Stromberg PC.Thirty horses were randomly assigned to 1 of 5 groups. All horses were anesthetized and subjected to ventral midline celiotomy, then the large colon was exteriorized and instrumented. Colonic arterial blood flow was reduced to 20% of baseline (BL) and was maintained for 3 hours. Colonic blood flow was then restored, and the colon was reperfused for an additional 3 hours. One of 5 drug solutions was administered via the jugular vein 30 minutes prior to colonic reperfusion: group 1, 0.9% NaCl; group 2, dimethyl sulfoxide: 1 g/kg of body weight; group 3, allopurinol: 25 mg/kg; group 4, 21-aminost...
Martins HS, Martins-Filho OA, Araujo MS, Martins NR, Lagares MA.Frozen equine semen has lower fertility compared to cooled semen. Due to the difficulty to obtain equine oocytes, a heterologous zona pellucida binding assay (ZBA) is an alternative method to predict the fertilizing capability of equine frozen sperm. The rate of capacitated and hyperactivated sperm according to their motility characteristics were analyzed with a Computer Assisted Sperm Analyzer. We believe this report describes for the first time the in vitro hyperactivation induction and the heterologous ZBA to predict the fertilizing ability of frozen equine sperm. Objective: This work aimed...
Watson ED, Buckingham J, Björkstén T, Nikolakopoulos E.The presence of oxytocin in the mare uterus was determined by radioimmunoassay of tissue extracts and uterine lavage fluid, and by immunohistochemistry. Samples were obtained from mares during the oestrous cycle and on day 14 of pregnancy. Immunoreactive oxytocin was detected in extracts of endometrium and myometrium, and in uterine lavage fluid. In tissue sections, the luminal epithelium, the epithelium of the superficial, but not deep, glands, blood vessel walls and myometrium all stained positively for oxytocin, whereas only the luminal epithelium and epithelium of the superficial glands st...
Meghoufel A, Cloutier G, Crevier-Denoix N, de Guise JA.The hypothesis is that an imaging technique based on decompression and segmentation of B-scan images with morphological operators can provide a measurement of the integrity of equine tendons. Methods: Two complementary approaches were used: (i) Simulation of B-scan images to better understand the relationship between image properties and their underlying biological structural contents and (ii) extraction and quantification from B-scan images of tendon structures identified in step (i) to diagnose the status of the superficial digital flexor tendon (SDFT) by using the proposed imaging technique...
Seamans KW, Harms PG, Atkins DT, Fleeger JL.Blood samples were collected from eight mares for ten days before and two days following parturition. Progesterone, 5 alpha-dihydroprogesterone and hydroxy-5 alpha-pregnanones were extracted from serum, separated by Sephadex LH-20 column chromatography and quantitated by radioimmunoassay. Progesterone levels ranged from 8.5 to 4.1 ng/ml among mares with normal parturition and decreased to 1.2 ng/ml after parturition. One mare with a retained placenta maintained the prepartal levels of progesterone into the postpartum period. 5 alpha-dihydroprogesterone increased to a level of 133 ng/ml at 80 h...
Brommer H, Brama PA, Barneveld A, van Weeren PR.The equine metacarpophalangeal (MCP) and metatarsophalangeal (MTP) joints, although having virtually the same geometrical appearance, differ in the prevalence of joint pathologies, such as osteochondral fragmentation, and in biomechanical behaviour. The recently developed cartilage degeneration index (CDI) technique offers a possibility to assess quantitatively differences in cartilage degeneration between these joints and to compare these with known differences in biomechanics and clinical observations. Objective: To compare the topographical distribution of articular cartilage degeneration a...
Jaworska J, Ropka-Molik K, Kowalczyk-Zięba I, Boruszewska D, Wocławek-Potocka I, Siemieniuch M.Physiological parturition is characterized by sterile, inflammatory-like processes. During parturition, the placenta expresses various proinflammatory mediators, such as chemokines and IL-17. Nevertheless, inflammatory processes present in the parturient mare are poorly characterized. The aim of this study was to investigate the expression of selected chemokines and IL-17 in the allantochorion and the endometrium of mares that retained fetal membranes (RFM) and expelled them physiologically. We hypothesized that the expression of these mediators may be altered in the placenta of mares with RFM...
Andersen C, Griffin JF, Jacobsen S, Østergaard S, Walters M, Mori Y, Lindegaard C.Articular cartilage thinning is an important hallmark of osteoarthritis (OA), and ultrasonography (US) is a clinically accessible tool potentially suitable for repeated evaluation. The aim of the present prospective methods comparison study was to validate US as a tool for measuring cartilage thickness in the carpus of the horse. Eight Standardbred trotters underwent US examination with 9 and 15 MHz linear transducers. Six anatomical locations in the radiocarpal joint (RCJ) and middle carpal joint (MCJ) were examined. The same joints were assessed by ultrahigh field (9.4 Tesla) magnetic reson...
Rose RJ, Ilkiw JE, Hodgson D.Thirty-one horses competing in a 100 kilometre endurance ride had electrocardiograms recorded before and after the ride from which the heart score of each horse was calculated. Blood was also taken to determine the packed cell volume (PCV) and total plasma protein (TPP) before the ride, after 60 kilometres (mid ride) and at completion of the ride. Statistical analysis of the heart scores showed that a faster group of horses had significantly higher heart scores than either a slower group or those eliminated due to inadequate recovery of heart rate. No horses developed electrocardiogram abnorma...
Fowden AL, Ellis L, Rossdale PD.Plasma concentrations of insulin and glucose were measured in Pony and Thoroughbred foals at birth and at intervals thereafter for up to 7 days. The plasma concentrations of insulin in foals of both breeds at birth were 11.2 +/- 2.5 microU/ml (N = 6) and 13.5 +/- 1.5 microU/ml (N = 16) respectively. These values were similar to those obtained for foals in utero but were significantly less than those in adult animals. There was little variation in the plasma concentrations of insulin and glucose during the first 2 h of life and both concentrations tended to rise in the next 24-48 h. Although th...
Lavin TE, Nielsen BD, Zingsheim JN, O'Connor-Robison CI, Link JE, Hill GM, Shelton J.An experiment was conducted to study P digestibility in mature horses because of the growing environmental concerns regarding P runoff and previous equine research focused mostly on young and growing animals or used ponies as a model. Phytase supplementation of swine and poultry diets can result in greater phytate-P digestibility, leading to a decreased need for inorganic P supplementation and a decrease in P excreted to the environment; this, however, has not been demonstrated in the horse. Six mature Arabian geldings were fed 6 diets consisting of pelleted concentrate and alfalfa hay. The co...
Seiler GS, Campbell N, Nixon B, Tsuruta JK, Dayton PA, Jennings S, Redding WR, Lustgarten M.Vascular alterations play important roles in many orthopedic diseases such as osteoarthritis, tendonitis, and synovitis in both human and equine athletes. Understanding these alterations could enhance diagnosis, prognosis, and treatment. Contrast-enhanced ultrasound (CEUS) could be a valuable method for evaluation of blood flow and perfusion of these processes in the equine distal limb, however no reports were found describing feasibility or safety of the technique. The goal of this prospective, experimental study was to describe the feasibility and safety of distal limb CEUS in a sample of si...
Wong DM, Hepworth-Warren KL, Sponseller BT, Howard JM, Wang C.OBJECTIVE To assess multiple central venous and arterial blood variables that alone or in conjunction with one another reflect global oxygenation status in healthy neonatal foals. ANIMALS 11 healthy neonatal foals. PROCEDURES Central venous and arterial blood samples were collected from healthy neonatal foals at 12, 24, 36, 48, 72, and 96 hours after birth. Variables measured from central venous and arterial blood samples included oxygen saturation of hemoglobin, partial pressure of oxygen, lactate concentration, partial pressure of carbon dioxide, and pH. Calculated variables included venous-...
Keg PR, van Weeren PR, Back W, Barneveld A.The influence of the force applied and its period of application on the outcome of the flexion test of the distal forelimb was investigated in a group of eight sound horses. The degree of lameness after the flexion test was scored by a standard clinical classification, and by measuring the angle of maximum fetlock extension by means of the infrared light-based MacReflex gait analysis system. There was a good correlation between the clinical score and this electronically recorded kinematic parameter (r = 0.96). Both the force applied and the period of application affected the outcome of the tes...
Gáspárdy A, Rieden S, Simonyi Z, Szemere B, Bodó I.The authors give a short orientation about the age related parameters of the incisors based on the literature. They examined the question of breed differences with respect to the age determination of horses in the different counties, especially in regard to the English Thoroughbred. In this context they present a new parameter as well as a new approach in data-processing. The cup depth and the yearly abrasion showed high breed-specificity. The relative abrasion indicated that the most intensive wear out is typical for the Thoroughbred. The cup depth and the yearly wear out were different accor...
Robertson SA, Steele CJ, Chen CL.The effect of general anaesthesia and arthroscopic surgery on blood glucose and lactate, plasma non-esterified fatty acids, insulin, beta-endorphin and cortisol was investigated in seven horses. Animals were premedicated with xylazine and anaesthesia was induced with guaifenesin and sodium thiamylal and maintained with halothane vaporised in oxygen. Blood samples were collected in the pre-, intra- and post operative period. Induction of anaesthesia was associated with a transient hyperglycaemia and a significant rise in plasma insulin levels. Plasma insulin values fell during surgery but showe...
Allbaugh RA, Keil SM, Ou Z, Bello NM.To assess intraocular pressure (IOP) in conditioned equine athletes and document changes with exercise. A secondary objective was to assess associations between IOP and heart rate, as well as with other subjective physical parameters. Methods: Horses were evaluated during 50 mile endurance ride competitions. Data were collected on 69 horses during five different competitions at three different locations with 59 horses ridden once, nine horses ridden in two competitions, and one horse ridden in three competitions for a total of 80 horse-ride combinations. Methods: Intraocular pressure was measu...
Pringle J, Roberts C, Art T, Lekeux P.This study examined the ability of near infrared spectroscopy (NIRS) to noninvasively determine changes to muscle oxygenation in the resting horse. Five horses had (NIRS) performed over extremity muscle while under general anaesthesia, first with 8 min limb ischaemia, then systemic hypoxaemia for 5 min. A second group of 6 awake horses had NIRS performed over extremity muscle while being administered hypoxic gas (F(I)O2 0.10) for 5 min, and after return to steady state, limb ischaemia was induced for an additional 5 min. In the anaesthetised horses' ischaemia induced marked and significant mus...
Pagger H, Schmidburg I, Peham C, Licka T.This aim of this study was to test the hypothesis that the stiffness of the equine cervical spine depends on the direction of force applied and on spinal position. Muscles and nuchal ligament were removed from the cervical spines of 17 horses that were without a history of cervical or neurological disease. The cervical spines were then flexed/extended dorsoventrally (with the spine straight and with the occiput rotated 30° against T1) and laterolaterally. Mean dorsoventral stiffness was 297 N/m (± 135) in flexion, 1347 N/m (± 2083) in extension, 421 N/m (± 164) in lateral flexion, 279 N/m ...
Venugopal CS, Moore RM, Holmes EP, Koch CE, Seahorn TL, Beadle RE.To compare responses of bronchial rings obtained from healthy horses and horses affected with summer pasture-associated obstructive pulmonary disease (SPAOPD) to selected mediators of airway hyperreactivity in vitro. Methods: Bronchial rings from 6 healthy horses and 6 horses affected with SPAOPD. Methods: Bronchial rings obtained from each group of horses were mounted in organ baths and attached to force transducers interfaced with a polygraph. After applying 2g of tension, each ring was allowed to equilibrate for 45 minutes in Tyrode's solution at 37 C. Cumulative concentration-response rela...
Fanelli D, Tesi M, Rota A, Beltramo M, Conte G, Giorgi M, Barsotti G, Camillo F, Panzani D.Human Chorionic Gonadotropin (hCG) and Gonadotropin Releasing Hormone agonists (GnRHa) are routinely used to induce ovulation in mares. However, GnRHa efficacy in transitional mares has been suggested to be low. Objective: The aims of this study were as follows: (a) to compare the efficacy of hCG and GnRHa in inducing the first ovulation of the breeding season and (b) to evaluate the correlation between ovulatory response, uterine oedema and teasing score at the time of treatment during the early or late transitional phase. Methods: Randomised controlled superiority trial. Methods: Mares in wi...
Greube A, Müller K, Töpfer-Petersen E, Herrmann A, Müller P.Seminal plasma of mammalians contains, among others, proteins that are characterized by the fibronectin (Fn) type II module. Our knowledge about the structure and the physiological function of seminal Fn type II proteins mainly originates from studies on PDC-109, the bovine representative of this protein family. The present work focuses on the equine protein SP-1/2 (also named HSP-1/2) with particular emphasis on its interaction with lipid membranes by employing the intrinsic protein fluorescence and a number of spin-labeled and fluorescent lipid analogues. The results indicate that the intera...
