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Topic:Veterinary Research

Veterinary research in horses encompasses the study of diseases, health management, and medical treatments specific to equine species. This field investigates various aspects of horse health, including infectious diseases, metabolic disorders, and musculoskeletal conditions. Researchers focus on understanding the pathophysiology of equine ailments, developing diagnostic tools, and evaluating therapeutic interventions. The study of horse health also involves examining preventive measures such as vaccination protocols and nutritional management to promote overall well-being. This page collects peer-reviewed research studies and scholarly articles that explore the diverse areas of veterinary science related to horses, providing insights into disease mechanisms, treatment strategies, and advancements in equine healthcare.
Artifactual nonlinearity due to wear grooves and friction in four-point bending experiments of cortical bone.
Journal of biomechanics    February 1, 1997   Volume 30, Issue 2 185-188 doi: 10.1016/s0021-9290(96)00124-8
Griffin LV, Gibeling JC, Gibson VA, Martin RB, Stover SM.Experiments and analyses were performed to determine the cause of a nonlinear force-deflection response observed in four-point flexural fatigue of beams of cortical bone machined from the mid-diaphysis of the equine third metacarpus. Observable grooves which formed on the beam surface at supports and load noses were found to be the primary cause of the nonlinearity. An additional geometric nonlinearity at large deflections revealed by finite element modeling may be minimized by using the smallest diameter supports and load noses recommended in ASTM 790. However, frictional constraint of the be...
Subjecting horse spermatozoa to hypoosmotic incubation: effects of ouabain.
Theriogenology    February 1, 1997   Volume 47, Issue 3 765-784 doi: 10.1016/s0093-691x(97)00033-2
de la Cueva FI, Rigau T, Bonet S, Miró J, Briz M, Rodríguez-Gil JE.Although hypoosmotic tests are widely used to assess spermatozoal quality in different species, they have not been used extensively in the stallion. Moreover, the role of the Na (+)K (+), ouabain sensitive-ATP-ase in the response of equine sperm to hypoosmotic shock is not well understood. This study tests two hypotheses: 1) that equine spermatozoa will respond to a hypoosmotic medium by swelling of the tail, and 2) that addition of ouabain will increase the percentage of swollen sperm tails. Ejaculates from 3 stallions were collected with an artificial vagina and diluted in Kenney's medium (T...
Residual strength of equine bone is not reduced by intense fatigue loading: implications for stress fracture.
Journal of biomechanics    February 1, 1997   Volume 30, Issue 2 109-114 doi: 10.1016/s0021-9290(96)00113-3
Martin RB, Gibson VA, Stover SM, Gibeling JC, Griffin LV.Fatigue or stress fractures are an important clinical problem in humans as well as racehorses. An important question in this context is, when a bone experiences fatigue damage during extreme use, how much is it weakened compared to its original state? Since there are very limited data on this question and stress fractures are common in racehorses, we sought to determine the effect of fatigue loading on the monotonic strength of equine cortical bone. Beams were machined from the dorsal, medial and lateral cortices of the third metacarpal bones of six thoroughbred racehorses. Beams from left and...
Pasteurella caballi infection following a horse bite.
Zentralblatt fur Bakteriologie : international journal of medical microbiology    February 1, 1997   Volume 285, Issue 3 440-444 doi: 10.1016/s0934-8840(97)80010-2
Escande F, Vallee E, Aubart F.The isolation of Pasteurella caballi from an horse-bite wound in a 56-year-old man is reported. Biochemical characteristics are described and compared with the other species representing the genus Pasteurella. This strain probably represents the first human isolate of P. caballi in France.
Removal of a nasal polyp in a standing horse.
The Canadian veterinary journal = La revue veterinaire canadienne    February 1, 1997   Volume 38, Issue 2 108-109 
Watt BC, Beck BE.Diagnosis and removal of a nasal polyp in a horse using standing chemical restraint and readily available equipment are described. Histopathology of the polyp and differential diagnoses are discussed.
Determination of daily sperm production in stallion testes by enumeration of germ cells in homogenates.
Theriogenology    February 1, 1997   Volume 47, Issue 3 655-664 doi: 10.1016/s0093-691x(97)00024-1
Blanchard TL, Johnson L.Thirty adult stallion testes were selected with high (n = 15) and low (n = 15) Daily Sperm Production (DSP)/testis. Parenchymal samples were prepared for morphometric analysis, and the numbers of germ cells and Sertoli cells were determined. Testicular samples were homogenized, and germ cells and Sertoli cells were enumerated using phase contrast microscopy. Numbers of germ cells and Sertoli cells and potential DSP during spermatogenesis were determined. Significant correlations existed between morphometric and homogenate determinations of number per testis of preleptotene, leptotene plus zygo...
Serologic markers in early stages of African horse sickness virus infection.
Journal of clinical microbiology    February 1, 1997   Volume 35, Issue 2 531-535 doi: 10.1128/jcm.35.2.531-535.1997
Martínez-Torrecuadrada JL, Díaz-Laviada M, Roy P, Sánchez C, Vela C, Sánchez-Vizcaíno JM, Casal JI.Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependen...
Pharmacokinetics of intravenous and oral prethcamide in horses.
Journal of pharmaceutical and biomedical analysis    February 1, 1997   Volume 15, Issue 5 639-651 doi: 10.1016/s0731-7085(96)01885-7
Sams RA, Gerken DF, Ashcraft SM.The respiratory stimulant prethcamide is a mixture of equal parts of crotethamide and cropropamide. A specific and sensitive gas chromatographic method for the determination of crotethamide and cropropamide in horse plasma and urine is described. Both components of prethcamide were extracted from plasma and urine into dichloromethane. The extracts were analyzed by capillary gas chromatography with thermionic detection in the nitrogen-specific detection mode. The lower limits of quantitation were 4.0 ng ml-1 of plasma and 10.0 ng ml-1 of urine. Calibration curves were linear from 2.0-100 ng ml-...
Pregnancy-associated changes in material properties of the third metacarpal cortical bone in mares.
American journal of veterinary research    February 1, 1997   Volume 58, Issue 2 182-187 
Hawkins DL, Stover SM.To investigate the effect of late gestation, age, and parity on material properties of third metacarpal (MCIII) cortical bone in mares. Methods: 8 healthy mares (treatment group) that died or were euthanatized within 24 hours after parturition because of foaling complications and 6 age-matched, healthy, nonpregnant mares (control group). Methods: After random assignment for mechanical testing and microradiography, the dorsal half of transverse mid-diaphyseal sections of each MCIII bone was divided into lateral, dorsal, and medial regions. Cylinders of bone from each of the 3 regions were teste...
Experimental induction of equine protozoal myeloencephalitis in horses using Sarcocystis sp. sporocysts from the opossum (Didelphis virginiana).
Veterinary parasitology    February 1, 1997   Volume 68, Issue 3 199-213 doi: 10.1016/s0304-4017(96)01112-0
Fenger CK, Granstrom DE, Gajadhar AA, Williams NM, McCrillis SA, Stamper S, Langemeier JL, Dubey JP.Sarcocystis sp. sporocysts isolated from eight feral opossums (Didelphis virginiana) were pooled and fed to 18 commercially reared budgerigars (Melopsittacus undulatus), 14 wild-caught sparrows (Passer domesticus), one wild-caught slate-colored Junco (Junco hyemalis) and five weanling horses (Equus caballus). All budgerigars died within 5 weeks post inoculation (wpi). Histologic examination revealed meronts within the pulmonary epithelia and typical Sarcocystis falcatula sarcocysts developing in the leg muscles. Sparrows were euthanized 13 and 17 wpi and their carcasses were fed to four labora...
Antibiotic prophylaxis of lower respiratory tract contamination in horses confined with head elevation for 24 or 48 hours.
Australian veterinary journal    February 1, 1997   Volume 75, Issue 2 126-131 doi: 10.1111/j.1751-0813.1997.tb14172.x
Raidal SL, Taplin RH, Bailey GD, Love DN.To evaluate the administration of procaine penicillin prior to or during confinement with head elevation as a means of reducing the associated accumulation of inflammatory lower respiratory tract secretions and increased numbers of bacteria within the lower respiratory tract of confined horses. Methods: Two experiments were conducted to evaluate the efficacy of different dose rates and dosing frequencies. In experiment A a single low dose (15,000 IU/kg) of procaine penicillin was administered to four horses immediately prior to confinement with head elevation for 48 hours. The systemic leucocy...
Erythrocyte glutathione-S-transferase activity in animal species.
Veterinary and human toxicology    February 1, 1997   Volume 39, Issue 1 9-11 
Vodela JK, Dalvi RR.This study was conducted to determine and compare the activities of glutathione-S-transferase (GST) in red blood cells of cattle, horses, pigs, goats, dogs, rabbits, rats and mice. The highest GST activity was found in mouse red blood cells followed by that of rats, dogs, cattle, pigs, goats and horses with the lowest activity in rabbits. There were significant differences between the GST activities from these various species. The species differences in GST activities correlate with the reported variable responses of the different species to different toxicants since erythrocyte GST plays a si...
Serum thyroid hormone concentrations in New Zealand horses.
New Zealand veterinary journal    February 1, 1997   Volume 45, Issue 1 11-14 doi: 10.1080/00480169.1997.35981
Thompson JC, Ellison RS, Kirk J.Total thyroxine and total tri-iodothyronine concentrations were measured in the sera from 125 horses of mixed age, breed and sex, and varied clinical histories. While low serum thyroxine concentrations were detected in 35 horses, the majority of those horses had serum thyroxine values within the reference range when retested. Only one horse had a mildly decreased serum tri-iodothyronine concentration. Those horses in which the serum thyroxine concentration was low when retested had a normal thyrotropin releasing hormone stimulation test. Hypothyroidism was not diagnosed in any horses in this s...
Case report: field-acquired subclinical Babesia equi infection confirmed by in vitro culture.
Journal of clinical microbiology    February 1, 1997   Volume 35, Issue 2 474-476 doi: 10.1128/jcm.35.2.474-476.1997
Holman PJ, Hietala SK, Kayashima LR, Olson D, Waghela SD, Wagner GG.A horse with no prior clinical history of equine piroplasmosis tested negative for Babesia caballi and Babesia equi in the complement fixation test before importation into the United States from France. After 5 years in residence in the United States, the animal tested serologically positive for B. equi by the complement fixation test, the immunofluorescent antibody test, and Western blot analysis. The carrier status of the horse was confirmed by culture of B. equi parasites. In vitro culture offers an efficient and comparatively inexpensive method to determine the carrier status of horses sus...
The Michigan equine monitoring system. I. Design, implementation and population estimates.
Preventive veterinary medicine    February 1, 1997   Volume 29, Issue 4 263-275 doi: 10.1016/s0167-5877(96)01079-3
Kaneene JB, Saffell M, Fedewa DJ, Gallagher K, Chaddock HM.The Michigan equine monitoring system (MEMS) was designed and implemented in the State of Michigan, starting in 1991. The program was designed systematically to track the State's equine population, its health, and its economic implications to the equine industry. The MEMS was designed as a two-phase program. Phase I (the population and economic survey; the subject of this paper) was designed to provide new and statistically valid information describing the size, composition, location and economic characteristics of the Michigan equine industry. A standardized questionnaire was used to collect ...
Legal rights of veterinarians under veterinary Good Samaritan statutes and equine liability statutes.
Journal of the American Veterinary Medical Association    January 15, 1997   Volume 210, Issue 2 190-194 
Centner TJ.No abstract available
High-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres.
Journal of chromatography. B, Biomedical sciences and applications    January 10, 1997   Volume 688, Issue 1 47-55 doi: 10.1016/s0378-4347(97)88054-1
Dunnett M, Harris RC.The combined solid-phase extraction (Isolute PRS columns) and reversed-phase gradient HPLC method presented provides a sensitive, reproducible and selective quantification of carnosine, balenine, homocarnosine, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres. Recoveries were 91-115%. Lower limits of detection were 0.005-0.010 mmol kg-1 dry muscle. The compounds were isolated from other physiological amino acids and small peptides and resolved within a single chromatographic run of 55 min. Concentrations of these compounds in equine myo...
Gazelle herpesvirus 1: a new neurotropic herpesvirus immunologically related to equine herpesvirus 1.
Virology    January 6, 1997   Volume 227, Issue 1 34-44 doi: 10.1006/viro.1996.8296
Fukushi H, Tomita T, Taniguchi A, Ochiai Y, Kirisawa R, Matsumura T, Yanai T, Masegi T, Yamaguchi T, Hirai K.A herpesvirus was isolated from Thomson's gazelle (Gazella thomsoni) kept at a zoological garden in Japan during an outbreak of epizootic acute encephalitis. The virus, gazelle herpesvirus 1 (GHV-1), was serologically related to equine herpesvirus 1 (EHV-1). However, DNA fingerprints of GHV-1 were different from those of EHV-1 and other equine herpesviruses. Southern hybridization with probes of cloned BamHI fragments derived from UL and US segments of EHV-1 revealed differences in the DNA restriction profiles throughout the entire genome. Nucleotide sequences were determined for a conserved r...
Dose-response of X-irradiated human and equine lymphocytes.
Mutation research    January 3, 1997   Volume 373, Issue 1 9-16 doi: 10.1016/s0027-5107(96)00178-9
Catena C, Asprea L, Carta S, Tortora G, Conti D, Parasacchi P, Righi E.We have investigated and compared DNA damage and cell killing induced in human and equine lymphocytes after in vitro X-irradiation. Our data show that the cytogenetic and the lethality effects are both greater in equine lymphocytes, but that the difference is wider for lethality. The ratios between doses inducing the same effect are 1.3, 1.7 and 9.4 for the number of binucleated cells with micronuclei, micronucleus frequency in binucleated cells and DNA synthesis inhibition, respectively. The very different radiosensitivity observed for the two mammalian species encourages us to use their lymp...
Chylothorax and meconium impaction in a neonatal colt.
Equine veterinary journal    January 1, 1997   Volume 29, Issue 1 77-79 doi: 10.1111/j.2042-3306.1997.tb01643.x
Scarratt WK, Wallace MA, Pleasant RS, Sysel AM, Feldman B, Jones JC.No abstract available
Relationship between endometrial oxytocin receptors and oxytocin-induced prostaglandin F2 alpha release during the oestrous cycle and early pregnancy in pony mares.
Journal of reproduction and fertility    January 1, 1997   Volume 109, Issue 1 137-144 doi: 10.1530/jrf.0.1090137
Sharp DC, Thatcher MJ, Salute ME, Fuchs AR.The effect of transcervical endometrial biopsy on the concentrations of plasma immunoreactive oxytocin and 15-keto-13,14-dihydro-prostaglandin F2 alpha (PGFM) was studied in 18 pony mares on days 8, 12 and 14 after ovulation, days 12 and 14 of early pregnancy and at oestrus. Five biopsy specimens were taken within 15 min and consecutive specimens from each mare were pooled two (A) and three (B) together for measurement of the number of oxytocin receptors. Blood samples were collected at intervals of 5 min for 15 min beginning just before the initial biopsy. Biopsy procedure elicited prompt oxy...
Characterization of horse (Equus caballus) immunoglobulin mu chain-encoding genes.
Immunogenetics    January 1, 1997   Volume 45, Issue 6 386-393 doi: 10.1007/s002510050220
Schrenzel MD, King DP, McKnight ML, Ferrick DA.Horse (Equus caballus) immunoglobulin mu chain-encoding (IgM) variable, joining, and constant gene segments were cloned and characterized. Nucleotide sequence analyses of 15 cDNA clones from a mesenteric lymph node library identified 7 unique variable gene segments, 5 separate joining segments, and a single constant region. Based on comparison with human sequences, horse variable segments could be grouped into either family 1 of immunoglobulin (Ig) clan I or family 4 of Ig clan II subclan IV. All horse sequences had a relatively conserved 16 base pair (bp) segment in framework 3 which was reco...
Radiation hazards from horses undergoing scintigraphy using technetium-99m.
Equine veterinary journal    January 1, 1997   Volume 29, Issue 1 26-30 doi: 10.1111/j.2042-3306.1997.tb01632.x
Whitelock RG.This paper quantifies the extent of the radiation hazard to personnel from horses undergoing scintigraphy using technetium99m methylene diphosphonate (99Tcm-MDP). From the data produced it is possible to derive safe working protocols which are comfortably within the legislated limits for whole body doses as set out in the Ionising Radiations Regulations 1985. Measurements were made of the surface and environmental activities which result from individuals undergoing scintigraphic evaluation and also from urine contaminated bedding. The use of both high and low activities in the assessment of th...
[Animal systemic iron sources utilized in vitro by staphylococci].
Medycyna doswiadczalna i mikrobiologia    January 1, 1997   Volume 49, Issue 1-2 45-53 
Lisiecki P, Sobiś-Glinkowska M, Mikucki J.Under iron-restricted conditions staphylococcal strains could utilize in vitro several animals body iron sources in form of bovine haemoglobin, hemin, lactoferrin and transferrin, ovotransferrin, horse myoglobin ferritin and cytochrome C. Spectrum of utilized iron sources was not dependent on species affiliation and kind of siderophores system. Strains isolated from clinical materials utilized largest spectrum of animal iron body sources.
Comparison of racing performance before and after treatment of incomplete, midsagittal fractures of the proximal phalanx in standardbreds: 49 cases (1986-1992).
Journal of the American Veterinary Medical Association    January 1, 1997   Volume 210, Issue 1 82-86 
Tetens J, Ross MW, Lloyd JW.To assess the effect of incomplete, midsagittal fractures of the proximal phalanx (P1) on racing performance in Standardbreds. Methods: Retrospective cohort study. Methods: 49 Standardbred horses admitted to the George D. Widener Hospital for Large Animals between July 1986 and December 1992 with a definitive radiographic diagnosis of an incomplete, midsagittal fracture of P1 and a known method of treatment. Methods: Performance index and racing time were compared before and after diagnosis and treatment of fracture, using ANOVA that controlled for the effects of horse, gender, age, track leng...
Atrial natriuretic peptide and C-type natriuretic peptide do not acutely inhibit the release of adrenocorticotropin from equine pituitary cells in vitro.
Neuroendocrinology    January 1, 1997   Volume 65, Issue 1 64-69 doi: 10.1159/000127165
Mulligan RS, Livesey JH, Evans MJ, Ellis MJ, Donald RA.It has been suggested that atrial natriuretic peptide (ANP) is the long-sought inhibitor of corticotropin (ACTH) secretion, but the evidence is conflicting. We have examined the effect of ANP and C-type natriuretic peptide (CNP) on the secretion of ACTH by perifused equine pituitary cells in an in vitro milieu intended to mimic the in vivo milieu in the horse. Corticotropin-releasing hormone (20 pM) and cortisol (0 or 100 nM) were perifused continuously and 7 pulses of arginine vasopressin (AVP; 10 nM) applied for 5 min at 30-min intervals. ANP (1 nM) or CNP (1 nM) were perifused continuously ...
Identification, cloning and sequence analysis of the equine adenovirus 1 hexon gene.
Archives of virology    January 1, 1997   Volume 142, Issue 6 1193-1212 doi: 10.1007/s007050050152
Reubel GH, Studdert MJ.Based on sequence homology with human adenovirus 2 (HAdV2), the hexon gene of equine adenovirus 1 (EAdV1) was identified. HindIII restriction fragments containing the hexon and other viral genes were cloned into the plasmids pUC19 and pBlueScript SK(-) and sequenced. The nucleotide sequence of the hexon gene was completely determined and partial sequence data were obtained for seven other EAdV1 genes. Amino acid (aa) sequence comparison with published adenovirus (AdV) proteins identified the genes for the IIIa, penton, pVII, PVI, 23K proteinase, DNA binding and 100K proteins. The eight EAdV1 g...
Thelazia lacrymalis (Nematoda, Spirurida, Thelaziidae): report in a horse in Germany and contribution to the morphology of adult worms.
Parasitology research    January 1, 1997   Volume 83, Issue 6 627-631 doi: 10.1007/s004360050309
Beelitz P, Dongus H, Schöl H, Gerhards H, Gothe R.No abstract available
[Poisoning of horses by the bark of the false acacia (Robinia pseudoacacia)].
Schweizer Archiv fur Tierheilkunde    January 1, 1997   Volume 139, Issue 8 363-366 
Landolt G, Feige K, Schöberl M.The present study describes the poisoning due to Robinia pseudoacacia in two horses. One of the horses showed mainly intestinal symptoms such as decreased intestinal motility and obstipation of the pelvic flexure. In the second animal central nervous symptoms were predominant. It showed intermittent phases of somnolence alternating with phases of excitation and head pressing. In addition mydriasis and a lacking menace response could be observed. Sensation to the head and the spinal reflexes were reduced. Symptomatic treatment was initiated in both animals and recovery occurred within 2 days.
Adaptation of equine herpesvirus 1 to unnatural host led to mutation of the gC resulting in increased susceptibility of the virus to heparin.
Archives of virology    January 1, 1997   Volume 142, Issue 9 1849-1856 doi: 10.1007/s007050050202
Sugahara Y, Matsumura T, Kono Y, Honda E, Kida H, Okazaki K.Heparin extensively inhibited infection of MDBK cells by equine herpesvirus 1 (EHV-1) strains adapted to bovine cells or hamsters, while the reagent merely reduced infectivity of strains passaged only in equine cells. The gC of two strains adapted to non-equine cells seemed to have higher affinity for heparin, although the reagent bound to both the gC and gB of all strains tested. Amino acid substitutions of the gC of the EHV-1 strains adapted to non-equine cells converged on the hydrophilic regions, amino acid residues 92 to 175, resulting in the glycoprotein becoming more cationic. These res...