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Home / Equine Research Bank / Mamm Genome / A whole-genome scan for recurrent airway obstruction in Warm...
Mammalian genome : official journal of the International Mammalian Genome Society2009; 20(8); 504-515; doi: 10.1007/s00335-009-9214-5

A whole-genome scan for recurrent airway obstruction in Warmblood sport horses indicates two positional candidate regions.

Abstract: Recurrent airway obstruction (RAO), or heaves, is a naturally occurring asthma-like disease that is related to sensitisation and exposure to mouldy hay and has a familial basis with a complex mode of inheritance. A genome-wide scanning approach using two half-sibling families was taken in order to locate the chromosome regions that contribute to the inherited component of this condition in these families. Initially, a panel of 250 microsatellite markers, which were chosen as a well-spaced, polymorphic selection covering the 31 equine autosomes, was used to genotype the two half-sibling families, which comprised in total 239 Warmblood horses. Subsequently, supplementary markers were added for a total of 315 genotyped markers. Each half-sibling family is focused around a severely RAO-affected stallion, and the phenotype of each individual was assessed for RAO and related signs, namely, breathing effort at rest, breathing effort at work, coughing, and nasal discharge, using an owner-based questionnaire. Analysis using a regression method for half-sibling family structures was performed using RAO and each of the composite clinical signs separately; two chromosome regions (on ECA13 and ECA15) showed a genome-wide significant association with RAO at P < 0.05. An additional 11 chromosome regions showed a more modest association. This is the first publication that describes the mapping of genetic loci involved in RAO. Several candidate genes are located in these regions, a number of which are interleukins. These are important signalling molecules that are intricately involved in the control of the immune response and are therefore good positional candidates.
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Publication Date: 2009-09-18 PubMed ID: 19760324DOI: 10.1007/s00335-009-9214-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research in this article investigates the genetic origins of recurrent airway obstruction (RAO) in Warmblood sport horses, identifying two significant chromosome regions potentially linked to the disease.

Study Background and Objective

  • This research focuses on Recurrent Airway Obstruction (RAO), a health condition in Warmblood sport horses similar to asthma in humans. This disease is related to exposure to mouldy hay and has a genetic basis with a complex inheritance pattern.
  • The study aims to identify the chromosomal regions associated with the inherited component of RAO using a genome-wide scanning technique.

Research Methodology

  • The researchers used two half-sibling Warmblood families for their study, centering on a severely RAO-impacted stallion in each family.
  • Initially, the researchers genotyped these families using a panel of 250 microsatellite markers. These markers provided a wide and varied coverage of the 31 equine autosomes.
  • The study later added extra markers, leading to a total of 315 genotyped markers.
  • The researchers assessed the severity of RAO symptoms in each individual horse through an owner-based questionnaire. Symptoms considered included breathing difficulty at rest or during work, coughing, and nasal discharge.
  • A regression method was employed to analyze the RAO symptoms and the genotypic structures of the half-sibling families.

Results

  • The results highlight two chromosomal regions, on ECA13 and ECA15, significantly associated with RAO.
  • 11 other chromosomal regions showed a possible but less pronounced relationship.
  • This study is the first to scientifically map the genetic loci involved in RAO.

Implications and Conclusions

  • The identified regions contain several candidate genes for RAO, including a number of interleukins, which are vital to controlling immune responses.
  • The findings can help understand the genetic basis of RAO and pave the way for further studies and potential treatments.

Cite This Article

APA
Swinburne JE, Bogle H, Klukowska-Rötzler J, Drögemüller M, Leeb T, Temperton E, Dolf G, Gerber V. (2009). A whole-genome scan for recurrent airway obstruction in Warmblood sport horses indicates two positional candidate regions. Mamm Genome, 20(8), 504-515. https://doi.org/10.1007/s00335-009-9214-5

Publication

Mammalian genome : official journal of the International Mammalian Genome Society
ISSN: 1432-1777
NlmUniqueID: 9100916
Country: United States
Language: English
Volume: 20
Issue: 8
Pages: 504-515

Researcher Affiliations

Swinburne, June E
  • Animal Health Trust, Newmarket, Suffolk CB8 7UU, UK. june.swinburne@aht.org.uk
Bogle, Helen
    Klukowska-Rötzler, Jolanta
      Drögemüller, Michaela
        Leeb, Tosso
          Temperton, Elizabeth
            Dolf, Gaudenz
              Gerber, Vincent

                MeSH Terms

                • Airway Obstruction / genetics
                • Airway Obstruction / veterinary
                • Animals
                • Chromosome Mapping
                • Female
                • Genetic Linkage
                • Genome-Wide Association Study / veterinary
                • Horse Diseases / genetics
                • Horses
                • Male
                • Pedigree
                • Quantitative Trait Loci

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                This article has been cited 15 times.
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