FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
The Veterinary clinics of North America. Equine practice2016; 32(3); 521-530; doi: 10.1016/j.cveq.2016.08.003

Advances in Stallion Semen Cryopreservation.

Abstract: The use of stallion frozen semen minimizes the spread of disease, eliminates geographic barriers, and preserves the genetic material of the animal for an unlimited time. Significant progress on the frozen thawed stallion semen process and consequently fertility has been achieved over the last decade. These improvements not only increased fertility rates but also allowed cryopreservation of semen from "poor freezers." This article reviews traditional steps and new strategies for stallion semen handling and processing that are performed to overcome the deleterious effects of semen preservation and consequently improve frozen semen quality and fertility.
Copyright © 2016 Elsevier Inc. All rights reserved.
Get Full Text
Publication Date: 2016-10-08 PubMed ID: 27726989DOI: 10.1016/j.cveq.2016.08.003Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Review

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses the technological improvements made over the past decade in the cryopreservation of stallion semen, which has led to improved fertility rates and enabled the successful freezing of semen from “poor freezers”.

Advances in Stallion Semen Cryopreservation

  • The research article primarily focuses on recent advancements in the cryopreservation of stallion semen. Cryopreservation is a process that allows sperm to be frozen and stored for later use. This technique is beneficial as it minimizes the spread of disease, eradicates geographic barriers, and allows the preservation of an animal’s genetic material for an unlimited time period.
  • Significant improvements in this process over the past decade have resulted in increased fertility rates. Moreover, these advancements have also enabled the preservation of semen from stallions that were previously categorized as “poor freezers” – referring to animals whose semen did not freeze well in the past.

Traditional Steps and New Strategies

  • The article critically reviews the traditional steps involved in the handling and processing of stallion semen. While the traditional methods have their own advantages, the development and incorporation of new strategies were necessary to further enhance the efficiency of semen preservation.
  • The deleterious effects or potential damage that could occur to semen during preservation are key concerns in this process. The researchers highlight how newly devised strategies and advancements have helped to overcome these challenges, thereby improving both the quality of frozen semen and fertility rates.

Implications of the Study

  • The study is of significant importance in the field of animal breeding and genetics. By outlining new techniques in the cryopreservation of stallion semen, it champions a more effective method for preserving stallion genetic material.
  • Moreover, it implies that these improvements can lead to a minimization of disease transmission among horses and the eradication of geographic constraints. This means that stallion semen can be transported and used almost anywhere in the world without losing the quality and potential effectiveness of the semen, opening a gateway to global trading and conservation efforts.

Cite This Article

APA
Alvarenga MA, Papa FO, Ramires Neto C. (2016). Advances in Stallion Semen Cryopreservation. Vet Clin North Am Equine Pract, 32(3), 521-530. https://doi.org/10.1016/j.cveq.2016.08.003

Publication

The Veterinary clinics of North America. Equine practice
ISSN: 1558-4224
NlmUniqueID: 8511904
Country: United States
Language: English
Volume: 32
Issue: 3
Pages: 521-530

Researcher Affiliations

Alvarenga, Marco Antonio
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University-UNESP, Botucatu, Brazil. Electronic address: malvarenga.fmvz.unesp.br@gmail.com.
Papa, Frederico Ozanam
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University-UNESP, Botucatu, Brazil.
Ramires Neto, Carlos
  • Department of Animal Reproduction and Veterinary Radiology, São Paulo State University-UNESP, Botucatu, Brazil.

MeSH Terms

  • Animals
  • Cryopreservation / veterinary
  • Horses
  • Male
  • Semen / physiology
  • Semen Preservation / veterinary

Citations

This article has been cited 15 times.
  1. Rich J, Orbach DN. Artificial vagina conformation and composition for semen collection. Reprod Fertil 2025 Oct 1;6(4).
    doi: 10.1530/RAF-25-0061pubmed: 41247060google scholar: lookup
  2. Brito LFC, Linardi RL, Rosales LAS, Balamurugan NS, Hernández-Avilés C, Ramírez-Agámez L. Evaluation of a Chemically Defined, Long-Term Extender for Liquid Storage of Stallion Semen. Reprod Domest Anim 2025 Sep;60(9):e70126.
    doi: 10.1111/rda.70126pubmed: 41002042google scholar: lookup
  3. Bugno-Poniewierska M, Bielecka M, Pietras N, Kij-Mitka B, Podstawski Z, Długosz B. Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion Spermatozoa. Animals (Basel) 2025 Jun 28;15(13).
    doi: 10.3390/ani15131915pubmed: 40646814google scholar: lookup
  4. Al-Kass Z, Morrell JM, Ntallaris T. Effect of Centrifugation of Stallion Semen Through a Low Density Colloid Prior to Freezing on Sperm Cryosurvival. Animals (Basel) 2025 Jun 25;15(13).
    doi: 10.3390/ani15131881pubmed: 40646780google scholar: lookup
  5. Catalán J, Yánez-Ortiz I, Torres-Garrido M, Ribas-Maynou J, Llavanera M, Barranco I, Yeste M, Miró J. Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen-Thawed Horse Sperm. Antioxidants (Basel) 2024 Mar 6;13(3).
    doi: 10.3390/antiox13030322pubmed: 38539855google scholar: lookup
  6. Shen W, Fu Y, Bai H, Zhang Z, Cao Z, Liu Z, Yang C, Sun S, Wang L, Ren C, Ling Y, Zhang Z, Cao H. Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature. Anim Biosci 2024 Apr;37(4):640-654.
    doi: 10.5713/ab.23.0329pubmed: 38271968google scholar: lookup
  7. Díaz Rojas E, Carrillo Moreno DI, Contreras Villarreal V, Arellano Rodríguez F, Alvarado Espino AS, Ángel García O. Effect of nutraceutical supplementation on semen quality in stallions. Vet Med Sci 2023 Nov;9(6):2600-2605.
    doi: 10.1002/vms3.1289pubmed: 37817488google scholar: lookup
  8. Podico G, Spencer KM, Magalhaes HB, Canisso IF. Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing. Vet Sci 2023 Feb 21;10(3).
    doi: 10.3390/vetsci10030173pubmed: 36977212google scholar: lookup
  9. Catalán J, Yánez-Ortiz I, Tvarijonaviciute A, González-Aróstegui LG, Rubio CP, Barranco I, Yeste M, Miró J. Seminal Plasma Antioxidants Are Related to Sperm Cryotolerance in the Horse. Antioxidants (Basel) 2022 Jun 28;11(7).
    doi: 10.3390/antiox11071279pubmed: 35883774google scholar: lookup
  10. Al-Khaldi K, Yimer N, Sadiq MB, Firdaus Jesse Bin Abdullah F, Salam Babji A, Al-Bulushi S. Edible bird's nest supplementation in chilled and cryopreserved Arabian stallion semen. Saudi J Biol Sci 2022 Mar;29(3):1577-1584.
    doi: 10.1016/j.sjbs.2021.11.009pubmed: 35280569google scholar: lookup
  11. Bazzano M, Laus F, Spaterna A, Marchegiani A. Use of nutraceuticals in the stallion: Effects on semen quality and preservation. Reprod Domest Anim 2021 Jul;56(7):951-957.
    doi: 10.1111/rda.13934pubmed: 33772909google scholar: lookup
  12. Gimeno BF, Bariani MV, Laiz-Quiroga L, Martínez-León E, Von-Meyeren M, Rey O, Mutto AÁ, Osycka-Salut CE. Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen-Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status. Animals (Basel) 2021 Jan 3;11(1).
    doi: 10.3390/ani11010074pubmed: 33401609google scholar: lookup
  13. Papas M, Catalán J, Recuero S, Morrell JM, Yeste M, Miró J. Single Layer Centrifugation Improves the Quality of Fresh Donkey Semen and Modifies the Sperm Ability to Interact with Polymorphonuclear Neutrophils. Animals (Basel) 2020 Nov 16;10(11).
    doi: 10.3390/ani10112128pubmed: 33207812google scholar: lookup
  14. Papas M, Catalán J, Fernandez-Fuertes B, Arroyo L, Bassols A, Miró J, Yeste M. Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance. Antioxidants (Basel) 2019 Nov 9;8(11).
    doi: 10.3390/antiox8110539pubmed: 31717586google scholar: lookup
  15. Perez-Patiño C, Barranco I, Li J, Padilla L, Martinez EA, Rodriguez-Martinez H, Roca J, Parrilla I. Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa. Int J Mol Sci 2019 Apr 11;20(7).
    doi: 10.3390/ijms20071791pubmed: 30978930google scholar: lookup
Subscribe to our newsletter
Join 99,780 horse owners like you who receive our email updates on horse health and nutrition.