An Investigation of Equine Mesenchymal Stem Cell Characteristics from Different Harvest Sites: More Similar Than Not.
Abstract: Diseases of the musculoskeletal system are a major cause of loss of use and retirement in sport horses. The use of bone marrow-derived mesenchymal stem cells (BMDMSCs) for healing of traumatized tissue has gained substantial favor in clinical settings and can assist healing and tissue regeneration in orthopedic injuries. There are two common sites of harvest of BMDMSCs, the sternum and the ilium. Our objective was to determine if any differences exist in BMDMSCs acquired from the sternum and the ilium. We compared the two harvest sites in their propensity to undergo multilineage differentiation, differences in cell surface markers, or gene transduction efficiencies. BMDMSCs were isolated and culture-expanded from 5 ml aspirates of bone marrow from sternum and ilium. The cells were then plated and cultured with appropriate differentiation medium to result in multi-lineage differentiation and cell characteristics were compared between sternal and ilial samples. Cell surface antibody expression of CD11a/18, CD34, CD44, and CD90 were evaluated using flow cytometry, and gene transduction efficiencies were evaluated using GFP scAAV. There were no statistically significant differences in cell characteristics between MSCs cultured from the sternum and the ilium under any circumstances.
Publication Date: 2015-12-07 PubMed ID: 26664993PubMed Central: PMC4672231DOI: 10.3389/fvets.2015.00067Google Scholar: Lookup
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Summary
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The research examined equine mesenchymal stem cells taken from various harvesting sites, namely the sternum and the ilium, to investigate potential differences in their differentiation, surface markers, and gene transduction efficiencies. The findings revealed no significant variations in these stem cell characteristics based on their origin.
Objective of the study
- The research aimed to analyze and compare equine bone marrow-derived mesenchymal stem cells (BMDMSCs) harvested from two common locations: the sternum and the ilium. The primary focus was to ascertain any differentiations in their capability for multilineage differentiation, disparity in cell surface markers, or gene transduction efficiency.
Methods
- Cell harvest: BMDMSCs were extracted and developed from 5 ml samples of bone marrow acquired from sternum and ilium.
- Cell culture: The harvested cells were cultured in a suitable differentiation medium that encouraged multilinear differentiation.
- Comparison of cells: The characteristics of the cells cultivated from the sternum and the ilium were compared.
- Marker evaluation: The study assessed the expression of cell surface antibodies such as CD11a/18, CD34, CD44, and CD90 using flow cytometry.
- Gene transduction evalutation: The gene transduction efficiencies of the cells were measured using GFP scAAV (Green Fluorescent Protein self-complementary Adeno-Associated Virus).
Findings
- No significant variation was found in the properties of MSCs based on their harvest location (sternum or ilium). This was consistent across all tested parameters: multilineage differentiation, differences in cell surface markers, and gene transduction efficiencies.
Implications of the Study
- This research provides important insights into equine medicine, especially in the treatment of musculoskeletal system diseases. Since no significant differences were found between the stem cells derived from different harvest sites, it affirms that stem cells can be harvested from either location without affecting their therapeutic properties.
- This result encourages further exploration in the use of BMDMSCs for tissue healing and regeneration in orthopedic injuries among horses, providing potential flexibility in the choice of harvest location.
Cite This Article
APA
Lombana KG, Goodrich LR, Phillips JN, Kisiday JD, Ruple-Czerniak A, McIlwraith CW.
(2015).
An Investigation of Equine Mesenchymal Stem Cell Characteristics from Different Harvest Sites: More Similar Than Not.
Front Vet Sci, 2, 67.
https://doi.org/10.3389/fvets.2015.00067 Publication
Researcher Affiliations
- Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
- Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
- Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
- Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
- Department of Comparative Pathobiology, Purdue University , West Lafayette, IN , USA.
- Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
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