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Home / Equine Research Bank / Vet Res Commun / Apoptotic effects of tamoxifen on leukocytes from horse peri...
Veterinary research communications2013; 37(4); 333-338; doi: 10.1007/s11259-013-9571-0

Apoptotic effects of tamoxifen on leukocytes from horse peripheral blood and bronchoalveolar lavage fluid.

Abstract: A reduction in inflammatory cell apoptosis is an important concept in the maintenance of inflammation and a potential target for the resolution of inflammation in many inflammatory diseases. Dysregulation of apoptosis has been implicated in a range of diseases, including tumors, neurodegenerative disorders and autoimmunity, and may also be implicated in allergic asthma. In horses, recurrent airway obstruction (RAO) is an asthma-like condition that is characterized increased survival neutrophil bronchial. Tamoxifen is a synthetic, non-steroidal, anti-estrogen agent that is widely used for treating all stages of breast cancer and has been approved for the prevention of breast cancer in high-risk women. The observed efficacy of tamoxifen has been attributed to both growth arrest and the induction of apoptosis. Therefore, the aim of our study was to evaluate the ability of tamoxifen to induce apoptosis in vitro in granulocytic cells from peripheral blood and in mononuclear cells from bronchoalveolar lavage fluid (BALF) in horses. Flow cytometry using commercial AnnexinV-FITC and propidium iodide was used to quantify early and late apoptotic leukocytes, respectively. The results showed a significant increase in early apoptosis in peripheral blood and bronchial granulocytic cells treated with tamoxifen. The rate of early apoptosis of mononuclear cells from blood and BALF when incubated with tamoxifen was significantly lower compared with granulocytic cells. We did not observe a direct effect of tamoxifen on late apoptosis in any of the in vitro assays in the cell types used here. These results indicate that the apoptotic mechanisms under these experimental conditions would affect only blood and BALF granulocytic cells, particularly in early apoptosis. Finally, further in vitro and in vivo studies are needed to better understand apoptotic mechanisms because tamoxifen could be used to treat chronic, inflammatory pathologies associated with granulocytes and allergic diseases, such as asthma or equine RAO.
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Publication Date: 2013-07-12 PubMed ID: 23846832DOI: 10.1007/s11259-013-9571-0Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the potential of tamoxifen, a drug commonly used to treat breast cancer, to induce cell death or apoptosis in certain types of cells involved in inflammation and asthma-like conditions in horses. The findings showed that tamoxifen significantly increased early cell death in granulocytic cells (a type of white blood cell) from peripheral blood and from the lungs.

Introduction and Aim

  • The study aimed to expand our understanding of the apoptotic effects of tamoxifen on leukocytes, a type of white blood cell, sourced from horse peripheral blood and bronchoalveolar lavage fluid (BALF).
  • The researchers wanted to see if tamoxifen, known for its cancer-fighting properties, could be used to trigger apoptosis, or programmed cell death, in these inflammatory cells, potentially offering a therapeutic pathway for inflammatory and allergic diseases.

Background

  • Apoptosis, or controlled cell death, plays a crucial role in health, and a lack of apoptosis can lead to sustained inflammation and contribute to diseases like cancer, neurodegenerative disorders, and autoimmunity.
  • Interestingly, a similar consequence is suspected in allergic asthma, which in horses is known as recurrent airway obstruction (RAO) and characterized by long-lived bronchial neutrophils, a type of white blood cell.
  • Tamoxifen, an anti-estrogen drug, is popular in treating all stages of breast cancer and for preventing the same in high-risk women. The drug’s success is attributed to both growth arrest and the induction of apoptosis.

Methods and Results

  • To investigate tamoxifen’s impact on apoptosis, the researchers treated granulocytic cells from horse peripheral blood and mononuclear cells from BALF with tamoxifen, in vitro.
  • They used flow cytometry, a technique that analyzes the physical and molecular characteristics of cells, to measure early and late apoptosis in leukocytes.
  • The results revealed a significant increase in early apoptosis in blood and bronchial granulocytic cells treated with tamoxifen. However, tamoxifen did not appear to have the same impact on the mononuclear cells from blood and BALF.
  • Interestingly, the researchers did not observe a direct impact of tamoxifen on late apoptosis in any of the cell types in this in vitro experiment.

Conclusions and Future Implications

  • The results suggest that the apoptotic mechanisms influenced by tamoxifen under these experimental conditions primarily effect blood and BALF granulocytic cells, but only in early apoptosis.
  • This study is a step toward understanding how tamoxifen could potentially be used to treat chronic inflammatory pathologies connected with granulocytes and allergic diseases, such as asthma or horse RAO.
  • Additional in vitro and in vivo studies are necessary to further comprehend the apoptotic mechanisms influenced by tamoxifen and how they could be applied to treat such diseases.

Cite This Article

APA
Sarmiento J, Perez B, Morales N, Henriquez C, Vidal L, Folch H, Galecio JS, Morán G. (2013). Apoptotic effects of tamoxifen on leukocytes from horse peripheral blood and bronchoalveolar lavage fluid. Vet Res Commun, 37(4), 333-338. https://doi.org/10.1007/s11259-013-9571-0

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 37
Issue: 4
Pages: 333-338

Researcher Affiliations

Sarmiento, J
  • Department of Phisiology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile.
Perez, B
    Morales, N
      Henriquez, C
        Vidal, L
          Folch, H
            Galecio, J S
              Morán, G

                MeSH Terms

                • Animals
                • Antineoplastic Agents / pharmacology
                • Apoptosis / drug effects
                • Bronchoalveolar Lavage Fluid / cytology
                • Granulocytes / drug effects
                • Horses
                • Leukocytes / drug effects
                • Tamoxifen / pharmacology

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