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Research in veterinary science2013; 95(1); 272-277; doi: 10.1016/j.rvsc.2013.01.017

Autologous bone marrow mesenchymal stromal cells for regeneration of injured equine ligaments and tendons: a clinical report.

Abstract: The use of Mesenchymal Stromal Cells (MSCs) in orthopedic practice has recently and rapidly acquired an important role. Therapies based on the use of MSCs for the treatment of acute injuries as well as chronic inflammatory disorders are gradually becoming clinical routine. These cells have demonstrated intriguing therapeutic potentialities (i.e.: inflammation control, tissue regeneration and pathological scar prevention), that have been taken into consideration for use in both human and veterinary medicine. In particular, horses represent high performance athletes considered models for human pathologies since musculo-skeletal disorders frequently occur in this species. In the past, repair of tendon injures were performed by different methods. In particular, clinical therapy was based on ice application, bandage, box rest and controlled exercise. An alternative approach consisted on the use of corticosteroid (inflammation reduction) and other drugs (sodium hyaluronate, polysulphated glycosaminoglycans, beta aminoproprionitrile fumarate). Furthermore, surgical treatments like accessory ligament desmotomy, local irritation by line firing or pin firing were commonly used. More recently ultrasound, laser therapy, electromagnetic field therapy have been considered. Unfortunately, they did not allow complete tissue healing and quite often animals did not regain competitiveness. In order to minimize this inconvenience, the use of MSCs has been introduced as an alternative to the traditional approach since it represents a potential tool to improve tissue regeneration. Aim of this study was to evaluate the capability of MSCs to improve the functional outcome of horses affected by tendonitis and desmitis. Thirty-three breed and activity-matched horses affected by tendonitis or desmitis, were included in clinical trial scored for lesions and subdivided into two groups. Group 1 animals were treated with autologous MSCs, associated with platelet rich plasma (group 1). Bone marrow samples were collected from the sternum of the treated horses and processed in order to isolate MSCs. Following cell therapy, they were subjected to a rehabilitation period and their ability to resume training was evaluated. In this study, implanted MSCs caused no adverse reactions and thirteen out of the eighteen inoculated horses returned to race competitions. On the contrary, no improvement was seen in the twelve animals of group 2 treated with pin firing, that were not able to resume sport activity. In conclusion the clinical trial proves the safety of equine bone-marrow derived MSCs and a successful outcome of the treated animals that returned to their previous level of sport activity.
Copyright © 2013 Elsevier Ltd. All rights reserved.
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Publication Date: 2013-02-16 PubMed ID: 23419936DOI: 10.1016/j.rvsc.2013.01.017Google Scholar: Lookup
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  • Clinical Trial
  • Journal Article
  • Randomized Controlled Trial
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper is about the use of Mesenchymal Stromal Cells (MSCs), derived from horse bone marrow, in the treatment of tendonitis and desmitis in horses, showing promising results compared to traditional treatments.

Research Context and Objectives

  • The study begins by highlighting the rising importance and potential of Mesenchymal Stromal Cells (MSCs) in orthopedic practice. Their ability to control inflammation, stimulate tissue regeneration, and prevent pathological scarring has attracted researchers’ interest in the field of both human and veterinary medicine.
  • As high-performance athletes, horses are prone to musculo-skeletal disorders, making them a suitable model for such studies.
  • The researchers aimed to test the effectiveness of MSCs in improving functional outcomes for horses suffering from tendonitis and desmitis, conditions previously treated with varied methods, none of which ensured complete tissue healing or return to competitive performance.

Study Design and Methodology

  • The study involved thirty-three horses, all breed and activity-matched, suffering from tendonitis or desmitis.
  • The horses were divided into two groups. Group 1 received a treatment of autologous MSCs associated with platelet-rich plasma. The MSCs were derived from bone marrow samples collected from the horses’ sternum.
  • Group 2 horses, on the other hand, were treated with traditional methods using pin firing.
  • Post-treatment, horses underwent a rehabilitation phase and their ability to resume training was evaluated.

Results & Conclusions

  • The researchers found that the implanted MSCs caused no adverse reactions. From the eighteen horses that were treated with this method, thirteen of them were able to return to competitive racing, which is a significant success rate.
  • In stark contrast, none of the horses from Group 2, who were treated with pin firing, saw any improvement or could resume sport activity. This significant difference showed the potential benefits of using MSCs in treating these conditions over traditional methods.
  • In conclusion, the research demonstrated the safety and efficacy of using equine bone-marrow derived MSCs in treating equine musculo-skeletal disorders such as tendonitis and desmitis.

Cite This Article

APA
Renzi S, Riccò S, Dotti S, Sesso L, Grolli S, Cornali M, Carlin S, Patruno M, Cinotti S, Ferrari M. (2013). Autologous bone marrow mesenchymal stromal cells for regeneration of injured equine ligaments and tendons: a clinical report. Res Vet Sci, 95(1), 272-277. https://doi.org/10.1016/j.rvsc.2013.01.017

Publication

Research in veterinary science
ISSN: 1532-2661
NlmUniqueID: 0401300
Country: England
Language: English
Volume: 95
Issue: 1
Pages: 272-277

Researcher Affiliations

Renzi, S
  • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy. sabrina.renzi@izsler.it
Riccò, S
    Dotti, S
      Sesso, L
        Grolli, S
          Cornali, M
            Carlin, S
              Patruno, M
                Cinotti, S
                  Ferrari, M

                    MeSH Terms

                    • Animals
                    • Cell Differentiation / physiology
                    • Horse Diseases / pathology
                    • Horse Diseases / surgery
                    • Horses
                    • Male
                    • Mesenchymal Stem Cell Transplantation / methods
                    • Mesenchymal Stem Cell Transplantation / veterinary
                    • Mesenchymal Stem Cells / cytology
                    • Regenerative Medicine / methods
                    • Tendinopathy / surgery
                    • Tendinopathy / veterinary

                    Citations

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