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PloS one2019; 14(8); e0221317; doi: 10.1371/journal.pone.0221317

Comparison of efficacy and safety of single versus repeated intra-articular injection of allogeneic neonatal mesenchymal stem cells for treatment of osteoarthritis of the metacarpophalangeal/metatarsophalangeal joint in horses: A clinical pilot study.

Abstract: The purpose of this prospective study was to evaluate the effects of single and repeated intra-articular administration of allogeneic, umbilical cord-derived, neonatal mesenchymal stem cells (MSC) in horses with lameness due to osteoarthritis (OA) of a metacarpophalangeal joint (MPJ). Twenty-eight horses were included. Horses were divided into two groups. Horses in group MSC1 received an MSC injection at M0 and a placebo injection at M1 (1 month after M0). Horses in group MSC2 received MSC injections at M0 and at M1. Joint injections were performed with a blinded syringe. Clinical assessment was performed by the treating veterinarian at M1, M2 and M6 (2 and 6 months after M0), including lameness evaluation, palpation and flexion of the joint. Radiographic examination of the treated joints was performed at inclusion and repeated at M6. Radiographs were anonymized and assessed by 2 ECVDI LA associate members. Short term safety assessment was performed by owner survey. A 2-month rehabilitation program was recommended to veterinarians. There was a significant improvement of the total clinical score for horses in both groups. There was no significant difference in the total clinical score between groups MSC1 and MSC2 at any time point in the study. There was no significant difference in the total radiographic OA score, osteophyte score, joint space width score and subchondral bone score between inclusion and M6. Owner-detected adverse effects to MSC injection were recorded in 18% of the horses. Lameness caused by OA improved significantly over the 6-month duration of the study after treatment with allogeneic neonatal umbilical cord-derived MSCs combined with 8 weeks rest and rehabilitation. There is no apparent clinical benefit of repeated intra-articular administration of MSCs at a 1-month interval in horses with MPJ OA when compared to the effect of a single injection.
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Publication Date: 2019-08-29 PubMed ID: 31465445PubMed Central: PMC6715221DOI: 10.1371/journal.pone.0221317Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Multicenter Study
  • Randomized Controlled Trial
  • Veterinary
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the effects of using one-time and repeated injections of stem cells derived from the umbilical cord of newborn horses to treat osteoarthritis-induced lameness in adult horses. The findings suggest that both treatments significantly reduce lameness, with no notable difference between single and repeated injection outcomes.

Research Overview

This is a pilot clinical study conducted on 28 horses suffering from osteoarthritis (OA) in their metacarpophalangeal joint (MPJ), causing lameness. The horses were split into two groups, MSC1 and MSC2, with the MSC1 group receiving a single injection of mesenchymal stem cells (MSC) followed by a placebo injection after one month, while the MSC2 group received two MSC injections one month apart.

  • The researchers performed joint injections using a blinded syringe and did clinical evaluations at the 1-month, 2-month, and 6-month marks.
  • Radiographic examination of the affected joints was conducted at the start of the study and again six months later.
  • The study also includes data from owner surveys to evaluate any adverse effects of the treatment.

Findings

The results pointed to a significant improvement in overall clinical scores, reflecting reduced lameness, for horses in both groups. Radiographic assessment did not suggest any significant changes in the OA score, osteophyte score, joint space width score or subchondral bone score between the start of the study and the 6-month mark.

  • There was no significant difference found between the single and repeated injection groups at any point in the study.
  • 18% of the horses exhibited adverse effects to the MSC injection, as reported by the owners.
  • Despite the presence of osteoarthritis, lameness improved markedly over the 6-month period after treatment with umbilical cord-derived stem cells, coupled with 8 weeks of rest and rehabilitation.

Conclusion

Treatment with MSCs showed a significant improvement in lameness caused by osteoarthritis in horses, with no discernible difference in effectiveness between single and repeated injections. Thus, it suggests that there seems to be no apparent clinical advantage in administering repeated MSC injections at one-month intervals in horses with MPJ OA, compared to the effect of a single stem cell injection.

Cite This Article

APA
Magri C, Schramme M, Febre M, Cauvin E, Labadie F, Saulnier N, François I, Lechartier A, Aebischer D, Moncelet AS, Maddens S. (2019). Comparison of efficacy and safety of single versus repeated intra-articular injection of allogeneic neonatal mesenchymal stem cells for treatment of osteoarthritis of the metacarpophalangeal/metatarsophalangeal joint in horses: A clinical pilot study. PLoS One, 14(8), e0221317. https://doi.org/10.1371/journal.pone.0221317

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 14
Issue: 8
Pages: e0221317
PII: e0221317

Researcher Affiliations

Magri, Carmelo
  • Clinéquine, VetAgro Sup, Campus Vétérinaire de Lyon, Marcy l'Etoile, France.
Schramme, Michael
  • Clinéquine, VetAgro Sup, Campus Vétérinaire de Lyon, Marcy l'Etoile, France.
Febre, Marine
  • Vetbiobank SAS, Campus Vétérinaire de Lyon, Marcy l'Etoile, France.
Cauvin, Eddy
  • Azurvet, Cagnes-sur-Mer, France.
Labadie, Fabrice
  • Vetbiobank SAS, Campus Vétérinaire de Lyon, Marcy l'Etoile, France.
Saulnier, Nathalie
  • Vetbiobank SAS, Campus Vétérinaire de Lyon, Marcy l'Etoile, France.
François, Isé
  • Clinéquine, VetAgro Sup, Campus Vétérinaire de Lyon, Marcy l'Etoile, France.
Lechartier, Antoine
  • Clinique équine vétérinaire de Meheudin, Ecouché, France.
Aebischer, David
  • Clinique équine Areda, Bex, Switzerland.
Moncelet, Anne-Sophie
  • Clinique équine de Grosbois, Marolles-en-Brie, France.
Maddens, Stéphane
  • Vetbiobank SAS, Campus Vétérinaire de Lyon, Marcy l'Etoile, France.

MeSH Terms

  • Allografts
  • Animals
  • Female
  • Horse Diseases / pathology
  • Horse Diseases / physiopathology
  • Horse Diseases / therapy
  • Horses
  • Male
  • Mesenchymal Stem Cell Transplantation
  • Metacarpophalangeal Joint
  • Metatarsophalangeal Joint
  • Osteoarthritis / pathology
  • Osteoarthritis / physiopathology
  • Osteoarthritis / therapy

Conflict of Interest Statement

The authors have read the journal's policy and the authors of this manuscript have the following competing interests: SM is a current and principal shareholder of Vetbiobank. MF, FL, NS, are employees of Vetbiobank. There is no commercial or financial connection between Vetbiobank and the seven co-authors (CM, MS, EC, IF, AL, DA, AM). EC is shareholder of Azurvet. The product used in this study is developed for commercial use in France and Europe. There are no patents associated with this research to declare. These commercial affiliations do not alter adherence of all authors to all PLOS ONE policies on sharing data and materials. MS and CM are current employees of the National Veterinary School Of Lyon, Vetagro Sup in France.

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