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BMC musculoskeletal disorders2018; 19(1); 230; doi: 10.1186/s12891-018-2163-y

Effects of mesenchymal stromal cells versus serum on tendon healing in a controlled experimental trial in an equine model.

Abstract: Mesenchymal stromal cells (MSC) have shown promising results in the treatment of tendinopathy in equine medicine, making this therapeutic approach seem favorable for translation to human medicine. Having demonstrated that MSC engraft within the tendon lesions after local injection in an equine model, we hypothesized that they would improve tendon healing superior to serum injection alone. Methods: Quadrilateral tendon lesions were induced in six horses by mechanical tissue disruption combined with collagenase application 3 weeks before treatment. Adipose-derived MSC suspended in serum or serum alone were then injected intralesionally. Clinical examinations, ultrasound and magnetic resonance imaging were performed over 24 weeks. Tendon biopsies for histological assessment were taken from the hindlimbs 3 weeks after treatment. Horses were sacrificed after 24 weeks and forelimb tendons were subjected to macroscopic and histological examination as well as analysis of musculoskeletal marker expression. Results: Tendons injected with MSC showed a transient increase in inflammation and lesion size, as indicated by clinical and imaging parameters between week 3 and 6 (p < 0.05). Thereafter, symptoms decreased in both groups and, except that in MSC-treated tendons, mean lesion signal intensity as seen in T2w magnetic resonance imaging and cellularity as seen in the histology (p < 0.05) were lower, no major differences could be found at week 24. Conclusions: These data suggest that MSC have influenced the inflammatory reaction in a way not described in tendinopathy studies before. However, at the endpoint of the current study, 24 weeks after treatment, no distinct improvement was observed in MSC-treated tendons compared to the serum-injected controls. Future studies are necessary to elucidate whether and under which conditions MSC are beneficial for tendon healing before translation into human medicine.
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Publication Date: 2018-07-18 PubMed ID: 30021608PubMed Central: PMC6052633DOI: 10.1186/s12891-018-2163-yGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on comparing the effects of mesenchymal stromal cells (MSC) and the effects of serum alone on the healing process of tendon injuries in horses. Findings suggest that although MSC treatment initially resulted in an increased inflammation response, no significant improvement was observed in comparison to serum-only treatments after a 24 week period.

Research Methodology

  • In order to carry out the experiment, tendon lesions were induced on six horses three weeks prior to the treatment.
  • These injuries were created using a combination of mechanical disruptions and collagenase application.
  • The treatment involved injecting the affected areas with MSC suspended in serum or just serum alone.
  • Research data was continually gathered over a 24 week period through clinical examinations, ultrasound, and magnetic resonance imaging.
  • To gather more detailed data on the effects of the treatments, tendon biopsies were collected three weeks post-treatment from the hind limbs of the horses.

Findings and Analysis

  • Interestingly, tendon lesions that were treated with MSC showed an initial increase in inflammation and lesion size between week 3 and 6 after the treatment.
  • This was determined through assessing multiple parameters in clinical and imaging data.
  • Subsequently, symptoms began to decrease in both the group of tendons treated with MSC and in the control group (treated with serum alone).
  • Although the inflammation and cellularity levels in the tendons treated with MSC were found to be lower at the end of the 24th week, no significant differences were observed in the overall health of the tendons between the two groups.

Conclusions and Implications

  • The research concluded that while MSC did influence the inflammation process in tendon healing, there was no significant improvement in the tendon healing process when compared to the control group after a period of 24 weeks.
  • Therefore, while MSC have previously shown promising results in equine tendon healing, this study highlights the need for further research to confirm their complete effectiveness and applicability in human medicine.
  • Additional studies are necessary to determine the exact conditions under which MSC can be beneficial for tendon healing in both equine and human subjects.

Cite This Article

APA
Ahrberg AB, Horstmeier C, Berner D, Brehm W, Gittel C, Hillmann A, Josten C, Rossi G, Schubert S, Winter K, Burk J. (2018). Effects of mesenchymal stromal cells versus serum on tendon healing in a controlled experimental trial in an equine model. BMC Musculoskelet Disord, 19(1), 230. https://doi.org/10.1186/s12891-018-2163-y

Publication

BMC musculoskeletal disorders
ISSN: 1471-2474
NlmUniqueID: 100968565
Country: England
Language: English
Volume: 19
Issue: 1
Pages: 230
PII: 230

Researcher Affiliations

Ahrberg, A B
  • Department of Orthopedics, Traumatology and Plastic Surgery, University of Leipzig, Liebigstr. 20, 04103, Leipzig, Germany. annette.ahrberg@medizin.uni-leipzig.de.
  • Translational Center for Regenerative Medicine (TRM), University of Leipzig, Leipzig, Germany. annette.ahrberg@medizin.uni-leipzig.de.
Horstmeier, C
  • Translational Center for Regenerative Medicine (TRM), University of Leipzig, Leipzig, Germany.
  • Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Leipzig, Germany.
  • University Equine Hospital, University of Leipzig, Leipzig, Germany.
Berner, D
  • Department of Clinical Science and Services, The Royal Veterinary College, University of London, London, UK.
Brehm, W
  • Translational Center for Regenerative Medicine (TRM), University of Leipzig, Leipzig, Germany.
  • Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Leipzig, Germany.
  • University Equine Hospital, University of Leipzig, Leipzig, Germany.
Gittel, C
  • University Equine Hospital, University of Leipzig, Leipzig, Germany.
Hillmann, A
  • Translational Center for Regenerative Medicine (TRM), University of Leipzig, Leipzig, Germany.
  • Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Leipzig, Germany.
Josten, C
  • Department of Orthopedics, Traumatology and Plastic Surgery, University of Leipzig, Liebigstr. 20, 04103, Leipzig, Germany.
Rossi, G
  • School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Schubert, S
  • Translational Center for Regenerative Medicine (TRM), University of Leipzig, Leipzig, Germany.
  • Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Leipzig, Germany.
  • Institute of Veterinary Physiology, University of Leipzig, Leipzig, Germany.
Winter, K
  • University Equine Hospital, University of Leipzig, Leipzig, Germany.
  • Institute of Anatomy, Medical Faculty, University of Leipzig, Leipzig, Germany.
Burk, J
  • Translational Center for Regenerative Medicine (TRM), University of Leipzig, Leipzig, Germany.
  • Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Leipzig, Germany.
  • Institute of Veterinary Physiology, University of Leipzig, Leipzig, Germany.
  • Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.

MeSH Terms

  • Animals
  • Cells, Cultured
  • Disease Models, Animal
  • Female
  • Follow-Up Studies
  • Horses
  • Male
  • Mesenchymal Stem Cell Transplantation / methods
  • Mesenchymal Stem Cell Transplantation / trends
  • Serum
  • Tendinopathy / diagnostic imaging
  • Tendinopathy / therapy

Grant Funding

  • BMBF 1315883 / German Federal Ministry of Education and Research
  • not applicable / Saxon Ministry of Science and the Fine Arts

Conflict of Interest Statement

ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Approval of the local ethics committee (Landesdirektion Leipzig, TVV 34/13) had been given beforehand. No human participants have been involved. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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