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Home / Equine Research Bank / J Orthop Res / Enhanced early chondrogenesis in articular defects following...
Journal of orthopaedic research : official publication of the Orthopaedic Research Society2007; 25(7); 913-925; doi: 10.1002/jor.20382

Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model.

Abstract: Mesenchymal stem cells (MSCs) provide an important source of pluripotent cells for musculoskeletal tissue repair. This study examined the impact of MSC implantation on cartilage healing characteristics in a large animal model. Twelve full-thickness 15-mm cartilage lesions in the femoropatellar articulations of six young mature horses were repaired by injection of a self-polymerizing autogenous fibrin vehicle containing mesenchymal stem cells, or autogenous fibrin alone in control joints. Arthroscopic second look and defect biopsy was obtained at 30 days, and all animals were euthanized 8 months after repair. Cartilage repair tissue and surrounding cartilage were assessed by histology, histochemistry, collagen type I and type II immunohistochemistry, collagen type II in situ hybridization, and matrix biochemical assays. Arthroscopic scores for MSC-implanted defects were significantly improved at the 30-day arthroscopic assessment. Biopsy showed MSC-implanted defects contained increased fibrous tissue with several defects containing predominantly type II collagen. Long-term assessment revealed repair tissue filled grafted and control lesions at 8 months, with no significant difference between stem cell-treated and control defects. Collagen type II and proteoglycan content in MSC-implanted and control defects were similar. Mesenchymal stem cell grafts improved the early healing response, but did not significantly enhance the long-term histologic appearance or biochemical composition of full-thickness cartilage lesions.
Copyright (c) 2007 Orthopaedic Research Society.
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Publication Date: 2007-04-04 PubMed ID: 17405160DOI: 10.1002/jor.20382Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the effect of mesenchymal stem cell implantation to improve the healing of cartilage tissue in horses. Although initial results showed improvement, there were no significant long-term benefits compared to a control group.

Objectives and Methods

  • The study’s primary aim was to evaluate the potential benefits of using mesenchymal stem cells (MSCs) for repairing musculoskeletal tissue injuries, specifically cartilage lesions in horses.
  • A sample of twelve full-thickness cartilage lesions from six young adult horses was selected for this. The lesions were treated with either MSCs combined with a self-polymerizing autogenous fibrin vehicle or only with autogenous fibrin, which served as the control treatment.
  • The MSC-treated and control lesions were assessed and compared at two separate stages: firstly at the 30-day mark, and secondly eight months after the lesions were treated.

Measurements and Results

  • Several methods were used to evaluate and compare the lesions, including histology, histochemistry, immunohistochemistry for detecting collagen types I and II, in situ hybridization for collagen type II, and matrix biochemical assays.
  • Arthroscopic scores for MSC-implanted defects showed significant improvement 30 days post-treatment. Biopsies revealed that MSC-treated defects had increased fibrous tissue and, in several cases, were filled predominantly with type II collagen.
  • However, this early beneficial effect didn’t persist. At the eight-month mark, it was found that both the MSC-treated and control lesions were filled with repair tissue but there was no significant difference between the two groups in their histological appearance or biochemical composition.
  • Collagen type II and proteoglycan content, significant markers of cartilage repair, were found to be similar in both the MSC-implanted and control defects.

Conclusion

  • While MSCs seemed to facilitate the early healing response of cartilage defects, they did not significantly enhance the long-term healing attributes. Consequently, the research concluded that MSC grafts, despite their early promise, did not deliver significantly better long-term results in terms of histological appearance or biochemical composition of full-thickness cartilage lesions compared to the control group.

Cite This Article

APA
Wilke MM, Nydam DV, Nixon AJ. (2007). Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model. J Orthop Res, 25(7), 913-925. https://doi.org/10.1002/jor.20382

Publication

Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 0736-0266
NlmUniqueID: 8404726
Country: United States
Language: English
Volume: 25
Issue: 7
Pages: 913-925

Researcher Affiliations

Wilke, Markus M
  • Comparative Orthopaedics Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, C3-187, Ithaca, New York 14853, USA.
Nydam, Daryl V
    Nixon, Alan J

      MeSH Terms

      • Animals
      • Arthroscopy
      • Biomarkers / metabolism
      • Cartilage, Articular / injuries
      • Cartilage, Articular / pathology
      • Chondrogenesis / physiology
      • Collagen Type I / metabolism
      • Collagen Type II / genetics
      • Collagen Type II / metabolism
      • Disease Models, Animal
      • Extracellular Matrix / chemistry
      • Female
      • Horses
      • In Situ Hybridization
      • Male
      • Mesenchymal Stem Cell Transplantation
      • Proteoglycans / metabolism
      • RNA, Messenger / metabolism
      • Stifle
      • Wound Healing / physiology

      Citations

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