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Journal of cellular and molecular medicine2016; 20(12); 2384-2404; doi: 10.1111/jcmm.12932

Equine metabolic syndrome impairs adipose stem cells osteogenic differentiation by predominance of autophagy over selective mitophagy.

Abstract: Adipose-derived mesenchymal stem cells (ASC) hold great promise in the treatment of many disorders including musculoskeletal system, cardiovascular and/or endocrine diseases. However, the cytophysiological condition of cells, used for engraftment seems to be fundamental factor that might determine the effectiveness of clinical therapy. In this study we investigated growth kinetics, senescence, accumulation of oxidative stress factors, mitochondrial biogenesis, autophagy and osteogenic differentiation potential of ASC isolated from horses suffered from equine metabolic syndrome (EMS). We demonstrated that EMS condition impairs multipotency/pluripotency in ASCs causes accumulation of reactive oxygen species and mitochondria deterioration. We found that, cytochrome c is released from mitochondria to the cytoplasm suggesting activation of intrinsic apoptotic pathway in those cells. Moreover, we observed up-regulation of p21 and decreased ratio of Bcl-2/BAX. Deteriorations in mitochondria structure caused alternations in osteogenic differentiation of ASC resulting in their decreased proliferation rate and reduced expression of osteogenic markers BMP-2 and collagen type I. During osteogenic differentiation of ASC , we observed autophagic turnover as probably, an alternative way to generate adenosine triphosphate and amino acids required to increased protein synthesis during differentiation. Downregulation of PGC1α, PARKIN and PDK4 in differentiated ASC confirmed impairments in mitochondrial biogenesis and function. Hence, application of ASC into endocrinological or ortophedical practice requires further investigation and analysis in the context of safeness of their application.
© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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Publication Date: 2016-09-14 PubMed ID: 27629697PubMed Central: PMC5134411DOI: 10.1111/jcmm.12932Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research evaluates the impact of Equine Metabolic Syndrome (EMS) on adipose-derived mesenchymal stem cells (ASC) in horses. The study found that EMS impairs the pluripotency of ASCs leading to the deterioration of mitochondria, reactive oxygen species accumulation, and disruption of the osteogenic differentiation process.

Stem Cell Potential and Equine Metabolic Syndrome

  • The research focused on adipose-derived mesenchymal stem cells (ASCs), which show potential in treating a range of disorders, including those related to musculoskeletal, cardiovascular, and endocrine systems. The state of the cells utilized for engraftment could determine the effectiveness of clinical treatments.
  • Researchers examined the growth kinetics, senescence, oxidative stress factors accumulation, mitochondrial biogenesis, osteogenic differentiation potential, and autophagy of ASCs from horses with Equine Metabolic Syndrome (EMS), a metabolic disorder in horses that is akin to metabolic syndrome in humans.
  • The findings showed that EMS hampers multipotency/pluripotency in ASCs, leading to reactive oxygen species accumulation and mitochondrial deterioration. There was also evidence of the intrinsic apoptotic pathway activation in the cells.

Implications on Osteogenic Differentiation

  • The study observed that ASCs from horses with EMS exhibited realized disruptions in osteogenic differentiation that resulted in a decreased proliferation rate and reduced the expression of osteogenic markers such as BMP-2 and collagen type I.
  • During osteogenic differentiation of ASCs, autophagic turnover was observed. This process, possibly an alternate way to generate adenosine triphosphate and amino acids needed for increased protein synthesis during differentiation, is negatively affected in cells suffering from EMS.
  • Persistent downregulation of PGC1α, PARKIN, and PDK4 in differentiated ASCs confirmed impairments in mitochondrial biogenesis and function.

Applications and Further Research

  • The results of the study suggest that the application of ASCs into endocrinological or orthopedical practice requires further research. The pathology associated with EMS can negatively influence the pluripotency of ASCs and affect their therapeutic potential.
  • The exploration of ways to counteract the negative impacts of diseases like EMS on ASCs could lead to more effective clinical therapies using stem cells.

Cite This Article

APA
Marycz K, Kornicka K, Marędziak M, Golonka P, Nicpoń J. (2016). Equine metabolic syndrome impairs adipose stem cells osteogenic differentiation by predominance of autophagy over selective mitophagy. J Cell Mol Med, 20(12), 2384-2404. https://doi.org/10.1111/jcmm.12932

Publication

Journal of cellular and molecular medicine
ISSN: 1582-4934
NlmUniqueID: 101083777
Country: England
Language: English
Volume: 20
Issue: 12
Pages: 2384-2404

Researcher Affiliations

Marycz, Krzysztof
  • Electron Microscopy Laboratory, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences Wroclaw, Wroclaw, Poland.
  • Wroclaw Research Centre EIT+, Wrocław, Poland.
Kornicka, Katarzyna
  • Electron Microscopy Laboratory, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences Wroclaw, Wroclaw, Poland.
  • Wroclaw Research Centre EIT+, Wrocław, Poland.
Marędziak, Monika
  • Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, University of Environmental and Life Sciences Wroclaw, Wroclaw, Poland.
Golonka, Paweł
  • Equine Clinic Equivet, Gliwice, Poland.
Nicpoń, Jakub
  • Department of Surgery, Faculty of Veterinary Medicine, University of Environmental and Life Sciences Wroclaw, Wroclaw, Poland.

MeSH Terms

  • Adipose Tissue / pathology
  • Animals
  • Autophagy
  • Cell Differentiation
  • Cell Proliferation
  • Cell Shape
  • Cells, Cultured
  • Female
  • Flow Cytometry
  • Horses
  • Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
  • Immunophenotyping
  • Kinetics
  • Male
  • Metabolic Syndrome / metabolism
  • Methylation
  • Mitochondria / metabolism
  • Mitochondrial Dynamics
  • Mitophagy
  • Multipotent Stem Cells / cytology
  • Osteoblasts / metabolism
  • Osteoblasts / pathology
  • Osteogenesis
  • Oxidative Stress
  • Reactive Oxygen Species / metabolism
  • Stem Cells / metabolism
  • Stem Cells / pathology
  • Stem Cells / ultrastructure
  • Transcription Factors / metabolism

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