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Home / Equine Research Bank / Animals (Basel) / Equine Stomach Development in the Foetal Period of Prenatal ...
Animals : an open access journal from MDPI2022; 13(1); 161; doi: 10.3390/ani13010161

Equine Stomach Development in the Foetal Period of Prenatal Life-An Immunohistochemical Study.

Abstract: The study consisted of the immunohistochemical analysis of fundic and pyloric mucosa in the equine stomach between the 4th and 11th month of gestation. The accessible material was classified into three age groups using the CRL method. The adult reference group was used to define potential differences between foetal and adult populations of gastric APUD cells. The samples were preserved, prepared, and stained according to the standard protocols. The immunohistochemical reaction was assessed using the semi-quantitative IRS method. The results were documented and statistically analysed. The most significant increase was seen in gastrin (G) cell activity. The activity of other endocrine cells (cholecystokinin (I) cells, somatostatin (D) cells, and somatotropin receptor (SR) cells) was less dynamic. This study proved that the development of APUD cells within the stomach mucosa undergoes quantitative and qualitative changes during stomach development. Our results correspond with the findings described in the accessible literature and prove a strong correlation between morphological changes in the stomach wall and the organ development, growth, and maturation.
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Publication Date: 2022-12-31 PubMed ID: 36611768PubMed Central: PMC9817933DOI: 10.3390/ani13010161Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines how the stomach lining in horse fetuses develops from the fourth to eleventh month of gestation. Making use of immunohistochemical analysis, it finds that various gastric APUD cells undergo significant changes in both quantity and quality during stomach development.

Methodology

  • The researchers used immunohistochemical analysis to scrutinize the fundic and pyloric mucosa of the equine stomach from the fourth to the eleventh month of gestation.
  • The samples utilized in the study were divided into three age groups, as based on the CRL (Crown Rump Length) method.
  • A comparison was made between the fetal samples and a reference group consisting of adult samples, with the intent to identify possible differences between the two groups particularly in the population of gastric APUD (Amine Precursor Uptake and Decarboxylation) cells.
  • The samples taken from the stomachs were preserved, prepared for analysis, and stained following standard protocols.
  • The researchers used the IRS (Immune-Reactive Score) method to evaluate the immunohistochemical reactions.
  • The study results were then documented and subjected to statistical analysis.

Results

  • The most significant changes were noted in the activity of gastrin (G) cells.
  • While there was noted activity in other endocrine cells – such as cholecystokinin (I) cells, somatostatin (D) cells, and somatotropin receptor (SR) cells – it was less dynamic when compared to the G cells.
  • The findings confirm that the development of APUD cells in the stomach’s mucosa is marked by quantitative and qualitative changes.

Conclusion

  • The research’s conclusions are consistent with prior findings detailed in existing literature.
  • It successfully demonstrates a strong correlation between the morphological changes observed in the stomach wall and the stomach’s development, growth, and maturity.

Cite This Article

APA
Poradowski D, Chrószcz A. (2022). Equine Stomach Development in the Foetal Period of Prenatal Life-An Immunohistochemical Study. Animals (Basel), 13(1), 161. https://doi.org/10.3390/ani13010161

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 1
PII: 161

Researcher Affiliations

Poradowski, Dominik
  • Department of Animal Physiology and Biostructure, Division of Animal Anatomy, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Kożuchowska 1, 51-631 Wrocław, Poland.
Chrószcz, Aleksander
  • Department of Animal Physiology and Biostructure, Division of Animal Anatomy, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Kożuchowska 1, 51-631 Wrocław, Poland.

Conflict of Interest Statement

The authors declare no conflict of interest.

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