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Evaluating plasma lactate running speed derived parameters for predicting maximal lactate steady state in teaching horses.
Abstract: In humans, there is an ongoing academic discussion about measuring plasma lactate concentration ([La]) during an incremental exercise test (IET) to establish thresholds that can predict the maximal lactate steady state (MLSS). Training studies on horses have utilized the onset of [La] (OBLA), also known as V, the velocity at which the [La] of 4 mM is reached. This study assesses the effectiveness of four methods for estimating the MLSS in teaching horses. Ten teaching horses underwent a single IET to determine four exercise intensity thresholds for predicting MLSS and performed several constant intensity running bouts to obtain the MLSS. The velocity corresponding to the visual lactate threshold (V) reached by the horses during IET was the intensity in the first bout. A randomized and blind trial used a visual analysis (LT) and a bi-segmented linear regression model (LT). The agreement among the velocities corresponding to the V, V, V, and V and the MLSS (V) was established using mean difference (MD), ordinary least products (OLP), and correlation coefficient (r). The average plasma [La] at MLSS was 1.50 ± 0.37 mM, and the V was higher than V with an MD of 2.12 ± 0.59 m/s between them. V and V showed the lowest mean bias when compared to the V. The V threshold, as determined with the IET protocol used, is unsuitable for estimating MLSS. In the exercise test protocol used in this study, predicting the V based on V, V, or V may be more accurate.
Copyright © 2025. Published by Elsevier Inc.
Publication Date: 2025-02-14 PubMed ID: 39956344DOI: 10.1016/j.jevs.2025.105385Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research is about evaluating different methods of estimating the Maximal Lactate Steady State (MLSS) in teaching horses, which is important in assessing their physical performance. It critically examines four specific exercise intensity thresholds derived from speed and plasma lactate concentration at which MLSS is obtained.
Overview of the Study
- The study involved ten teaching horses which performed an Incremental Exercise Test (IET) to observe their physical responses, specifically plasma lactate concentration.
- The IET was designed to establish four different exercise intensity thresholds that could potentially predict the MLSS. The intensity for the first round of running bouts was determined by the velocity at which the horses reached the visually observed lactate threshold (V) during the IET.
- The experiment was conducted in a randomized and blind set-up to ensure unbiased results.
Applied Methodology
- The researchers made use of both visual analysis (LT), and a technique called bi-segmented linear regression model (LT), to analyze the lactate threshold data obtained.
- The consistency among the velocities corresponding to the four thresholds and the MLSS (V) was verified using ordinary least products (OLP), mean difference (MD), and correlation coefficient (r).
Findings and Conclusion
- The research found that the average plasma lactate concentration at MLSS was 1.50 ± 0.37 mM.
- The velocity (V) was higher than the velocity at the lactate threshold with a mean difference of 2.12 ± 0.59 m/s.
- The lowest mean bias in comparison to the lactate threshold velocity (V) was displayed by two different velocities, V and V.
- The study concluded that the lactate threshold velocity threshold determined via IET could be inappropriate for accurately predicting MLSS.
- Instead, based on the specific exercise test protocol applied in the study, estimating the lactate threshold velocity based on other velocity markers may provide better accuracy.
Cite This Article
APA
Littiere TO, Costa GB, Sales NAA, Carvalho JRG, Rodrigues IDM, Ramos GV, Ferraz GC.
(2025).
Evaluating plasma lactate running speed derived parameters for predicting maximal lactate steady state in teaching horses.
J Equine Vet Sci, 105385.
https://doi.org/10.1016/j.jevs.2025.105385 Publication
Researcher Affiliations
- Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Animal Morphology and Physiology, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Jaboticabal 14884-900, São Paulo, Brazil; Federal University of Piauí (UFPI), Campus Professora Cinobelina Elvas (CPCE), Bom Jesus, Piauí, Brazil.
- Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Animal Morphology and Physiology, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Jaboticabal 14884-900, São Paulo, Brazil.
- Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Animal Morphology and Physiology, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Jaboticabal 14884-900, São Paulo, Brazil.
- Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Animal Morphology and Physiology, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Jaboticabal 14884-900, São Paulo, Brazil.
- Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Animal Morphology and Physiology, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Jaboticabal 14884-900, São Paulo, Brazil.
- Laboratory of Equine Sports Medicine (LMEE), Department of Clinic and Veterinary Surgery, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Jaboticabal 14884-900, São Paulo, Brazil.
- Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Animal Morphology and Physiology, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Jaboticabal 14884-900, São Paulo, Brazil. Electronic address: guilherme.c.ferraz@unesp.br.
Conflict of Interest Statement
Declaration of competing interest None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper.
Citations
This article has been cited 1 times.- Zuluaga-Cabrera AM, Barbosa da Costa G, Martinez ID, Arias MP. Defining the Heart Rate Zone Corresponding to the Lactate Threshold in Colombian Paso Horses. Animals (Basel) 2025 Nov 17;15(22).
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