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Animals : an open access journal from MDPI2021; 11(1); 155; doi: 10.3390/ani11010155

Gastrointestinal Parasitism in Miranda Donkeys: Epidemiology and Selective Control of Strongyles Infection in the Northeast of Portugal.

Abstract: In Portugal, equine parasitism in pasture animals is characterized by high parasitic burden and high helminthic biodiversity; both factors are potentially pathogenic for their hosts. The decrease in the number of donkeys over the last years in Portugal, their importance in rural lowland and mountain ecosystems and pastures and the scarce information regarding their parasitism led to this research, which aimed to evaluate the parasitological status of a Miranda donkey breed population, a native breed mainly located in the northeast of Portugal. This study provides better knowledge of their gastrointestinal parasitism, particularly strongyles, and the assessment of a targeted selective treatment (TST) as an alternative control approach of their parasitism. A longitudinal observational study was developed during a period of five years in a population of 62 Miranda donkeys. At first, strategic deworming of these animals was performed every semester, but this was progressively replaced by a TST approach according to the levels of Eggs per Gram (EPG). This new deworming strategy was conducted in association with a regular parasitological monitoring of the animals every three months, being dewormed with ivermectin when egg shedding was higher than 500 EPG. Over the study period, a decrease of the annual prevalence rate of infection by gastrointestinal strongyles was observed, from 35.5% to 19.4%, as well as a negative binomial distribution of parasitic strongyles in donkeys submitted to selective anthelminthic control. The prevalence rate of infection was higher in females (39.5%), in individuals under four years (46.7%) and in those presenting a lower body condition (40.8%). The egg output was higher in animals younger than four years (589.3 EPG) than in those older than 10 years (533.6 EPG) ( < 0.05). However, no differences were observed according to sex during the study period. Results from this study allowed to note the influence of swampy pastures and of the weather changes in the epidemiology of strongylosis in Miranda donkeys. Moreover, it was possible to establish the annual epidemiological curve of strongyle egg shedding, with June being the month with the highest EPG, December having the lowest EPG and March and September showing intermediate numbers. Overall, a lower biodiversity of gastrointestinal parasites was observed. sensu lato was the most prevalent genus and was the most observed large strongyle of the Strongylidae family. and sp. were other nematodes with a minor frequency. The higher prevalence of strongyles at the beginning of the study showed a progressive decrease throughout the research period, and also for parasite biodiversity. Therefore, a targeted selective treatment seems to be a rational anthelminthic control approach in Miranda donkey strongyle infection and in other gastrointestinal parasites, since it reduces the antiparasitic treatments, the parasite' prevalence and the EPG level. However, a loss of parasite biodiversity was noted at the end of the study period, as Cyathostominae were the only isolated strongyles. This can be a challenging situation in the long run, taking the ability of these nematodes to adapt easily to any deworming program, meaning that fecal EPG monitoring should be kept as a rule to a rational parasite control program.
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Publication Date: 2021-01-11 PubMed ID: 33440886PubMed Central: PMC7827216DOI: 10.3390/ani11010155Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research scrutinises the parasitic loads in the Miranda donkey breed in Portugal, particularly concerning the presence of strongyles worms, and finds that a targeted selective treatment approach can effectively manage parasitic burdens.

Research Overview

  • The study was conducted on the Miranda donkey breed, native to the northeastern region of Portugal. The initiating factor for the research was the considerable decrease in donkey numbers in the country, and the integral role they play in rural lowland and mountain ecosystems – while contending with noteworthy parasitic burdens.
  • The work was designed to investigate the breed’s status with regards to parasitic loads, specifically the prevalence of gastrointestinal parasites, or strongyles, which can negatively impact the health of these equines.
  • The research was conducted over a five-year period, encompassing a population of 62 Miranda donkeys.

Data Collection and Treatment Method

  • Initially, the entire group of donkeys was given prophylactic deworming treatment on a six-monthly (semester) basis, but researchers eventually transitioned this to a targeted selective treatment (TST) approach instead.
  • The TST methodology was tied to the observation of “Eggs per Gram” (EPG) counts in the animals’ feces. This acted as an indicator of the level of parasitic infestation.
  • When egg shedding was found to surpass 500 EPG in an individual, it was treated with ivermectin, an anti-parasitic medication.
  • Regular monitoring of the donkeys’ parasitic load was carried out every three months across the span of the study.

Key Findings

  • Results demonstrated a reduction in the annual prevalence of gastrointestinal strongyles infection from 35.5% to 19.4%.
  • Other findings showed that female donkeys, those under four years old, and those with a lower body condition had higher infection rates.
  • The egg output was proportionally higher in animals under the age of four, while older donkeys exhibited a lower EPG count.
  • June was found to be the month with the highest EPG count, followed by intermediate numbers in March and September, and the lowest EPG count was documented in December.
  • Some degree of parasite biodiversity was observed at the initiation of the study but saw a reduction over the study term. Strongyles were particularly prevalent but saw a noticeable decrease over the assessment period as per researchers’ records.

Conclusion and Implications

  • The study suggests that a targeted selective treatment approach can be effective to manage and control equine strongyle infection, along with other gastrointestinal parasites.
  • This approach also minimises the use of anti-parasitic treatments and contributes to a reduction in both parasite prevalence and EPG levels.
  • However, the loss of parasite biodiversity observed through this approach was flagged as a potential long-term concern, given the adaptability of certain nematodes to deworming programs. Ongoing monitoring is therefore proposed for effective management.

Cite This Article

APA
Ramalho Sousa S, Anastácio S, Nóvoa M, Paz-Silva A, Madeira de Carvalho LM. (2021). Gastrointestinal Parasitism in Miranda Donkeys: Epidemiology and Selective Control of Strongyles Infection in the Northeast of Portugal. Animals (Basel), 11(1), 155. https://doi.org/10.3390/ani11010155

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 1
PII: 155

Researcher Affiliations

Ramalho Sousa, Sérgio
  • Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal.
  • CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.
Anastácio, Sofia
  • Vasco da Gama Research Centre (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, Avenida José R. Sousa Fernandes 197 Lordemão, 3020-210 Coimbra, Portugal.
  • Centre of Neuroscience and Cell Biology (CNC), University of Coimbra, 3004-531 Coimbra, Portugal.
Nóvoa, Miguel
  • Association for the Study and Protection of Donkeys (AEPGA), Largo da Igreja n.º 48, 5225-011 Miranda do Douro, Portugal.
Paz-Silva, Adolfo
  • Control of Parasites Group (COPAR, GI-2120). Department of Animal Pathology, Veterinary Faculty, University of Santiago de Compostela, 27002 Lugo, Spain.
Madeira de Carvalho, Luís Manuel
  • CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.

Grant Funding

  • SFRH/BD/65407/2009 / Fundau00e7u00e3o para a Ciu00eancia e a Tecnologia
  • UIDP/00276/2020 / Fundau00e7u00e3o para a Ciu00eancia e Tecnologia

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 107 references
  1. FAOSTAT—Food and Agriculture Organization Corporate Statistical Database (2015)—Food and Agriculture Organization of the United Nations Statistics Division. [(accessed on 7 November 2015)]; Available online: faostat3.fao.org/browse/Q/QA/E.
  2. Arsenos G, Gelasakis A.I., Papadopoulos E. The status of donkeys (Equus asinus) in Greece. J. Hell. Vet. Med. Soc. 2010;61:212–219.
    doi: 10.12681/jhvms.14888google scholar: lookup
  3. Kugler W, Grunenfelder H, Broxham E. Donkey Breeds in Europe Inventory, Description, Need for Action, Conservation. Werner Stamm Foundation for the Support of Rare Equines; Oberwil, Switzerland: Margarethe & Rudolf Gsell Foundation; Basel, Switzerland: Bristol Foundation; Zurich, Switzerland: 2007. p. 62. Report 2007/2008.
  4. Blakeway S. The multi-dimensional donkey in landscapes of donkey-human interaction. Relat. Beyond Anthr. 2014;2:59–77.
    doi: 10.7358/rela-2014-001-blakgoogle scholar: lookup
  5. FAO—Food and Agriculture Organization of the United Nations—Draught animal power... An Overview. Agricultural Engineering Branch. Agricultural Support Systems Division. [(accessed on 7 December 2015)]; Available online: www.fao.org/ag/ags/agse/chapterps1/chapterps1-e.htm.
  6. Barbosa J.C. O gado asinino em Trás-os-Montes. Contribuição para o conhecimento da sua importância socio-económica. Proceedings of the V Colóquio Hispano-Português de Estudos Rurais; Bragança, Portugal. 23–24 October 2003; p. 12.
  7. Bacelar A.C. A utilização do Burro (Equus asinus) como recurso ecoturístico. Faculdade de Ciências, Universidade do Porto; Porto, Portugal: 2006.
  8. AEPGA—Associação para o Estudo e Protecção do Gado Asinino Raça Asinina de Miranda. [(accessed on 25 June 2015)]; Available online: www.aepga.pt. (In Portuguese)
  9. Hosseini S.H., Meshgi B., Eslami A., Bokai S., Sobhani M., Ebrahimi R.A. Prevalence and biodiversity of helminth parasites in donkeys (Equus asinus) in Iran. Int. J. Vet. Res. 2009;3:95–99.
  10. Chitra R, Rajedran S, Prasanna D, Kirubakaran A. Influences of age on the prevalence of parasitic infections among donkeys in Erode district, Tamilnandu, India. Vet. World. 2011;4:258–259.
    doi: 10.5455/vetworld.4.258google scholar: lookup
  11. Swann W.J. Improving the welfare of working equine animals in developing countries. Appl. Anim. Behav. Sci. 2006;100:148–151.
    doi: 10.1016/j.applanim.2006.04.001google scholar: lookup
  12. Waqas M, Nawaz M.S., Sajid M, Ahmad Z, Mushtaq A, Jabbar A, Zubair M. Strongylosis (red worms infestation); a potential threat to donkey’s health and performance. Glob. Vet. 2015;14:345–350.
  13. Yilma J.M., Feseha G., Svendsen E.D., Mohammed A. Health problems of working donkeys in Debre Zeit and Menagesha Regions of Ethiopia. In: Fielding D., Pearson R.A., editors. Donkeys, Mules and Horses in Tropical Agricultural Development. University of Edinburgh; Edinburgh, UK: 1991. p. 336.
  14. Bucknell D, Hoste H, Gasser RB, Beveridge I. The structure of the community of strongyloid nematodes of domestic equids.. J Helminthol 1996 Sep;70(3):185-92.
    doi: 10.1017/S0022149X0001539Xpubmed: 8960216google scholar: lookup
  15. Lyons ET, Drudge JH, Tolliver SC. Larval cyathostomiasis.. Vet Clin North Am Equine Pract 2000 Dec;16(3):501-13.
    doi: 10.1016/S0749-0739(17)30092-5pubmed: 11219346google scholar: lookup
  16. Ayele G, Feseha G, Bojia E, Joe A. Prevalence of gastro-intestinal parasites of donkeys in Dugda Bora District, Ethiopia. Livest. Res. Rural Dev. 2006;18:136.
  17. Waqas M, Khan M.S., Durrani A.Z., Khan M.A., Avais M., Khan S.A., Rehman S.U., Hussain A., Nasir A., Hussain A. Prevalence of gastrointestinal parasites, chemotherapy and haematology of strongylosis in donkeys of district Lahore, Pakistan. Int. J. Curr. Microbiol. Appl. Sci. 2014;3:198–207.
  18. Medica DL, Sukhdeo MV. Estimating transmission potential in gastrointestinal nematodes (order: Strongylida).. J Parasitol 2001 Apr;87(2):442-5.
    doi: 10.1645/0022-3395(2001)087[0442:ETPIGN]2.0.CO;2pubmed: 11318583google scholar: lookup
  19. Soulsby L, Trawford AF, Matthews JB, de Aluja AS, Dorchies P, Gebreab F, Pangui LJ, Krecek RC. Donkey: hero or villain of the parasite world? Past, present and future.. Vet Parasitol 2004 Oct 28;125(1-2):43-58.
    pubmed: 15536695doi: 10.1016/j.vetpar.2004.05.004google scholar: lookup
  20. Javed K, Ijaz M, Ali M.M., Khan I, Mehmood K, Ali S. Prevalence and hematology of tick borne hemoparasitic diseases in equines in and around Lahore. Pak. J. Zool. 2014;46:401–408.
  21. Martin Curran M, Feseha G, Smith DG. The impact of access to animal health services on donkey health and livelihoods in Ethiopia.. Trop Anim Health Prod 2005 Nov;37 Suppl 1:47-65.
    doi: 10.1007/s11250-005-9008-zpubmed: 16335071google scholar: lookup
  22. Krecek RC, Waller PJ. Towards the implementation of the "basket of options" approach to helminth parasite control of livestock: emphasis on the tropics/subtropics.. Vet Parasitol 2006 Jul 31;139(4):270-82.
    doi: 10.1016/j.vetpar.2006.04.018pubmed: 16764993google scholar: lookup
  23. Krecek R.C. Donkey parasites: Detect, diagnose, delete. Proceedings of the Second Donkey Welfare Symposium. UC Davis; Davis, CA, USA: 2014. Lecture Series 2.
  24. Asefa Z, Kumsa B, Endebu B, Gizachew A, Merga T, Debrla E. Endoparasites of donkeys in Sululta and Gefersa Districts of Central Oromia, Ethiopia. J. Anim. Vet. Adv. 2011;10:1850–1854.
    doi: 10.3923/javaa.2011.1850.1854google scholar: lookup
  25. Nielsen MK. Sustainable equine parasite control: perspectives and research needs.. Vet Parasitol 2012 Apr 19;185(1):32-44.
    doi: 10.1016/j.vetpar.2011.10.012pubmed: 22055611google scholar: lookup
  26. Madeira de Carvalho L.M. Estrongilidose dos equídeos-biologia, patologia, epidemiologia e controlo. In: Tovar J., Reina D., editors. Memoriam Prof. Ignacio Navarrete López-Cózar. Universidad de Extremadura; Cáceres, España: 2006. pp. 277–326.
  27. Couto M, Santos A.S., Laborda J, Nóvoa M, Ferreira L.M., Madeira de Carvalho L.M. Grazing behaviour of Miranda Donkeys in a natural mountain pasture and parasitic level changes. Livest. Sci. 2016;186:16–21.
    doi: 10.1016/j.livsci.2016.01.005google scholar: lookup
  28. Sousa S, Rodriques J, Silva A, Pimentel M, Madeira de Carvalho L.M. Infecção parasitária dos asininos da raça de Miranda em 2005 e 2008. Proceedings of the IV Congresso da Sociedade Portuguesa de Ciências Veterinárias & I Congresso Ibérico de Epidemiologia; Santarém, Portugal. 27–29 November 2008; p. 117.
  29. Sousa S, Madeira de Carvalho L.M. Parasitismo intestinal numa população de asininos com desparasitação regular. Acta Parasitol. Port. 2009;16:220–221.
  30. Nielsen MK. Restrictions of anthelmintic usage: perspectives and potential consequences.. Parasit Vectors 2009 Sep 25;2 Suppl 2(Suppl 2):S7.
    doi: 10.1186/1756-3305-2-S2-S7pmc: PMC2751843pubmed: 19778468google scholar: lookup
  31. Madeira de Carvalho L.M. Epidemiology and Control of Strongylosis in Different Horse Production Systems in Portugal. Ph.D. Thesis. Faculty of Veterinary Medicine, Technical University of Lisbon; Lisbon, Portugal: 2002.
  32. Smith D, Wood S. Donkey nutrition. In: Duncan J., Hadrill D., editors. The Professional Handbook of the Donkey. 4th ed. Compiled for Donkey Sanctuary by Elisabeth D. Svendsen; Wales, UK: 2008. pp. 10–27.
  33. Meirinhos L.M.P. A evolução da Terra de Miranda: Um Estudo com Base nos Sistemas de Informação Geográfica. Master’s Thesis. University of Porto; Porto, Portugal: 2014.
  34. Thienpont D, Rochette F, Vanparijs O.F.J. Diagnóstico de las Helminthiasis por Medio de Examen Coprológico. 2nd ed. Janssen Research Foundation; Beerse, Bélgica: 1986. p. 205.
  35. Lopes A.S., Melo-Franco B, Nunes T, Sousa S, Fabrica P, São-Braz B, Madeira de Carvalho L.M. Distribution, Major Epidemiological Features and Control of Equid Gastrointestinal Parasites in Mainland Portugal. In: Freitas Duarte A., Lopes da Costa L., editors. Advances in Animal Health, Medicine and Production. Springer; Cham, Switzerland: 2020.
    doi: 10.1007/978-3-030-61981-7_19google scholar: lookup
  36. Madeira de Carvalho L.M., Pereira da Fonseca I.M., Afonso-Roque M.M., Marcos M.V.M., Carvalho-Varela M. Estudo das parasitoses dos equídeos do Ribatejo: Parasitoses gastrintestinais e pulmonares. Acta Parasitol. Port. 1993;1:59.
  37. Roberts F.H.S., O’Sullivan P.J. Methods for egg counts and larval cultures for strongyles infesting the gastro-intestinal tract of cattle. Aust. J. Agric. Res. 1950;1:99–102.
    doi: 10.1071/AR9500099google scholar: lookup
  38. Ueno H, Gutierres V.C. Manual para Diagnóstico das Helmintoses de Ruminantes. 2nd ed. Japan International Cooperation Agency; Tóquio, Japão: 1983. p. 176.
  39. Madeira de Carvalho L.M., Fazendeiro M.I., Afonso-Roque M.M. Estudo morfométrico das larvas infectantes (L3) dos estrongilídeos (Nematoda: Strongylidae) dos equídeos. 1. Género Cyathostomum, sensu lato. Acta Parasitol. Port. 2004;11:21–32.
  40. Madeira de Carvalho L.M., Gillespie A.T., Serra P.M., Bernardo F.A., Farrim A.P., Fazendeiro I. Eficácia do fungo nematófago Duddingtonia flagrans no controlo biológico da estrongilidose equina no Ribatejo. Rev. Port. Ciênc. Vet. 2007;102:233–247.
  41. Cernea M, Madeira de Carvalho L.M., Cozma V, Cernea L, Raileanu S, Silberg R, Gut A. Atlas of Diagnosis of Equine Strongylidosis. 1st ed. Academic Pres–Universitatea de Stiinte Agricole si Medicina Veterinara Cluj-Napoca; Cluj, Romania: 2008. p. 120.
  42. Madeira de Carvalho L.M., Cernea M.S., Martins S., Sousa S., Gersão S., Cernea L.C. Comparative study of cyathostomin horse infection in Portugal and Romania based in L3 subpopulations of Cyathostomum sensu latum. Rev. Sci. Parasitol. 2008;9:48–56.
  43. Santos DW, Madeira de Carvalho LM, Molento MB. Identification of third stage larval types of cyathostomins of equids: An improved perspective.. Vet Parasitol 2018 Aug 30;260:49-52.
    doi: 10.1016/j.vetpar.2018.08.007pubmed: 30197013google scholar: lookup
  44. Madeira de Carvalho L.M., Fazendeiro M.I., Afonso-Roque M.M. Estudo morfométrico das larvas infectantes (L3) dos estrongilídeos (Nematoda: Strongylidae) dos equídeos. 3. Conclusões, perspectivas futuras e proposta de chave de identificação de alguns nemátodes gastrintestinais mais comuns dos equídeos em Portugal. Acta Parasitol. Port. 2008;15:57–63.
  45. Nielsen MK, Haaning N, Olsen SN. Strongyle egg shedding consistency in horses on farms using selective therapy in Denmark.. Vet Parasitol 2006 Feb 18;135(3-4):333-5.
    doi: 10.1016/j.vetpar.2005.09.010pubmed: 16226379google scholar: lookup
  46. Becher AM, Mahling M, Nielsen MK, Pfister K. Selective anthelmintic therapy of horses in the Federal states of Bavaria (Germany) and Salzburg (Austria): an investigation into strongyle egg shedding consistency.. Vet Parasitol 2010 Jul 15;171(1-2):116-22.
    doi: 10.1016/j.vetpar.2010.03.001pubmed: 20356680google scholar: lookup
  47. Francisco R, Paz-Silva A, Francisco I, Cortiñas F.J., Miguélez S, Suárez J.L., Cazapal-Monteiro C.F., Arias M.S., Sánchez-Andrade R. Preliminary analysis of the results of selective therapy against strongyles in pasturing horses. J. Equine Vet. Sci. 2012;32:274–280.
    doi: 10.1016/j.jevs.2011.09.074google scholar: lookup
  48. Trawford A.F., Burden F.A. Drug resistant cyathostomins in donkey herds; lessons in management for all equids. J. Equine Vet. Sci. 2012;32:S43.
    doi: 10.1016/j.jevs.2012.08.095google scholar: lookup
  49. Nielsen MK, Pfister K, von Samson-Himmelstjerna G. Selective therapy in equine parasite control--application and limitations.. Vet Parasitol 2014 May 28;202(3-4):95-103.
    doi: 10.1016/j.vetpar.2014.03.020pubmed: 24702770google scholar: lookup
  50. Warnick LD. Daily variability of equine fecal strongyle egg counts.. Cornell Vet 1992 Oct;82(4):453-63.
    pubmed: 1424638
  51. Sréter T, Molnár V, Kassai T. The distribution of nematode egg counts and larval counts in grazing sheep and their implications for parasite control.. Int J Parasitol 1994 Feb;24(1):103-8.
    pubmed: 8021097doi: 10.1016/0020-7519(94)90063-9google scholar: lookup
  52. Greite L. Untersuchungen zur Verbreitung von Strongylus Vulgaris im Rahmen der Selektiven Entwurmung bei Pferden in Süddeutschland. Ph.D. Thesis. Ludwig-Maximilians-Universität; München, Germany: 2013.
  53. Madeira de Carvalho L.M., Sousa S., Cernea M., Cernea L.C., Arias M., Paz-Silva A. Strongyles shed in faeces as a means of monitoring the parasite scenario in horse stud farms. In: Paz Silva A., Arias M.S., Sanchéz-Andrade R., editors. Horses: Breeding, Health Disorders and Effects on Performance & Behavior. Nova Science Publishers; New York, NY, USA: 2014. pp. 93–125.
  54. Monteiro A, Amaro D. Parque Natural do Douro Internacional: Uma área protegida nas Arribas do Douro e Águeda. In: González J., Alberti J., Fernández A., Alonso M., editors. Arribes del Duero: El hogar del Águla Perdicera y de la Cigueña Nega. Volume 190. Consejeria de Meio Ambiente Desarrollo del proyeto LIFE-NAT-B4/3200/97/253; Junta de Castilla y Leon, España: 2000. pp. 159–186.
  55. Moreira N, Aguiar C, Pires J.M. Lameiros e Outros Prados e Pastagens de Elevado Valor Florístico. Pastagens de Montanha. Direcção Geral de Desenvolvimento Rural; Lisbon, Portugal: 2001. p. 47.
  56. Pôças I.M.V.A. Os Lameiros no Contexto das Paisagens de Montanha. Monitorização por Detecção Remota em Diferentes Escalas Espácio-Temporais. Ph.D. Thesis. University of Porto; Porto, Portugal: 2014.
  57. Méndez G.S., Aguiar C. Ecología de los “lameiros” en una aldea del Planalto de Miranda, Trás-os-Montes, Portugal. In Pastos: Fuente Natural de Energía; Proceedings of the 4th Reunião Iberica de Pastagens e Forragens; Zamora-Miranda do Douro, Portugal. 3–6 May 2010; pp. 75–80.
  58. Relf VE, Morgan ER, Hodgkinson JE, Matthews JB. Helminth egg excretion with regard to age, gender and management practices on UK Thoroughbred studs.. Parasitology 2013 Apr;140(5):641-52.
    doi: 10.1017/S0031182012001941pubmed: 23351718google scholar: lookup
  59. Kuz'mina TA. [Strongylids (Nematoda: Strongylidae) of domestic horses in Ukraine: modern state of Fauna and structure of the parasite community].. Parazitologiia 2012 Mar-Apr;46(2):127-38.
    pubmed: 22834350
  60. Matthews J.B., Burden F.A. Common helminth infections of donkeys and their control in temperate regions. Equine Vet. Educ. 2013;25:461–467.
    doi: 10.1111/eve.12018google scholar: lookup
  61. Matthews J.B. An update on cyathostomins: Anthelmintic resistance and worm control. Equine Vet. Educ. 2008;20:552–560.
    doi: 10.2746/095777308X363912google scholar: lookup
  62. Matthews JB. Anthelmintic resistance in equine nematodes.. Int J Parasitol Drugs Drug Resist 2014 Dec;4(3):310-5.
    doi: 10.1016/j.ijpddr.2014.10.003pmc: PMC4266799pubmed: 25516842google scholar: lookup
  63. Schneider S, Pfister K, Becher AM, Scheuerle MC. Strongyle infections and parasitic control strategies in German horses - a risk assessment.. BMC Vet Res 2014 Nov 12;10:262.
    doi: 10.1186/s12917-014-0262-zpmc: PMC4232665pubmed: 25387542google scholar: lookup
  64. . O Burro de Miranda na Gestão de Vegetação Arbustiva-Baixa e de Ecossistemas de Elevado Valor Conservacionista. AEPGA; Miranda do Douro, Portugal: 2015. p. 48. Final Report of EDP Biodiversidade 2010.
  65. Wannas H.Y., Dawood K.H.A., Gassem G.H.A. Prevalence of gastro-intestinal parasites in horses and donkeys in Al-Diwaniyah Governorate. Al Qadisiya J. Vet. Med. Sci. 2012;11:148–155.
    doi: 10.29079/vol11iss1art183google scholar: lookup
  66. Getachew M.A. Endoparasites of Working Donkeys in Ethiopia: Epidemiological Study and Mathematical Modelling. Ph.D. Thesis. University of Glasgow; Glasgow, UK: 2006.
  67. Bell N.J., Morriss C.J., Trawford A.F., Reid S.W.J. The effect of age and sex on the faecal egg count (FEC) in the donkey. Proceedings of the 18th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP); Stresa, Italy. 26–30 August 2001.
  68. Tesfu N, Asrade B, Abebe R, Kasaye S. Prevalence and risk factors of gastrointestinal nematode parasites of horse and donkeys in Hawassa Town, Ethiopia. Vet. Sci. Technol. 2014;5:210.
  69. Mezgebu T, Tafess K, Tamiru F. Prevalence of gastrointestinal parasites of horses and donkeys in and around Gondar Town, Ethiopia. Open J. Vet. Med. 2013;3:267–272.
    doi: 10.4236/ojvm.2013.36043google scholar: lookup
  70. Sheferaw D, Alemu M. Epidemiological study of gastrointestinal helminths of equines in Damot-Gale district, Wolaita zone, Ethiopia.. J Parasit Dis 2015 Jun;39(2):315-20.
    doi: 10.1007/s12639-013-0352-zpmc: PMC4456530pubmed: 26064026google scholar: lookup
  71. Tsegaye B, Chala A. Prevalence of endoparasitic helminths of donkeys in and around Haramaya district, Eastern Ethiopia. J. Vet. Med. Anim. Health. 2015;7:221–224.
  72. Vázquez I.F. Epidemiología y Control de los Principales Parasitismos del Caballo en Galicia. Universidad de Santiago de Compostela; Santiago, Spain: 2010. p. 225.
  73. Ramsey YH, Christley RM, Matthews JB, Hodgkinson JE, McGoldrick J, Love S. Seasonal development of Cyathostominae larvae on pasture in a northern temperate region of the United Kingdom.. Vet Parasitol 2004 Feb 6;119(4):307-18.
    doi: 10.1016/j.vetpar.2003.11.014pubmed: 15154595google scholar: lookup
  74. Lloyd S, Martin SA. Controlled trial on the effects of radionic healing and anthelmintics on faecal egg counts in horses.. Vet Rec 2006 May 27;158(21):734-7.
    doi: 10.1136/vr.158.21.734pubmed: 16731705google scholar: lookup
  75. Kuzmina TA, Kuzmin YI, Kharchenko VA. Field study on the survival, migration and overwintering of infective larvae of horse strongyles on pasture in central Ukraine.. Vet Parasitol 2006 Nov 5;141(3-4):264-72.
    doi: 10.1016/j.vetpar.2006.06.005pubmed: 16860937google scholar: lookup
  76. Baudena MA, Chapman MR, French DD, Klei TR. Seasonal development and survival of equine cyathostome larvae on pasture in south Louisiana.. Vet Parasitol 2000 Feb 29;88(1-2):51-60.
    doi: 10.1016/S0304-4017(99)00198-3pubmed: 10681022google scholar: lookup
  77. Elsener J, Villeneuve A. Comparative long-term efficacy of ivermectin and moxidectin over winter in Canadian horses treated at removal from pastures for winter housing.. Can Vet J 2009 May;50(5):486-90.
    pmc: PMC2671870pubmed: 19436633
  78. Giles CJ, Urquhart KA, Longstaffe JA. Larval cyathostomiasis (immature trichonema-induced enteropathy): a report of 15 clinical cases.. Equine Vet J 1985 May;17(3):196-201.
    doi: 10.1111/j.2042-3306.1985.tb02469.xpubmed: 4076127google scholar: lookup
  79. Reinemeyer CR. Small strongyles. Recent advances.. Vet Clin North Am Equine Pract 1986 Aug;2(2):281-312.
    doi: 10.1016/S0749-0739(17)30717-4pubmed: 3527374google scholar: lookup
  80. Preto E, Sá A. Património Geológico nos Parques Naturais do Douro Internacional e Arribas del Duero (Ramo Norte). Porto Editora; Porto, Portugal: 2007. p. 28.
  81. Mattioli RC, Zinsstag J, Pfister K. Frequency of trypanosomosis and gastrointestinal parasites in draught donkeys in The Gambia in relation to animal husbandry.. Trop Anim Health Prod 1994 May;26(2):102-8.
    doi: 10.1007/BF02239909pubmed: 7941024google scholar: lookup
  82. Wells D, Krecek RC, Wells M, Guthrie AJ, Lourens JC. Helminth levels of working donkeys kept under different management systems in the Moretele 1 district of the North-West Province, South Africa.. Vet Parasitol 1998 Jun 15;77(2-3):163-77.
    doi: 10.1016/S0304-4017(98)00105-8pubmed: 9746286google scholar: lookup
  83. Gebread F. Diseases and health problems of donkeys abroad. In: Svendsen E.D., editor. The Professional Handbook of the Donkey. 3rd ed. Whittet Books; London, UK: 1997. p. 400.
  84. Collobert-Laugier C, Hoste H, Sevin C, Dorchies P. Prevalence, abundance and site distribution of equine small strongyles in Normandy, France.. Vet Parasitol 2002 Dec 11;110(1-2):77-83.
    doi: 10.1016/S0304-4017(02)00328-Xpubmed: 12446091google scholar: lookup
  85. Matthee S, Dreyer FH, Hoffmann WA, van Niekerk FE. An introductory survey of helminth control practices in south africa and anthelmintic resistance on Thoroughbred stud farms in the Western Cape Province.. J S Afr Vet Assoc 2002 Dec;73(4):195-200.
    doi: 10.4102/jsava.v73i4.586pubmed: 12665133google scholar: lookup
  86. Kuzmina T, Kharchenko V, Zvegintsova N. Comparative study of the intestinal strongylid communities of equidae in the Askania-Nova biosphere reserve, Ukraine. J. Helminthol. 2007;44:62–69.
    doi: 10.2478/s11687-007-0005-9google scholar: lookup
  87. Kuzmina T.A., Kuzmin Y.I. The community of strongylids (Nematoda, Strongylida) of working donkeys (Equus asinus) in Ukraine. Vestn. Zool. 2008;42:18–23.
    doi: 10.2478/v10058-008-0004-4google scholar: lookup
  88. Getachew M, Trawford A, Feseha G, Reid SW. Gastrointestinal parasites of working donkeys of Ethiopia.. Trop Anim Health Prod 2010 Jan;42(1):27-33.
    doi: 10.1007/s11250-009-9381-0pubmed: 19548106google scholar: lookup
  89. Ferreira G.M.S., Dutra F.A.F., Filho E.F.A., Santos A.C.G. Parasitismo gastrintestinal e hematologia em equinos e asininos da mesorregião da aglomeração urbana, São Luís, Maranhão. Arch. Vet. Sci. 2014;19:22–30.
    doi: 10.5380/avs.v19i2.32898google scholar: lookup
  90. Menzel M.A. Selektive Entwurmung der Pferde in Einer Oberbayrischen Pferdepraxis: Einführung Sowie Wissenschaftliche und Betriebswirtschaftliche Analyse. Ph.D. Thesis. Ludwig-Maximilians-Universität; München, Germany: 2013.
  91. Matthee S, Krecek RC, Milne SA, Boshoff M, Guthrie AJ. Impact of management interventions on helminth levels, and body and blood measurements in working donkeys in South Africa.. Vet Parasitol 2002 Jul 29;107(1-2):103-13.
    doi: 10.1016/S0304-4017(02)00113-9pubmed: 12072218google scholar: lookup
  92. Osterman Lind E, Eysker M, Nilsson O, Uggla A, Höglund J. Expulsion of small strongyle nematodes (cyathostomin spp) following deworming of horses on a stud farm in Sweden.. Vet Parasitol 2003 Aug 14;115(4):289-99.
    doi: 10.1016/S0304-4017(03)00200-0pubmed: 12944042google scholar: lookup
  93. Peregrine AS, McEwen B, Bienzle D, Koch TG, Weese JS. Larval cyathostominosis in horses in Ontario: an emerging disease?. Can Vet J 2006 Jan;47(1):80-2.
    pmc: PMC1316126pubmed: 16536234
  94. Love S. Treatment and prevention of intestinal parasite-associated disease.. Vet Clin North Am Equine Pract 2003 Dec;19(3):791-806.
    doi: 10.1016/j.cveq.2003.08.003pubmed: 14740770google scholar: lookup
  95. Becher AM, Pfister K. [The efficacy of anthelmintic drugs against horse strongyles in the area of Salzburg and preliminary results of selective anthelmintic treatment].. Wien Klin Wochenschr 2010 Oct;122 Suppl 3:71-5.
    doi: 10.1007/s00508-010-1441-ypubmed: 20924696google scholar: lookup
  96. Madeira de Carvalho L.M., Gomes L., Cernea M., Cernea C., Santos C.A., Bernardes N., Rosário M.A., Soares M.J., Fazendeiro M.I. Parasitismo gastrintestinal e seu controlo em asininos e híbridos estabulados. Rev. Port. Ciênc. Vet. 2007;102:225–231.
  97. Ibrahim N, Berhanu T, Deressa B, Tolosa T. Survey of prevalence of helminth parasites of donkeys in and around Hawassa Town, Southern Ethiopia. Glob. Vet. 2011;6:223–227.
  98. Filho E.F.A., Santos A.C.G., Dutra F.A.F., Ferreira G.M.S., Guerra R.M.C. Avaliação do parasitismo gastrintestinal de asininos da ilha de São Luís, Estado do Maranhão. Encicl. Biosf. Cent. Cient. Conhecer. 2013;9:393–399.
  99. Matto TN, Bharkad GP, Bhat SA. Prevalence of gastrointestinal helminth parasites of equids from organized farms of Mumbai and Pune.. J Parasit Dis 2015 Jun;39(2):179-85.
    doi: 10.1007/s12639-013-0315-4pmc: PMC4456555pubmed: 26063996google scholar: lookup
  100. Borji H, Moosavi Z, Ahmadi F. Cranial Mesenteric Arterial Obstruction Due To Strongylus vulgaris Larvae in a Donkey (Equus asinus).. Iran J Parasitol 2014 Sep;9(3):441-4.
    pmc: PMC4316578pubmed: 25678931
  101. Pandey VS, Eysker M. Parasites of the stomach in donkeys of the highveld of Zimbabwe.. Vet Q 1988 Oct;10(4):246-8.
    doi: 10.1080/01652176.1988.9694180pubmed: 2975414google scholar: lookup
  102. Khallaayoune K. Benefit of a strategic deworming programme in working donkeys in Marocco. In: Fielding D., Pearson R.A., editors. Donkeys, Mules and Horses in Tropical Agricultural Development. Centre for Tropical Veterinary Medicine, University of Edinburgh; Edinburgh, UK: 1991. pp. 174–180.
  103. Pilo C, Altea A, Pirino S, Nicolussi P, Varcasia A, Genchi M, Scala A. Strongylus vulgaris (Looss, 1900) in horses in Italy: is it still a problem?. Vet Parasitol 2012 Mar 23;184(2-4):161-7.
    doi: 10.1016/j.vetpar.2011.09.016pubmed: 21962966google scholar: lookup
  104. Studzińska MB, Tomczuk K, Demkowska-Kutrzepa M, Szczepaniak K. The Strongylidae belonging to Strongylus genus in horses from southeastern Poland.. Parasitol Res 2012 Oct;111(4):1417-21.
    doi: 10.1007/s00436-012-3087-3pmc: PMC3447134pubmed: 22961235google scholar: lookup
  105. Hertzberg H, Schwarzwald CC, Grimm F, Frey CF, Gottstein B, Gerber V. [Helminth control in the adult horse: the need for a re-orientation].. Schweiz Arch Tierheilkd 2014 Feb;156(2):61-70.
    doi: 10.1024/0036-7281/a000552pubmed: 24463320google scholar: lookup
  106. Getachew AM, Innocent GT, Trawford AF, Feseha G, Reid SJ, Love S. Equine parascarosis under the tropical weather conditions of Ethiopia: a coprological and postmortem study.. Vet Rec 2008 Feb 9;162(6):177-80.
    doi: 10.1136/vr.162.6.177pubmed: 18263917google scholar: lookup
  107. Trawford A, Mulugeta G. Parasites. In: Duncan J., Hadrill D., editors. The Professional Handbook of the Donkey. 4th ed. Compiled for Donkey Sanctuary by Elisabeth D. Svendsen; Wales, UK: 2008. pp. 82–110.

Citations

This article has been cited 6 times.
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    doi: 10.3390/ani13142331pubmed: 37508108google scholar: lookup
  2. Silva V, Alfarela C, Caniça M, Manageiro V, Nóvoa M, Leiva B, Kress M, Capelo JL, Poeta P, Igrejas G. A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers. Antibiotics (Basel) 2022 Mar 10;11(3).
    doi: 10.3390/antibiotics11030374pubmed: 35326837google scholar: lookup
  3. Costa C, Sousa AP, Silva G, Queiroga F, Martins Â, Andrade D, Silvestre-Ferreira AC. Analytical Validation of Two Point-of-Care Assays for Hematological Analysis in the Miranda Donkey. Vet Sci 2024 Sep 22;11(9).
    doi: 10.3390/vetsci11090450pubmed: 39330829google scholar: lookup
  4. Silva G, Silvestre-Ferreira AC, Leiva B, Queiroga FL. Serum Biochemistry Parameters of the Endangered Miranda's Donkey Breed: Reference Intervals and the Influence of Gender and Age. Animals (Basel) 2024 Mar 5;14(5).
    doi: 10.3390/ani14050805pubmed: 38473190google scholar: lookup
  5. Simões J, Sales Luís JP, Madeira de Carvalho L, Tilley P. Severely Asthmatic Horses Residing in a Mediterranean Climate Shed a Significantly Lower Number of Parasite Eggs Compared to Healthy Farm Mates. Animals (Basel) 2023 Sep 15;13(18).
    doi: 10.3390/ani13182928pubmed: 37760328google scholar: lookup
  6. Buono F, Veneziano V, Veronesi F, Molento MB. Horse and donkey parasitology: differences and analogies for a correct diagnostic and management of major helminth infections. Parasitology 2023 Oct;150(12):1119-1138.
    doi: 10.1017/S0031182023000525pubmed: 37221816google scholar: lookup
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