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Histochemistry and cell biology2010; 134(6); 545-554; doi: 10.1007/s00418-010-0760-4

Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells.

Abstract: Adipose-derived stromal cells (ADSCs) are multipotent cells which, in the presence of appropriate stimuli, can differentiate into various lineages such as the osteogenic, adipogenic and chondrogenic. In this study, we investigated the effect of transforming growth factor beta 1 (TGF-β1) in comparison to hydrolyzed fish collagen in terms of the chondrogenic differentiation potential of ADSCs. ADSCs were isolated from subcutaneous fat of horses by liposuction. Chondrogenesis was investigated using a pellet culture system. The differentiation medium was either supplemented with TGF-β1 (5 ng/ml) or fish collagen (0.5 mg/ml) for a 3 week period. After the 3 weeks in vitro differentiation, RT-PCR and histological staining for proteoglycan synthesis and type II collagen were performed to evaluate the degree of chondrogenic differentiation and the formation of cartilaginous extracellular matrix (ECM). The differentiation of ADSCs induced by TGF-β1 showed a high expression of glycosaminoglycan (GAG). Histological analysis of cultures stimulated by hydrolyzed fish collagen demonstrated an even higher GAG expression than cultures stimulated under standard conditions by TGF-β1. The expression of cartilage-specific type II collagen and Sox9 was about the same in both stimulated cultures. In this study, chondrogenesis was as effectively induced by hydrolyzed fish collagen as it was successfully induced by TGF-β1. These findings demonstrated that hydrolyzed fish collagen alone has the potential to induce and maintain ADSCs-derived chondrogenesis. These results support the application of ADSCs in equine veterinary tissue engineering, especially for cartilage repair.
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Publication Date: 2010-11-14 PubMed ID: 21076963DOI: 10.1007/s00418-010-0760-4Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the impact of hydrolyzed fish collagen and transforming growth factor beta 1 (TGF-β1) on the transformation of equine adipose-derived stromal cells (ADSCs) into cartilage. The results show that hydrolyzed fish collagen can successfully stimulate these cells to differentiate into cartilage, potentially serving as a new approach in equine veterinary tissue engineering, particularly in cartilage repair.

Methodology

  • ADSCs, multipotent cells with the capability to differentiate into various types of cells like osteogenic, adipogenic and chondrogenic cells, were extracted from the subcutaneous fat of horses through liposuction.
  • The differentiation process was tested using a pellet culture system wherein the differentiation medium was supplemented with either TGF-β1 or hydrolyzed fish collagen for three weeks.

Investigation of Chondrogenesis

  • After the three week differentiation process, examinations were performed through RT-PCR and histological staining which reflected proteoglycan synthesis and type II collagen. These tests suggest the level of chondrogenic differentiation and the formation of the cartilaginous extracellular matrix (ECM).
  • TGF-β1 induced-ADSC differentiation demonstrated high glycosaminoglycan (GAG) expression. Conversely, hydrolyzed fish collagen stimulated cultures exhibited an even higher GAG expression compared to those induced by TGF-β1.

Comparing the Impact of Hydrolyzed Fish Collagen and TGF-β1

  • The expression of cartilage-specific type II collagen and Sox9 was observed to be almost the same in cultures stimulated by either hydrolyzed fish collagen or TGF-β1.
  • Both hydrolyzed fish collagen and TGF-β1 were able to effectively induce chondrogenesis. Nevertheless, this study emphasizes that hydrolyzed fish collagen alone has the capability to instigate and uphold chondrogenesis derived from ADSCs.

Implications of the Study

  • This study presents the potential application of hydrolyzed fish collagen in tissue engineering, particularly in repairing cartilage in equine veterinary medicine.

Cite This Article

APA
Raabe O, Reich C, Wenisch S, Hild A, Burg-Roderfeld M, Siebert HC, Arnhold S. (2010). Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells. Histochem Cell Biol, 134(6), 545-554. https://doi.org/10.1007/s00418-010-0760-4

Publication

Histochemistry and cell biology
ISSN: 1432-119X
NlmUniqueID: 9506663
Country: Germany
Language: English
Volume: 134
Issue: 6
Pages: 545-554

Researcher Affiliations

Raabe, O
  • Institute of Veterinary Anatomy, Histology, and Embryology, Justus-Liebig University of Giessen, Giessen, Germany. oksana.raabe@vetmed.uni-giessen.de
Reich, C
    Wenisch, S
      Hild, A
        Burg-Roderfeld, M
          Siebert, H-C
            Arnhold, S

              MeSH Terms

              • Adipose Tissue / cytology
              • Animals
              • Cell Differentiation / drug effects
              • Chondrocytes / ultrastructure
              • Chondrogenesis / drug effects
              • Collagen / pharmacology
              • Collagen Type II / biosynthesis
              • Collagen Type II / ultrastructure
              • Extracellular Matrix / metabolism
              • Fishes
              • Horses
              • Protein Hydrolysates / pharmacology
              • Stromal Cells / metabolism
              • Transforming Growth Factor beta1 / pharmacology

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