FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Cell Biol Int Rep (2010) / Isolation and characterization of equine amnion mesenchymal ...
Cell biology international reports2011; 18(1); e00011; doi: 10.1042/CBR20110004

Isolation and characterization of equine amnion mesenchymal stem cells.

Abstract: The amnion is a particular tissue whose cells show features of multipotent stem cells proposed for use in cellular therapy and regenerative medicine. From equine amnion collected after the foal birth we have isolated MSCs (mesenchymal stem cells), namely EAMSCs (equine amnion mesenchymal stem cells), from the mesoblastic layer. The cells were grown in α-MEM (α-modified minimum essential medium) and the effect of EGF (epidermal growth factor) supplementation was evaluated. To assess the growth kinetic of EAMSCs we have taken into account some parameters [PD (population doubling), fold increase and DT (doubling time)]. The differentiation in chondrogenic, adipogenic and osteogenic types of cells and their epitope expression by a cytofluorimetric study have been reported. EGF supplementation of the culture medium resulted in a significant increase in PD growth parameter and in the formation of bone nodules for the osteogenic differentiation. By immunohistochemistry the amnion tissue shows a positivity for the c-Kit (cluster tyrosine-protein kinase), CD105 and Oct-4 (octamer-binding transcription factor 4) antigens that confirmed the presence of MSCs with embryonic phenotype.
Get Full Text
Publication Date: 2011-09-13 PubMed ID: 23124164PubMed Central: PMC3475441DOI: 10.1042/CBR20110004Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research pertains to the extraction and study of equine amnion mesenchymal stem cells (EAMSCs) from horse amnion tissue, with an aim to understand its potential use in therapy and regenerative medicine.

Method of Isolation

  • The researchers collected equine amnion, a specific type of tissue found in horses, after the birth of a foal.
  • The researchers specifically extracted multipotent mesenchymal stem cells (MSCs) from the mesoblastic layer of the amnion. These MSCs are termed EAMSCs.
  • The cultured EAMSCs were grown in α-MEM (α-modified minimum essential medium) for further examination.

Impact of EGF Supplementation

  • The study also evaluated the effect of supplementation with epidermal growth factor (EGF) on EAMSCs. It was found that EGF supplementation significantly increased the population doubling (PD) growth parameter of the cells.
  • The use of EGF also led to an increase in bone nodule formation, an indicator of osteogenic (bone-forming) differentiation.

Study of Growth Kinetics

  • The researchers studied the growth kinetics of EAMSCs by considering parameters like population doubling (PD), fold increase, and doubling time (DT).

Differentiation Capacity

  • EAMSCs were assessed for their ability to differentiate (transform) into chondrogenic (cartilage-forming), adipogenic (fat-storing) and osteogenic (bone-forming) types of cells.
  • This differentiation capacity of stem cells is crucial for their potential use in regenerative medicine and cellular therapy.

Cytofluorimetric Study & Immunohistochemistry

  • A cytofluorimetric study was used to report the epitope expression (surface markers) of the cells, giving further insight into their identity and function.
  • By immunohistochemistry, the amnion tissue was confirmed to contain MSCs with an embryonic phenotype, as evidenced by the positive marks for the antigens c-Kit (a type of protein kinase), CD105, and Oct-4 (a transcription factor related to stem cell regulation).

Overall, this research provides valuable information about EAMSCs, which could be potentially used for therapeutic purposes such as the treatment of various degenerative conditions or injuries.

Cite This Article

APA
Coli A, Nocchi F, Lamanna R, Iorio M, Lapi S, Urciuoli P, Scatena F, Giannessi E, Stornelli MR, Passeri S. (2011). Isolation and characterization of equine amnion mesenchymal stem cells. Cell Biol Int Rep (2010), 18(1), e00011. https://doi.org/10.1042/CBR20110004

Publication

Cell biology international reports
ISSN: 2041-5346
NlmUniqueID: 101574535
Country: England
Language: English
Volume: 18
Issue: 1
Pages: e00011
PII: e00011

Researcher Affiliations

Coli, Alessandra
  • Department of Animal Pathology, University of Pisa, Pisa, Italy.
Nocchi, Francesca
    Lamanna, Roberta
      Iorio, Mariacarla
        Lapi, Simone
          Urciuoli, Patrizia
            Scatena, Fabrizio
              Giannessi, Elisabetta
                Stornelli, Maria Rita
                  Passeri, Simona

                    References

                    This article includes 21 references
                    1. Arnhold SJ, Goletz I, Klein H, Stumpf G, Beluche LA, Rohde C, Addicks K, Litzke LF. Isolation and characterization of bone marrow-derived equine mesenchymal stem cells.. Am J Vet Res 2007 Oct;68(10):1095-105.
                      pubmed: 17916017doi: 10.2460/ajvr.68.10.1095google scholar: lookup
                    2. Fong CY, Richards M, Manasi N, Biswas A, Bongso A. Comparative growth behaviour and characterization of stem cells from human Wharton's jelly.. Reprod Biomed Online 2007 Dec;15(6):708-18.
                      pubmed: 18062871doi: 10.1016/s1472-6483(10)60539-1google scholar: lookup
                    3. Hoynowski SM, Fry MM, Gardner BM, Leming MT, Tucker JR, Black L, Sand T, Mitchell KE. Characterization and differentiation of equine umbilical cord-derived matrix cells.. Biochem Biophys Res Commun 2007 Oct 19;362(2):347-53.
                      pubmed: 17719011doi: 10.1016/j.bbrc.2007.07.182google scholar: lookup
                    4. Insausti CL, Blanquer M, Bleda P, Iniesta P, Majado MJ, Castellanos G, Moraleda JM. The amniotic membrane as a source of stem cells.. Histol Histopathol 2010 Jan;25(1):91-8.
                      pubmed: 19924645doi: 10.14670/HH-25.91google scholar: lookup
                    5. Kim J, Lee Y, Kim H, Hwang KJ, Kwon HC, Kim SK, Cho DJ, Kang SG, You J. Human amniotic fluid-derived stem cells have characteristics of multipotent stem cells.. Cell Prolif 2007 Feb;40(1):75-90.
                      pmc: PMC6496664pubmed: 17227297doi: 10.1111/j.1365-2184.2007.00414.xgoogle scholar: lookup
                    6. Kisiday JD, Kopesky PW, Evans CH, Grodzinsky AJ, McIlwraith CW, Frisbie DD. Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures.. J Orthop Res 2008 Mar;26(3):322-31.
                      pubmed: 17960654doi: 10.1002/jor.20508google scholar: lookup
                    7. Kobayashi M, Yakuwa T, Sasaki K, Sato K, Kikuchi A, Kamo I, Yokoyama Y, Sakuragawa N. Multilineage potential of side population cells from human amnion mesenchymal layer.. Cell Transplant 2008;17(3):291-301.
                      pubmed: 18522232doi: 10.3727/096368908784153904google scholar: lookup
                    8. Koch TG, Heerkens T, Thomsen PD, Betts DH. Isolation of mesenchymal stem cells from equine umbilical cord blood.. BMC Biotechnol 2007 May 30;7:26.
                      pmc: PMC1904213pubmed: 17537254doi: 10.1186/1472-6750-7-26google scholar: lookup
                    9. Koerner J, Nesic D, Romero JD, Brehm W, Mainil-Varlet P, Grogan SP. Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells.. Stem Cells 2006 Jun;24(6):1613-9.
                      pubmed: 16769763doi: 10.1634/stemcells.2005-0264google scholar: lookup
                    10. Krampera M, Pasini A, Rigo A, Scupoli MT, Tecchio C, Malpeli G, Scarpa A, Dazzi F, Pizzolo G, Vinante F. HB-EGF/HER-1 signaling in bone marrow mesenchymal stem cells: inducing cell expansion and reversibly preventing multilineage differentiation.. Blood 2005 Jul 1;106(1):59-66.
                      pubmed: 15755902doi: 10.1182/blood-2004-09-3645google scholar: lookup
                    11. Meirelles Lda S, Nardi NB. Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization.. Br J Haematol 2003 Nov;123(4):702-11.
                      pubmed: 14616976doi: 10.1046/j.1365-2141.2003.04669.xgoogle scholar: lookup
                    12. Miki T, Strom SC. Amnion-derived pluripotent/multipotent stem cells.. Stem Cell Rev 2006;2(2):133-42.
                      pubmed: 17237552doi: 10.1007/s12015-006-0020-0google scholar: lookup
                    13. Passeri S, Nocchi F, Lamanna R, Lapi S, Miragliotta V, Giannessi E, Abramo F, Stornelli MR, Matarazzo M, Plenteda D, Urciuoli P, Scatena F, Coli A. Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs).. Cell Biol Int 2009 Jan;33(1):100-5.
                      pubmed: 18996215doi: 10.1016/j.cellbi.2008.10.012google scholar: lookup
                    14. Qiao C, Xu W, Zhu W, Hu J, Qian H, Yin Q, Jiang R, Yan Y, Mao F, Yang H, Wang X, Chen Y. Human mesenchymal stem cells isolated from the umbilical cord.. Cell Biol Int 2008 Jan;32(1):8-15.
                      pubmed: 17904875doi: 10.1016/j.cellbi.2007.08.002google scholar: lookup
                    15. Reed SA, Johnson SE. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.. J Cell Physiol 2008 May;215(2):329-36.
                      pubmed: 17929245doi: 10.1002/jcp.21312google scholar: lookup
                    16. Secco M, Zucconi E, Vieira NM, Fogaça LL, Cerqueira A, Carvalho MD, Jazedje T, Okamoto OK, Muotri AR, Zatz M. Multipotent stem cells from umbilical cord: cord is richer than blood!. Stem Cells 2008 Jan;26(1):146-50.
                      pubmed: 17932423doi: 10.1634/stemcells.2007-0381google scholar: lookup
                    17. Tamama K, Fan VH, Griffith LG, Blair HC, Wells A. Epidermal growth factor as a candidate for ex vivo expansion of bone marrow-derived mesenchymal stem cells.. Stem Cells 2006 Mar;24(3):686-95.
                      pubmed: 16150920doi: 10.1634/stemcells.2005-0176google scholar: lookup
                    18. Toda A, Okabe M, Yoshida T, Nikaido T. The potential of amniotic membrane/amnion-derived cells for regeneration of various tissues.. J Pharmacol Sci 2007 Nov;105(3):215-28.
                      pubmed: 17986813doi: 10.1254/jphs.cr0070034google scholar: lookup
                    19. Vidal MA, Kilroy GE, Johnson JR, Lopez MJ, Moore RM, Gimble JM. Cell growth characteristics and differentiation frequency of adherent equine bone marrow-derived mesenchymal stromal cells: adipogenic and osteogenic capacity.. Vet Surg 2006 Oct;35(7):601-10.
                      pubmed: 17026544doi: 10.1111/j.1532-950X.2006.00197.xgoogle scholar: lookup
                    20. Vidal MA, Kilroy GE, Lopez MJ, Johnson JR, Moore RM, Gimble JM. Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells.. Vet Surg 2007 Oct;36(7):613-22.
                      pubmed: 17894587doi: 10.1111/j.1532-950X.2007.00313.xgoogle scholar: lookup
                    21. Wang HS, Hung SC, Peng ST, Huang CC, Wei HM, Guo YJ, Fu YS, Lai MC, Chen CC. Mesenchymal stem cells in the Wharton's jelly of the human umbilical cord.. Stem Cells 2004;22(7):1330-7.
                      pubmed: 15579650doi: 10.1634/stemcells.2004-0013google scholar: lookup

                    Citations

                    This article has been cited 5 times.
                    1. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance. Cell Transplant 2018 Jan;27(1):93-116.
                      doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
                    2. Borghesi J, Mario LC, Carreira AC, Miglino MA, Favaron PO. Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells. Stem Cell Res Ther 2017 Feb 7;8(1):27.
                      doi: 10.1186/s13287-016-0468-zpubmed: 28173846google scholar: lookup
                    3. Sadeesh Em, Shah F, Yadav PS. Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells. Cytotechnology 2016 Oct;68(5):1827-48.
                      doi: 10.1007/s10616-015-9936-zpubmed: 26660476google scholar: lookup
                    4. Ghosh K, Kumar R, Singh J, Gahlawat SK, Kumar D, Selokar NL, Yadav SP, Gulati BR, Yadav PS. Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro. In Vitro Cell Dev Biol Anim 2015 Oct;51(9):915-21.
                      doi: 10.1007/s11626-015-9920-0pubmed: 26019121google scholar: lookup
                    5. Li D, Wang N, Zhang L, Hanyu Z, Xueyuan B, Fu B, Shaoyuan C, Zhang W, Xuefeng S, Li R, Chen X. Mesenchymal stem cells protect podocytes from apoptosis induced by high glucose via secretion of epithelial growth factor. Stem Cell Res Ther 2013;4(5):103.
                      doi: 10.1186/scrt314pubmed: 24004644google scholar: lookup
                    Subscribe to our newsletter
                    Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.