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BMC veterinary research2011; 7; 42; doi: 10.1186/1746-6148-7-42

Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.

Abstract: The equine periodontium provides tooth support and lifelong tooth eruption on a remarkable scale. These functions require continuous tissue remodeling. It is assumed that multipotent mesenchymal stromal cells (MSC) reside in the periodontal ligament (PDL) and play a crucial role in regulating physiological periodontal tissue regeneration. The aim of this study was to isolate and characterize equine periodontal MSC. Tissue samples were obtained from four healthy horses. Primary cell populations were harvested and cultured from the gingiva, from three horizontal levels of the PDL (apical, midtooth and subgingival) and for comparison purposes from the subcutis (masseteric region). Colony-forming cells were grown on uncoated culture dishes and typical in vitro characteristics of non-human MSC, i.e. self-renewal capacity, population doubling time, expression of stemness markers and trilineage differentiation were analyzed. Results: Colony-forming cell populations from all locations showed expression of the stemness markers CD90 and CD105. In vitro self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFU-F) assays. CFU-efficiency was highest in cell populations from the apical and from the mid-tooth PDL. Population doubling time was highest in subcutaneous cells. All investigated cell populations possessed trilineage differentiation potential into osteogenic, adipogenic and chondrogenic lineages. Conclusions: Due to the demonstrated in vitro characteristics cells were referred to as equine subcutaneous MSC (eSc-MSC), equine gingival MSC (eG-MSC) and equine periodontal MSC (eP-MSC). According to different PDL levels, eP-MSC were further specified as eP-MSC from the apical PDL (eP-MSCap), eP-MSC from the mid-tooth PDL (eP-MSCm) and eP-MSC from the subgingival PDL (eP-MSCsg). Considering current concepts of cell-based regenerative therapies in horses, eP-MSC might be promising candidates for future clinical applications in equine orthopedic and periodontal diseases.
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Publication Date: 2011-08-02 PubMed ID: 21810270PubMed Central: PMC3161857DOI: 10.1186/1746-6148-7-42Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article “Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse” explores the role of particular cells in the horse’s periodontal tissue regeneration process and identifies these cells as potential candidates for developing regenerative therapies for equine orthopedic and periodontal diseases.

Research Objective and Methodology

  • The primary aim of the study was to isolate and characterize multipotent mesenchymal stromal cells (MSC), which are assumed to play a significant role in the regulation of physiological periodontal tissue regeneration in horses.
  • The researchers conducted the study by obtaining tissue samples from four healthy horses. The tissue samples were harvested and cultured from the gingiva, from three horizontal levels of their periodontal ligament (apical, midtooth, and subgingival), and from the subcutis (masseteric region) for comparison.
  • The study focused on investigating various in vitro characteristics of non-human MSC, such as self-renewal capacity, population doubling time, expression of stemness markers, and trilineage differentiation. These characteristics were analyzed by growing colony-forming cells on uncoated culture dishes.

Major Findings of the Research

  • The research revealed that the colony-forming cell populations from all the locations showed the expression of stemness markers CD90 and CD105. This suggested that these cells have the innate ability to generate different cell types.
  • The self-renewal capacity of the cells was confirmed through colony-forming unit fibroblast (CFU-F) assays. The highest CFU-efficiency was observed in cell populations from the apical and mid-tooth periodontal ligament.
  • The research demonstrated that all the investigated cell populations possessed the ability for trilineage differentiation potential into osteogenic, adipogenic, and chondrogenic lineages. This further validated their multipotent nature.
  • The periodontal ligament cells were finally classified as equine subcutaneous MSC (eSc-MSC), equine gingival MSC (eG-MSC), and equine periodontal MSC (eP-MSC); with additional sub-classifications for eP-MSC based on their level in the periodontal ligament.

Implications of the Research

  • The results of the study indicated that the cells identified (eSc-MSC, eG-MSC, and eP-MSC) have the potential to be used in regenerative therapies for equine orthopedic and periodontal diseases.
  • The understanding of the role of these cells in periodontal tissue regeneration paves the way for advancing cell-based therapeutic strategies in veterinary medicine, specifically for horses.

Cite This Article

APA
Mensing N, Gasse H, Hambruch N, Haeger JD, Pfarrer C, Staszyk C. (2011). Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse. BMC Vet Res, 7, 42. https://doi.org/10.1186/1746-6148-7-42

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 7
Pages: 42

Researcher Affiliations

Mensing, Niels
  • Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany.
Gasse, Hagen
    Hambruch, Nina
      Haeger, Jan-Dirk
        Pfarrer, Christiane
          Staszyk, Carsten

            MeSH Terms

            • Animals
            • Cell Culture Techniques / methods
            • Cell Culture Techniques / veterinary
            • Cell Differentiation / physiology
            • Chondrocytes / cytology
            • Female
            • Horses / anatomy & histology
            • Male
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / ultrastructure
            • Microscopy, Phase-Contrast / veterinary
            • Periodontal Ligament / cytology
            • RNA, Messenger / chemistry
            • RNA, Messenger / genetics
            • Reverse Transcriptase Polymerase Chain Reaction / veterinary

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