Isolation of equine peripheral blood stem cells from a Japanese native horse.
Abstract: The sizes of Japanese native horses have drastically decreased, and protection of these populations is important for Japanese horse culture. Social trials as well as scientific attempts are necessary for maintaining the breed. Mesenchymal stem cells (MSCs) have potential as a cell source for various cell therapies. However, there have been no reports on MSCs of Japanese native horses. We aimed to isolate and characterize MSCs from a Japanese native horse, the Noma horse. Plastic-adherent and self-replicating cells were isolated from a Noma horse's peripheral blood (PB). The isolated cells had trilineage potential and a surface antigen of mesenchymal cells, so they fulfilled the minimal criteria of MSCs. Therefore, PB can be one source of MSCs for Japanese native horses.
Publication Date: 2017-12-16 PubMed ID: 29270073PubMed Central: PMC5735313DOI: 10.1294/jes.28.153Google Scholar: Lookup
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- Journal Article
Summary
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This study focuses on the isolation and analysis of stem cells from the peripheral blood of a native Japanese horse, the Noma horse. This is part of efforts to preserve the breed, whose numbers have significantly declined, and to examine the cells’ potential for various cell therapies.
Objective and Background
- Research aims at isolating and characterizing Mesenchymal stem cells (MSCs) from a Japanese native horse, specifically the Noma horse breed. This breed has been rapidly declining in numbers, thus preservation is crucial.
- Mesenchymal stem cells have been identified as having potential as a cell source for various cell therapies due to their regenerative properties. They have not been studied in Japanese native horses previously.
Methods and Results
- Scientists isolated plastic-adherent and self-replicating cells from the peripheral blood (PB) of a Noma horse. Peripheral blood stem cells are blood cells produced in the bone marrow and released into the bloodstream where they can be collected.
- These isolated cells were found to have trilineage potential meaning they have the ability to differentiate into three types of cells, and a surface antigen characteristic of mesenchymal cells, suggesting that they fulfil the minimal criteria of MSCs. An antigen is a foreign substance which induces an immune response in the body, especially the production of antibodies.
Conclusion
- The study concludes that peripheral blood can serve as a source of MSCs for Japanese native horses. This finding opens potential avenues for preserving the Noma horse breed and for future therapeutic strategies based on MSCs.
Cite This Article
APA
Ishikawa S, Horinouchi C, Mizoguchi R, Senokuchi A, Kamikakimoto R, Murata D, Hatazoe T, Tozaki T, Misumi K, Hobo S.
(2017).
Isolation of equine peripheral blood stem cells from a Japanese native horse.
J Equine Sci, 28(4), 153-158.
https://doi.org/10.1294/jes.28.153 Publication
Researcher Affiliations
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
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