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Home / Equine Research Bank / Pathogens / Low Prevalence of Enzootic Equine Influenza Virus among Hors...
Pathogens (Basel, Switzerland)2017; 6(4); 61; doi: 10.3390/pathogens6040061

Low Prevalence of Enzootic Equine Influenza Virus among Horses in Mongolia.

Abstract: Horses are critically important for Mongolian herders' livelihoods, providing transportation and food products, and playing important cultural roles. Equine influenza virus (EIV) epizootics have been frequent among Mongolia's horses, with five occurring since 1970. We sought to estimate the prevalence for EIV infection among horses and Bactrian camels with influenza-like illness between national epizootics. In 2016-2017, active surveillance for EIV was periodically performed in four aimags (provinces). Nasal swabs were collected from 680 horses and 131 camels. Seven of the horse swabs were "positive" for qRT-PCR evidence of influenza A (Ct value ≤ 38). Two more were "suspect positive" (Ct value > 38 and ≤ 40). These nine specimens were collected from four aimags. None of the camel specimens had molecular evidence of infection. Despite serial blind passage in Madin-Darby Canine Kidney cells (MDCK) cells, none of the nine horse specimens yielded an influenza A virus. None of the 131 herder households surveyed had recently vaccinated their horses against EIV. It seems likely that sporadic EIV is enzootic in multiple Mongolian aimags. This finding, the infrequent use of EIV vaccination, periodic prevalence of highly pathogenic avian influenza, and the mixing of domestic and wild equid herds suggest that Mongolia may be a hot spot for novel EIV emergence.
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Publication Date: 2017-11-30 PubMed ID: 29189713PubMed Central: PMC5750585DOI: 10.3390/pathogens6040061Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study aimed to assess how widespread the equine influenza virus (EIV) is among horses in Mongolia, where horses are integral to local livelihoods. The research found that the EIV is likely present sporadically in horses across Mongolia, a fact that, coupled with infrequent vaccination and mixing of domestic and wild herds, suggests Mongolia may be a hotspot for the emergence of novel strains of EIV.

Study Background and Purpose

The researchers noted the significant role horses play in the livelihood of Mongolian herders, providing transportation, food, and cultural relevance. EIV has historically caused frequent disease outbreaks in Mongolian horses, with five significant collapses since 1970. The aim of this study was to approximate how prevalent EIV infection was among horses and Bactrian camels displaying influenza-like symptoms, particularly during periods between national epidemics.

Methodology

  • In 2016 and 2017, the researchers performed active surveillance for EIV periodically in four aimags, or provinces in Mongolia.
  • Nasal swabs were collected from a total of 680 horses and 131 camels.
  • The swabs were then tested for evidence of the influenza A virus with a procedure called quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR).
  • If a swab showed a Ct (Cycle threshold) value of less than or equal to 38, it was deemed “positive,” while swabs with Ct values greater than 38 but less than or equal to 40 were considered “suspect positive”.

Findings

  • Out of the 680 horse swabs taken, seven tested “positive” for EIV, while two more were dubbed “suspect positive”. These nine specimens came from all four aimags, indicating that the virus might be randomly scattered across the country.
  • No camel swabs showed any molecular signs of EIV infection.
  • Despite attempts to grow the virus in Madin-Darby Canine Kidney (MDCK) cells, none of the nine “positive” or “suspect positive” horse specimens yielded an influenza A virus.
  • A survey revealed that none of the 131 herder families had recently vaccinated their horses against EIV.

Conclusion and Implications

The researchers concluded that EIV is likely enzootic, or present in a sporadic and localized manner, in various parts of Mongolia. They cautioned that the infrequent use of EIV vaccine, coupled with the periodic presence of highly pathogenic avian influenza and the fact that domestic and wild equid herds often intermingle, might make Mongolia a hot spot for new strains of EIV to emerge.

Cite This Article

APA
Sack A, Daramragchaa U, Chuluunbaatar M, Gonchigoo B, Bazartseren B, Tsogbadrakh N, Gray GC. (2017). Low Prevalence of Enzootic Equine Influenza Virus among Horses in Mongolia. Pathogens, 6(4), 61. https://doi.org/10.3390/pathogens6040061

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 6
Issue: 4
PII: 61

Researcher Affiliations

Sack, Alexandra
  • Institute of Veterinary Medicine, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia. aesack16@gmail.com.
  • Division of Infectious Diseases, and Global Health Institute, Duke University, Durham, NC 27708, USA. aesack16@gmail.com.
Daramragchaa, Ulziimaa
  • Institute of Veterinary Medicine, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia. ulzima_d@yahoo.com.
Chuluunbaatar, Maitsetseg
  • Institute of Veterinary Medicine, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia. ch.maitsetseg@gmail.com.
Gonchigoo, Battsetseg
  • Institute of Veterinary Medicine, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia. gotsetseg@mail.mn.
Bazartseren, Boldbaatar
  • Institute of Veterinary Medicine, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia. boldoomglvet@yahoo.com.
Tsogbadrakh, Nyamdorj
  • National Center for Zoonotic Diseases, Ulaanbaatar 18131, Mongolia. tsogoo_0210@yahoo.com.
Gray, Gregory C
  • Division of Infectious Diseases, and Global Health Institute, Duke University, Durham, NC 27708, USA. gregory.gray@duke.edu.
  • Global Health Research Center, Duke-Kunshan University, Kunshan 215316, China. gregory.gray@duke.edu.
  • Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapur 169857, Singapore. gregory.gray@duke.edu.

Grant Funding

  • D43 TW009373 / FIC NIH HHS

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 26 references
  1. Eldevochir E. Livestock Statistics in Mongolia. 2016.
  2. United Nations Data Mongolia. [(accessed on 24 January 2017)]; Available online: http://data.un.org/CountryProfile.aspx?crname = Mongolia.
  3. Zhen L, Ochirbat B, Lv Y, Wei YJ, Liu XL, Chen JQ, Yao ZJ, Li F. Comparing patterns of ecosystem service consumption and perceptions of range management between ethnic herders in Inner Mongolia and Mongolia. Environ. Res. Lett. 2010;5:015001.
    doi: 10.1088/1748-9326/5/1/015001google scholar: lookup
  4. Bat-Oyun T, Erdenetsetseg B, Shinoda M, Ozaki T, Morinaga Y. Who is Making Airag (Fermented Mare’s Milk)? A Nationwide Survey of Traditional Food in Mongolia. Nomadic Peoples 2015;19:7–29.
    doi: 10.3197/np.2015.190103google scholar: lookup
  5. King SRB, Boyd L, Zimmermann W, Kendall BE. Equus ferus ssp. przewalskii. ICUN Red List 2015.
  6. Yin X, Lu G, Guo W, Qi T, Ma J, Zhu C, Zhao S, Pan J, Xiang W. Identification of equine influenza virus infection in Asian wild horses (Equus przewalskii).. Arch Virol 2014 May;159(5):1159-62.
    doi: 10.1007/s00705-013-1908-zpubmed: 24212887google scholar: lookup
  7. Gibbs EP, Anderson TC. Equine and canine influenza: a review of current events.. Anim Health Res Rev 2010 Jun;11(1):43-51.
    doi: 10.1017/S1466252310000046pubmed: 20426896google scholar: lookup
  8. Cullinane A, Newton JR. Equine influenza--a global perspective.. Vet Microbiol 2013 Nov 29;167(1-2):205-14.
    doi: 10.1016/j.vetmic.2013.03.029pubmed: 23680107google scholar: lookup
  9. Firestone SM, Cogger N, Ward MP, Toribio JA, Moloney BJ, Dhand NK. The influence of meteorology on the spread of influenza: survival analysis of an equine influenza (A/H3N8) outbreak.. PLoS One 2012;7(4):e35284.
    doi: 10.1371/journal.pone.0035284pmc: PMC3335077pubmed: 22536366google scholar: lookup
  10. Tellier R. Aerosol transmission of influenza A virus: a review of new studies.. J R Soc Interface 2009 Dec 6;6 Suppl 6(Suppl 6):S783-90.
    doi: 10.1098/rsif.2009.0302.focuspmc: PMC2843947pubmed: 19773292google scholar: lookup
  11. Powell DG, Watkins KL, Li PH, Shortridge KF. Outbreak of equine influenza among horses in Hong Kong during 1992.. Vet Rec 1995 May 27;136(21):531-6.
    doi: 10.1136/vr.136.21.531pubmed: 7660556google scholar: lookup
  12. Patterson-Kane JC, Carrick JB, Axon JE, Wilkie I, Begg AP. The pathology of bronchointerstitial pneumonia in young foals associated with the first outbreak of equine influenza in Australia.. Equine Vet J 2008 May;40(3):199-203.
    doi: 10.2746/042516408X292214pubmed: 18321807google scholar: lookup
  13. Webster RG. Are equine 1 influenza viruses still present in horses?. Equine Vet J 1993 Nov;25(6):537-8.
    doi: 10.1111/j.2042-3306.1993.tb03009.xpubmed: 8276003google scholar: lookup
  14. Karamendin K, Kydyrmanov A, Kasymbekov Y, Khan E, Daulbayeva K, Asanova S, Zhumatov K, Seidalina A, Sayatov M, Fereidouni SR. Continuing evolution of equine influenza virus in Central Asia, 2007-2012.. Arch Virol 2014 Sep;159(9):2321-7.
    doi: 10.1007/s00705-014-2078-3pubmed: 24748052google scholar: lookup
  15. Yondon M, Heil GL, Burks JP, Zayat B, Waltzek TB, Jamiyan BO, McKenzie PP, Krueger WS, Friary JA, Gray GC. Isolation and characterization of H3N8 equine influenza A virus associated with the 2011 epizootic in Mongolia.. Influenza Other Respir Viruses 2013 Sep;7(5):659-65.
    doi: 10.1111/irv.12069pmc: PMC3626732pubmed: 23289427google scholar: lookup
  16. Yondon M, Zayat B, Nelson MI, Heil GL, Anderson BD, Lin X, Halpin RA, McKenzie PP, White SK, Wentworth DE, Gray GC. Equine influenza A(H3N8) virus isolated from Bactrian camel, Mongolia.. Emerg Infect Dis 2014 Dec;20(12):2144-7.
    doi: 10.3201/eid2012.140435pmc: PMC4257804pubmed: 25418532google scholar: lookup
  17. Pagamjav O, Kobayashi K, Murakami H, Tabata Y, Miura Y, Boldbaatar B, Sentsui H. Serological survey of equine viral diseases in Mongolia.. Microbiol Immunol 2011 Apr;55(4):289-92.
    doi: 10.1111/j.1348-0421.2011.00312.xpmc: PMC7158856pubmed: 21447048google scholar: lookup
  18. Myers C, Wilson WD. Equine Influenza Virus. Clin. Tech. Equine Pract. 2006;5:187–196.
    doi: 10.1053/j.ctep.2006.03.013google scholar: lookup
  19. Leadon D, Waran N, Herholz C, Klay M. Veterinary management of horse transport.. Vet Ital 2008 Jan-Mar;44(1):149-63.
    pubmed: 20405422
  20. Landolt GA. Equine influenza virus.. Vet Clin North Am Equine Pract 2014 Dec;30(3):507-22.
    doi: 10.1016/j.cveq.2014.08.003pubmed: 25282321google scholar: lookup
  21. Faehrmann P, Riddell K, Read AJ. Longitudinal study describing the clinical signs observed in horses naturally infected with equine influenza.. Aust Vet J 2011 Jul;89 Suppl 1:22-3.
    doi: 10.1111/j.1751-0813.2011.00733.xpubmed: 21711276google scholar: lookup
  22. Dups JN, Morton JM, Anthony ND, Dwyer JF. Clinical signs of equine influenza in a closed population of horses at a 3-day event in southern Queensland, Australia.. Aust Vet J 2011 Jul;89 Suppl 1:17-8.
    doi: 10.1111/j.1751-0813.2011.00729.xpubmed: 21711274google scholar: lookup
  23. Xie T, Anderson BD, Daramragchaa U, Chuluunbaatar M, Gray GC. A Review of Evidence that Equine Influenza Viruses Are Zoonotic.. Pathogens 2016 Jul 12;5(3).
    doi: 10.3390/pathogens5030050pmc: PMC5039430pubmed: 27420100google scholar: lookup
  24. Morens DM, Taubenberger JK. Pandemic influenza: certain uncertainties.. Rev Med Virol 2011 Sep;21(5):262-84.
    doi: 10.1002/rmv.689pmc: PMC3246071pubmed: 21706672google scholar: lookup
  25. World Health Organization. WHO Information for Molecular Diagnosis of Influenza Virus—Update. World Health Organization 2014.
  26. World Health Organization. WHO Surveillance Case Definitions for ILI and SARI. World Health Organization 2014.

Citations

This article has been cited 8 times.
  1. Oladunni FS, Oseni SO, Martinez-Sobrido L, Chambers TM. Equine Influenza Virus and Vaccines. Viruses 2021 Aug 20;13(8).
    doi: 10.3390/v13081657pubmed: 34452521google scholar: lookup
  2. Miño S, Mojsiejczuk L, Guo W, Zhang H, Qi T, Du C, Zhang X, Wang J, Campos R, Wang X. Equine Influenza Virus in Asia: Phylogeographic Pattern and Molecular Features Reveal Circulation of an Autochthonous Lineage. J Virol 2019 Jul 1;93(13).
    doi: 10.1128/JVI.00116-19pubmed: 31019053google scholar: lookup
  3. Zhu H, Damdinjav B, Gonzalez G, Patrono LV, Ramirez-Mendoza H, Amat JAR, Crispell J, Parr YA, Hammond TA, Shiilegdamba E, Leung YHC, Peiris M, Marshall JF, Hughes J, Gilbert M, Murcia PR. Absence of adaptive evolution is the main barrier against influenza emergence in horses in Asia despite frequent virus interspecies transmission from wild birds. PLoS Pathog 2019 Feb;15(2):e1007531.
    doi: 10.1371/journal.ppat.1007531pubmed: 30731004google scholar: lookup
  4. Singh RK, Dhama K, Karthik K, Khandia R, Munjal A, Khurana SK, Chakraborty S, Malik YS, Virmani N, Singh R, Tripathi BN, Munir M, van der Kolk JH. A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies. Front Microbiol 2018;9:1941.
    doi: 10.3389/fmicb.2018.01941pubmed: 30237788google scholar: lookup
  5. Enkhbat M, Batzorig U, Dashdondog N, Trujillo-Vargas CM, Dambadarjaa D, Gray GC. The Highly Interrelated Morbidity Respiratory Viruses Cause Among Humans and Animals in Mongolia. Viruses 2025 Nov 28;17(12).
    doi: 10.3390/v17121557pubmed: 41472228google scholar: lookup
  6. Yang Y, Guo K, Xu L, Guo W, Dong M, Liu W, Li S, Zhang Z, Chu X, Wang Y, Zhang Z, Hu Z, Wang X. Development and application of a NP-cELISA for the detection of nucleoprotein antibodies of equine influenza virus. Microbiol Spectr 2025 Oct 7;13(10):e0093925.
    doi: 10.1128/spectrum.00939-25pubmed: 40853243google scholar: lookup
  7. Gonzalez-Obando J, Zuluaga-Cabrera A, Moreno I, Úsuga J, Ciuderis K, Forero JE, Diaz A, Rojas-Arbeláez C, Hernández-Ortiz JP, Ruiz-Saenz J. First Molecular Detection and Epidemiological Analysis of Equine Influenza Virus in Two Regions of Colombia, 2020-2023. Viruses 2024 May 24;16(6).
    doi: 10.3390/v16060839pubmed: 38932133google scholar: lookup
  8. Chambers TM. Equine Influenza. Cold Spring Harb Perspect Med 2022 Jan 4;12(1).
    doi: 10.1101/cshperspect.a038331pubmed: 32152243google scholar: lookup
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