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Journal of orthopaedic research : official publication of the Orthopaedic Research Society2009; 27(10); 1392-1398; doi: 10.1002/jor.20887

Mesenchymal stem cells and insulin-like growth factor-I gene-enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons.

Abstract: Tendinitis remains a catastrophic injury among athletes. Mesenchymal stem cells (MSCs) have recently been investigated for use in the treatment of tendinitis. Previous work has demonstrated the value of insulin-like growth factor-I (IGF-I) to stimulate cellular proliferation and tendon fiber deposition in the core lesion of tendinitis. This study examined the effects of MSCs, as well as IGF-I gene-enhanced MSCs (AdIGF-MSCs) on tendon healing in vivo. Collagenase-induced bilateral tendinitis lesions were created in equine flexor digitorum superficialis tendons (SDFT). Tendons were treated with 10 x 10(6) MSCs or 10 x 10(6) AdIGF-MSCs. Control limbs were injected with 1 mL of phosphate-buffered saline (PBS). Ultrasound examinations were performed at t = 0, 2, 4, 6, and 8 weeks. Horses were euthanized at 8 weeks and SDFTs were mechanically tested to failure and evaluated for biochemical composition and histologic characteristics. Expression of collagen types I and III, IGF-I, cartilage oligomeric matrix protein (COMP), matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-13 (MMP-13), and aggrecanase-1 (ADAMTS-4) were similar in MSC and control tendons. Both MSC and AdIGF-MSC injection resulted in significantly improved tendon histological scores. These findings indicate a benefit to the use of MSCs and AdIGF-MSCs for the treatment of tendinitis.
(c) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
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Publication Date: 2009-04-08 PubMed ID: 19350658DOI: 10.1002/jor.20887Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on exploring how Mesenchymal stem cells (MSCs) and insulin-like growth factor-I gene-enhanced Mesenchymal stem cells (AdIGF-MSCs) can contribute to the healing process of tendinitis, a common injury amongst athletes. The study indicates that MSCs and AdIGF-MSCs tend to improve tendon healing, thereby suggesting their potential use in tendinitis treatments.

Research Methodology

  • The study involved creating artificial tendinitis lesions in equine flexor digitorum superficialis tendons (SDFT) using collagenase, as equine tendons closely simulate the characteristics of human tendons.
  • The researchers then treated these tendons with either 10 x 10(6) MSCs or 10 x 10(6) AdIGF-MSCs. The control limbs were injected with 1 mL of phosphate-buffered saline (PBS) for comparison.
  • Ultrasound examinations were conducted at different time intervals (t = 0, 2, 4, 6, 8 weeks) to observe the progress of tendon healing.
  • Eight weeks post-treatment, the SDFTs were thoroughly examined for their mechanical strength, biochemical composition, and histologic characteristics to discern potential improvements.

Results and Findings

  • Researchers found no variation in the expression of collagen types I and III, IGF-I, cartilage oligomeric matrix protein (COMP), matrix metalloproteinase-13 (MMP-13), aggrecanase-1 (ADAMTS-4), and matrix metalloproteinase-3 (MMP-3) between the MSC treated and control tendons.
  • However, both MSC and AdIGF-MSC injections significantly improved the histological scores of the tendon, suggesting better healing outcomes.
  • These results provide evidence for the therapeutic potential of MSCs and AdIGF-MSCs in tendinitis treatment, whereby they may catalyze the healing process and improve tendon structure and function post-injury.

Conclusion

Overall, the study supports the application of Mesenchymal stem cells and insulin-like growth factor-I gene-enhanced Mesenchymal stem cells for treating tendinitis. However, further studies may be required to understand the long-term implications and side effects of these advancements amidst different variable factors.

Cite This Article

APA
Schnabel LV, Lynch ME, van der Meulen MC, Yeager AE, Kornatowski MA, Nixon AJ. (2009). Mesenchymal stem cells and insulin-like growth factor-I gene-enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons. J Orthop Res, 27(10), 1392-1398. https://doi.org/10.1002/jor.20887

Publication

Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
NlmUniqueID: 8404726
Country: United States
Language: English
Volume: 27
Issue: 10
Pages: 1392-1398

Researcher Affiliations

Schnabel, Lauren V
  • Comparative Orthopaedics Laboratory, Department of Clinical Sciences, C3-187 Veterinary Medical Center, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
Lynch, Maureen E
    van der Meulen, Marjolein C H
      Yeager, Amy E
        Kornatowski, Matthew A
          Nixon, Alan J

            MeSH Terms

            • ADAM Proteins / metabolism
            • ADAMTS4 Protein
            • Animals
            • Biomechanical Phenomena
            • Cell- and Tissue-Based Therapy / methods
            • Cell- and Tissue-Based Therapy / veterinary
            • Collagen Type I / metabolism
            • Collagen Type III / metabolism
            • Extracellular Matrix Proteins / metabolism
            • Female
            • Genetic Therapy / methods
            • Genetic Therapy / veterinary
            • Glycoproteins / metabolism
            • Horse Diseases / metabolism
            • Horse Diseases / pathology
            • Horse Diseases / therapy
            • Horses
            • Insulin-Like Growth Factor I / genetics
            • Insulin-Like Growth Factor I / metabolism
            • Male
            • Matrilin Proteins
            • Matrix Metalloproteinase 13 / metabolism
            • Matrix Metalloproteinase 3 / metabolism
            • Mesenchymal Stem Cell Transplantation / methods
            • Mesenchymal Stem Cell Transplantation / veterinary
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / metabolism
            • Procollagen N-Endopeptidase / metabolism
            • Tendinopathy / metabolism
            • Tendinopathy / therapy
            • Tendinopathy / veterinary
            • Tendons / diagnostic imaging
            • Tendons / metabolism
            • Tendons / pathology
            • Ultrasonography

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