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Home / Equine Research Bank / Stem Cell Rev Rep / Mesenchymal Stem Cells Isolated from Equine Hair Follicles U...
Stem cell reviews and reports2023; 19(8); 2943-2956; doi: 10.1007/s12015-023-10619-w

Mesenchymal Stem Cells Isolated from Equine Hair Follicles Using a Method of Air-Liquid Interface.

Abstract: Equine mesenchymal stem cells (MSC) of various origins have been identified in horses, including MSCs from the bone marrow and adipose tissue. However, these stem cell sources are highly invasive in sampling, which thereby limits their clinical application in equine veterinary medicine. This study presents a novel method using an air-liquid interface to isolate stem cells from the hair follicle outer root sheath of the equine forehead skin. These stem cells cultured herewith showed high proliferation and asumed MSC phenotype by expressing MSC positive biomarkers (CD29, CD44 CD90) while not expressing negative markers (CD14, CD34 and CD45). They were capable of differentiating towards chondrogenic, osteogenic and adipogenic lineages, which was comparable with MSCs from adipose tissue. Due to their proliferative phenotype in vitro, MSC-like profile and differentiation capacities, we named them equine mesenchymal stem cells from the hair follicle outer root sheath (eMSCORS). eMSCORS present a promising alternative stem cell source for the equine veterinary medicine.
© 2023. The Author(s).
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Publication Date: 2023-09-21 PubMed ID: 37733199PubMed Central: PMC10661790DOI: 10.1007/s12015-023-10619-wGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a novel method of isolating mesenchymal stem cells from the hair follicles of horses for use in veterinary medicine, which offers a less invasive alternative to obtaining stem cells from bone marrow and adipose tissue.

Methodology

  • The researchers isolated stem cells from the outer root sheath of the hair follicle of the equine forehead skin. The novel method they used is known as the air-liquid interface.
  • The stem cells obtained were cultured for potential medical applications.

Findings

  • The stem cells (named as equine mesenchymal stem cells from the hair follicle outer root sheath, or eMSCORS) showed high proliferation and expressed mesenchymal stem cell (MSC) positive biomarkers including CD29, CD44, and CD90.
  • eMSCORS did not express negative markers such as CD14, CD34, and CD45, which further validates their MSC phenotype.
  • In terms of differentiation capacity, the eMSCORS were able to differentiate towards chondrogenic, osteogenic, and adipogenic lineages; this was comparable with mesenchymal stem cells obtained from adipose tissue.

Implications

  • The results of the study suggest that equine hair follicles could serve as a promising source of non-invasive and efficient stem cell derivation which could be beneficial in equine veterinary medicine.
  • The use of non-invasive stem cell sources such as hair follicles reduces the potential risk and discomfort to the horse compared to invasive procedures like sampling bone marrow or adipose tissue.
  • Finally, due to the high proliferation and differentiation capacities of the eMSCORS, they could be used in a range of veterinary medicinal applications such as tissue repair and regeneration.

Cite This Article

APA
(2023). Mesenchymal Stem Cells Isolated from Equine Hair Follicles Using a Method of Air-Liquid Interface. Stem Cell Rev Rep, 19(8), 2943-2956. https://doi.org/10.1007/s12015-023-10619-w

Publication

Stem cell reviews and reports
ISSN: 2629-3277
NlmUniqueID: 101752767
Country: United States
Language: English
Volume: 19
Issue: 8
Pages: 2943-2956

Researcher Affiliations

MeSH Terms

  • Animals
  • Horses
  • Hair Follicle
  • Mesenchymal Stem Cells
  • Stem Cells
  • Adipogenesis
  • Adipose Tissue

Grant Funding

  • SIKT Teilprojekt 34707001 / SMWK 34707004 / Su00e4chsisches Staatsministerium fu00fcr Wissenschaft und Kunst
  • grant No.2021CFB289 / Natural Science Foundation of Hubei Province

Conflict of Interest Statement

The authors declare no conflict of interest.

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