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Journal of veterinary internal medicine2018; 32(3); 1215-1233; doi: 10.1111/jvim.15095

Metabolic perturbations in Welsh Ponies with insulin dysregulation, obesity, and laminitis.

Abstract: Metabolomics, the study of small-molecule metabolites, has increased understanding of human metabolic diseases, but has not been used to study equine metabolic syndrome (EMS). Objective: (1) To examine the serum metabolome of Welsh Ponies with and without insulin dysregulation before and during an oral sugar test (OST). (2) To identify differences in metabolites in ponies with insulin dysregulation, obesity, or history of laminitis. Methods: Twenty Welsh Ponies (mean ± SD; 13.8 ± 9.0 years) classified as non-insulin dysregulated [CON] (n = 10, insulin  60 mU/L) at 75 minutes after administration of Karo syrup, obese (n = 6) or nonobese (n = 14), and history of laminitis (n = 9) or no history of laminitis (n = 11). Methods: Case-control study. Metabolomic analysis was performed on serum obtained at 0 minutes (baseline) and 75 minutes during the OST. Data were analyzed with multivariable mixed linear models with significance set at P ≤ .05. Results: Metabolomic analysis of 646 metabolites (506 known) detected significant metabolite differences. At baseline, 55 metabolites (insulin response), 91 metabolites (obesity status), and 136 metabolites (laminitis history) were different. At 75 minutes, 51 metabolites (insulin response), 102 metabolites (obesity status), and 124 metabolites (laminitis history) were different. Conclusions: Use of metabolomics could have diagnostic utility for early detection of EMS and provide new knowledge regarding the pathophysiology of metabolic perturbations associated with this condition that might lead to improved clinical management.
Copyright © 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2018-03-23 PubMed ID: 29572947PubMed Central: PMC5980341DOI: 10.1111/jvim.15095Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research studies the presence and variations of small-molecule metabolites in Welsh Ponies to understand insulin dysregulation, obesity, and laminitis. It aims to identify any distinct metabolic markers that can help diagnose equine metabolic syndrome (EMS) earlier and improve its clinical management.

Objective

The key objective of the study was twofold:

  • The researchers wanted to explore the serum metabolome of Welsh Ponies with and without insulin dysregulation, both before and while under an oral sugar test (OST).
  • The aim was also to identify differences in metabolites in Welsh Ponies with insulin dysregulation, obesity, or has had a history of laminitis.

Methods

The study was performed as a case-control experiment with the following key aspects:

  • The research sample included twenty Welsh Ponies with a mean of 13.8±9.0 years. The ponies were categorised based on whether they were non-insulin dysregulated or insulin-dysregulated, obese or non-obese, and if they had a history of laminitis or not.
  • The serum was subjected to metabolomic analysis at 0 minutes (baseline) and 75 minutes during the OST.
  • Metabolic data was then processed using multivariable mixed linear models with significance set at P ≤ .05.

Results

The results from the metabolomic analysis of 646 metabolites (of which, 506 were known) showed significant differences:

  • At the baseline, distinct differences in 55 metabolites (relating to insulin response), 91 metabolites (relating to obesity status), and 136 metabolites (relating to laminitis history) were observed.
  • At 75 minutes into the OST, the study noticed variations in 51 metabolites (in relation to insulin response), 102 metabolites (with respect to obesity status), and 124 metabolites (in regards to laminitis history).

Conclusions

This study concludes that the use of metabolomics could:

  • Potentially be a useful diagnostic tool for early detection of equine metabolic syndrome (EMS).
  • Provide new knowledge about the metabolic perturbations linked to EMS, thereby guiding improved clinical management of this condition.

Cite This Article

APA
Jacob SI, Murray KJ, Rendahl AK, Geor RJ, Schultz NE, McCue ME. (2018). Metabolic perturbations in Welsh Ponies with insulin dysregulation, obesity, and laminitis. J Vet Intern Med, 32(3), 1215-1233. https://doi.org/10.1111/jvim.15095

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 32
Issue: 3
Pages: 1215-1233

Researcher Affiliations

Jacob, Sarah I
  • Michigan State University College of Veterinary Medicine, Large Animal Clinical Sciences, East Lansing, Michigan.
Murray, Kevin J
  • University of Minnesota College of Veterinary Medicine, Veterinary Population Medicine, St. Paul, Minnesota.
Rendahl, Aaron K
  • University of Minnesota College of Veterinary Medicine, Veterinary Population Medicine, St. Paul, Minnesota.
Geor, Raymond J
  • Massey University College of Sciences, Palmerston North, New Zealand.
Schultz, Nichol E
  • University of Minnesota College of Veterinary Medicine, Veterinary Population Medicine, St. Paul, Minnesota.
McCue, Molly E
  • University of Minnesota College of Veterinary Medicine, Veterinary Population Medicine, St. Paul, Minnesota.

MeSH Terms

  • Animals
  • Blood Glucose / analysis
  • Female
  • Foot Diseases / metabolism
  • Foot Diseases / veterinary
  • Glucose Tolerance Test / veterinary
  • Hoof and Claw
  • Horse Diseases / metabolism
  • Horses
  • Insulin / blood
  • Insulin / metabolism
  • Male
  • Metabolic Syndrome / metabolism
  • Metabolic Syndrome / veterinary
  • Metabolomics
  • Obesity / metabolism
  • Obesity / veterinary

Grant Funding

  • T32 AR007612 / NIAMS NIH HHS

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