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Animals : an open access journal from MDPI2021; 11(7); 1913; doi: 10.3390/ani11071913

Preliminary Study: Proteomic Profiling Uncovers Potential Proteins for Biomonitoring Equine Melanocytic Neoplasm.

Abstract: Equine melanocytic neoplasm (EMN) is a cutaneous neoplasm and is mostly observed in aged grey horses. This preliminary study aimed to identify potential proteins to differentiate normal, mild and severe EMN from serum proteomic profiling. Serum samples were collected from 25 grey horses assigned to three groups: normal (free of EMN; = 10), mild ( = 6) and severe EMN ( = 9). To explore the differences in proteins between groups, proteomic profiling and analysis were employed. Accordingly, 8241 annotated proteins out of 8725 total proteins were compared between normal and EMN groups and inspected based on differentially expressed proteins (DEPs). Through DEP analysis, 95 significant DEPs differed between normal and EMN groups. Among these DEPs, 41 significant proteins were categorised according to protein functions. Based on 41 significant proteins, 10 were involved in metabolism and 31 in non-metabolism. Interestingly, phospholipid phosphatase6 (PLPP6) and ATPase subunit alpha (Na+/K+-ATPase) were considered as potential proteins uniquely expressed in mild EMN and related to lipid and energy metabolism, respectively. Non-metabolism-related proteins (BRCA1, phosphorylase B kinase regulatory subunit: PHKA1, tyrosine-protein kinase receptor: ALK and rho-associated protein kinase: ROCK1) correlated to melanoma development differed among all groups. The results of our study provide a foundation for early EMN biomonitoring and prevention.
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Publication Date: 2021-06-27 PubMed ID: 34199079PubMed Central: PMC8300200DOI: 10.3390/ani11071913Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a preliminary study where scientists utilized proteomic profiling to identify unique proteins in the serum of grey horses that may indicate the presence or severity of Equine Melanocytic Neoplasm (EMN), a type of skin cancer.

Methodology

  • Scientists collected serum samples from 25 grey horses categorized based on their EMN status: normal ( = 10), mild EMN ( = 6), and severe EMN ( = 9).
  • They used proteomic profiling (analyzing the specific proteins present in the serum), and a comparative analysis, focusing on differentially expressed proteins (DEPs) to identify variations in the proteins present in the different groups.
  • Of the total of 8725 proteins analysed, 8241 were annotated and compared between the normal and EMN groups.

Findings

  • 95 significant DEPs were identified, differing between normal and EMN groups.
  • Out of these differentially expressed proteins, 41 were identified as most crucial and were classified according to their functions.
  • 10 of these proteins were involved in metabolism, and 31 were related to non-metabolic functions.
  • Two proteins, phospholipid phosphatase6 (PLPP6) and ATPase subunit alpha (Na+/K+-ATPase), were uniquely expressed in the serum of horses with mild EMN. These proteins are believed to be related to lipid and energy metabolism, respectively.
  • Non-metabolic proteins (BRCA1, phosphorylase B kinase regulatory subunit: PHKA1, tyrosine-protein kinase receptor: ALK and rho-associated protein kinase: ROCK1), were found to be linked with melanoma development, differed amongst all groups.

Conclusion

  • The results of this preliminary study provide potential markers for early biomonitoring and prevention of EMN.
  • Further studies are required to validate these findings and develop a practical diagnostic tool or preventive treatment for EMN using these biomarkers.

Cite This Article

APA
Tesena P, Kingkaw A, Vongsangnak W, Pitikarn S, Phaonakrop N, Roytrakul S, Kovitvadhi A. (2021). Preliminary Study: Proteomic Profiling Uncovers Potential Proteins for Biomonitoring Equine Melanocytic Neoplasm. Animals (Basel), 11(7), 1913. https://doi.org/10.3390/ani11071913

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 7
PII: 1913

Researcher Affiliations

Tesena, Parichart
  • Graduate Student in Animal Health and Biomedical Science Program, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom 73170, Thailand.
Kingkaw, Amornthep
  • Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Department of Zoology, Faculty of Sciences, Kasetsart University, Bangkok 10900, Thailand.
Vongsangnak, Wanwipa
  • Department of Zoology, Faculty of Sciences, Kasetsart University, Bangkok 10900, Thailand.
  • Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok 10900, Thailand.
Pitikarn, Surakiet
  • Genetic Engineering and Bioinformatics Program, Graduate School, Kasetsart University, Bangkok 10900, Thailand.
Phaonakrop, Narumon
  • Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand.
Roytrakul, Sittiruk
  • Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand.
Kovitvadhi, Attawit
  • Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Grant Funding

  • P1950420 / Functional ingredient and Food innovation program, National Science and Technology Develop-ment Agency
  • 63 / Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

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