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Home / Equine Research Bank / Front Vet Sci / Prospects and Challenges of Induced Pluripotent Stem Cells i...
Frontiers in veterinary science2015; 2; 59; doi: 10.3389/fvets.2015.00059

Prospects and Challenges of Induced Pluripotent Stem Cells in Equine Health.

Abstract: Pluripotent stem cells (PSCs) hold, through the capacity to differentiate into virtually all body cell types, unprecedented promise for human and animal medicine. PSCs are naturally found in the early embryo, and in rodents and humans they can be robustly harvested and grown in culture in the form of embryonic stem cells (ESCs); however, the availability of ESCs from horses is limited. ES-like cells named induced pluripotent stem cells (iPSCs) can be derived in vitro by transcription factor-mediated reprogramming of adult cells. As such, iPSCs can be generated in a patient-specific manner providing unmatched potential for tissue transplantation and in vitro disease modeling. In humans, clinical trials using iPSC-derived cells are already taking place and the use of in vitro iPSC models has identified novel mechanisms of disease and therapeutic targets. Although to a more limited extent, iPSCs have also been generated from horses, a species in which, after humans, these cells are likely to hold the greatest potential in regenerative medicine. Before a clinical use can be envisioned, however, significant challenges will need to be addressed in relation to the robust derivation, long-term culture, differentiation, and clinical safety of equine iPSCs. Toward this objective, recent studies have reported significant improvement in culture conditions and the successful derivation for the first time of functional cell types from equine iPSCs. Given the wide range of exciting applications they could have, it is hoped future research will make the biomedical promise of iPSCs a reality not only for humans but also horses.
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Publication Date: 2015-11-19 PubMed ID: 26664986PubMed Central: PMC4672244DOI: 10.3389/fvets.2015.00059Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the potential and challenges of utilizing induced pluripotent stem cells (iPSCs) in the field of equine health. It presents an examination of how these cells, which have the ability to grow into any type of body cell, could significantly advance medical treatments for horses.

Induced Pluripotent Stem Cells (iPSCs)

  • The research focuses on pluripotent stem cells (PSCs) specifically induced pluripotent stem cells (iPSCs). These cells have the ability to differentiate into virtually all body cell types.
  • PSCs in general are found in the early embryo, and in humans and rodents, can be harvested and grown robustly in the lab in the form of embryonic stem cells (ESCs).
  • Unlike ESCs, iPSCs can be derived in vitro through reprogramming with transcription factors of adult cells, enabling generation in a patient-specific manner. This opens up prospects for tissue transplantation and in vitro disease modeling.

Application in Horses

  • The paper highlights the potential use of iPSCs in horses. After humans, horses are stated as a species where iPSCs have the greatest potential in regenerative medicine.
  • iPSCs have been generated in horses to some extent, but their biomedical promise requires further investigation through future research.

Challenges and Progress

  • Before clinical use of equine iPSCs can be considered, several challenges need addressing, including the robust derivation, long-term culture, differentiation, and clinical safety of these cells.
  • Recent studies have reported improvements in culture conditions and the successful derivation for the first time of functional cell types from equine iPSCs, showing progress in this field.

Conclusion

  • The article advocates for the continued research of iPSCs, given the wide range of applications they could have in equine health, while highlighting the significant steps that are required to fully unlock their potential.

Cite This Article

APA
Donadeu FX, Esteves CL. (2015). Prospects and Challenges of Induced Pluripotent Stem Cells in Equine Health. Front Vet Sci, 2, 59. https://doi.org/10.3389/fvets.2015.00059

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 2
Pages: 59
PII: 59

Researcher Affiliations

Donadeu, F Xavier
  • The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh , Midlothian , UK.
Esteves, Cristina L
  • The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh , Midlothian , UK.

References

This article includes 39 references
  1. Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos.. Nature 1981 Jul 9;292(5819):154-6.
    doi: 10.1038/292154a0pubmed: 7242681google scholar: lookup
  2. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. Embryonic stem cell lines derived from human blastocysts.. Science 1998 Nov 6;282(5391):1145-7.
    doi: 10.1126/science.282.5391.1145pubmed: 9804556google scholar: lookup
  3. Li X, Zhou SG, Imreh MP, Ahrlund-Richter L, Allen WR. Horse embryonic stem cell lines from the proliferation of inner cell mass cells.. Stem Cells Dev 2006 Aug;15(4):523-31.
    doi: 10.1089/scd.2006.15.523pubmed: 16978056google scholar: lookup
  4. Saito S, Sawai K, Minamihashi A, Ugai H, Murata T, Yokoyama KK. Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells.. Methods Mol Biol 2006;329:59-79.
    doi: 10.1385/1-59745-037-5:59pubmed: 16845984google scholar: lookup
  5. Guest DJ, Smith MR, Allen WR. Equine embryonic stem-like cells and mesenchymal stromal cells have different survival rates and migration patterns following their injection into damaged superficial digital flexor tendon.. Equine Vet J 2010 Oct;42(7):636-42.
    doi: 10.1111/j.2042-3306.2010.00112.xpubmed: 20840579google scholar: lookup
  6. Barsby T, Bavin EP, Guest DJ. Three-dimensional culture and transforming growth factor beta3 synergistically promote tenogenic differentiation of equine embryo-derived stem cells.. Tissue Eng Part A 2014 Oct;20(19-20):2604-13.
    doi: 10.1089/ten.TEA.2013.0457pmc: PMC4195467pubmed: 24628376google scholar: lookup
  7. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.. Cell 2006 Aug 25;126(4):663-76.
    doi: 10.1016/j.cell.2006.07.024pubmed: 16904174google scholar: lookup
  8. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.. Cell 2007 Nov 30;131(5):861-72.
    doi: 10.1016/j.cell.2007.11.019pubmed: 18035408google scholar: lookup
  9. Ezashi T, Telugu BP, Alexenko AP, Sachdev S, Sinha S, Roberts RM. Derivation of induced pluripotent stem cells from pig somatic cells.. Proc Natl Acad Sci U S A 2009 Jul 7;106(27):10993-8.
    doi: 10.1073/pnas.0905284106pmc: PMC2698893pubmed: 19541600google scholar: lookup
  10. Shimada H, Nakada A, Hashimoto Y, Shigeno K, Shionoya Y, Nakamura T. Generation of canine induced pluripotent stem cells by retroviral transduction and chemical inhibitors.. Mol Reprod Dev 2010 Jan;77(1):2.
    doi: 10.1002/mrd.21117pubmed: 19890968google scholar: lookup
  11. Li Y, Cang M, Lee AS, Zhang K, Liu D. Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.. PLoS One 2011 Jan 6;6(1):e15947.
    doi: 10.1371/journal.pone.0015947pmc: PMC3017083pubmed: 21253598google scholar: lookup
  12. Sumer H, Liu J, Malaver-Ortega LF, Lim ML, Khodadadi K, Verma PJ. NANOG is a key factor for induction of pluripotency in bovine adult fibroblasts.. J Anim Sci 2011 Sep;89(9):2708-16.
    doi: 10.2527/jas.2010-3666pubmed: 21478453google scholar: lookup
  13. Nagy K, Sung HK, Zhang P, Laflamme S, Vincent P, Agha-Mohammadi S, Woltjen K, Monetti C, Michael IP, Smith LC, Nagy A. Induced pluripotent stem cell lines derived from equine fibroblasts.. Stem Cell Rev Rep 2011 Sep;7(3):693-702.
    doi: 10.1007/s12015-011-9239-5pmc: PMC3137777pubmed: 21347602google scholar: lookup
  14. Khodadadi K, Sumer H, Pashaiasl M, Lim S, Williamson M, Verma PJ. Induction of pluripotency in adult equine fibroblasts without c-MYC.. Stem Cells Int 2012;2012:429160.
    doi: 10.1155/2012/429160pmc: PMC3328202pubmed: 22550508google scholar: lookup
  15. Breton A, Sharma R, Diaz AC, Parham AG, Graham A, Neil C, Whitelaw CB, Milne E, Donadeu FX. Derivation and characterization of induced pluripotent stem cells from equine fibroblasts.. Stem Cells Dev 2013 Feb 15;22(4):611-21.
    doi: 10.1089/scd.2012.0052pmc: PMC3564467pubmed: 22897112google scholar: lookup
  16. Sharma R, Livesey MR, Wyllie DJ, Proudfoot C, Whitelaw CB, Hay DC, Donadeu FX. Generation of functional neurons from feeder-free, keratinocyte-derived equine induced pluripotent stem cells.. Stem Cells Dev 2014 Jul 1;23(13):1524-34.
    doi: 10.1089/scd.2013.0565pubmed: 24548115google scholar: lookup
  17. Whitworth DJ, Ovchinnikov DA, Sun J, Fortuna PR, Wolvetang EJ. Generation and characterization of leukemia inhibitory factor-dependent equine induced pluripotent stem cells from adult dermal fibroblasts.. Stem Cells Dev 2014 Jul 1;23(13):1515-23.
    doi: 10.1089/scd.2013.0461pmc: PMC4066230pubmed: 24555755google scholar: lookup
  18. Aasen T, Izpisúa Belmonte JC. Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells.. Nat Protoc 2010 Feb;5(2):371-82.
    doi: 10.1038/nprot.2009.241pubmed: 20134422google scholar: lookup
  19. Aguiar C, Therrien J, Lemire P, Segura M, Smith LC, Theoret CL. Differentiation of equine induced pluripotent stem cells into a keratinocyte lineage.. Equine Vet J 2016 May;48(3):338-45.
    doi: 10.1111/evj.12438pubmed: 25781637google scholar: lookup
  20. Chan EM, Ratanasirintrawoot S, Park IH, Manos PD, Loh YH, Huo H, Miller JD, Hartung O, Rho J, Ince TA, Daley GQ, Schlaeger TM. Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells.. Nat Biotechnol 2009 Nov;27(11):1033-7.
    doi: 10.1038/nbt.1580pubmed: 19826408google scholar: lookup
  21. Okano H, Nakamura M, Yoshida K, Okada Y, Tsuji O, Nori S, Ikeda E, Yamanaka S, Miura K. Steps toward safe cell therapy using induced pluripotent stem cells.. Circ Res 2013 Feb 1;112(3):523-33.
    doi: 10.1161/CIRCRESAHA.111.256149pubmed: 23371901google scholar: lookup
  22. Wernig M, Zhao JP, Pruszak J, Hedlund E, Fu D, Soldner F, Broccoli V, Constantine-Paton M, Isacson O, Jaenisch R. Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease.. Proc Natl Acad Sci U S A 2008 Apr 15;105(15):5856-61.
    doi: 10.1073/pnas.0801677105pmc: PMC2311361pubmed: 18391196google scholar: lookup
  23. Tucker BA, Park IH, Qi SD, Klassen HJ, Jiang C, Yao J, Redenti S, Daley GQ, Young MJ. Transplantation of adult mouse iPS cell-derived photoreceptor precursors restores retinal structure and function in degenerative mice.. PLoS One 2011 Apr 29;6(4):e18992.
    doi: 10.1371/journal.pone.0018992pmc: PMC3084746pubmed: 21559507google scholar: lookup
  24. Hanna J, Wernig M, Markoulaki S, Sun CW, Meissner A, Cassady JP, Beard C, Brambrink T, Wu LC, Townes TM, Jaenisch R. Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin.. Science 2007 Dec 21;318(5858):1920-3.
    doi: 10.1126/science.1152092pubmed: 18063756google scholar: lookup
  25. Tedesco FS, Gerli MF, Perani L, Benedetti S, Ungaro F, Cassano M, Antonini S, Tagliafico E, Artusi V, Longa E, Tonlorenzi R, Ragazzi M, Calderazzi G, Hoshiya H, Cappellari O, Mora M, Schoser B, Schneiderat P, Oshimura M, Bottinelli R, Sampaolesi M, Torrente Y, Broccoli V, Cossu G. Transplantation of genetically corrected human iPSC-derived progenitors in mice with limb-girdle muscular dystrophy.. Sci Transl Med 2012 Jun 27;4(140):140ra89.
    doi: 10.1126/scitranslmed.3003541pubmed: 22745439google scholar: lookup
  26. Aguiar C, Theoret C, Smith O, Segura M, Lemire P, Smith LC. Immune potential of allogeneic equine induced pluripotent stem cells.. Equine Vet J 2015 Nov;47(6):708-14.
    pubmed: 25196173doi: 10.1111/evj.12345google scholar: lookup
  27. Lian Q, Zhang Y, Zhang J, Zhang HK, Wu X, Zhang Y, Lam FF, Kang S, Xia JC, Lai WH, Au KW, Chow YY, Siu CW, Lee CN, Tse HF. Functional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice.. Circulation 2010 Mar 9;121(9):1113-23.
    doi: 10.1161/CIRCULATIONAHA.109.898312pubmed: 20176987google scholar: lookup
  28. Waese EY, Stanford WL. One-step generation of murine embryonic stem cell-derived mesoderm progenitors and chondrocytes in a serum-free monolayer differentiation system.. Stem Cell Res 2011 Jan;6(1):34-49.
    doi: 10.1016/j.scr.2010.08.007pubmed: 20920900google scholar: lookup
  29. Dar A, Domev H, Ben-Yosef O, Tzukerman M, Zeevi-Levin N, Novak A, Germanguz I, Amit M, Itskovitz-Eldor J. Multipotent vasculogenic pericytes from human pluripotent stem cells promote recovery of murine ischemic limb.. Circulation 2012 Jan 3;125(1):87-99.
    doi: 10.1161/CIRCULATIONAHA.111.048264pubmed: 22095829google scholar: lookup
  30. Robinton DA, Daley GQ. The promise of induced pluripotent stem cells in research and therapy.. Nature 2012 Jan 18;481(7381):295-305.
    doi: 10.1038/nature10761pmc: PMC3652331pubmed: 22258608google scholar: lookup
  31. Bellin M, Marchetto MC, Gage FH, Mummery CL. Induced pluripotent stem cells: the new patient?. Nat Rev Mol Cell Biol 2012 Nov;13(11):713-26.
    doi: 10.1038/nrm3448pubmed: 23034453google scholar: lookup
  32. Mayshar Y, Ben-David U, Lavon N, Biancotti JC, Yakir B, Clark AT, Plath K, Lowry WE, Benvenisty N. Identification and classification of chromosomal aberrations in human induced pluripotent stem cells.. Cell Stem Cell 2010 Oct 8;7(4):521-31.
    doi: 10.1016/j.stem.2010.07.017pubmed: 20887957google scholar: lookup
  33. O'Malley J, Woltjen K, Kaji K. New strategies to generate induced pluripotent stem cells.. Curr Opin Biotechnol 2009 Oct;20(5):516-21.
    doi: 10.1016/j.copbio.2009.09.005pubmed: 19837580google scholar: lookup
  34. Hou P, Li Y, Zhang X, Liu C, Guan J, Li H, Zhao T, Ye J, Yang W, Liu K, Ge J, Xu J, Zhang Q, Zhao Y, Deng H. Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds.. Science 2013 Aug 9;341(6146):651-4.
    doi: 10.1126/science.1239278pubmed: 23868920google scholar: lookup
  35. Telugu BP, Ezashi T, Roberts RM. The promise of stem cell research in pigs and other ungulate species.. Stem Cell Rev Rep 2010 Mar;6(1):31-41.
    doi: 10.1007/s12015-009-9101-1pubmed: 19949895google scholar: lookup
  36. Zhou L, Wang W, Liu Y, Fernandez de Castro J, Ezashi T, Telugu BP, Roberts RM, Kaplan HJ, Dean DC. Differentiation of induced pluripotent stem cells of swine into rod photoreceptors and their integration into the retina.. Stem Cells 2011 Jun;29(6):972-80.
    doi: 10.1002/stem.637pmc: PMC4263955pubmed: 21491544google scholar: lookup
  37. Morizane A, Doi D, Kikuchi T, Okita K, Hotta A, Kawasaki T, Hayashi T, Onoe H, Shiina T, Yamanaka S, Takahashi J. Direct comparison of autologous and allogeneic transplantation of iPSC-derived neural cells in the brain of a non-human primate.. Stem Cell Reports 2013;1(4):283-92.
    doi: 10.1016/j.stemcr.2013.08.007pmc: PMC3849265pubmed: 24319664google scholar: lookup
  38. Kaneko S, Yamanaka S. To be immunogenic, or not to be: that's the iPSC question.. Cell Stem Cell 2013 Apr 4;12(4):385-6.
    doi: 10.1016/j.stem.2013.03.008pubmed: 23561437google scholar: lookup
  39. Wilmut I, Leslie S, Martin NG, Peschanski M, Rao M, Trounson A, Turner D, Turner ML, Yamanaka S, Taylor CJ. Development of a global network of induced pluripotent stem cell haplobanks.. Regen Med 2015;10(3):235-8.
    doi: 10.2217/rme.15.1pubmed: 25933231google scholar: lookup

Citations

This article has been cited 2 times.
  1. Pessôa LVF, Bressan FF, Freude KK. Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.. World J Stem Cells 2019 Aug 26;11(8):491-505.
    doi: 10.4252/wjsc.v11.i8.491pubmed: 31523369google scholar: lookup
  2. Esteves CL, Sheldrake TA, Mesquita SP, Pesántez JJ, Menghini T, Dawson L, Péault B, Donadeu FX. Isolation and characterization of equine native MSC populations.. Stem Cell Res Ther 2017 Apr 18;8(1):80.
    doi: 10.1186/s13287-017-0525-2pubmed: 28420427google scholar: lookup
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