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Home / Equine Research Bank / Vet Med Sci / Sternal bone marrow derived equine multipotent mesenchymal s...
Veterinary medicine and science2016; 2(3); 200-210; doi: 10.1002/vms3.36

Sternal bone marrow derived equine multipotent mesenchymal stromal cells (MSCs): investigations considering the sampling site and the use of different culture media.

Abstract: Aspiration of equine sternal bone marrow is required for the cultivation of bone marrow-derived multipotent mesenchymal stromal cells (BM-MSCs) for regenerative therapies. For bone marrow aspiration as well as for MSC cultivation, there is a need to optimize techniques and protocols to enhance MSC harvest at minimized culture times. In a comparative study bone marrow aspirates from sternebra 4 and 5 were collected at two different positions within the sternebrae, either from 10 mm or from 30 mm dorsal from the ventral margin of the sternebrae. Accuracy of the puncture depth was confirmed by ultrasonography and computed tomography. Isolated MSCs were cultivated using media supplemented with three alternative sera, i.e. fetal calf serum, standardized horse serum and autologous serum. Due to morphological characteristics (spherical shape, only thin layer of hyaline cartilage at the ventral site, reliable bone marrow aspiration from only 10 mm intraosseous depth), sternebra 5 appeared most suitable for bone marrow aspiration. Cultivation and expansion of BM-MSCs was most efficient using fetal calf serum.
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Publication Date: 2016-06-20 PubMed ID: 29067195PubMed Central: PMC5645869DOI: 10.1002/vms3.36Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study explores the optimal techniques to harvest and cultivate multipotent mesenchymal stromal cells (MSCs) from equine sternal bone marrow for regenerative therapies. The study compared aspiration from different sites and tested different culture media.

Research Context and Methodology

  • The researchers noted the growing use of regenerative therapies particularly those involving bone marrow-derived multipotent mesenchymal stromal cells (BM-MSCs) in horses.
  • The successful use of these therapies depends on the efficient harvest and cultivation of these cells, hence the need to optimize techniques and protocols.
  • In this study, they carried out a comparative investigation on bone marrow aspirates from two different sternebrae (bones in the sternum) of horses. This was done at two different positions within the sternebrae, either from 10 mm or from 30 mm dorsal (toward the back) from the ventral (underneath) margin of the sternebrae.
  • They also cross-checked the accuracy of the puncture depth through ultrasounds and computed tomography.
  • They tested three different types of culture media for cultivating the BM-MSCs – Fetal calf serum, standardized horse serum, and autologous serum.

Research Findings and Conclusions

  • The research found that Sternebra 5 was particularly suitable for bone marrow aspiration based on its specific morphological characteristics. It was spherical in shape, had only a thin layer of hyaline cartilage at the ventral site, and the bone marrow could be reliably aspirated from only a 10 mm intraosseous depth.
  • Regarding the culture media, the study concluded that the cultivation and expansion of BM-MSCs was most effective using the fetal calf serum.
  • This research is useful to horse breeders, veterinarians, and scientists involved in equine research and therapy who can now use the findings to optimize their techniques for bone marrow aspiration and cell cultivation for regenerative therapies.

Cite This Article

APA
Eydt C, Geburek F, Schröck C, Hambruch N, Rohn K, Pfarrer C, Staszyk C. (2016). Sternal bone marrow derived equine multipotent mesenchymal stromal cells (MSCs): investigations considering the sampling site and the use of different culture media. Vet Med Sci, 2(3), 200-210. https://doi.org/10.1002/vms3.36

Publication

Veterinary medicine and science
ISSN: 2053-1095
NlmUniqueID: 101678837
Country: England
Language: English
Volume: 2
Issue: 3
Pages: 200-210

Researcher Affiliations

Eydt, Carina
  • Institute of AnatomyUniversity of Veterinary Medicine Hannover, FoundationBischofsholer Damm 15HannoverD-30173Germany.
Geburek, Florian
  • Equine ClinicUniversity of Veterinary Medicine Hannover, FoundationBünteweg 9HannoverD-30559Germany.
Schröck, Carmen
  • Institute of Veterinary-Anatomy-Histology and -EmbryologyFaculty of Veterinary MedicineJustus-Liebig-University GiessenFrankfurter Str. 98GiessenD-35392Germany.
Hambruch, Nina
  • Institute of AnatomyUniversity of Veterinary Medicine Hannover, FoundationBischofsholer Damm 15HannoverD-30173Germany.
Rohn, Karl
  • Institute of Biometry and Information ProcessingUniversity of Veterinary Medicine Hannover, FoundationBünteweg 2HannoverD-30559Germany.
Pfarrer, Christiane
  • Institute of AnatomyUniversity of Veterinary Medicine Hannover, FoundationBischofsholer Damm 15HannoverD-30173Germany.
Staszyk, Carsten
  • Institute of Veterinary-Anatomy-Histology and -EmbryologyFaculty of Veterinary MedicineJustus-Liebig-University GiessenFrankfurter Str. 98GiessenD-35392Germany.

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Citations

This article has been cited 2 times.
  1. Pilgrim CR, McCahill KA, Rops JG, Dufour JM, Russell KA, Koch TG. A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine. Front Vet Sci 2022;9:859025.
    doi: 10.3389/fvets.2022.859025pubmed: 35591873google scholar: lookup
  2. Wen S, Huang X, Ma J, Zhao G, Ma T, Chen K, Huang G, Chen J, Shi J, Wang S. Exosomes derived from MSC as drug system in osteoarthritis therapy. Front Bioeng Biotechnol 2024;12:1331218.
    doi: 10.3389/fbioe.2024.1331218pubmed: 38576449google scholar: lookup
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