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Home / Equine Research Bank / Animals (Basel) / Supplemental Nicotinic Acid Elevates NAD+ Precursors in the ...
Animals : an open access journal from MDPI2022; 12(11); 1383; doi: 10.3390/ani12111383

Supplemental Nicotinic Acid Elevates NAD+ Precursors in the Follicular Fluid of Mares.

Abstract: A deficiency in NAD has previously been linked with increased occurrences of congenital abnormalities and embryonic death in humans and mice. Early embryonic death is a major factor involved in pregnancy loss in mares, and very little is known regarding the NAD requirements for optimum reproductive function in horses. The aim of this study was to determine the effect of supplementing the diet of mares with nicotinic acid (NA) on the composition of NAD metabolites in the blood and follicular fluid. Vehicle alone or NA (3 g per os) were administered to seven mares over a minimum of 3 consecutive days during the follicular phase of the oestrous cycle. Blood samples were collected immediately prior to supplemental feeding and follicular fluid aspiration. Follicular fluid was collected from the dominant follicle through transvaginal ultrasound-guided aspiration. Blood and follicular fluid samples were processed and analysed by mass spectrometry. The concentration of nicotinamide mononucleotide (NMN) in the follicular fluid of NA-fed mares was 4-fold greater than that in the corresponding plasma and 10-fold greater than that in the follicular fluid of vehicle-fed mares. The concentrations of NA, nicotinamide (NAM) and nicotinuric acid (NUR) tended to be greater in the follicular fluid of NA-supplemented mares than in the corresponding plasma. The results show that NA supplementation increased the bioavailability of NAD precursors in the follicular fluid of the dominant follicle, which is proposed to better promote the maturation of good quality oocytes, especially in older mares.
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Publication Date: 2022-05-27 PubMed ID: 35681847PubMed Central: PMC9179367DOI: 10.3390/ani12111383Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the impact of dietary nicotinic acid (NA) supplements on the composition of NAD metabolites in the blood and follicular fluid of mares. Significant findings include that NA supplements increased the bioavailability of NAD precursors in the dominant follicle’s follicular fluid, potentially promoting the maturation of high-quality oocytes, particularly in older mares.

Purpose of Research and Study Design

  • The researchers aimed to understand the influence of adding nicotinic acid (NA) as a supplement to the diet of horses on the NAD metabolites composition within their blood and follicular fluid.
  • This focus arises from previous research that connected NAD deficiency to congenital abnormalities and embryonic death in humans and mice. As early embryonic death also heavily contributes to equine pregnancy loss, the authors sought to illuminate any possible link to NAD availability.
  • They conducted the study using a sample of seven mares. They administered equal portions of NA to the mares every day for a minimum of three days while the horses were in the follicular phase of the oestrous cycle. For control, some mares were given a vehicle (a substance with no intended therapeutic effect).

Data Collection and Analysis

  • The researchers collected blood samples just before dietary supplements were given to each mare, and also aspirated follicular fluid.
  • They performed transvaginal ultrasound-guided aspiration to collect follicular fluid from the dominant follicle in each mare.
  • Blood and follicular fluid specimens were then processed and analyzed using mass spectrometry.

Findings and Conclusion

  • Significant differences were found in the concentration of nicotinamide mononucleotide (NMN), a crucial NAD precursor, in the follicular fluid of the mares given NA supplements. These mares showcased NMN measurements that were four times higher in their follicular fluid than in their plasma, and ten times higher than measurements from mares given the vehicle.
  • Generally, there was also a higher tendency for the concentrations of NA, nicotinamide (NAM), and nicotinuric acid (NUR) to be elevated in the follicular fluid of mares supplemented with NA.
  • The authors conclude from the results that supplementing mares’ diets with NA can increase the bioavailability of necessary NAD precursors within the dominant follicle’s follicular fluid. This increase, the authors postulate, may be instrumental in promoting the maturation of high-quality oocytes – potentially significantly impacting fertility, particularly in older mares.

Cite This Article

APA
Pollard CL, Gibb Z, Clulow J, Ruiz A, Sheridan A, Bahrami M, Swegen A, Grupen CG. (2022). Supplemental Nicotinic Acid Elevates NAD+ Precursors in the Follicular Fluid of Mares. Animals (Basel), 12(11), 1383. https://doi.org/10.3390/ani12111383

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 11
PII: 1383

Researcher Affiliations

Pollard, Charley-Lea
  • Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW 2570, Australia.
Gibb, Zamira
  • Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW 2308, Australia.
Clulow, Jennifer
  • Scone Equine Hospital, Scone, NSW 2337, Australia.
Ruiz, Agustin
  • Newcastle Equine Rehabilitation and Reproduction Centre, Luskintyre, NSW 2321, Australia.
Sheridan, Alecia
  • Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW 2308, Australia.
Bahrami, Mohammad
  • Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW 2308, Australia.
Swegen, Aleona
  • Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW 2308, Australia.
  • Nuffield Department of Women's and Reproductive Health, University of Oxford, Oxford OX1 2JD, UK.
Grupen, Christopher G
  • Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW 2570, Australia.

Grant Funding

  • LP160100824 / Australian Research Council

Conflict of Interest Statement

The authors have declared no conflict of interest.

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Citations

This article has been cited 1 times.
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