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Home / Equine Research Bank / Mar Drugs / The Cladophora glomerata Enriched by Biosorption Process in ...
Marine drugs2017; 15(12); 385; doi: 10.3390/md15120385

The Cladophora glomerata Enriched by Biosorption Process in Cr(III) Improves Viability, and Reduces Oxidative Stress and Apoptosis in Equine Metabolic Syndrome Derived Adipose Mesenchymal Stromal Stem Cells (ASCs) and Their Extracellular Vesicles (MV’s).

Abstract: This study investigated in vitro effects of freshwater alga water extract enriched during a biosorption process in Cr(III) trivalent chromium and chromium picolinate on adipose-derived mesenchymal stromal stem cells (ASCs) and extracellular microvesicles (MVs) in equine metabolic syndrome-affected horses. Chemical characterisation of natural was performed with special emphasis on: vitamin C, vitamin E, total phenols, fatty acids, free and protein-bound amino acids as well as measured Cr in algal biomass. To examine the influence of water extracts, in vitro viability, oxidative stress factor accumulation, apoptosis, inflammatory response, biogenesis of mitochondria, autophagy in ASCs of EMS and secretory activity manifested by MV release were investigated. For this purpose, various methods of molecular biology and microscopic observations (i.e., immunofluorescence staining, SEM, TEM, FIB observations, mRNA and microRNA expression by RT-qPCR) were applied. The extract of enriched with Cr(III) ions reduced apoptosis and inflammation in ASCs of EMS horses through improvement of mitochondrial dynamics, decreasing of PDK4 expression and reduction of endoplastic reticulum stress. Moreover, it was found, that and Cr(III) induce antioxidative protection coming from enhanced SOD activity Therefore, enriched with Cr(III) ions might become an interesting future therapeutic agent in the pharmacological treatment of EMS horses.
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Publication Date: 2017-12-08 PubMed ID: 29292726PubMed Central: PMC5742845DOI: 10.3390/md15120385Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how extracts from a type of freshwater algae, enriched with trivalent chromium (Cr(III)), can potentially reduce oxidative stress and apoptosis in stem cells derived from fat tissue (ASCs) and their microvesicles (MVs) in horses suffering from equine metabolic syndrome.

Study Details and Methods

  • The aim of this study was to investigate the in vitro effects of a type of freshwater alga known as “Cladophora glomerata” on adipose-derived mesenchymal stromal stem cells (ASCs) and extracellular microvesicles (MVs) in horses suffering from equine metabolic syndrome (EMS).
  • The algae were enriched with trivalent chromium (Cr(III)) during a biosorption process and chromium picolinate was also used.
  • Chemical characterisation of the algae was performed with a focus on vitamins like C and E, total phenols, fatty acids, amino acids, and Cr in the algae biomass.
  • The researchers then assessed the influence of these water extract on various cellular factors such as viability, oxidative stress, apoptosis, inflammatory response, mitochondria biogenesis, autophagy in the ASCs of EMS horses, and the release of MVs.
  • Different molecular biology methods and microscopic observations such as immunofluorescence staining, scanning electron microscopy (SEM), transmission electron microscopy (TEM), focused ion beam (FIB) observations, and expression of mRNA and microRNA by quantitative real-time PCR (RT-qPCR) were used in their analysis.

Findings

  • The researchers found that the extracts of Cladophora glomerata enriched with Cr(III) ions showed promising results such as reduction of apoptosis (programmed cell death) and inflammation in the ASCs of EMS horses.
  • These positive effects were achieved through improvements in the dynamics of the mitochondria, decrease in the expression of pyruvate dehydrogenase lipoamide kinase isozyme 4 (PDK4), and reduction of endoplasmic reticulum stress (cellular stress within the protein folding factory of the cell).
  • Furthermore, it was discovered that the algae and Cr(III) induce antioxidative protection coming from an enhanced Superoxide Dismutase (SOD) activity which is an enzyme that helps break down potentially harmful oxygen molecules in cells.

Conclusion

  • Therefore, based on these results, the study suggests that Cladophora glomerata algae enriched with Cr(III) ions may be used as a future therapeutic agent in the treatment of EMS in horses.

Cite This Article

APA
Marycz K, Michalak I, Kocherova I, Marędziak M, Weiss C. (2017). The Cladophora glomerata Enriched by Biosorption Process in Cr(III) Improves Viability, and Reduces Oxidative Stress and Apoptosis in Equine Metabolic Syndrome Derived Adipose Mesenchymal Stromal Stem Cells (ASCs) and Their Extracellular Vesicles (MV’s). Mar Drugs, 15(12), 385. https://doi.org/10.3390/md15120385

Publication

Marine drugs
ISSN: 1660-3397
NlmUniqueID: 101213729
Country: Switzerland
Language: English
Volume: 15
Issue: 12
PII: 385

Researcher Affiliations

Marycz, Krzysztof
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38 C, 50-630 Wroclaw, Poland. krzysztof.marycz@upwr.edu.pl.
  • Wroclawskie Ctr Badan EIT, Stabłowicka 147 St, 54-066 Wroclaw, Poland. krzysztof.marycz@upwr.edu.pl.
Michalak, Izabela
  • Department of Advanced Material Technologies, Faculty of Chemistry, Wrocław University of Science and Technology, Smoluchowskiego 25 St, 50-372 Wrocław, Poland. izabela.michalak@pwr.edu.pl.
Kocherova, Ievgeniia
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38 C, 50-630 Wroclaw, Poland. kocherova.evgenia@gmail.com.
Marędziak, Monika
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38 C, 50-630 Wroclaw, Poland. monika.maredziak@upwr.edu.pl.
Weiss, Christine
  • PferdePraxis Dr. Med. Vet. Daniel Weiss, Postmatte 14, CH-8807 Freienbach, Switzerland. d.weiss@horsedoc.ch.

MeSH Terms

  • Adipose Tissue / cytology
  • Adsorption
  • Animals
  • Antioxidants / pharmacology
  • Apoptosis / drug effects
  • Autophagy / drug effects
  • Cell Survival / drug effects
  • Chlorophyta / chemistry
  • Chromium / chemistry
  • Chromium / pharmacology
  • Extracellular Vesicles / drug effects
  • Extracellular Vesicles / physiology
  • Female
  • Horses
  • Male
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / physiology
  • Metabolic Syndrome / drug therapy
  • Metabolic Syndrome / pathology
  • Metabolic Syndrome / veterinary
  • Mitochondria / drug effects
  • Mitochondria / physiology
  • Mitochondrial Dynamics
  • Oxidative Stress / drug effects
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Primary Cell Culture

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 42 references
  1. Frank N, Geor RJ, Bailey SR, Durham AE, Johnson PJ. Equine metabolic syndrome.. J Vet Intern Med 2010 May-Jun;24(3):467-75.
    doi: 10.1111/j.1939-1676.2010.0503.xpubmed: 20384947google scholar: lookup
  2. Morgan R, Keen J, McGowan C. Equine metabolic syndrome.. Vet Rec 2015 Aug 15;177(7):173-9.
    doi: 10.1136/vr.103226pmc: PMC4552932pubmed: 26273009google scholar: lookup
  3. Geor RJ. Metabolic Predispositions to Laminitis in Horses and Ponies: Obesity, Insulin Resistance and Metabolic Syndromes. J. Equine Vet. Sci. 2008;28:753–759.
    doi: 10.1016/j.jevs.2008.10.016google scholar: lookup
  4. Geor RJ, McCue ME, Schultz N. Current understanding of the equine metabolic syndrome phenotype. J. Equine Vet. Sci. 2013;33:841–844.
    doi: 10.1016/j.jevs.2013.08.010google scholar: lookup
  5. Lopes HF, Corrêa-Giannella ML, Consolim-Colombo FM, Egan BM. Visceral adiposity syndrome.. Diabetol Metab Syndr 2016;8:40.
    doi: 10.1186/s13098-016-0156-2pmc: PMC4950710pubmed: 27437032google scholar: lookup
  6. Hirabara SM, Gorjão R, Vinolo MA, Rodrigues AC, Nachbar RT, Curi R. Molecular targets related to inflammation and insulin resistance and potential interventions.. J Biomed Biotechnol 2012;2012:379024.
    doi: 10.1155/2012/379024pmc: PMC3463198pubmed: 23049242google scholar: lookup
  7. Johnson AR, Milner JJ, Makowski L. The inflammation highway: metabolism accelerates inflammatory traffic in obesity.. Immunol Rev 2012 Sep;249(1):218-38.
    doi: 10.1111/j.1600-065X.2012.01151.xpmc: PMC3422768pubmed: 22889225google scholar: lookup
  8. Marycz K, Grzesiak J, Wrzeszcz K, Golonka P. Adipose stem cell combined with plasma-based implant bone tissue differentiation in vitro and in a horse with a phalanx digitalis distalis fracture: A case report. Vet. Med. Czech Repub. 2012;57:610–617.
  9. Al-Suhaimi EA, Shehzad A. Leptin, resistin and visfatin: the missing link between endocrine metabolic disorders and immunity.. Eur J Med Res 2013 May 1;18(1):12.
    doi: 10.1186/2047-783X-18-12pmc: PMC3655867pubmed: 23634778google scholar: lookup
  10. Makki K, Froguel P, Wolowczuk I. Adipose tissue in obesity-related inflammation and insulin resistance: cells, cytokines, and chemokines.. ISRN Inflamm 2013 Dec 22;2013:139239.
    doi: 10.1155/2013/139239pmc: PMC3881510pubmed: 24455420google scholar: lookup
  11. Baraniak PR, McDevitt TC. Stem cell paracrine actions and tissue regeneration.. Regen Med 2010 Jan;5(1):121-43.
    doi: 10.2217/rme.09.74pmc: PMC2833273pubmed: 20017699google scholar: lookup
  12. Cislo-Pakuluk A, Marycz K. A Promising Tool in Retina Regeneration: Current Perspectives and Challenges When Using Mesenchymal Progenitor Stem Cells in Veterinary and Human Ophthalmological Applications.. Stem Cell Rev Rep 2017 Oct;13(5):598-602.
    doi: 10.1007/s12015-017-9750-4pmc: PMC5602072pubmed: 28643176google scholar: lookup
  13. Basinska K, Marycz K, Śieszek A, Nicpoń J. The production and distribution of IL-6 and TNF-a in subcutaneous adipose tissue and their correlation with serum concentrations in Welsh ponies with equine metabolic syndrome.. J Vet Sci 2015;16(1):113-20.
    doi: 10.4142/jvs.2015.16.1.113pmc: PMC4367141pubmed: 25269712google scholar: lookup
  14. Nicpoń J, Marycz K, Grzesiak J. Therapeutic effect of adipose-derived mesenchymal stem cell injection in horses suffering from bone spavin.. Pol J Vet Sci 2013;16(4):753-4.
    doi: 10.2478/pjvs-2013-0107pubmed: 24597313google scholar: lookup
  15. Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A. Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine.. In Vitro Cell Dev Biol Anim 2015 Mar;51(3):230-40.
    doi: 10.1007/s11626-014-9828-0pmc: PMC4368852pubmed: 25428200google scholar: lookup
  16. Marycz K, Kornicka K, Grzesiak J, Śmieszek A, Szłapka J. Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation.. Oxid Med Cell Longev 2016;2016:3718468.
    doi: 10.1155/2016/3718468pmc: PMC5178365pubmed: 28053691google scholar: lookup
  17. Marycz K, Kornicka K, Basinska K, Czyrek A. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging.. Oxid Med Cell Longev 2016;2016:4710326.
    doi: 10.1155/2016/4710326pmc: PMC4670679pubmed: 26682006google scholar: lookup
  18. Hua Y, Clark S, Ren J, Sreejayan N. Molecular mechanisms of chromium in alleviating insulin resistance.. J Nutr Biochem 2012 Apr;23(4):313-9.
    doi: 10.1016/j.jnutbio.2011.11.001pmc: PMC3308119pubmed: 22423897google scholar: lookup
  19. Chameroy KA, Frank N, Elliott SB, Boston RC. Effects of a supplement containing chromium and magnesium on morphometric measurements, resting glucose, insulin concentrations and insulin sensitivity in laminitic obese horses.. Equine Vet J 2011 Jul;43(4):494-9.
    doi: 10.1111/j.2042-3306.2010.00302.xpubmed: 21496075google scholar: lookup
  20. Cao DJ, Shi XD, Li H, Xie PP, Zhang HM, Deng JW, Liang YG. Effects of lead on tolerance, bioaccumulation, and antioxidative defense system of green algae, Cladophora.. Ecotoxicol Environ Saf 2015 Feb;112:231-7.
    doi: 10.1016/j.ecoenv.2014.11.007pubmed: 25463875google scholar: lookup
  21. Berdalet E, Fleming LE, Gowen R, Davidson K, Hess P, Backer LC, Moore SK, Hoagland P, Enevoldsen H. Marine harmful algal blooms, human health and wellbeing: challenges and opportunities in the 21st century.. J Mar Biol Assoc U K 2015;2015.
    doi: 10.1017/S0025315415001733pmc: PMC4676275pubmed: 26692586google scholar: lookup
  22. Shalaby EA. Algae as promising organisms for environment and health.. Plant Signal Behav 2011 Sep;6(9):1338-50.
    doi: 10.4161/psb.6.9.16779pmc: PMC3258063pubmed: 21862867google scholar: lookup
  23. Michalak I, Chojnacka K, Korniewicz D. New feed supplement from macroalgae as the dietary source of microelements for pigs. Open Chem. 2016;13.
    doi: 10.1515/chem-2015-0149google scholar: lookup
  24. Michalak I, Chojnacka K, Dobrzański Z, Górecki H, Zielińska A, Korczyński M, Opaliński S. Effect of macroalgae enriched with microelements on egg quality parameters and mineral content of eggs, eggshell, blood, feathers and droppings.. J Anim Physiol Anim Nutr (Berl) 2011 Jun;95(3):374-87.
    doi: 10.1111/j.1439-0396.2010.01065.xpubmed: 21039928google scholar: lookup
  25. Lindåse S, Nostell K, Bröjer J. A modified oral sugar test for evaluation of insulin and glucose dynamics in horses.. Acta Vet Scand 2016 Oct 20;58(Suppl 1):64.
    doi: 10.1186/s13028-016-0246-zpmc: PMC5073908pubmed: 27766982google scholar: lookup
  26. Durham AE. Therapeutics for Equine Endocrine Disorders.. Vet Clin North Am Equine Pract 2017 Apr;33(1):127-139.
    doi: 10.1016/j.cveq.2016.11.003pubmed: 28190613google scholar: lookup
  27. Michalak I, Chojnacka K, Saeid A. Plant Growth Biostimulants, Dietary Feed Supplements and Cosmetics Formulated with Supercritical CO₂ Algal Extracts.. Molecules 2017 Jan 3;22(1).
    doi: 10.3390/molecules22010066pmc: PMC6155630pubmed: 28054954google scholar: lookup
  28. Michalak I, Miller U, Tuhy Ł, Sówka I, Chojnacka K. Characterisation of biological properties of co-composted Baltic seaweeds in germination tests. Eng. Life Sci. 2017;17:153–164.
    doi: 10.1002/elsc.201600012google scholar: lookup
  29. Belay A, Ota Y, Miyakawa K, Shimamatsu H. Current knowledge on potential health benefits of Spirulina. J. Appl. Phycol. 1993;5:235–241.
    doi: 10.1007/BF00004024google scholar: lookup
  30. Nawrocka D, Kornicka K, Śmieszek A, Marycz K. Spirulina platensis Improves Mitochondrial Function Impaired by Elevated Oxidative Stress in Adipose-Derived Mesenchymal Stromal Cells (ASCs) and Intestinal Epithelial Cells (IECs), and Enhances Insulin Sensitivity in Equine Metabolic Syndrome (EMS) Horses.. Mar Drugs 2017 Aug 3;15(8).
    doi: 10.3390/md15080237pmc: PMC5577592pubmed: 28771165google scholar: lookup
  31. Hu S, Wang D, Zhang J, Du M, Cheng Y, Liu Y, Zhang N, Wang D, Wu Y. Mitochondria Related Pathway Is Essential for Polysaccharides Purified from Sparassis crispa Mediated Neuro-Protection against Glutamate-Induced Toxicity in Differentiated PC12 Cells.. Int J Mol Sci 2016 Jan 26;17(2).
    doi: 10.3390/ijms17020133pmc: PMC4783876pubmed: 26821016google scholar: lookup
  32. Subramoniam A, Asha VV, Nair SA, Sasidharan SP, Sureshkumar PK, Rajendran KN, Karunagaran D, Ramalingam K. Chlorophyll revisited: anti-inflammatory activities of chlorophyll a and inhibition of expression of TNF-α gene by the same.. Inflammation 2012 Jun;35(3):959-66.
    doi: 10.1007/s10753-011-9399-0pubmed: 22038065google scholar: lookup
  33. Liu Q, Huang Y, Zhang R, Cai T, Cai Y. Medical Application of Spirulina platensis Derived C-Phycocyanin.. Evid Based Complement Alternat Med 2016;2016:7803846.
    doi: 10.1155/2016/7803846pmc: PMC4879233pubmed: 27293463google scholar: lookup
  34. Zhou J, Xu H, Huang K. Organoselenium Small Molecules and Chromium(III) Complexes for Intervention in Chronic Low-grade Inflammation and Type 2 Diabetes.. Curr Top Med Chem 2016;16(8):823-34.
    doi: 10.2174/1568026615666150827094815pubmed: 26311428google scholar: lookup
  35. Patlolla AK, Barnes C, Yedjou C, Velma VR, Tchounwou PB. Oxidative stress, DNA damage, and antioxidant enzyme activity induced by hexavalent chromium in Sprague-Dawley rats.. Environ Toxicol 2009 Feb;24(1):66-73.
    doi: 10.1002/tox.20395pmc: PMC2769560pubmed: 18508361google scholar: lookup
  36. Jeoung NH, Harris RA. Pyruvate dehydrogenase kinase-4 deficiency lowers blood glucose and improves glucose tolerance in diet-induced obese mice.. Am J Physiol Endocrinol Metab 2008 Jul;295(1):E46-54.
    doi: 10.1152/ajpendo.00536.2007pmc: PMC2493588pubmed: 18430968google scholar: lookup
  37. Lee IK. The role of pyruvate dehydrogenase kinase in diabetes and obesity.. Diabetes Metab J 2014 Jun;38(3):181-6.
    doi: 10.4093/dmj.2014.38.3.181pmc: PMC4083023pubmed: 25003070google scholar: lookup
  38. Shenouda SM, Widlansky ME, Chen K, Xu G, Holbrook M, Tabit CE, Hamburg NM, Frame AA, Caiano TL, Kluge MA, Duess MA, Levit A, Kim B, Hartman ML, Joseph L, Shirihai OS, Vita JA. Altered mitochondrial dynamics contributes to endothelial dysfunction in diabetes mellitus.. Circulation 2011 Jul 26;124(4):444-53.
    doi: 10.1161/CIRCULATIONAHA.110.014506pmc: PMC3149100pubmed: 21747057google scholar: lookup
  39. Yu T, Robotham JL, Yoon Y. Increased production of reactive oxygen species in hyperglycemic conditions requires dynamic change of mitochondrial morphology.. Proc Natl Acad Sci U S A 2006 Feb 21;103(8):2653-8.
    doi: 10.1073/pnas.0511154103pmc: PMC1413838pubmed: 16477035google scholar: lookup
  40. Michalak I, Chojnacka K. The new application of biosorption properties of Enteromorpha prolifera.. Appl Biochem Biotechnol 2010 Mar;160(5):1540-56.
    doi: 10.1007/s12010-009-8635-7pubmed: 19404780google scholar: lookup
  41. Davidson I. Hydrolysis of samples for amino acid analysis.. Methods Mol Biol 2003;211:111-22.
    pubmed: 12489425doi: 10.1385/1-59259-342-9:111google scholar: lookup
  42. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.. Anal Biochem 1987 Apr;162(1):156-9.
    doi: 10.1016/0003-2697(87)90021-2pubmed: 2440339google scholar: lookup

Citations

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  1. Bourebaba L, Zyzak M, Sikora M, Serwotka-Suszczak A, Mularczyk M, Al Naem M, Marycz K. Sex Hormone-Binding Globulin (SHBG) Maintains Proper Equine Adipose-Derived Stromal Cells (ASCs)' Metabolic Functions and Negatively Regulates their Basal Adipogenic Potential. Stem Cell Rev Rep 2023 Jul 4;.
    doi: 10.1007/s12015-023-10580-8pubmed: 37402098google scholar: lookup
  2. Aldbass A, Amina M, Al Musayeib NM, Bhat RS, Al-Rashed S, Marraiki N, Fahmy R, El-Ansary A. Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays. Sci Rep 2021 Apr 19;11(1):8512.
    doi: 10.1038/s41598-021-88089-8pubmed: 33875747google scholar: lookup
  3. Bourebaba L, Michalak I, Baouche M, Kucharczyk K, Fal AM, Marycz K. Cladophora glomerata enriched by biosorption with Mn(II) ions alleviates lipopolysaccharide-induced osteomyelitis-like model in MC3T3-E1, and 4B12 osteoclastogenesis. J Cell Mol Med 2020 Jul;24(13):7282-7300.
    doi: 10.1111/jcmm.15294pubmed: 32497406google scholar: lookup
  4. Kornicka-Garbowska K, Pędziwiatr R, Woźniak P, Kucharczyk K, Marycz K. Microvesicles isolated from 5-azacytidine-and-resveratrol-treated mesenchymal stem cells for the treatment of suspensory ligament injury in horse-a case report. Stem Cell Res Ther 2019 Dec 18;10(1):394.
    doi: 10.1186/s13287-019-1469-5pubmed: 31852535google scholar: lookup
  5. Bourebaba L, Michalak I, Baouche M, Kucharczyk K, Marycz K. Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome. Stem Cell Res Ther 2019 Dec 17;10(1):392.
    doi: 10.1186/s13287-019-1499-zpubmed: 31847882google scholar: lookup
  6. Wang YH, Guo YC, Wang DR, Liu JY, Pan J. Adipose Stem Cell-Based Clinical Strategy for Neural Regeneration: A Review of Current Opinion. Stem Cells Int 2019;2019:8502370.
    doi: 10.1155/2019/8502370pubmed: 31827536google scholar: lookup
  7. Al Naem M, Bourebaba L, Kucharczyk K, Röcken M, Marycz K. Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders. Stem Cell Rev Rep 2020 Apr;16(2):301-322.
    doi: 10.1007/s12015-019-09932-0pubmed: 31797146google scholar: lookup
  8. Bourebaba L, Bedjou F, Röcken M, Marycz K. Nortropane alkaloids as pharmacological chaperones in the rescue of equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome through mitochondrial potentiation, endoplasmic reticulum stress mitigation and insulin resistance alleviation. Stem Cell Res Ther 2019 Jun 18;10(1):178.
    doi: 10.1186/s13287-019-1292-zpubmed: 31215461google scholar: lookup
  9. Marycz K, Houston JMI, Weiss C, Röcken M, Kornicka K. 5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy. Oxid Med Cell Longev 2019;2019:1523140.
    doi: 10.1155/2019/1523140pubmed: 31214275google scholar: lookup
  10. Smieszek A, Kornicka K, Szłapka-Kosarzewska J, Androvic P, Valihrach L, Langerova L, Rohlova E, Kubista M, Marycz K. Metformin Increases Proliferative Activity and Viability of Multipotent Stromal Stem Cells Isolated from Adipose Tissue Derived from Horses with Equine Metabolic Syndrome. Cells 2019 Jan 22;8(2).
    doi: 10.3390/cells8020080pubmed: 30678275google scholar: lookup
  11. Michalak I, Mironiuk M, Marycz K. A comprehensive analysis of biosorption of metal ions by macroalgae using ICP-OES, SEM-EDX and FTIR techniques. PLoS One 2018;13(10):e0205590.
    doi: 10.1371/journal.pone.0205590pubmed: 30321205google scholar: lookup
  12. Kornicka K, Houston J, Marycz K. Dysfunction of Mesenchymal Stem Cells Isolated from Metabolic Syndrome and Type 2 Diabetic Patients as Result of Oxidative Stress and Autophagy may Limit Their Potential Therapeutic Use. Stem Cell Rev Rep 2018 Jun;14(3):337-345.
    doi: 10.1007/s12015-018-9809-xpubmed: 29611042google scholar: lookup
  13. Bourebaba N, Domagała J, Bourebaba L. Revitalizing equine metabolism: how SHBG improves mitochondrial function and reduces inflammation. BMC Vet Res 2025 Oct 21;21(1):620.
    doi: 10.1186/s12917-025-05033-ypubmed: 41121214google scholar: lookup
  14. Yao X, Wan X, Chi Y, Nie X. Preparation, structure, and biological activity of sulfated polysaccharides from green algae in the Cladophoraceae (Cladophorales, Ulvophyceae). Antonie Van Leeuwenhoek 2025 Aug 15;118(9):131.
    doi: 10.1007/s10482-025-02146-0pubmed: 40815337google scholar: lookup
  15. El Masri J, Fadlallah H, Al Sabsabi R, Afyouni A, Al-Sayegh M, Abou-Kheir W. Adipose-Derived Stem Cell Therapy in Spinal Cord Injury. Cells 2024 Sep 9;13(17).
    doi: 10.3390/cells13171505pubmed: 39273075google scholar: lookup
  16. Lanci A, Iacono E, Merlo B. Therapeutic Application of Extracellular Vesicles Derived from Mesenchymal Stem Cells in Domestic Animals. Animals (Basel) 2024 Jul 24;14(15).
    doi: 10.3390/ani14152147pubmed: 39123673google scholar: lookup
  17. Tomal A, Szłapka-Kosarzewska J, Mironiuk M, Michalak I, Marycz K. Arthrospira platensis enriched with Cr(III), Mg(II), and Mn(II) ions improves insulin sensitivity and reduces systemic inflammation in equine metabolic affected horses. Front Endocrinol (Lausanne) 2024;15:1382844.
    doi: 10.3389/fendo.2024.1382844pubmed: 38689728google scholar: lookup
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