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Home / Equine Research Bank / Animals (Basel) / The Dynamics of Circulating Immune Complexes in Horses with ...
Animals : an open access journal from MDPI2021; 11(4); 1001; doi: 10.3390/ani11041001

The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma.

Abstract: Non-invasive diagnostic biomarkers of equine asthma syndrome (EAS) from blood or urine are sought. The aim of this study was to assess the absorbance of circulating immune complexes (CICs) during the exacerbation, remission, and treatment of an asthma episode and assess the potential usefulness of CIC levels in the diagnosis and monitoring of the disease. The control group, asthma group, and treated asthma group each contained six horses. Following an initial examination and group classification, the horses were kept in a dusty environment for seven days and then moved to an asthma-friendly environment for three weeks (the treated group received injections of glucocorticoids). Blood was collected at baseline and on the 1st, 2nd, 3rd, 7th, 14th and 30th days. CIC was measured using the modified Haskova method. The time points did not show significant statistical differences. There was a significant decrease in CIC in the treated group, and a significant increase in CIC in the non-treated group on day 30. CIC did not support the EAS diagnosis, although it may help in monitoring patients. To the best of the authors' knowledge, this is the first study to analyse the dynamics of CIC during environmental challenge, remission, and treatment.
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Publication Date: 2021-04-02 PubMed ID: 33918401PubMed Central: PMC8066133DOI: 10.3390/ani11041001Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aimed to analyze the levels of circulating immune complexes (CICs) in horses suffering from severe equine asthma under various conditions in order to assess the potential utility of CIC levels as a diagnostic and monitoring tool for the disease.

Study Design and Methodology

  • The study comprised three groups of horses – a control group, an asthma group, and a treated asthma group, each containing six horses.
  • Post initial examination and group classification, all three groups of horses were subjected to a dusty environment for a week which was followed by shift to an asthma-friendly environment for three weeks. During this period, the treated group was administered injections of glucocorticoids.
  • Blood samples were collected at baseline and then repeatedly on the 1st, 2nd, 3rd, 7th, 14th and 30th days.
  • Circulating immune complexes (CICs) in these blood samples were measured using a modified Haskova method.

Study Findings

  • The study did not show any significant statistical differences at different time points.
  • On the 30th day, a significant decrease was observed in the levels of CIC in the treated group, whereas an increase was found in the non-treated group.
  • The observation did not support the assertion that measurement of CICs could be used as a tool for diagnosing EAS. However, the data suggested a role in the monitoring of the disease condition in horses.

Significance

  • This is the first study of its kind to investigate the dynamics of circulation immune complexes during the phases of environmental challenge, remission, and treatment in severe equine asthma.
  • The analysis offers hints towards the potential role of CICs as a monitoring tool for severe equine asthma, but further investigation is necessary to validate these findings.

Cite This Article

APA
Slowikowska M, Bajzert J, Miller J, Stefaniak T, Niedzwiedz A. (2021). The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma. Animals (Basel), 11(4), 1001. https://doi.org/10.3390/ani11041001

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 4
PII: 1001

Researcher Affiliations

Slowikowska, Malwina
  • Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland.
Bajzert, Joanna
  • Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
Miller, Julia
  • Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
Stefaniak, Tadeusz
  • Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
Niedzwiedz, Artur
  • Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland.

Grant Funding

  • B030/0056/17 / Uniwersytet Przyrodniczy we Wroclawiu

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Citations

This article has been cited 2 times.
  1. Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.. Animals (Basel) 2022 Mar 16;12(6).
    doi: 10.3390/ani12060744pubmed: 35327141google scholar: lookup
  2. Borowska A, Wolska D, Niedzwiedz A, Borowicz H, Jaworski Z, Siemieniuch M, Szwaczkowski T. Some Genetic and Environmental Effects on Equine Asthma in Polish Konik Horses.. Animals (Basel) 2021 Aug 3;11(8).
    doi: 10.3390/ani11082285pubmed: 34438743google scholar: lookup
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