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Home / Equine Research Bank / Front Vet Sci / The Effect of Autologous Protein Solution on the Inflammator...
Frontiers in veterinary science2019; 6; 64; doi: 10.3389/fvets.2019.00064

The Effect of Autologous Protein Solution on the Inflammatory Cascade in Stimulated Equine Chondrocytes.

Abstract: Cartilage injury occurs commonly in equine athletes, often precipitating posttraumatic osteoarthritis (PTOA). Orthobiologics such as autologous conditioned serum (ACS) and autologous protein solution (APS) may be useful in decreasing posttraumatic inflammation, thereby preventing PTOA. The objective of this study was to quantify cytokine concentrations in ACS and APS and evaluate the protective effects of ACS and APS on inflamed chondrocytes cultured . We hypothesized that the combination of platelet-derived growth factors (PDGF) and anti-inflammatory cytokines present in APS would be superior in decreasing the inflammatory and catabolic cascade in inflamed chondrocytes when compared to ACS in which platelets are excluded from the preparation. Chondrocytes were isolated from the cartilage of femoral trochlear ridges of 6 horses and cultured in 12-well transwell plates. Treatment groups included: (1) control, (2) APS (Pro-Stride; Owl Manor), and (3) ACS (IRAP II; Arthrex). Each group was unstimulated or stimulated with IL-1β and TNF-α for 48 h. The concentration of IL-1β, IL-6, TNF-α, MMP-3, MMP-13, and IL-10 was quantified using a fluorescent bead-based multiplex assay. IL-1Ra concentration was quantified using ELISA. APS and ACS both had significantly increased concentrations of IL-1Ra without a concurrent increase in IL-1β concentration. After 48 h of culture, media from chondrocytes treated with APS contained significantly increased concentrations of IL-1Ra and IL-10. APS-treated cultures had increased concentrations of IL-6. Overall, APS effectively concentrated IL-1Ra without an incubation period and media from APS-treated chondrocytes had increased concentrations of chondroprotective (IL-1Ra and IL-10) and modulatory (IL-6) cytokines, which may be beneficial in the treatment of inflammatory conditions such as PTOA.
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Publication Date: 2019-03-06 PubMed ID: 30895181PubMed Central: PMC6414419DOI: 10.3389/fvets.2019.00064Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper presents a study on the impact of using autologous protein solution (APS) and autologous conditioned serum (ACS) on induced inflammation in horse cartilage cells. The study concludes that APS concentrated protective cytokines without needing incubation and led to increased levels of chondroprotective and modulatory cytokines, which could be beneficial in treating inflammatory conditions like posttraumatic osteoarthritis.

Objective of the Research

  • The research aimed to quantify cytokine concentrations in APS and ACS and evaluate their protective effects on inflamed chondrocytes (cartilage cells).
  • The researchers hypothesized that the combination of platelet-derived growth factors (PDGF) and anti-inflammatory cytokines present in APS would be superior to the ACS in alleviating inflammation in chondrocytes.

The Study Procedure

  • The study was conducted by isolating chondrocytes from the cartilage of femoral trochlear ridges of 6 horses and cultivating them in 12-well transwell plates.
  • The treatment groups included a control group, a group treated with APS, and a group treated with ACS.
  • The researchers either left the groups alone or stimulated them with the pro-inflammatory cytokines IL-1β and TNF-α for 48 hours.
  • After the treatment period, the researchers quantified the concentrations of several cytokines using a fluorescent bead-based multiplex assay and an ELISA.

Key Findings

  • Both APS and ACS showed significantly increased concentrations of the anti-inflammatory cytokine IL-1Ra, without an increase in the inflammatory cytokine IL-1β concentration.
  • The media from chondrocytes treated with APS showed significantly increased concentrations of IL-1Ra and another anti-inflammatory cytokine IL-10.
  • APS-treated cultures demonstrated increased concentrations of the modulatory cytokine IL-6.

Conclusion of the Research

  • APS can effectively concentrate the anti-inflammatory cytokine IL-1Ra without an incubation period, increasing its potential utility in treating inflammatory conditions.
  • The media from APS-treated chondrocytes had higher concentrations of chondroprotective (IL-1Ra and IL-10) and modulatory (IL-6) cytokines, which could be beneficial for treating posttraumatic osteoarthritis.

Cite This Article

APA
Linardi RL, Dodson ME, Moss KL, King WJ, Ortved KF. (2019). The Effect of Autologous Protein Solution on the Inflammatory Cascade in Stimulated Equine Chondrocytes. Front Vet Sci, 6, 64. https://doi.org/10.3389/fvets.2019.00064

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 6
Pages: 64
PII: 64

Researcher Affiliations

Linardi, Renata L
  • Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA, United States.
Dodson, Michael E
  • Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA, United States.
Moss, Kaitlyn L
  • Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA, United States.
King, William J
  • Owl Manor Veterinary Inc., Warsaw, IN, United States.
Ortved, Kyla F
  • Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA, United States.

Grant Funding

  • T35 OD010919 / NIH HHS

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This article has been cited 22 times.
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