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Journal of veterinary science2014; 16(1); 113-120; doi: 10.4142/jvs.2015.16.1.113

The production and distribution of IL-6 and TNF-a in subcutaneous adipose tissue and their correlation with serum concentrations in Welsh ponies with equine metabolic syndrome.

Abstract: A main symptom of equine metabolic syndrome (EMS) in ponies is pathological obesity characterized by abnormal accumulation of fat deposits and inflammation. In this study, we analyzed the expression of two pro-inflammatory cytokines, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), in subcutaneous adipose tissue and the correlation with serum concentrations in peripheral blood of Welsh ponies. Based on clinical examination findings, the animals were divided into two groups: ponies affected with EMS (n = 8) and obese ponies (n = 8). The adipose tissue was examined using immunohistochemical analysis while concentrations IL-6 and TNF-a were measured using enzyme-linked immunosorbent assays (ELISAs). Additionally, histological characterization of the adipose tissue was performed. The results obtained showed that IL-6 expression in adipose tissue biopsies derived from animals with EMS was enhanced while TNF-α levels of both groups were comparable. Compared to the obese ponies, EMS animals also had significantly elevated levels of serum IL-6 and TNF-α. Histological analysis revealed macrophage infiltration and fibrosis in adipose tissue preparations from the EMS group. These data suggest that IL-6 may play a key role in the course of EMS in Welsh ponies. Our findings also demonstrated that analysis of pro-inflammatory cytokines levels in serum may serve as an additional tool for diagnosing EMS.
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Publication Date: 2014-09-30 PubMed ID: 25269712PubMed Central: PMC4367141DOI: 10.4142/jvs.2015.16.1.113Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the role of two inflammation-related proteins in equine metabolic syndrome (EMS), a condition similar to metabolic syndrome in humans, in Welsh ponies. The findings suggest that measuring these proteins might help diagnose this health problem.

Objective of the Study

The study aimed to determine the production of two pro-inflammatory cytokines, IL-6 and TNF-α, in the adipose (fat) tissue of Welsh ponies with equine metabolic syndrome (EMS) and analyze their correlation with the serum levels of these proteins. Additionally, the research assessed the role of these cytokines in EMS development.

  • Obese ponies and ponies affected by EMS were the two groups of animals used in the study.
  • The histological characterization of fat tissue and immunohistochemical analysis for cytokines localization were performed.
  • The serum levels of these cytokines were determined using enzyme-linked immunosorbent assays (ELISAs), a common laboratory technique for detecting proteins.

Main Findings

The findings of the study provide a clear understanding of the health impacts of EMS in ponies, primarily focusing on IL-6 and TNF-α proteins.

  • IL-6 levels were found to be enhanced in the fat tissue biopsies from EMS-affected ponies when compared to obese ponies, while there were no significant differences in the TNF-α levels between both groups.
  • EMS animals also exhibited significantly increased levels of IL-6 and TNF-α in their serum. These elevated serum levels suggest a systematic inflammatory response related to EMS.
  • On examining the histological preparations from the EMS group, macrophage infiltration and fibrosis were noticed in the adipose tissues, indicating a significant inflammatory condition.

Implications of the Study

This research project delivers critical insights into EMS and its associated inflammatory responses.

  • The results highlighted the significant role of IL-6 in the development of EMS, suggesting its potential usage as a target for future therapeutic interventions.
  • The findings also suggested that analyzing the serum levels of pro-inflammatory cytokines can serve as a useful diagnostic tool for EMS, thereby, enhancing early detection and treatment strategies.

Cite This Article

APA
Basinska K, Marycz K, Śieszek A, Nicpoń J. (2014). The production and distribution of IL-6 and TNF-a in subcutaneous adipose tissue and their correlation with serum concentrations in Welsh ponies with equine metabolic syndrome. J Vet Sci, 16(1), 113-120. https://doi.org/10.4142/jvs.2015.16.1.113

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 16
Issue: 1
Pages: 113-120

Researcher Affiliations

Basinska, Katarzyna
  • Electron Microscope Laboratory, Wroclaw University of Environmental and Life Sciences, Wroclaw 51-631, Poland.basinskakatarzyna@wp.pl.
Marycz, Krzysztof
    Śieszek, Agnieszka
      Nicpoń, Jakub

        MeSH Terms

        • Adipose Tissue / metabolism
        • Animals
        • Female
        • Horse Diseases / blood
        • Horse Diseases / metabolism
        • Horses
        • Interleukin-6 / blood
        • Interleukin-6 / genetics
        • Interleukin-6 / metabolism
        • Male
        • Metabolic Syndrome / metabolism
        • Metabolic Syndrome / veterinary
        • Tumor Necrosis Factor-alpha / blood
        • Tumor Necrosis Factor-alpha / genetics
        • Tumor Necrosis Factor-alpha / metabolism

        Conflict of Interest Statement

        There is no conflict of interest.

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