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Home / Equine Research Bank / J Vet Intern Med / Time to resolution of airway inflammation caused by bronchoa...
Journal of veterinary internal medicine2024; doi: 10.1111/jvim.17169

Time to resolution of airway inflammation caused by bronchoalveolar lavage in healthy horses.

Abstract: Bronchoalveolar lavage (BAL) is a common procedure for evaluation of the equine lower airways. Time to resolution of post-BAL inflammation has not been clearly defined. Objective: Residual inflammation, evident by changes in immune cell populations and inflammatory cytokines, will resolve by 72 hours after BAL. Methods: Six adult, healthy, institution-owned horses. Methods: Randomized, complete cross-over design. Each horse underwent 3 paired BALs, including a baseline and then 48, 72, and 96 hours later, with a 7-day washout between paired BALs. Each sample underwent cytological evaluation and cytokine concentrations were determined by a commercially available multiplex bead immunoassay. Statistical analysis was performed by multilevel mixed-effects Poisson regression analysis. Data are reported as marginal means and 95% confidence interval (CI). Results: Neutrophil, eosinophil and mast cell percentages were not significantly different at any time points. Macrophage percentages were higher at 72 hours (45.0 [95% CI, 41.6-48.4]%) and 96 hours (45.3 [95% CI, 42.9-47.7]%) vs baseline (37.4 [95% CI, 33.5-41.4]%; P < .001 and P = .01, respectively), and at 72 hours and 96 hours vs 48 hours (31.9 [95% CI, 28.1-35.6]%; P < .001). Neutrophil percentage was not significantly increased at 48 hours (P = .11). Interleukin (IL)-6 concentration was increased at 72 hours (5.22 [95% CI, 3.44-6.99] pg/mL) vs 48 hours (4.38 [95% CI, 2.99-5.78] pg/mL; P < .001). Conclusions: Significant lung inflammation was not detected at 72 and 96 hours, suggesting that repeating BAL at 72 hours or more can be done without concern of residual inflammation.
© 2024 The Author(s). Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2024-08-28 PubMed ID: 39198933DOI: 10.1111/jvim.17169Google Scholar: Lookup
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  • Journal Article

Summary

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The article examines the time taken for horses’ airways to recover from inflammation caused by a procedure known as bronchoalveolar lavage (BAL). The findings suggest that significant inflammation was not detected 72 and 96 hours after the procedure, indicating that it is safe to perform a repeat BAL after this time without concerns of residual inflammation.

Study Design and Methodology

  • The study was carried out on six healthy, adult, institution-owned horses. Each horse underwent three pairs of BALs (washing out of bronchial tubes), at different intervals: baseline, 48 hours, 72 hours and 96 hours after the procedure, followed by a resting period of seven days between each BAL.
  • The samples collected were analysed for cytological evaluation and cytokine concentrations, the latter of which was determined by a commercially available multiplex bead immunoassay.
  • A comprehensive statistical analysis using multilevel mixed-effects Poisson regression analysis was used to interpret the data. The results are presented as marginal means and 95% confidence intervals (CIs).

Results

  • The percentages of neutrophils, eosinophils, and mast cells did not show any significant change over the different time points.
  • The macrophage percentages were notably higher at the 72nd and the 96th hours (45.0% and 45.3%) when compared to the baseline (37.4%), and also when compared with the percentages at 48 hours (31.9%).
  • There was no noticeable increase in neutrophil percentages after 48 hours.
  • The concentration of a certain cytokine, Interleukin-6 (IL-6), rose at 72 hours (5.22 pg/mL) compared to the level at 48 hours (4.38 pg/mL).

Conclusions

  • The research concluded that significant lung inflammation was not observed 72 and 96 hours post-BAL, and suggested that repeating BAL after 72 hours does not pose a risk of residual inflammation.
  • The increased macrophage percentages and IL-6 concentrations noted at 72 hours may be part of the normal immunological response to BAL and do not indicate pathological inflammation. Hence, it is considered safe for veterinarians to repeat BAL in horses after a minimum of 72 hours post-procedure.
  • This study provides important guidelines for the use of BAL in equine healthcare and research settings, and may contribute to improved procedural safety and animal welfare in the future.

Cite This Article

APA
Woodrow JS, Hopster K, Palmisano M, Payette F, Kulp J, Stefanovski D, Nolen-Walston R. (2024). Time to resolution of airway inflammation caused by bronchoalveolar lavage in healthy horses. J Vet Intern Med. https://doi.org/10.1111/jvim.17169

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English

Researcher Affiliations

Woodrow, Jane S
  • Department of Clinical Sciences-New Bolton Center, College of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.
Hopster, Klaus
  • Department of Clinical Sciences-New Bolton Center, College of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.
Palmisano, Megan
  • Department of Clinical Sciences-New Bolton Center, College of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.
Payette, Flavie
  • Department of Clinical Sciences-New Bolton Center, College of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.
Kulp, Jeaneen
  • Department of Clinical Sciences-New Bolton Center, College of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.
Stefanovski, Darko
  • Department of Clinical Sciences-New Bolton Center, College of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.
Nolen-Walston, Rose
  • Department of Clinical Sciences-New Bolton Center, College of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.

Grant Funding

  • Raymond Firestone Trust Research Grant

References

This article includes 45 references
  1. Von Essen SG, Robbins RA, Spurzem JR. Bronchoscopy with bronchoalveolar lavage causes neutrophil recruitment to the lower respiratory tract.. Am Rev Respir Dis 1991;144:848‐854.
  2. Sweeney CR, Rossier Y, Ziemer EL, Lindborg SR. Effect of prior lavage on bronchoalveolar lavage fluid cell population of lavaged and unlavaged lung segments in horses.. Am J Vet Res 1994;55:1501‐1504.
  3. Westermann CM, de Bie AG, Olave C, de Grauw JC, Teske E, Couetil LL. Effect of lavage solution type on bronchoalveolar lavage fluid cytology in clinically healthy horses.. Animals (Basel) 2023;13:2367.
  4. Orard M, Depecker M, Hue E, Pitel PH, Couroucé‐Malblanc A, Richard EA. Influence of bronchoalveolar lavage volume on cytological profiles and subsequent diagnosis of inflammatory airway disease in horses.. Vet J 2016;207:193‐195.
  5. Hue E, Orard M, Toquet MP. Asymmetrical pulmonary cytokine profiles are linked to bronchoalveolar lavage fluid cytology of horses with mild airway neutrophilia.. Front Vet Sci 2020;7:226.
  6. Derksen FJ, Scott JS, Miller DC, Slocombe RF, Robinson NE. Bronchoalveolar lavage in ponies with recurrent airway obstruction (heaves).. Am Rev Respir Dis 1985;132:1066‐1070.
  7. Clark CK, Lester GD, Vetro T. Bronchoalveolar lavage in horses: effect of exercise and repeated sampling on cytology.. Aust Vet J 1995;72:249‐252.
  8. Sweeney CR, Rossier Y, Ziemer EL, Lindborg S. Effects of lung site and fluid volume on results of bronchoalveolar lavage fluid analysis in horses.. Am J Vet Res 1992;53:1376‐1379.
  9. Jean D, Vrins A, Beauchamp G, Lavoie JP. Evaluation of variations in bronchoalveolar lavage fluid in horses with recurrent airway obstruction.. Am J Vet Res 2011;72:838‐842.
  10. Depecker M, Richard EA, Pitel PH, Fortier G, Leleu C, Couroucé‐Malblanc A. Bronchoalveolar lavage fluid in standardbred racehorses: influence of unilateral/bilateral profiles and cut‐off values on lower airway disease diagnosis.. Vet J 2014;199:150‐156.
  11. Hermange T, Le Corre S, Bizon C. Bronchoalveolar lavage fluid from both lungs in horses: diagnostic reliability of cytology from pooled samples.. Vet J 2019;244:28‐33.
  12. Couetil LL, Cardwell JM, Gerber V. Inflammatory airway disease of horses—revised consensus statement.. J Vet Intern Med 2016;30:503‐515.
  13. Woodrow JS, Hines M, Sommardahl C. Initial investigation of molecular phenotypes of airway mast cells and cytokine profiles in equine asthma.. Front Vet Sci 2022;9:997139.
  14. Kruger P, Saffarzadeh M, Weber AN. Neutrophils: between host defence, immune modulation, and tissue injury.. PLoS Pathog 2015;11:e1004651.
  15. Hartl D, Tirouvanziam R, Laval J. Innate immunity of the lung: from basic mechanisms to translational medicine.. J Innate Immun 2018;10:487‐501.
  16. Steinberg KP, Milberg JA, Martin TR, Maunder RJ, Cockrill BA, Hudson LD. Evolution of bronchoalveolar cell populations in the adult respiratory distress syndrome.. Am J Respir Crit Care Med 1994;150:113‐122.
  17. Balamayooran G, Batra S, Fessler MB, Happel KI, Jeyaseelan S. Mechanisms of neutrophil accumulation in the lungs against bacteria.. Am J Respir Cell Mol Biol 2010;43:5‐16.
  18. Moore WC, Hastie AT, Li X. Sputum neutrophil counts are associated with more severe asthma phenotypes using cluster analysis.. J Allergy Clin Immunol 2014;133:1557‐1563.e5.
  19. Lezmi G, Galmiche‐Rolland L, Rioux S. Mast cells are associated with exacerbations and eosinophilia in children with severe asthma.. Eur Respir J 2016;48:1320‐1328.
  20. Bedenice D, Mazan MR, Hoffman AM. Association between cough and cytology of bronchoalveolar lavage fluid and pulmonary function in horses diagnosed with inflammatory airway disease.. J Vet Intern Med 2008;22:1022‐1028.
  21. Ivester KM, Couëtil LL, Moore GE. An observational study of environmental exposures, airway cytology, and performance in racing thoroughbreds.. J Vet Intern Med 2018;32:1754‐1762.
  22. Cullimore AM, Secombe CJ, Lester GD, Robertson ID. Bronchoalveolar lavage fluid cytology and airway hyper‐reactivity in clinically normal horses.. Aust Vet J 2018;96:291‐296.
  23. Joshi N, Walter JM, Misharin AV. Alveolar macrophages.. Cell Immunol 2018;330:86‐90.
  24. Byrne AJ, Mathie SA, Gregory LG, Lloyd CM. Pulmonary macrophages: key players in the innate defence of the airways.. Thorax 2015;70:1189‐1196.
  25. Garbi N, Lambrecht BN. Location, function, and ontogeny of pulmonary macrophages during the steady state.. Pflugers Arch 2017;469:561‐572.
  26. Herold S, Mayer K, Lohmeyer J. Acute lung injury: how macrophages orchestrate resolution of inflammation and tissue repair.. Front Immunol 2011;2:65.
  27. Hussell T, Bell TJ. Alveolar macrophages: plasticity in a tissue‐specific context.. Nat Rev Immunol 2014;14:81‐93.
  28. Mitsi E, Kamng'ona R, Rylance J. Human alveolar macrophages predominately express combined classical M1 and M2 surface markers in steady state.. Respir Res 2018;19:66.
  29. Gerber V, Robinson NE, Luethi S. Airway inflammation and mucus in two age groups of asymptomatic well‐performing sport horses.. Equine Vet J 2003;35:491‐495.
  30. Michelotto PV Jr, Muehlmann LA, Zanatta AL, Bieberbach EWR, Fernandes LC, Nishiyama A. Platelet‐activating factor and evidence of oxidative stress in the bronchoalveolar fluid of thoroughbred colts during race training.. J Vet Intern Med 2010;24:414‐419.
  31. Michelotto PV Jr, Muehlmann LA, Zanatta AL. Pulmonary inflammation due to exercise‐induced pulmonary haemorrhage in thoroughbred colts during race training.. Vet J 2011;190:e3‐e6.
  32. Moldoveanu B, Otmishi P, Jani P. Inflammatory mechanisms in the lung.. J Inflamm Res 2009;2:1‐11.
  33. Borthwick LA. The IL‐1 cytokine family and its role in inflammation and fibrosis in the lung.. Semin Immunopathol 2016;38:517‐534.
  34. Middleton J, Neil S, Wintle J. Transcytosis and surface presentation of IL‐8 by venular endothelial cells.. Cell 1997;91:385‐395.
  35. Cambier S, Gouwy M, Proost P. The chemokines CXCL8 and CXCL12: molecular and functional properties, role in disease and efforts towards pharmacological intervention.. Cell Mol Immunol 2023;20:217‐251.
  36. Kang H, Lee GKC, Bienzle D. Equine alveolar macrophages and monocyte‐derived macrophages respond differently to an inflammatory stimulus.. PLoS One 2023;18:e0282738.
  37. Padoan E, Ferraresso S, Pegolo S, Castagnaro M, Barnini C, Bargelloni L. Real time RT‐PCR analysis of inflammatory mediator expression in recurrent airway obstruction‐affected horses.. Vet Immunol Immunopathol 2013;156:190‐199.
  38. Curto E, Messenger KM, Salmon JH, Gilger BC. Cytokine and chemokine profiles of aqueous humor and serum in horses with uveitis measured using multiplex bead immunoassay analysis.. Vet Immunol Immunopathol 2016;182:43‐51.
  39. Martin EM, Messenger KM, Sheats MK, Jones SL. Misoprostol inhibits lipopolysaccharide‐induced pro‐inflammatory cytokine production by equine leukocytes.. Front Vet Sci 2017;4:160.
  40. Pavulraj S, Kamel M, Stephanowitz H. Equine herpesvirus type 1 modulates cytokine and chemokine profiles of mononuclear cells for efficient dissemination to target organs.. Viruses 2020;12:999.
  41. Giguere S, Viel L, Lee E. Cytokine induction in pulmonary airways of horses with heaves and effect of therapy with inhaled fluticasone propionate.. Vet Immunol Immunopathol 2002;85:147‐158.
  42. Bond SL, Hundt J, Leguillette R. Effect of injected dexamethasone on relative cytokine mRNA expression in bronchoalveolar lavage fluid in horses with mild asthma.. BMC Vet Res 2019;15:397.
  43. Bond S, Léguillette R. A CONSORT‐guided, randomized controlled clinical trial of nebulized administration of dexamethasone and saline on lower airway cytokine mRNA expression in horses with moderate asthma.. J Vet Intern Med 2024;38:1214‐1223.
  44. Lam S, LeRiche JC, Kijek K. Effect of filtration and concentration on the composition of bronchoalveolar lavage fluid.. Chest 1985;87:740‐742.
  45. Afford SC, Stockley RA, Kramps JA, Dijkman JH, Burnett D. Concentration of bronchoalveolar lavage fluid by ultrafiltration: evidence of differential protein loss and functional inactivation of proteinase inhibitors.. Anal Biochem 1985;151:125‐130.

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