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Topic:Animal Studies
Animal studies involving horses encompass a range of research focused on understanding equine biology, behavior, and health. These studies often investigate various aspects of horse physiology, genetics, nutrition, and disease pathology. Researchers utilize animal studies to explore the effects of different treatments, management practices, and environmental factors on horse welfare and performance. The findings from such studies contribute to the development of improved care strategies and health interventions. This page gathers peer-reviewed research studies and scholarly articles that explore the methodologies, findings, and implications of animal studies conducted on horses, providing insights into their application in advancing equine science.
A perspective on forage production in Canada. Over the past decade, the cattle industry has experienced practically a full circle. With the promising beef prices in the early 1970s, with the glut of grain and a generous assist from government incentive programs, the forage acreage and cattle population have increased at a record rate. By 1974, the tide began to turn - grain prices went up sharply and beef prices became sluggish - and by 1976 a major crisis faced the producers. The cattle industry which had been developing on a cheap grain economy was now obliged to rely more on forage for its survival. Unfortunately, the forage was not ex...
Effect of dehydrocholic, chenodeoxycholic, and taurocholic acids on the excretion of bilirubin. The effects of IV bile acid infusion (at approx 20% of normal excretion rate) on the biliary excretion of 3-alpha-hydroxy bile acids and bilirubin were investigated in ponies prepared surgically with chronic external biliary fistulas. Endogenous bile acid excretion (approx 45 mumol/min) decreased to the hepatic synthesis rate (approx 1.5 mumol/min) during the initial 4 to 5 hours of bile drainage. In type 1 studies, both chenodeoxycholic and taurocholic acid infusion (8 to 9 mumol/min) increased bilirubin excretion by 58% to 82% following 5 hours of biliary diversion. During type 2 studies, 3-...
Mucosal alterations in experimentally induced small intestinal strangulation obstruction in ponies. Small intestinal strangulation obstruction (ISO) was produced in seven ponies (under pentobarbital-anesthesia) by arteriovenous ligation. Positive-pressure ventilation with room air was used to maintain arterial PCO2 at the initiation of the ISO. Biopsy materials obtained from affected intestines at various times were evaluated, using histopathologic examination and scanning electron microscopy. Mucosal and villus degeneration was graded 0 to V and compared with intestinal gross color, motility, and wall thickness. The mucosa at the tip of the villus was the first to be affected. Degeneration ...
Further studies on gangliosides of erythrocytes from horses and cattle. The ganglioside patterns of erythrocytes from individual horses and cattle were examined. Variations in the ganglioside patterns were found in both horses and cattle. In the erythrocytes of most horses examined, NeuGc-Gal-Glc-ceramide (NeuGc-GM3) of 25 horses examined had only NeuGc-GM3 with no 4-O-Ac-NeuGc-GM3. The erythrocytes of various breeds of cattle had a characteristic ganglioside pattern, but they could be divided into 4 types on the basis of the composition of their gangliosides.
Energy and current requirements for ventricular defibrillation using trapezoidal waves. The threshold energy and current required for ventricular defibrillation was determined in dogs ranging in weight from 6.4 to 38 kg and in ponies ranging in weight from 40 to 101 kg. Trapezoidal waves, 10 ms in duration, with 10%, 50%, 70%, and 90% tilt were applied to transchest electrodes. For all values of tilt, the energy and current required increased with body weight. The energy dose (joules per kilogram of body weight) was higher for the heavier animals, whereas the current dose (peak amperes per kilogram of body weight) was essentially the same for dogs and ponies. In both species and ...
Copper-induced GSH depletion and methaemoglobin formation in vitro in erythrocytes of some domestic animals and man. A comparative study. Reduced glutathione (GSH), per cent methaemoglobin and haemolysis were measured in physiologic saline suspensions of erythrocytes from sheep, goat, cattle, horse, swine and man; incubated for 24 hours at 37 degrees with and without copper sulphate (0,5 and 10 micrograms Cu/ml suspension). Formation of Heinz bodies was studied in similar experiments with sheep and swine cells only. Generally, GSH depletion developed first, followed by methaemoglobinemia and Heinz bodies, while haemolysis was limited. Both spontaneous and copper-induced GSH depletion was slow in swine erythrocytes compared to ot...
Comparative studies of alpha-lactalbumin and lysozyme: the proteins of kangaroo (Megaleia rufa and Macropus giganteus) and horse (Equus caballus). As part of a study of the 'whey' proteins of various mammals, a comparison is made of the alpha-lactalbumins and lysozymes of the kangaroo and horse. In the milk of the red kangaroo (Megaleia rufa) there is only one alpha-lactalbumin and it occurs throughout lactation, but no lysozyme has been detected. There are two alpha-lactalbumins in the milk of the grey kangaroo (Macropus giganteus), one, designated alpha-lactalbumin Zone B, is present throughout lactation; the second, designated alpha-lactalbumin Zone A, is present only in late lactation. One lysozyme is also present. The milk of the ho...
Variation of acidic prealbumins in the donkey (Equus asinus). Starch gel electrophoresis of 55 donkey serum samples revealed three prealbumin (Pr) phenotypes temporarily designated Pr M, Pr MT and Pr T. The distribution was in agreement with a genetic theory of two codominant alleles of frequencies, PrM = 0.87 and PrT - 0.13. Variation was also observed for proteins migrating with the same rate as the Xh zones in the horse.
Morphometric and electrophysiologic study of the photoreceptors in the horse. No abstract available
[Structure and topography of nucleus dorsalis in the spinal cord of horses]. The material for the study was taken from 2 spinal cords of sexually mature horses. Preparations obtained from this material were stained according to Nissl and with the use of cresyl violet. The nucleus dorsalis of the horse extends from the 8th cervical neuromere to the 3rd lumbar neuromere of the spinal cord. The cells which form this nucleus lie in the grey matter of the spinal cord, dorsolaterally of the central canal. The nucleus dorsalis is made out of large and medium-size round and oval cells. The characteristic feature of the structure and configuration of this nucleus in the horse i...
A new method for the isolation of aminoacylase from mammalian kidneys. Aminoacylase (E.C. 3.5.1.14) was isolated from the kidneys of different mammalian species (horse, cattle, rabbit and pig) by extracting the organ with water and subjecting the extract to heat treatment at 70 degrees C for 10 min, then, after having removed denatured proteins by fractionating those remaining in the solution by ammonium sulfate. The enzyme obtained in this way can either be used directly for practical purposes (e.g. preparation of immobilized aminoacylase) or further purified by chromatography. For the further purification of porcine kidney aminoacylase we applied a combination ...
An examination of chromosomes in the stallion (Equus caballus) during meiosis. Meiotic preparations were made from testicular material obtained after surgical castration of eight stallions (Equus caballus) with normal spermatogenesis. The material was examined after conventional Giemsa staining and C-banding. C-banding demonstrated that the Y chromosome at diakinesis associated with the short arm of the X chromosome. In 315 cells at diplotene or diakinesis, 56 (17.7%) had univalents and 51 (16.1%) of these involved the sex chromosomes. The overall mean chiasma number was 54.4 +/- 1.8 SD, and the mean calculated nondisjunction (ND) frequency was 3.4%. These results are di...
Equine marker genes. Polymorphism for transferrin alleles, TfF1 and TfF2, in Thoroughbreds. The equine transferrin F variant is distinguishable into two types, F1 and F2, on alkaline polyacrylamide gel electrophoresis. Gene frequencies in 63 related Thoroughbreds are 0.39 and 0.19 for TfF1 and TfF2, respectively. In contrast the frequencies for these two alleles in 375 related Standardbreds is 0.00 and 0.59.
[Pharmacokinetic model studies of sulfamerazine in domestic mammals. 1. Elimination of Mebacid 200 following intravenous administration to large animals]. Pharmacokinetic data of sulphamerazine were recorded from eight heads each of calf, adult cattle, horse, and sheep, following intravenous application of Mebacid 200, and mathematical implications were discussed. Exponential excretion was recorded from all species, according to the following equation: c = B x e-k2 x t The most favourable pharmacokinetic parameters were recorded from calf.
The origin of nuclear bodies: a study of the undifferentiated epithelial cells of the equine small intestine. During an electron and light microscopic study of the equine intestinal epithelium, it was observed that some secretory granules of the undifferentiated crypt epithelium were incorporated into the nucleus during mitosis. A study was made of the chemical nature of the granules, using standard histochemical techniques: PAS-Alcian blue, Deamination-PAS, and Ninhydrin-Schiff reactions. The granules contained a neutral protein-polysaccharide complex with many terminal amino groups, possibly an antibody (IgA). The intranuclear granules underwent coalescence and degeneration during differentiation. T...
Quantitative comparisons of acidic prealbumin (PR) phenotypes in horses. Comparisons of Pr protein amounts in horse sera have been performed using .’s (1965) immunodiffusion technique. Relative values against a chosen standard of 100 % were determined for a total of 435 horses. There was considerable variation between horses, the highest Pr value being 125 and the lowest 50 % of the standard. In animals of the same Pr phenotype the mean Pr values were significantly higher (P < 0.001) in foals than in mares. In Norwegian Trotter horses the Pr value of Pr NN animals was significantly higher than that of Pr SS phenotypes, whereas the mean Pr values of Pr SS was sig...
