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Topic:Biochemistry
The study of biochemistry in horses encompasses the chemical processes and substances that occur within equine organisms. This field investigates the molecular interactions and pathways that are fundamental to horse physiology, including metabolism, enzyme activity, and genetic expression. Key areas of interest include the examination of metabolic disorders, nutrient absorption, and the biochemical basis of muscle function and energy production. Researchers utilize biochemical analysis to understand health and disease mechanisms in horses, contributing to the development of diagnostic tools and therapeutic strategies. This page gathers peer-reviewed studies and scholarly articles that explore various biochemical processes and their implications for equine health and performance.
Equine marker genes: Polymorphism for soluble erythrocyte malic enzyme. Polymorphism of equine erythrocyte malic enzyme is detactable on starch gel electrophoresis. The frequency of ME1S was 0.06 in 667 Standardbred and 0.09 in 85 Thoroughbred horses. No genetically determined electrophoretic variation in soluble malate dehydrogenase was detected.
Copper metabolism and requirement in mature ponies. The effects of copper intake on stable copper and 64Cu metabolism and on the excretory pathways of absorbed copper were examined in ponies. Bile and feces collected from two bile duct-cannulated ponies following an intravenous dose of 64Cu showed that bile was the main route of endogenous copper excretion. Stable copper and 64Cu metabolism were examined in three intact ponies fed dietary copper to provide 5.6, 16.6 and 25.7 mg Cu/100 kg body weight/day. The amount of copper excreted in urine was not influenced by dietary treatment. Stable copper absorption and 64Cu retention significantly incr...
Fatty acid composition of equine plasma. Fatty acid composition of plasma lipids of normal horses was determined. Four fatty acids (C16:0, C18:0, C18:1, and C18:2) comprised 86.73% of the total, with C18:2 comprising 44.04% of the total. Eight other fatty acids were found in small amounts. Unsaturated fatty acids constituted 66% of the total. Marked variation was demonstrated in fatty acid occurrence and distribution in the sterol ester, triglyceride, phospholipid, and free fatty acid fractions.
Interaction of horse plasma antithrombin III and alpha 1-proteinase inhibitor with some serine proteinases. Antithrombin III and alpha 1-proteinase inhibitor isolated simultaneously from horse citrated plasma were tested for inhibitory activity against bovine trypsin and chymotrypsin, as well as elastase-like neutral proteinases from horse leucocytes. The stoichiometry of reaction and kinetic parameters (kass, Ko) were estimated and related to the protein pattern obtained after exposure of these proteinases to horse inhibitors as analyzed by polyacrylamide gel electrophoresis (PAGE and PAGE-SDS). As shown by fast reaction rates and low values of dissociation constants the two inhibitors effectively ...
[Variations in plasma components in lactating mares at late pregnancy-early lactation (author’s transl)]. In an experiment comparing pregnant then lactating mares with dry non-pregnant mares, the changes in plasma components were studied as indicators of the metabolic utilization of energy (glucose, non-esterified fatty acids, beta-hydroxybutyrate), nitrogen (urea, proteins) or minerals (calcium, phosphorus, magnesium). The mares were fed daily 2 kg concentrate and hay ad libitum. Beta-hydroxybutyrate, magnesium and to a lesser extent glucose were the only constituents whose levels were affected within the period starting one month before and ending one month after foaling. The increase in food in...
Variations in the properties of equine chorionic gonadotropin. The objectives of this paper are to review the chemical and biological properties of equine chorionic gonadotropin (eCG, PMSG) isolated from the serum. Comparisons are made with eCG isolated from endometrial cups, trophoblast cell culture medium, and low titer serum. The results show that eCG can vary, depending on the source, in both chemical and biological (LH and FSH activity) properties.
Antigen-antibody crossed electrophoretic studies and quantitative comparisons of serum transferrin types in horses. Selected transferrin phenotypes from 14 horses were investigated by antigen-antibody crossed electrophoresis. Horse sera were subjected to starch gel electrophoresis followed by right angle electrophoresis in agarose gels containing rabbit produced anti-horse transferrin. This technique gave an additional zone in the front as compared with 2 transferrin zones seen after ordinary starch gel electrophoresis. Comparisons of transferrin concentrations in horse sera were performed by an immunodiffusion technique. Values were related to a chosen reference serum. A total of 372 horses (210 Norwegian ...
Influence of dietary molybdenum on copper metabolism in ponies. The effects of molybdenum ingestion on copper metabolism were investigated in bile duct-cannulated ponies fed diets containing 1.01, 27.4 and 107.3 ppm molybdenum. Copper metabolism was assessed by examination of 64Cu and stable copper excretion in feces, bile and urine. The addition of molybdenum to the diet decreased copper absorption and retention as a consequence of increased excretion of dietary copper in feces and increased excretion of absorbed copper in bile. Only 5% or less of the absorbed copper was excreted in urine regardless of dietary treatment. Biliary molybdenum excretion was o...
In vivo metabolism of [3H]equilin in the pregnant mare. [3H]Equilin [3H-labeled 3-hydroxy-1,3,5(10), 7-estratetraen-17-one] was administered iv to a pregnant mare in the 10th month of gestation. Maternal urine was collected for 3 days, and blood samples were taken 35 min and 3, 6, 12, and 24 h after the injection. The half-life of the disappearance of radioactivity from the blood was approximately 2.5 h. Over 90% of the administered dose was excreted in the first 24 h. The urine was extracted, hydrolyzed, and fractionated. The bulk of the radioactive material (75%) was present in the phenolic sulfate fraction from which radiochemically pure equilin...
Characterization of the binding of Triton X-100 to equine and rabbit serum albumin. The binding isotherms for Triton X-100 binding to equine and rabbit serum albumin were determined by equilibrium dialysis at 16 degrees C in pH 7.0, I = 0.05 phosphate buffer. Presented in a Scatchard plot, the binding isotherms are a straight line, indicating thermodynamically independent and identical binding sites. In this model equine serum albumin is characterized as having 11 such sites with an equilibrium constant of 6.0 x 10(3) M-1. Similarly, rabbit serum albumin is characterized as having 9 such sites with an equilibrium constant of 8.0 x 10(3) M-1.
The metabolism of promazine and acetylpromazine in the horse. Promazine hydrochloride and acetylpromazine maleate were administered intravenously at clinical dose levels to horses. In urine from horses given promazine hydrochloride, the parent drug and four metabolites were detected. The two major metabolites, present as conjugates were identified after hydrolysis by beta-glucuronidase/arylsulfatase as 3-hydroxypromazine and 3-hydroxydesmonomethyl-promazine. Conjugated 3-hydroxypromazine has been previously identified as a major metabolite in the horse. Two minor metabolites isolated in this study were primaizine N-oxide and promazine N-oxide sulfoxide. ...
[Some clinical biochemical features of ‘tying up’ in horses (author’s transl)]. The changes in the activities of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) in de blood of thirty-three horses with 'tying up' were compared. The extent to which the serum enzymes LDH, CPK and glutamic oxaloacetic transaminase (GOT) and the changes in the activities of these enzymes after suitable labour can be used in the diagnosis of 'tying up' and in following the recovery of patients was studied.
A biochemical study of the Arab Horse Society’s marathon race. Blood samples were taken before and after a cross country race over the marathon distance of 42 km. There was a rise in blood glucose and plasma free fatty acids and glycerol associated with a rise in plasma cortisol and glucagon but the fall in insulin was not significant (P > 0.05). Plasma potassium and albumin concentrations increased, calcium decreased and there was no change in sodium or bicarbonate concentrations. There was an increase in plasma urea, creatinine, uric acid, bilirubin and isocitrate dehydrogenase but no change in alkaline phosphatase. There was a rise in plasma creatine k...
Fractionation and partial characterization of alpha-1-protease isoinhibitors of horse. The principal alpha-1-protease inhibitor of horse was fractionated by classical methods and analysed with a modified fibrinogen-agarose gel electrophoretic method of high sensitivity and resolving power. Starting with an electrophoretically homogeneous inhibitor in unfractionated serum, two isoinhibitor bands became apparent after fractionation with (NH4)2SO4 and DEAE-cellulose DE-52 ion-exchange chromatography. The isoinhibitors differed in electrophoretic migration and in the elution pattern from Sephadex G-100 gel filtration, but possessed identical antigenic determinants and enzyme specifi...
Effects of copper pretreatment upon toxicity of selenium in ponies. Thirty-four adult ponies were used to determine the effects of single oral doses of copper (Cu) supplements (0, 20, and 40 mg of Cu/kg of body weight) on the toxicity of oral doses of selenium (Se) supplements (0, 2, 4, 6, and 8 mg of Se/kg of body weight) administered 24 hours after the copper was given. Signs of Se toxicosis-sweating, diarrhea, tachycardia, tachypnea, mild pyrexia, lethargy, and colic-developed in ponies given 6 and 8 mg of Se/kg of body weight without Cu pretreatment. Two of 4 ponies given 6 mg of Se/kg and both ponies given 8 mg of Se/kg without Cu pretreatment died within...
Characterization of equine alkaline phosphatase isoenzymes based on their electrophoretic mobility by polyacrylamide gel disc electrophoresis. Alkaline phosphatase isoenzymes of equine tissues, peritoneal fluid, and serum were characterized by their electrophoretic mobilities, using polyacrylamide gel disc electrophoresis. The alkaline phosphatase isoenzymes in liver, kidney, spleen, small intestine, placenta, bone, small colon, and large colon tissue samples were extracted and separated by electrophoresis. The resulting isoenzyme mobilities and spectrophotometric scans were evaluated for their tissue specificity and for their possible use in determining the tissue contribution of alkaline phosphatase to serum and peritoneal fluid. T...
Dietary fat and exercise conditioning effect on metabolic parameters in the horse. Four isocaloric diets containing 4, 8, 12 and 16% dietary fat (as soybean oil) were fed to four horses at four intervals according to a Latin square design. After 3 weeks of conditioning at each interval, diet effects were evaluated by trotting all horses at 3.2 m/sec for 6 hours. Pre- and posttrotting responses were measured in muscle and liver glycogen, serum long-chain fatty acids, serum electrolytes, serum enzymes, serum cholesterol, plasma glucose, packed cell volume and hemoglobin. Dietary fat was highly correlated with exercise-induced plasma glucose changes and with cholesterol concent...
Equine postanesthetic forelimb lameness: intracompartmental muscle pressure changes and biochemical patterns. Intracompartmental muscle pressures were recorded from the right and left forelimbs (extensor carpi radialis, triceps brachii) of healthy horses maintained in left lateral recumbency while under deep halothane anesthesia for 180 to 240 minutes. Cardiac output, blood pressure, blood gases, and acid-base status were monitored throughout the anesthesia, and electrolyte levels (Ca2+, P+, K+, Cl-, Na+) and enzyme activities (aspartate aminotransferase (AST), creatine phosphokinase (CPK), and blood lactate) were monitored for 7 days. Postanesthetic forelimb lameness was produced in 5 of the 6 horses...
[Effect of 2 methods of demineralization on the on the preservation of glycoproteins and proteoglycans in the intertubular and peritubular dentin in the horse]. The effect of 2 methods of demineralization on the preservation of proteoglycans and glycoproteins was studied in the intertubular and peritubular dentine of the horse. The specimens embedded in Epon were demineralized with a 2% acid formic solution (Bonucci and Gheradi, 1975). Other fragments were treated with an organic solution of EDTA alkylammonium salt (Scott and Kyffin, 1979). These methods preserved in a satisfactory way these labile organic components. In the intertubular dentine, glycoproteins and proteoglycans were also identified, either associated with collagen fibres as a glue and...
Helix packing and subunit conformation in horse spleen apoferritin. An electron density map of horse spleen apoferritin at 0.28-nm (2.8 A) resolution and its preliminary interpretation have been described previously. Rigorous examination of this and newer maps at the same nominal resolution but calculated from more extensive data sets, including model building in a Richards' comparator, now allows us to report on structural features in more detail. We list inter-helical angles within and between neighbouring subunits, and describe a new short region of inter-subunit anti-parallel pleated sheet. A short section of electron density not properly accounted for in ...
[The immunological relation between human and equine Gc proteins (author’s transl)]. The immunological comparison of human and equine Gc proteins showed partial identical reactions between both species. Immunizations of goats and rabbits with horse serum produced antisera able to recognize human Gc proteins.
Comparison of milk and blood plasma progesterone concentrations in cycling and pregnant mares. Progesterone concentrations were measured in milk and blood plasma for 15 mares throughout a normal estrous cycle and early pregnancy to determine the feasibility of utilizing progesterone in milk as an indicator of pregnancy. Samples were obtained daily from foaling until diagnosis of pregnancy by rectal examination at 30 to 35 days of gestation. Progesterone in milk and blood plasma was quantified by radioimmunoassay. Mean progesterone concentrations (+/- SE) in plasma from foaling to foal heat and during estrus, luteal phase and pregnancy were .51 +/- .09 ng/ml, .53 +/- .08 ng/ml, 3.88 +/- ...
The major metabolite of fentanyl in the horse. Fentanyl, a potent, synthetic narcotic analgesic, has reportedly been used to "dope" racehorses. Urine was collected from a horse dosed with 70 mg of [3H]fentanyl, and the primary metabolite, a water-soluble, amphoteric compound, was isolated by high-pressure liquid chromatography and identified by spectroscopic analysis. This metabolite was found to be N-[1-(2-phenethyl-4-piperidinyl)] malonanilinic acid.
