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Topic:Comparative Study
Comparative studies in equine research involve the systematic analysis of different horse breeds, management practices, or physiological responses to identify variations and similarities. These studies are instrumental in understanding how different factors influence health, performance, and behavior in horses. Common areas of comparison include genetic traits, nutritional requirements, disease resistance, and response to training. By evaluating these differences, researchers can develop targeted strategies for breeding, healthcare, and training. This page aggregates peer-reviewed research studies and scholarly articles that focus on the methodologies, findings, and implications of comparative studies in the context of equine science.
A comparative review of human and equine leucocyte differentiation antigens. Monoclonal antibody technology has allowed the recognition and study of numerous leucocyte antigens in man and laboratory animals for over a decade. Numerous advances in the understanding of immune responses and immunopathology have resulted. In recent years equine researchers have started to develop similar reagents, which now offer a powerful tool to investigators of equine immunology and disease.
A comparison of ELISA, FAST-ELISA and gel diffusion tests for detecting antibody to equine infectious anaemia virus. Sera of sixteen horses with clinical signs of EIA from six different outbreaks and sera of 100 uninfected horses were used to validate an ELISA for EIA diagnosis. The antigen used was a recombinant protein derived from the amino-terminal portion of the transmembrane envelope protein of EIA (gp45). Reactivity between positive and negative sera could be clearly distinguished. Comparison with the traditional agar gel immunodiffusion test (commonly called the Coggins test) showed that the ELISA was superior in sensitivity. Comparison of this ELISA with the FAST-ELISA system showed that the latter ...
The estimation of factor VIII levels in horse, cattle, sheep and pig plasma by the use of synthetic chromogenic substrate. Factor VIII level in horse, cattle, sheep and pig plasma was estimated by the use of synthetic chromogenic substrate S-2222 (benzoyl-isoleucyl-glutamyl-glycyl-arginyl-p-nitronilide). The highest level of this factor was stated in pig, the lowest one in sheep plasma.
[DNA fingerprinting in horses]. Using a multilocus DNA probe, individual - specific hybridization patterns, the so-called DNA fingerprints (TAB) were determined in six horse families by the DNA fingerprinting method. The probe with evolutionally preserved nucleotide sequence from bacteriophage M13 determines hypervariable regions placed in genomic minisatellite DNA. The use of this probe permits an identification of an individual and execution of paternity relationships with a probability over 99.99 per cent.
Plasma potassium measurement with a new reagent carrier (Reflotron): comparison with ion-selective electrode results. Potassium concentrations were measured in the plasma of 336 animals with a new reagent carrier (Reflotron; Boehringer Mannheim) K+ and with an ion-specific electrode system: results were highly correlated (r = 0.991; y = 0.993 x + 0.02) and day-to-day coefficient of variation of the new reagent measurements was lower than 2.5 per cent. This system offers a good alternative to the ion-selective electrode system for plasma potassium measurement in veterinary practice.
Electropherotypes, serotypes, and subgroups of equine rotaviruses isolated in Japan. Electropherotypes (ET), serotypes, and subgroups of equine rotaviruses isolated from foals in Japan were determined. The ETs of 136 isolates from 1981 through to 1991 were divided into six groups: ET-A-ET-F. The ET-A, -B, -C, -D, -E, and -F were present in 3, 1, 121, 9, 1, and 1 strains, respectively. Representative viruses of ET-A, -B, -C, and -D were identified as serotype G3. Viruses of ET-E and -F were identified as serotypes G 10 and G 5, respectively. The four representative viruses of serotype G 3 did not belong to either subgroup I or II. The two viruses of serotypes G 5 and G 10 belon...
Coronary arterial anatomy of the one-humped camel (Camelus dromedarius). The detailed coronary arterial anatomy of seven camels was studied and compared with that of horses and cattle. In camels, there is a bilateral coronary supply, the right coronary artery being the larger. The left coronary artery follows the same pattern as that in horses. The ramus collateralis proximalis in camels separates off some distance away from the origin of the ramus interventricularis paraconalis, as in horses, whereas it separates of very quickly in cattle. The ramus collateralis distalis has two branches in camels, whereas the left distal ventriculi ramus does not branch. The ramu...
[Hemolytic properties of bacteria belonging to the Acinetobacter genus]. Direct and intermediate hemolytic activity of 526 strains of Acinetobacter was investigated. Their ability to produce lipase and lecithinase was also studied. Measurements were performed parallely on human, horse, sheep and bovine erythrocytes. Direct hemolytic activity was exhibited by 16% of tested strains (17 out of 24 strains of A. haemolyticus). Human, sheep and bovine erythrocytes were useful for testing the hemolytic activity of Acinetobacter. The hemolysis was occurring faster and was visible more frequently during incubation at 37 degrees C. Indirect hemolytic activity was observed in...
Assay for endogenous heparin in plasma of livestock using a synthetic chromogenic substrate. The levels of endogenous heparin in the plasmas of horses, cows, sheep and pigs were determined with the use of synthetic chromogenic substrate benzoyl-isoleucyl-glutamyl-glycyl-arginyl-p-nitroanilide (S-2222). The lowest heparin concentrations were stated in cattle plasma, the highest ones in the plasma of pigs.
Recommended terminology for researchers in locomotion and biomechanics of quadrupedal animals. This paper summarizes recommendations for terminology to be used in the description of quadrupedal locomotion and selected aspects of biomechanics. Directional terms and planes of the body (anatomical position, spatial reference systems), joint angulation, conformation, general locomotion terminology, phases of the stride and limb cycle (e.g. step, cadence) and terminology for the description of jumping are described.
Stability of sorbitol dehydrogenase activity in bovine and equine sera. Serum sorbitol dehydrogenase (SDH) activities in 10 cows and nine horses were measured using an automated clinical analyzer. The serum samples were divided into aliquots that were stored at room temperature (21 degrees C), refrigerated (0-5 degrees C), or frozen (-30 degrees C). The stability of the SDH activity was monitored at various intervals. SDH activity in bovine sera remained stable for at least 5 hours at room temperature, 24 hours refrigerated, and 72 hours frozen without any significant (p < 0.05) differences from the initial serum values. In equine sera, SDH activity remained st...
DNA of bovine papillomavirus type 1 and 2 in equine sarcoids: PCR detection and direct sequencing. Nucleotide sequences of bovine papillomavirus (BPV) DNA amplified by the polymerase chain reaction (PCR) from samples of equine sarcoid skin tumours were determined. All naturally occurring sarcoids (n = 58 tumours from 32 horses and 2 donkeys) contained BPV-DNA. All but 3 of the genome fragments belonged to the BPV type 1 strain (BPV-1); the remaining were BPV type 2. Similar results were obtained with cutaneous bovine papillomas used as controls (n = 20). One of the horses, carrying 2 sarcoids, was particularly interesting; one tumour contained BPV-1 DNA whilst the other sarcoid yielded BPV-...
Caffeine contractures, twitch characteristics and the threshold for Ca(2+)-induced Ca2+ release in skeletal muscle from horses with chronic intermittent rhabdomyolysis. Muscle from horses with intermittent exercise associated rhabdomyolysis was examined to determine if calcium regulation was abnormal. In vitro studies on semimembranosus muscle fibre bundles showed the time to 50 per cent relaxation of caffeine-induced contractures was shorter and the electrically elicited twitch longer in horses with exercise associated rhabdomyolysis. Substitution of strontium for calcium eliminated the difference in caffeine contracture between the normal and rhabdomyolysis horses. The threshold of calcium-induced calcium release was lower than normal in terminal cisternae-...
The extended canter: a comparison of some kinematic variables in horses trained for dressage and for racing. This study was designed to test the hypothesis that there is no significant difference in selected temporal and linear stride variables of the extended canter in horses bred and trained for dressage or racing. Nine advanced-level dressage horses and 7 Thoroughbred racehorses were filmed at a frame rate of 200 Hz at an extended canter on a sand track. Two strides were recorded per trial, and each horse performed 6 or 7 trials. Temporal and linear data were determined from the films, and descriptive statistics (mean, SD) were calculated. Strides were selected for analysis on the basis of having ...
[The use of ELISA and indirect immunofluorescence technics for the rapid detection of eastern equine encephalomyelitis]. We present the results attained in the identification of Eastern equine encephalomyelitis virus isolations in Vero and XL-2 cell systems, using a double-antibody ELISA technique and the indirect immunofluorescence method. The results attained through these two techniques coincided by 100% with identification through neutralization. With the former, the virus was detected within 6-8 hours after inoculation. Better results were attained with XL-2 cells.
Haematological parameters of the Polish Primitive Horses. Average values of chosen haematological parameters have been described in a population of 90 clinically normal Polish Primitive Horses. The comparison of the results with values given in literature for horses as a whole and for other breeds enabled us to state that Polish Primitive Horses' blood is characterized by relatively low values of RBC, PCV, segmented neutrophils and monocytes, and high percentage of lymphocytes. It was demonstrated that values of some blood parameters of examined animals are similar to typical results obtained for primitive and cold-blooded horses. Moreover, it was pr...
Limits to maximal performance. Body size fundamentally affects maximal locomotor performance in mammals. Comparisons of performances of different-sized animals yield different results if made using relative, rather than absolute scales. Absolute speed may be a reasonable way to evaluate the locomotor performance of an animal that must escape predators in real time. However, comparisons of metabolic power in animals of different size can only be made meaningfully on a mass-specific basis. Numerous factors associated with the mechanics, energetics, and storage of elastic energy during locomotion change with body size, which r...
Quantitative determination of equine alkaline phosphatase isoenzymes in foal and adult serum. Automated and semiautomated assays were developed and validated for the determination of equine alkaline phosphatase isoenzymes including intestinal (IALP), bone (BALP), and liver (LALP). The addition of levamisole selectively inhibited more than 97% of LALP while inhibiting only 55% of IALP. Because these percentages were highly reproducible in an automated system, the IALP activity could be calculated in a sample. Bone alkaline phosphatase isoenzyme was selectively precipitated by adding an equal volume of wheat germ agglutinin (5 mg/mL), incubating for 30 minutes at 37 degrees C, and centri...
[Comparison of the career profiles of racehorses over three decades]. To compare the career profiles of thoroughbred racehorses in Germany in different decades all horses which raced for the last time in the years 1966/67, 1976/77 and 1986/87 were selected from the annual report of the German Thoroughbred Racing Association. The number of racing horses increased within decades while the percentage of the horses which raced for the last time was equal in all years. It amounted yearly to about 30%. Most of the horses finished racing at three and four years of age. In 1966, 1967, 1976 and 1977 more than 50% of the horses began their career at the age of two years, ...
Quantification of the locomotion of Dutch Warmblood foals. To get an in-depth insight into the ontogeny of the equine locomotor apparatus, the locomotion of 28 Dutch Warmblood foals was recorded at the walk, trot and canter on a treadmill using a CODA-3 apparatus. A great similarity in joint angle-time diagrams was found, although considerable differences in kinematic gait parameters of individual foals could be detected. These results contribute to an objective comparison of the locomotor performance of foals.
Pathogenic studies and antigenic and sequence comparisons of A/equine/Alaska/1/91 (H3N8) influenza virus. An influenza virus, A/equine/Alaska/1/91 (H3N8), was isolated from horses from Alaska with an acute respiratory infection. Pathogenic and serologic studies revealed that this virus is similar to previously isolated equine H3N8 influenza viruses. Antigenic analyses utilizing hemagglutination inhibition and neuraminidase inhibition assays indicated an antigenic drift from the prototype equine H3N8 influenza virus, A/equine/Miami/1/63. Partial sequence analysis of the A/equine/Alaska influenza virus indicated that each of 8 gene sequences are of equine origin.
Stride characteristics of overground versus treadmill locomotion in the saddle horse. The purpose of this study was to compare the velocity-dependent changes in stride length and stride frequency of horses performing the same incremental exercise test first on a track with a rider and then on a treadmill with a 0 and 3.5% slope successively. Seven French saddle horses undertook the exercise test which consisted of 5 stages of increasing speed for 3 min each with 1 min rest between each stage (1.6, 4.2, 8.3, 9.2 and 10 m/s). The horses were recorded by use of a video camera, and the tapes were analysed with regard to gait parameters. At 1.6, 8.3, 9.2 and 10 m/s, stride frequenci...
Eosinophilic cytoplasmic inclusions in sporadic equine motor neuron disease: an electron microscopic study. Equine motor neuron disease (EMND) is a sporadic, progressive neurodegenerative disorder that has been identified recently in horses of different breeds in North America. The cause is unknown. Pathologic changes which occur in spinal and certain brain stem motor neurons include chromatolysis, swelling, neurofilamentous accumulation, and development of eosinophilic cytoplasmic inclusions. Punctate eosinophilic inclusions, the type usually encountered in degenerating neurons, resembled Bunina bodies at the light microscopic level, but differed in their ultrastructural composition. These and less...
Effect of carnitine supplement to the dam on plasma carnitine concentration in the sucking foal. The changes in carnitine in plasma and milk during the first 3 months of lactation were studied in 14 broodmares and their foals. Six of the mares (Group S) were given a supplement of 10 g carnitine split between the morning and evening feeds, starting 2 weeks before birth. At birth the plasma carnitine concentration in Group S mares was about twice that in Group NS mares (no supplement). In both groups the concentration initially declined in the days after birth. Whilst this trend was reversed in Group S mares, the concentration in Group NS mares remained at a reduced level for the remainder ...
