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Topic:Comparative Study
Comparative studies in equine research involve the systematic analysis of different horse breeds, management practices, or physiological responses to identify variations and similarities. These studies are instrumental in understanding how different factors influence health, performance, and behavior in horses. Common areas of comparison include genetic traits, nutritional requirements, disease resistance, and response to training. By evaluating these differences, researchers can develop targeted strategies for breeding, healthcare, and training. This page aggregates peer-reviewed research studies and scholarly articles that focus on the methodologies, findings, and implications of comparative studies in the context of equine science.
Serum protein electrophoresis in horses and ponies. A method of electrophoresis of horse serum on agarose gels (pH 8.6) is described, together with a system for interpreting changes in the electrophoretic zones based upon the relative distribution of the major serum proteins. Differences in the protein composition of the individual electrophoretic zones of horses and ponies were recorded, although this variation probably reflects differences in management and the presence of subclinical disease.
Connective tissue composition of the equine sarcoid. The connective tissue composition and organisation of the "equine sarcoid" was compared with that of normal adult equine skin to determine whether the cells which produce their respective connective tissue matrices show similar biosynthetic characteristics. No major qualitative difference could be found between the collagen compositions of skin and sarcoid material, although the organisation into fibres of Type III collagen in the sarcoid was markedly greater than that of skin.
Glutathione peroxidase and selenium in the blood of healthy horses and foals affected by muscular dystrophy. When blood selenium concentrations and glutathione peroxidase activity was measured in 30 standardbred horses a significant correlation was found (r = 0.97). A comparison between blood GSH-px activity in clinically healthy foals, foals affected by muscular dystrophy (MD) and their respective mares was also done. There was no difference in GSH-px activity between the healthy foals and the MD foals or between the corresponding mares.
Enzyme activities and protein concentration in the intraocular fluids of ten mammals. An attempt was made to establish normal values for the total protein concentrations and the enzyme activities of LDH, MDH and PGI in the intraocular fluids of rats, guinea pigs, rabbits, cats, dogs, sheep, cattle, pigs, horses and humans. Remarkably little species differences were noted in 9 of the 10 mammals with vitreal enzyme activities falling into a narrow range between 8.4 U/l (PGI, horse) and 92.4 U/l (MDH, guinea pig). All species obeyed the sequence aqueous less than vitreous less than serum with exception of the rat, where vitreous activities surpassed serum at least two-fold. The ve...
A comparison of chemical and electrophoretic methods of serum protein determinations in clinically normal domestic animals of various ages. The biuret total protein method and a bromcresol green (BCG) albumin method were used on the Abbott ABA-100 chemistry analyzer to assay serum proteins in clinically normal cattle, sheep, ponies, pigs, and ducks. Total proteins were also read on a refractometer and mylar supported cellulose acetate electrophoresis was performed. Globulins and A/G ratios were calculated from the chemical method and the results compared with the electrophoretic method. Total protein, albumin and A/G ratios in the ponies, sheep and older cattle were in agreement between the two methods. The younger cattle and all ...
Observations on the isoenzymes of creatine kinase in equine serum and tissues. The isoenzymes of creatine kinase have been measured in serum and selected tissues from horses. The distribution followed that reported in other species in that the MM dimer of the isoenzyme was present in voluntary and non-voluntary muscle, thyroid, liver, spleen, lung and intestine. The BB dimer of the isoenzyme was predominant in brain, pancreas, kidney, intestine, lung, spleen, liver and thyroid. In contrast, in 4 hearts examined less than 1.5 per cent of the total creatine kinase activity was attributable to the MB form of the isoenzyme. The MB isoenzyme was, however, present in intestine...
Observations on the isoenzymes of aspartate aminotransferase in equine tissues and serum. The distribution of the isoenzymes of aspartate aminotransferase (AST, E.C. 2.6.1.1.) in equine tissues has been studied to ascertain whether the organ of origin may be identified when the total AST activity of serum is raised. Most tissues contain 3 isoenzymes of cytoplasmic origin (cAST) with isoelectric points of 5.6, 5.7 and 5.9, and one isoenzyme of mitochondrial (mAST) origin with an isoelectric point of 9. Serum from horses with azoturia contained an additional cytoplasmic subform with an isoelectric point of 5.8. This form could not be generated by ageing, freezing and thawing or bindi...
Liver scintigraphy in ponies. Six derivatives of ethylenediamine-N,N'-bis (alpha-2-hydroxy phenyl) acetic acid labeled with technetium 99m were prepared and their imaging qualities evaluated in ponies. The 6 agents produced good scintigraphic images of certain structures of the liver in the pony. For each agent, 13 different scans were taken. Dorsal views of the left lateral, right lateral, and quadrate lobe were obtained with dorsal scans. Left lateral and left lateral oblique (45 degrees) scans provided a left lateral view of the left lobe and a medial view of the right lateral lobe. Right lateral scans revealed the righ...
Phagocytosis and intracellular killing of the contagious equine metritis organism by equine neutrophils in serum. Equine neutrophils were combined with Haemophilus equigenitalis (contagious equine metritis organism; CEMO) or Escherichia coli in low- and high-antibody-titer serum to evaluate the neutrophils ability to phagocytize and kill these bacteria. More E. coli than CEMO were phagocytized at each time period. After 120 min in low-antibody-titer serum, 56.3% of the E. coli and 34.3% of the CEMO were phagocytized. A total of 45% of CEMO and 74.9% of E. coli were phagocytized by 120 min when neutrophils were in high-antibody-titer serum. More than 75% of the ingested E. coli and 90% of the ingested CEMO...
Enzyme-linked immunosorbent assay for diagnosis of equine infectious anemia. An enzyme-linked immunosorbent assay (ELISA) was elaborated for the detection of specific antibody to equine infectious anemia (EIA) antigen. Sera from horses experimentally infected with EIA virus were assayed by ELISA, complement fixation (CF) and immunodiffusion (ID) tests for antibody to EIA antigen. The ELISA technique was found to be much more sensitive than CF and ID tests. In addition, EIA specific antibody could be detected by ELISA at an earlier stage of infection than by CF or ID techniques. The applicability of the technique to diagnosis of EIA is discussed.
[Some physicochemical properties of native and polymerized glutaraldehyde-treated horse heart cytochrome c]. Glutaraldehyde treatment does not change the absorption of cytochrome c either in the visible or in UV spectra. It brings about the formation of dimers, trimers and high-polymeric forms of cytochrome c and shifts the pI of all cytochrome c isoelectric fractions to more acid pH. Polymerization also results in changes of kinetic parameters of cytochrome c benzidine reaction increasing its affinity to 3,3-diaminobenzidine with a simultaneous decrease in the effectiveness of H2O2 binding. These biochemical changes can be related to immunochemical differences of native and glutaraldehyde-treated cy...
Morphometry of equine neutrophils isolated at different temperatures. Equine neutrophils were evaluated ultrastructurally and by morphometric analysis. Homogeneous populations of neutrophils were isolated from peripheral blood at 4 degrees and 22 degrees C by centrifugation on two sequential Ficoll-Hypaque density gradients. Isolation procedures at both temperatures resulted in neutrophil degranulation but not cell swelling. Degranulation was more extensive in cells isolated at 22 degrees C. Isolation temperature affected the neutrophil content of secondary granules more than primary granules. A granule similar to immature specific granules of human neutrophils ...
Periosteal transection and periosteal stripping for correction of angular limb deformities in foals. Valgus deformities were created in 6 pony foals by hemicircumferential transection of the periosteum and periosteal stripping (HCTP and PS) just proximally to the distal physis on the medial side of 1 radius (principal thoracic limb). The opposite thoracic limb served as a control. One month after this surgical procedure was done, the limbs were radiographed and the angle of deviation was determined. All horses developed a valgus deformity of the principal limb. In an effort to correct the acquired valgus deformity, the 2nd surgical procedure was performed--HCTP and PS on the lateral aspect of...
The measurement of liquid and solid digesta retention in ruminants, equines and rabbits given timothy (Phleum pratense) hay. 1. Digesta passage and retention were measured in heifers, sheep, goats, equines and rabbits of varying body-weights when given timothy (Phleum pratense) hay. 2. Two passage markers were compared, cobalt (III) ethylene diamine tetraacetate (CoEDTA) and chromiummordanted timothy fibre for liquid and solid phase respectively. Both markers were injected into the rumen of the ruminants and into the caecum of the equines and rabbits. 3. In ruminants, two different sets of rate constants (k1 and k2) were derived from a two-pool model for marker passage, using a graphical approach and a computer-base...
Deficiency of interferon-gamma but not interferon-beta in Arabian foals with severe combined immunodeficiency. The results of a study on the induction of IFN-alpha, IFN-beta, and IFN-gamma in normal and SCID foals showed a deficiency of IFN-gamma but not IFN-beta in SCID foals. The ability of SCID mononuclear cells to produce IFN-alpha in response to poly I:C but not to NDV may indicate a partial deficiency of IFN-alpha in SCID foals. The deficiency of IFN-gamma and presence of IFN-beta in SCID foals supports the classification of IFN-gamma and IFN-beta as immune and nonimmune interferons, respectively. Furthermore, the deficiency of IFN-gamma in SCID foals may in part explain the high susceptibility t...
Plasma volume, electrolyte, and endocrine changes during onset of laminitis hypertension in horses. Acute laminitis-hypertension was produced by carbohydrate overloading of the gastrointestinal tract in 12 adult horses. Obel grade 3 (OG3) lameness developed 40 hours (+/- 3.5, SEM) after overfeeding. At OG3 lameness, mean plasma volume was significantly decreased (P less than 0.005) when compared with base-line values. Before OG3 lameness, transient decreases in serum phosphorus and calcium were recorded. Mild hyponatremia also developed before OG3 lameness and persisted. After establishment of OG3 lameness, persistent hypokalemia and increased plasma aldosterone concentration occurred coinci...
Pilar neurocristic hamartoma: its relationship to blue nevus and equine melanotic disease. A unique pigmented lesion, judged to be a hamartoma of neural crest origin, occurring in a female patient, is compared with equine melanotic disease, The characteristic perifollicular arrangement of pigment-laden spindle cells is remarkably similar in both. Previously described patch- and plaque-like blue nevi in humans are also closely related. Light and ultrastructural features showed differentiation toward both nevus cells and Schwann cells, and it is proposed that the lesion be termed pilar neurocristic hamartoma.
A survey of granulomatous and neoplastic diseases of equine skin in north Queensland. A survey of granulomatous and neoplastic diseases of the skin of horses of tropical north Queensland was carried out during the period 1970-1980. Of 338 horses affected, 46.4% suffered from equine fibrosarcoma (sarcoid), 30.2% from phycomycosis, 7.4% from squamous cell carcinoma, 6.8% from other tumours, 4.7% from cutaneous habronemiasis and 4.4% from exuberant granulation tissue. Most specimens were submitted during the first half of the year after the wet monsoonal season and significantly more diseases originated during the first quarter. There was minor variations with breed, age and sex s...
Bioactive and immunoreactive adrenocorticotropin in normal equine pituitary and in pituitary tumors of horses with Cushing’s disease. Equine Cushing's disease is caused by hypersecretion of ACTH by hyperplasia or adenomas of pars intermedia (PI) cells, in contrast to human Cushing's disease, which is caused by hyperplasia or adenomas of pars distalis (PD) ACTH-secreting cells. We assayed both bioactive and immunoreactive (IR) ACTH in two normal equine pituitary glands and in the PD, PI, and pars nervosa of four such glands, as well as in the PI adenomas of five horses with Cushing's disease. In normal horse pituitaries, as in those of other species, most of the bioactive and IR-ACTH was found in PD, much less in PI, and only...
Horse erythrocyte glycoprotein-latex reagent that reacts with infectious mononucleosis heterophile antibody. A sialoglycoprotein from horse erythrocytes was isolated in essentially homogeneous form and found to contain the neuraminidase-sensitive determinant of the horse erythrocyte for Paul-Bunnell heterophile antibodies of infectious mononucleosis. This reactivity was retained after covalent coupling of the antigen to latex particles. The latex reagent has greater stability (greater than 3 years) than either fresh or preserved horse erythrocytes. It can be used in a direct slide test; no absorption of the serum is necessary. The new test compared favorably with some standard tests for infectious mo...
Efficacy of injectable and oral paste formulations of ivermectin against gastrointestinal parasites in ponies. A controlled test was used in ponies to compare the antiparasitic efficacy of ivermectin (22,23-dihydro-avermectin B1) in an injectable micelle solution administered IM with the efficacy of the same drug in an oral paste formulation. Parasite infections were naturally acquired in southern Louisiana. The drug was tested in both formulations at a dosage level of 0.2 mg/kg of body weight. Ivermectin in both formulations tested had an efficacy greater than 98% against Gasterophilus intestinalis and G nasalis larvae. Trichostrongylus axei, Habronema spp, Strongylus vulgaris, S. edentatus, and speci...
Development of horse embryos up to twenty two days after ovulation: observations on fresh specimens. Forty nine embryos, twenty unfertilized eggs and five other fresh eggs of 'doubtful' status have been recovered from 58 pony mares in 122 flushes up to 22 days after ovulation. The fresh egg or embryo recovery rate was 78% with surgical methods (or at slaughter) and 40-60% with non-surgical methods of recovery. The fertilization rate was about 70%. It has been confirmed that horse embryos normally enter the uterus as blastocysts 5-6 days after ovulation. Three features of early embryo morphology have become clearer upon comparison with unfertilized eggs of similar ages; early embryos are often...
