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Topic:Comparative Study
Comparative studies in equine research involve the systematic analysis of different horse breeds, management practices, or physiological responses to identify variations and similarities. These studies are instrumental in understanding how different factors influence health, performance, and behavior in horses. Common areas of comparison include genetic traits, nutritional requirements, disease resistance, and response to training. By evaluating these differences, researchers can develop targeted strategies for breeding, healthcare, and training. This page aggregates peer-reviewed research studies and scholarly articles that focus on the methodologies, findings, and implications of comparative studies in the context of equine science.
Common and species-specific esterases of Equidae–IV. Horse of przewalski, onager and Zebra hartmannae. 1. Among several species of Equidae only E. przewalskii possesses a serum esterase identical with that of E. caballus. 2. The esterases of Hemionidae differ slightly from that of domestic horse by electrophoretic migration and by antigenic structure. 3. Zebras (grevyi, burchelli) appear devoid of this component but Z. hartmannae possesses an esterase of high enzymatic activity, differing notably from that of horse by electrophoretic and antigenic properties.
The ‘normal range’ and precision of phytohaemagglutinin-induced equine lymphocyte transformation in vitro. Data are presented on lymphocyte transformation by phytohaemagglutinin in 20 normal horses. The logarithms of transformation ratios were found to have an approximately normal distribution, giving (for the transformation ratios themselves) a geometric mean of 23.6, a range of 1.92 to 97.3, and an estimated 95 per cent tolerance interval of 1.1 to 488. Analysis of variance on the logarithms of the transformation ratios gave a coefficient of variation of 140 per cent of the transformation ratios themselves for the variation between horses; whereas the coefficient of variation between duplicate sa...
Comparative studies on blood serum alpha-L-fucosidases from several mammalian species. 1. Peripheral blood serum alpha-L-fucosidases have been studied from various mammalian species: Sus scropha var domestica L. (pig), Capra hircus L. (goat), Bos taurus L. (bull, races Morucha and Charolais), Equus caballus L. (horse) and Equus asinus L. (donkey). 2. Fluorimetric and spectrophotometric procedures were used for determination of alpha-L-fucosidases. 3. alpha-L-Fucosidases were more active towards fluorescent substrates than towards chromogenic substrates. 4. pH optima values of the enzymes are: (A) 5.5 for sera from all above-mentioned species when fluorescent substrates were empl...
Polymorphic post-albumin of cattle and horse plasma identified as vitamin D binding protein (Gc protein). Cattle and horse plasma samples of known post-albumin types were radiolabelled with 14C-vitamin D3. These samples were then analysed by polyacrylamide gel electrophoresis, followed by autoradiography. The patterns observed were identical to those of post-albumin variants. The polymorphic post-albumin protein of cattle and horse was thus identified as the vitamin D binding protein and homologous to the Gc protein of human plasma.
Osteochondrosis in the horse. I. A clinical and radiologic investigation of osteochondritis dissecans of the knee and hock joint. The clinical and radiologic features of osteochondritis dissecans in the knee and hock joint of horses are described. The material includes 91 horses, of which 43 had the lesion in one or both knee joints, and 48 in one or both hock joints. It was found that osteochondritis dissecans of the knee joint was more common than the one in the hock joint in thoroughbreds and halfbreds, while the lesion in the hock joint was most common in standardbred trotters. In the knee the lesion was most often located to the lateral trochlear ridge. In the hock joint the predilection site was the intermediate ri...
Genetic polymorphism of the vitamin D binding protein and another post-albumin protein in horse serum. Horizontal polyacrylamide gel electrophoreses, on 10% separation gel, of horse serum revealed polymorphism of the vitamin D binding protein (Gc protein) and another post-albumin protein (Pa). Family data supported the hypothesis that Gc and Pa types were controlled by autosomal codominant alleles. For both Gc and Pa proteins, the homozygous types showed a single fraction while the heterozygous type had two fractions. Pa types were found to be identical to the post-albumin types reported earlier by starch gel electrophoresis. Two Gc alleles, GcF and GcS, and three Pa alleles, Pa D, Pa F and Pa ...
The null allele in the horse esterase (Es) system detected by enzyme assay and rocket immunoelectrophoresis in heterozygous animals. The detection of the recessive null allele of horse serum esterase (Es) is possible in heterozygotes Es+/EsO which by starch gel electrophoresis appear like homozygotes Es+/Es+. Two methods are proposed, the titration of enzymatic activity of esterase and the immunochemical titration of esterase as antigen. These methods can be applied to solve the cases of suspect parentage or in population studies.
Recovery from anaesthesia in ponies: a comparative study of the effects of isoflurane, enflurane, methoxyflurane and halothane. The duration and quality of recovery after separate 2 hour anaesthetic periods with equipotent alveolar concentrations of isoflurane, enflurane, halothane and methoxyflurane were evaluated in experimental ponies. Recovery was shortest after isoflurane anaesthesia, followed by enflurane, halothane and finally methoxyflurane, although standing was achieved more rapidly after enflurane than after isoflurane. This sequence of recovery times was compatible with the respective solubilities of the 4 agents. The smoothest recovery was obtained after isoflurane anaesthesia, followed by methoxyflurane, ...
Bending properties of cortical bone of the horse. Bending properties of samples of cortical bone taken from the cranial, caudal, medial, and lateral quadrants of the midshaft of the radius, femur, and metacarpus of 12 ponies, 18 months old, were determined by 4-point loading at a rate of 10 mm/minute. The elastic modulus for all samples was between 16.2 and 20.2 GN/m2, and the ultimate breaking strength, between 204 and 255 MN/m2. There was greater variation in these properties between bone quadrants than between bones. Samples from the cranial and medial quadrants of both femur and radius were stiffer and had greater ultimate breaking streng...
Regulation of the synthesis of M protein by sugars, Todd Hewitt broth, and horse serum, in growing cells of Streptococcus pyogenes. Various sugars were tested for their effect on the differential rate of synthesis of M protein during the growth of Streptococcus pyogenes strain 0055 M12T12. In a semisynthetic medium alone, a high rate of M protein synthesis occurred with glucose as a substrate; decreasing rates of synthesis occurred with sucrose and trehalose, in that order, although the rates of growth were approximately equal with all sugars. A period of derepressed synthesis of M protein occurred in the lag phase of growth and in the stationary period as the substrates were being depleted. Although glucose inhibited the ...
Analysis of a complex antigenic site on horse cytochrome c. Of the antigenic determinants so far identified for cytochrome c, only one involves more than a single amino acid substitution between the immunogen and host proteins. Both a threonine at position 89 and a glutamic acid at position 92 control one of the three antigenic sites identified in horse cytochrome c, as expressed in rabbits. Three antibody subpopulations, all directed against this region of the molecule, were isolated from the serum of a single rabbit by adsorption on a series of insolubilized cytochromes c. Antibody fluorescence quenching titrations with a variety of cytochromes c wer...
Activity of adenosine deaminase and purine nucleoside phosphorylase in erythrocytes and lymphocytes of man, horse and cattle. 1. Activities of ADA and PNP were measured in erythrocytes and lymphocytes of man, horse and cattle. 2. In bovine hemolysates both enzyme activities are low when compared with activities in human hemolysates. In horse hemolysates both enzyme activities are virtually absent. 3. Enzyme activities are consistently lower (about 50%) in intact lymphocytes than in sonicated lymphocytes. This finding suggests that the uptake of nucleosides is rate-limiting for both enzymes in intact lymphocytes. 4. The activity of ADA in horse lymphocytes is comparable to that in lymphocytes of patients with severe c...
Demonstration of specific antibodies in the central nervous system of horses naturally infected with Borna disease virus. From 18 horses with clinical symptoms of an affection of the central nervous system and with histopathologic alterations in the brain, four were demonstrated to have Bornavirus-specific antibodies. The antibodies are monospecific, recognizing identical antigens from infected brains of different animal species as well as from persistently infected tissue culture cells. Discrete immunoglobulin species (oligoclonal IgG) can be demonstrated in concentrated horse cerebrospinal fluid; they carry Bornavirus antibody specificity. Their presence, together with the higher antibody titers in the cerebros...
Ultrastructural observations suggesting merocrine secretion in the initial segment of the mammalian epididymis. Principal cells in the initial segment of the epididymis in horses, cattle, pigs, sheep, dogs, cats, and rabbits have an abundant, partly rough, endoplasmic reticulum and a large Golgi complex. Small vacuoles with opaque content seem to be formed by the Golgi complex and move to the cell apex, where they empty their contents into the lumen by a merocrine mechanism.
A subunit-sized butyrylcholinesterase present in high concentrations in pooled rabbit serum. A butyrylcholinesterase of mol.wt. approx. 83000 was observed in pooled rabbit serum. The enzyme was named monomeric butyrylcholinesterase to distinguish it from the larger oligomeric butyrylcholinesterase of horse and human serum whose subunits are the same size as the monomeric enzyme. The active-site concentration of monomeric butyrylcholinesterase in the pooled serum was 0.18mum, which is five times the concentration of butyrylcholinesterase in pooled horse serum. This was surprising, since the horse serum is regarded as a rich source of butyrylcholinesterase, whereas rabbit serum is not g...
Vasculature of the equine and canine iris. Methyl methacrylic casts were prepared and tissue was freshly collected for histologic examination to compare the vascular supply and the structure of the vessels of the iris in the adult horse with those of the dog. In the horse, ciliary blood vessels divided in the iris and formed a complete major arterial circle of the iris. In the dog, the ciliary blood vessels divided in the ciliary body and, by means of recurrent branches, formed a complete arterial circle. From this circle, radial arteries extended toward the pupillary margin. The radial vessels in the horse were nearly straight and joi...
Plasma clearance of [51Cr] albumin into the intestinal tract of normal and chronically diarrheal horses. Five clinically normal, mature horses and 11 with chronic diarrhea were given 51Cr-tagged Cohn fraction V equine albumin intravenously. All urine and feces were collected separately, and blood for plasma analysis was taken periodically for 5 to 8 days after injection of the isotope. Plasma clearance of albumin into the intestinal tract of normal horses was calculated as 0.67 +/- 0.23 (SD) ml/kg of body weight/day, with 1.33 +/- 0.69% of the 51Cr dose appearing in the feces in 5 days. Of the 11 diarrheal horses, 8 had a plasma clearance of 0.49 +/- 0.21 ml/kg/day with 1.12 +/- 0.68% of the dose...
Erythrocyte rosette formation of equine peripheral blood lymphocytes. Erythrocyte rosette (ER) formation of equine peripheral blood lymphocytes (PBL) was characterized. Guinea pig and, to a lesser extent, human erythrocytes formed ER; cat, cow, dog, hamster, mouse, rat, and sheep erythrocytes showed negligible rosetting properties. Conditions of the assay were varied to determine which procedure allowed the largest percentage of rosette formation. The PBL from 20 normal horses were then assayed, averaging 38 +/- 2% ER. To characterize the erythrocyte receptor as being on T or B cells, equine thymocytes from 6 foals were assayed; the thymocytes formed an average ...
Staining of glycosaminoglycans in intervertebral disc cells. Disc material from horse, ox, sheep, pig, dog and cat was stained by the Alcian-blue-critical electrolyte concentration technique and with the standard and two-step periodic acid Schiff methods. The effects of pretreatment with hyaluronidase and with chondroitinase was also evaluated. There appears to be a small increase in total cellular glycosaminoglycan content with age in all species: cellular material of high molecular weight however only increases in aged animals. The degree of sulphation of cellular glycosaminoglycans does not vary with age or with position in the disc.
Circular dichroism of porcine, bovine, and equine pancreatic phospholipases A2 and their zymogens. Unusual conformations simulating helix content. Conformation of porcine, bovine, and equine pancreatic phospholipases A2 (EC 3.1.1.4) and their zymogens was studied by the circular dichroism (CD) probe in the far and near ultraviolet spectral zones.
All these phospholipases and their zymogens displayed CD curves suggesting the presence of moderate amounts of α-helical conformation. However, on the basis of known primary structure and recent X-ray structural analysis of prophospholipase A2 crystals (Drenth, J., Enzing, C.M., Kalk, K.H. and Vessies, J.C.A. (1976) Nature 264, 373–377), it has to be concluded that the positive CD band cen...
Studies on a number of erythrocytic enzymes and intermediate products of equine erythrocyte metabolism. The activities and concentrations of a number of erythrocytic enzymes and intermediate products of erythrocyte metabolism were determined in twenty-one normal standard-bred horses which were studied clinically and biochemically. These studies showed that equine anaerobic glycolysis is characterized by a biochemical pattern similar to that observed in human PK deficiency. The greater sensitivity of equine haemoglobin to oxidants is attributable either to low stability of GSH, which may be due either to the low activity of GR or that of 6PGD as observed in the studies. In addition, the saturatio...
[The arterial vessels and their intraosseous course in the leg bones of foals. II. Humerus]. The arterial blood vessels in 24 humeri of equine fetusses and foals are described. The relation between the age and the distribution of these arteries are explained and completely discussed.
Syringomyelia in a thoroughbred foal. The report describes a case of myelodysplasia involing spinal cord segments C7 to T8 of a 5 month old part-Thoroughbred foal. There was a single extensive syringomyelic and hydromyelic cavity which extended from the seventh cervical segment (C7), to the seventh thoracic segment (T7), abnormal size and shape of central canal, and disorderly differentiation of the grey and white matter. The cause of the defect is unknown.
Antigenic relationship between the Tokyo and the Miami strains of equine influenza subtype 2 virus. The first outbreak of equine influenza (EI) infection in Japan was recognized during the period December 1971 to January 1972 [1, 6]. No evidence of the disease had been found before then [2,6]. The etiological agent of this epizootic was identified by hemagglutination-inhibition (HI) and neutralization tests with chicken or ferret antiserum as the subtype 2 of EI virus (6, 7). However, the isolate, A/equine/Tokyo/71 (Tokyo) strain, was not completely identical to the prototypic A/equine/Miami /63 (Miami) strain of the subtype 2, since antibody responses of convalescent horses were 2 to 16 tim...
Immunological properties of two related fragments from human and equine growth hormones. The immunological properties of a synthetic human growth hormone fragment comprising the amino acids 73 through 128 and of the homologous natural horse growth fragment formed by amino acids 73 through 123, have been comparatively studied. Antisera obtained in rabbits inoculated with the native human hormone or with the fragments, were used. By hemagglutination experiments both fragments have the same reactivity toward the anti-human growth hormone serum, but complement fixation curves detect the existence of at least two populations of antibodies presumably originated against the sequence 73-1...
[Fibrinogen level in clinically healthy horses]. Various sets of horses were examined in view of the necessity of widening the range of biochemical substances for diagnostic purposes in the field of veterinary medicine. The aim of the investigations was to obtain basic information on fibrinogen level and its variability. The average value stated was 280 mg% s = 89, sx = 12, V% = 32). The fibrinogen level in horses of the pronounced oxidation type was lower than in those with reduced metabolism, the difference being connected with the speed of sedimentation of erythrocytes. Repeated examination of a part of the set of horses (n = 10) on three...
