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Topic:Diagnosis
Diagnosis in horses involves the systematic identification of diseases and conditions affecting equine health. This process relies on a combination of clinical evaluations, laboratory tests, imaging techniques, and other diagnostic tools to assess the health status of horses. Veterinarians utilize these methods to identify symptoms, determine the underlying causes of health issues, and formulate appropriate treatment plans. Diagnostic procedures in equine medicine can include blood tests, ultrasound, radiography, endoscopy, and more specialized tests such as genetic screening or advanced imaging modalities like MRI and CT scans. This page aggregates peer-reviewed research studies and scholarly articles that explore various diagnostic techniques, their applications, and advancements in the field of equine veterinary medicine.
Ligand binding properties of horse hemoglobins containing deutero- and mesoheme. The reactions of horse globin reconstituted with proto-, deutero-, and mesoheme have been examined by equilibrium and kinetic methods. In virtually all reactions studied, mesohemoglobin displays the more extreme functional behavior, whereas deuterohemoglobin exhibits behavior which is either very similar to native hemoglobin or intermediate between the two. Our kinetic and equilibrium results indicate that the primary effect of heme modification on the functional properties of hemoglobin is to alter the intrinsic reactivities of the deoxy and liganded conformations. Heme modification does not,...
[Values of acid-base equilibrium in the blood of various species of domestic animals]. By means of the Astrup equilibration method the values of the acid-base balance of the blood were determined in 104 cows, 99 horses, 100 pigs, 15 sheep, 20 goats, and in 101 dogs. The pH values of the blood, the partial pressure of CO2, the base excess, the base buffer, the standard bicarbonate, the actual bicarbonate, and the total CO2 were processed statistically and are presented in tables.
Purification, characterization, and quantitation of the antigen employed in the immunodiffusion test for diagnosis of equine infectious anemia. Equine infectious anemia (EIA) antigen extracted from the spleen of horses infected with EIA virus was purified by pH treatment, (NH4)2SO4 fractionation and affinity chromatography. The homogeneity of the antigen was indicated by sedimentation rate and sedimentation equilibrium experiments. A S20,w of 0.51 was determined and a molecular weight of 7600 was calculated from sedimentation equilibrium analysis. The amino acid composition of the pure antigen indicated the antigen is an acidic protein. Employing radical immunodiffusion (RID) and pure antigen a method for quantitating antigen content ...
Cardiac monitoring during exercise tests in the horse. 3. Changes in the electrocardiogram during and after exercise. Changes that occur in the equine ECG during and after exercise have been described and compared with resting ECG's obtained from the same horses. When the speed of work equals or is greater than "three-quarter pace" (i.e. "even time" or 200 metres in 15 seconds) the high heart rates developed cause the loss of ECG waveforms that are readily discernible at rest. Although differences in the waveforms between horses with normal and abnormal resting ECG's have been observed, the more definitive useful information is the fact that abnormal horses showed significantly higher heart rates than normal ...
Squamous cell carcinoma in horses. In a review of neoplasm registry records at Kansas State University (1961 through 1971), 58 squamous cell carcinomas were reported in 10 breeds of horses. Mean age of the affected horses was 12.4 years. The head, eye and ocular adnexa accounted for 43.1%, the external male genitalia, 44.8%, and female perineal region, 12.0% of the squamous cell carcinomas, altogether representing 20.2% of 287 neoplasms recorded.
Condylomata acuminata. A 5-year-old Thoroughbred chestnut mare was presented because of multiple lesions noticed for approximately one year over the vulva and ther perianal areas. Clinically, there were moist warts that stood out because of their whitish color which contrasted strikingly with the surrounding, normally pigmented, uninvolved skin (Fig. 1). Repeated topical applications of 20% podophyllin in 95% ethyl alcohol produced rapid involution. Histopathologically, marked acanthosis, numerous mitoses, prominently vacuolated epidermal cells and a chronic dermal inflammatory infiltrate were seen (Figs. 2, 3, and ...
Transmission of equine infectious anemia virus by Tabanus fuscicostatus. The mechanical transmission of equine infectious anemia (EIA) virus by Tabanus fuscicostatus was investigated. In 1 of 7 transmission trials, a single horsefly transmitted EIA virus from an acutely infected pony to a susceptible pony. Groups of horseflies isolated for 3, 10, or 30 minutes before refeeding transmitted EIA virus, whereas those isolated for 4 or 24 hours did not. Data from field studies indicate that the home range or flight distance of horseflies may exceed 4 miles. That information together with our observations suggest that segregation of infected horses (usually defined as at...
Isolation and partial characterization of three major allergens of horse hair and dandruff. Three major allergens of horse hair and dandruff have been isolated. The fractionation procedures involved various combinations, described in detail, of ethanol precipitation below --5degreesC, cation- and anion-exchange chromatography, and gel filtration. UV absorption, quantitative immunoelectrophoresis and RAST inhibition were used to monitor the separations. Protein impurities constituted less than 5% in all cases. The molecular weights of the isolated proteins were 1.9 X 10(4), 5.1 X 10(4) and 3.1 X 10(4) daltons, respectively. The pIs were determined as 4.1, 3.8 and 3.9, respectively. Th...
Acid phosphatase heterogeneity in horse neutrophil and eosinophil leukocytes. Two distinct groups of acid phosphatase containing granules were characterized in neutrophils, each group displaying different multiple forms of the enzyme. The heavy granule acid phosphatase showed a lysosomal location. A second lighter group of particles contained a thermolabile, thiol-dependent acid p-nitrophenyl and alpha-naphtylphosphatase, an enzyme clearly different from lysosomal acid phosphatase. Acid phosphatase activity from eosinophil leukocytes appeared to be totally associated with the typical eosinophil granules. On mechanical disruption of these particles, an acid phosphatase w...
Purification and characterization of equine infectious anemia virus. EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20 degrees C) of 1.162 and a sedimentation constant of S20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.
Gastrointestinal trichomonads in horses: occurrence and identification. A survey of horses for gastrointestinal trichomonads was conducted to determine the organism's role in equine diarrhea and to establish its proper identity and morphology. Trichomonads were found by cultural examination of feces of 101 (35%) of 289 apparently healthy horses. At necropsy, trichomonads were cultured from 11 (37%) of another 30 horses which showed no signs of diarrhea at the time of death. In 4 of the 11 horses, colonies of trichomonads numbered 30,000 to 150,000/ml of cecal fluid. Diarrhea was induced in 1 of 6 horses, with the fecal fluid containing 10,000 to 110,000 trichomona...
LDH and LDH isoenzymes of synovial fluid in the horse. LDH is an intracellular enzyme, which when cells degenerate is released to the extracellular spaces and body fluids. Cells and organs in the mammalian body differ from each other with respect to their LDH isoenzyme patterns. These circumstances have led to the use of LDH isoenzyme determinations in laboratory diagnostic work. In the present investigation total LDH activity and LDH isoenzyme distribution in equine synovial fluid from healthy joints, joints with serous arthritis, osteochondrosis dissecans and arthrosis, were determined. The fluids from the diseased joints differed from normal sy...
