Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Science (New York, N.Y.)April 16, 1965
Volume 148, Issue 3668 382-383 doi: 10.1126/science.148.3668.382
BENIRSCHKE K, MALOUF N, LOW RJ, HECK H.The chromosome number of the domestic horse is 2n = 64; different races have the same complement. The chromosomes of two Przewalski's horses (at Catskill Game Farm, New York), presumably ancestral wild horses from Mongolia, are identical: 2n = 66, with more acrocentric and fewer metacentric elements than the chromosomes of the domestic horse. This apparent difference in karyotype may help resolve the questions of "purity" in the relatively few remaining Przewalski's horses. Moreover, these findings are of interest in relation to the apparent fertility of hybrids between these species.
GARDIKAS C, KALLINIKOU M, KALLINIKOS G.The present experiments seem to indicate that horse blood is deficient in both Factors VIII and IX, the concentration of Factor VIII varying from 30 to 60 per cent of the normal human levels, and the concentration of Factor IX varying from 50 to 80 per cent, the corresponding concentrations in 12 normal human males varying from 60 to 150 per cent and from 70 to 150 per cent, respectively.
The finding that the recalcification time of horse plasma was considerably longer than that of normal human plasma is of interest, since it is known that only very low concentrations of Factor VIII or Fact...
Tanhuanpää E, Knudsen O.The milk fat from 20 mares on pasture and 9 stable-fed mares was analysed. It is richer in C18:3 than the milk of other domestic species. This was especially marked when the mares were on pasture and were not given any grain. Further, mare’s milk fat is rich in fatty acids containing less than 14 carbon atoms in their chains. Das Milchfett von 20 Stuten auf der Weide und von 9 stallgefütterten Stuten wurde analysiert. Es enthält mehr C18:3 als das Milchfett anderer Haustiere. Dieses war speziell ausgeprägt für Stuten die auf der Weide gingen und kein Getreide bekamen. Das Milchfett der S...
Wilkins PA, Vaala WE, Zivotofsky D, Twitchell ED.Equine leukoencephalomalacia (ELEM) affected 6 of 10 pleasure horses in adjacent paddocks at a boarding facility. Four of the 6 affected horses died or were euthanized. Two of 3 horses presented for treatment survived with complete resolution of clinical signs. Treatment was primarily supportive. Dimethyl sulfoxide, dexamethasone, flunixin meglumine and thiamine were administered as anti-inflammatory agents and to decrease or prevent cerebral edema. Fusarium monileforme was cultured from ear corn fed the affected horses. Fumonisin B1, B2 and B3 were isolated.
Plotka ED, Vevea DN, Eagle TC, Tester JR, Siniff DB.Homogeneous Silastic rods containing ethinylestradiol (EE) (1.5 or 4 g), estradiol-17 beta (E) (4 g) or progesterone (P) (6 g) were implanted into feral mares (Equus caballus) between 4- and 10-yr-old. Six treatment groups (greater than or equal to 10 mares/group) of non-pregnant mares received 36 g P and 12 g E (P+E), 36 g P and 8 g EE (P+HEE), 1.5 g EE (LEE), 3 g EE (MEE, 8 g EE (HEE) or control-implanted mares (CI). CI received implants containing no steroid. Two groups of pregnant mares received P+HEE or HEE. Stallions were placed with the mares 15 to 26 mo after implanting. Blood was coll...
Hisey EA, Ross PJ, Meyers S.As standard in vitro fertilization is not a viable technique in horses yet, many different techniques have been used to create equine embryos for research purposes. One such method is parthenogenesis in which an oocyte is induced to mature into an embryo-like state without the introduction of a spermatozoon, and thus they are not considered true embryos. Another method is somatic cell nuclear transfer (SCNT), in which a somatic cell nucleus from an extant horse is inserted into an enucleated oocyte, creating a genetic clone of the donor horse. Due to limited availability of equine oocytes in t...
King N, Suleiman MS.Exercise induces significant changes in the free intracellular amino acid pool in skeletal muscle but little is known of whether such changes also occur in cardiac muscle. In this study the effect of regular exercise on the size and the constituents of the free amino acid pool in the hearts and in the plasma of thoroughbred horses was investigated. The total free intracellular amino acid pool in the hearts of control horses was 30.9 +/- 1.2 mumol/g wet weight (n = 6). Glutamine but not taurine was present at the highest concentration (13.5 +/- 0.9 and 7.7 +/- 0.69 mumol/g wet weight for glutam...
van den Hoven R, Meijer AE, Wensing T, Breukink HJ.Gluteal muscle specimens were taken from 4 horses. From 1 of the 4 gluteal muscles, serial sections were prepared. Individual muscle fibers were identified and studied, using photomicrographs of sections stained by different enzyme histochemical methods. In specimens in which cytoplasmic soluble enzymes were studied, use was made of the semi-permeable membrane technique to hamper enzyme diffusion into reaction fluids. Enzymes involved in glycogenolysis, glycolysis, the tricarboxylic acid cycle, synthesis of reduced nicotinamide adenine dinucleotide phosphate, the pentose phosphate cycle, the a...
Kirker-Head CA, Kerwin PJ, Steckel RR, Rubin CT.Liquid metal strain gauges (LMSG) were implanted surgically at three locations on the intact linea alba (LA) in eight horses. LA strain, strain rate, change in strain and stress were recorded during general anaesthesia, recovery from anaesthesia, standing, vocalisation, rectal palpation and at the walk, trot and canter. LA stress was quantitated using an in vitro tensiometric technique. Stress-strain responses differed significantly (P < 0.05) with location, but each described a characteristic relationship for viscoelastic tissues. Maximum peak stress, peak strain and change in strain occurred...
Raz T, Carley S, Card C.The objective was to compare the effects of eFSH and deslorelin treatment regimes on ovarian stimulation and embryo production of donor mares in early spring transition. Starting January 30th, mares kept under ambient light were examined by transrectal ultrasonography. When a follicle > or =25 mm was detected, mares were assigned to one of two treatment groups, using a sequential alternating treatment design. In the eFSH group, mares (n=18) were treated twice daily with eFSH (12.5mg im) until they achieved a follicle > or =35 mm; hCG was given 36 h later. In the deslorelin group, mares (n=18) ...
Dehennin L, Bonnaire Y, Plou P.The dietary supplements 19-norandrostenedione and 19-norandrostenediol are potential metabolic precursors of nandrolone. They are considered by law in the United States as prohormones without proven therapeutic, curative or diagnostic properties, and therefore available as over-the-counter drugs. Oral dosages of 0.1-1 mg/kg body weight were readily absorbed in the equine intestinal tract and thereby led to urinary excretion of drastically increased 5alpha-estrane-3beta,17alpha-diol conjugates, which are known to be final metabolites of nandrolone. The actual rules for detection of illicit nand...
Merlo B, Del Prete C, Mari G, Iacono E.The demand for equine in vitro produced embryos has increased over the last decade. The aim of this study was to compare the effects of an extended IVM or a prolonged period before fertilization, including holding time, on equine immature oocyte developmental competence. Oocytes, collected from abattoir-derived ovaries, were divided into 4 groups: H0/24 (n = 165) 0 h holding + standard 24-26 h IVM; H8/36 (n = 160) 8 h holding + 36 h IVM; H20/24 (n = 187) 20 h holding + 24 h IVM; H0/44 (n = 164) 0 h holding + 44 h IVM. Oocytes matured to MII were fertilized by intracytoplasm...
Tomić S, Sesartić L, Tomasić J.1. 14C-labelled methyl 2,6-di-O-pivaloyl-alpha-D-glucopyranoside (1) was used as a substrate for esterases from rabbit, guinea pig, mouse, donkey, pig, horse, sheep and human sera. 2. Stepwise de-esterification of the diester substrate 1 occurred with rabbit, guinea pig and mouse serum. Data on time-course experiments and kinetic data are reported. 3. The use of donkey, pig, horse, sheep and human serum led to the migration of the 2-O-pivaloyl group in substrate 1 to the position 4- in the sugar molecule, followed by stepwise de-esterifications of both 1 and the newly formed methyl 4,6-di-O-pi...
Yamamoto M, Yamamoto I, Tanaka Y, Sugano M.1. A method for the purification of horse serum lecithin:cholesterol acyltransferase has been established. 2. The method involves the adsorption of the enzyme from diluted horse serum on DEAE-Sephadex A-50, (NH4)2SO4 fractionation, 1-butanol treatment, and chromatographic techniques of DEAE-Sepharose CL-6B, DEAE-Sephadex A-50, Affi-Gel blue and hydroxylapatite. 3. The resultant enzyme preparation essentially formed a single main band when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. 4. The final purification of the enzyme was 20,000-fold with 7% yi...
Naugle R, Grams K.The development of sophisticated delivery equipment, as well as safer and more effective drugs, has made remote delivery of animal drugs a standard and readily available tool for wildlife professionals, veterinarians, ranchers, and animal control officers. In the 1980s, researchers began treating a wide variety of wildlife with injectable porcine zona pellucida immunocontraceptive vaccines. Remote delivery of immunocontraceptives has been proven effective at the individual and population level for wild horses and urban deer. However, it took only a short time at each study site to understand t...
Kampmann S, Hampson BA, Pollitt CC.Recent record rainfall in much of semi-arid Central Australia is the most likely reason for a feral horse population increase in excess of normal. Uncontrolled numbers of feral horses have habitat degradation and animal welfare implications. Objective: The aims of this study were to investigate the social structure of feral horses and assess their population growth rate following unseasonably high rainfall. Methods: The study area was 4000 km(2) of unmanaged, semi-arid country in Central Australia (latitude 24.50°S, longitude 132.10°E). Horses were identified by descriptive features from g...
Griffin PG, Ginther OJ.Fetal activity and mobility and changes in diameter of the allantoic fluid compartment in the uterine horns were studied in mares between days 69 and 81 of pregnancy by use of transrectal ultrasonography (n = 12) and transcervical videoendoscopy (n = 8). The insertion tube of the videoendoscope was positioned within the allantoic sac to permit viewing of the fetus and entrance to each uterine horn. Each uterine horn was divided ultrasonographically into 3 segments of equal length, and the horns were designated on the basis of side of umbilical attachment (cord vs noncord horns). The diameter o...
Pinchbeck GL, Clegg PD, Proudman CJ, Morgan KL, French NP.A concurrent case-control study of 12 UK racecourses was made between March 1, 2000, and August 31, 2001, to identify and quantify the factors associated with the risk of horses falling in steeplechase races. Cases were defined as a jumping effort at a steeplechase fence that resulted in a fall and controls were defined as a successful jumping effort over any steeplechase fence at any of the 12 racecourses within 14 days before or after the case fall. Information on the horse, the jockey and the race were collected and all the fences on all the courses were surveyed. Conditional logistic regre...
Ball BA, Brinsko SP, Thomas PG, Miller PG, Ellington JE.Development of 1- to 2-cell in vivo fertilized equine embryos cultured with or without uterine tubal epithelial cells (UTEC) was studied. One- to 2-cell embryos (n = 26) were collected surgically from the uterine tubes of pony mares 1 day after ovulation. Four- to 8-cell embryos (n = 9) were collected 2 days after ovulation. Presumptive zygotes and 2-cell embryos were cultured with (n = 17) or without (n = 9) UTEC, and all 4- to 8-cell embryos were cocultured with UTEC as positive controls. Uterine tubal epithelial cells were used as cell suspensions within 2 weeks after initiation of cultures...
Withrow JM, Sargent GF, Scheffrahn NS, Kesler DJ.Two pony mares were administered 150 mg of testosterone propionate every other day for 20 days (ten injections) and every ten days there-after. An additional two mares and one stallion were not treated and served as controls. Testosterone propionate was dissolved in absolute ethanol and administered subcutaneously. Sex behavior tests were conducted 26 and 40 days after the first injection. Control mares exhibited very little male sex behavior. Both testosterone propionatetreated mares, however, exhibited mounting, sniffing, flehmen, biting and vocalization behavior in the presence of an estrou...
Decloedt AI, Bailly-Chouriberry L, Vanden Bussche J, Garcia P, Popot MA, Bonnaire Y, Vanhaecke L.To ensure fair competition and to protect the horse's welfare, horses have to compete on their own merits, without any unfair advantage that might follow the use of drugs. Therefore, regulatory authorities list all substances that are not allowed in competition, including most anabolic-androgenic steroids. As zero-tolerance is retained, the question arose whether the consumption of mouldy feed could lead to the excretion of steroids, due to the biotransformation of plant phytosterols to steroids. A rapid ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analyt...
Caron JP, Eberhart SW, Nachreiner R.Articular cartilage explants from 3 horses were maintained in tissue culture to test the effects of a polysulfated glycosaminoglycan on proteoglycan biosynthesis. Cultures were exposed to concentrations of 0, 50, or 200 micrograms of the drug/ml for either 2 days or 6 days, and labeled with 35S, before measuring the content of sulfated proteoglycan in the culture media and in extracts of cartilage. In a second experiment, the explants were incubated with the isotope and subsequently exposed to the same concentrations of the polysulfated glycosaminoglycan for 4 days. Subsequently, the amount of...
Wagner IP, Hood DM.To evaluate the effect of prolonged water exposure on tissue mass and solutes of outer and inner layers of the stratum medium, sole, frog, and the stratum medium (SMZA) zona alba layer of horses' hooves. SPECIMEN POPULATION: 10 hooves from 10 horses without foot abnormalities. Methods: Hoof wall tissue specimens were obtained and immersed for 10 days in distilled deionized water. Serial changes in mass were recorded during the immersion period. Subsequently, osmolarity and Na+, K, Cl-, and protein concentrations of the immersion solution were quantified. Results: Fully cornified outer hoof wal...
Mazan MR.The airways are the first part of the pathway in the oxygen transport chain that is critical to excellent athletic performance, and the lower airways are considered the final gatekeeper before oxygen enters the blood and carbon dioxide exits. Horses are blessed with large airways and lungs that allow them to be superb athletes, but the down side of this largesse on the part of evolution is that unless they are truly elite athletes they may withstand noninfectious disease of the lower respiratory tract for months to years before the owner or trainer notices. The two conditions of the lower resp...
Chen CL, Neilson JT, Kumar MS, Estes KS.Highly purified equine prolactin was prepared from equine pituitary glands (hypophysis) by serial extractions with water at pH 5.5, 0.1 M (NH4)2SO4 at pH 4.0, and 0.25 M (NH4)2SO4 at pH 5.5 to remove other hormones, and then finally with 70% ethanol at pH 9.3 to 10.0 to extract prolactin. Preliminary purification of the extract involved salting out other substances with 0.1% NaCl at pH 9.0. Prolactin was precipitated out by adding three times the volume of 95% ethanol at 4 C. This prolactin preparation had a biological potency of 24 IU/mg. Further purification by isoelectric focusing on a pH g...
White KL, Thomson DL, Wood TC.An indirect immunofluorescence assay was used to detect the presence of H-Y antigen on equine blastocysts. A total of 33 blastocyst stage horse embryos were collected 6 to 7 days post-ovulation by trans-cervical flush and were immediately evaluated for the presence of H-Y antigen. Additionally, 17 embryos, were collected and cultured for 72 h to the expanded blastocyst stage and similarly evaluated. Embryos were placed in medium containing monoclonal antibodies to H-Y antigen followed by incubation in medium containing 1/10 (v/v) fluorescein isothiocyanate conjugated goat anti-mouse IgM Fc spe...
Vos JH, Deleu SA, Heling W, de Vries AR, Zeeuwen AA.The most relevant results of a written questionnaire among veterinarians in the Netherlands are presented and discussed. The inquiry was performed by MarketResponse Nederland BV. The objective was to get an overview of the current perception and the future view of the profession. The response was 37%. Most respondents (66%) were practitioners, 7% were active in research or teaching institutions, 5% were governmental employees, 5% were employed in industry, and 17% did not belong to any of these categories (retired, unemployed etc.). Forty-seven per cent of the veterinarians practised mainly sm...
Gethmann D.In the early twentieth century, counting and speaking horses, like the famous Clever Hans or the "Horses of Elberfeld," became widely debated subjects in experimental psychology. The idea was to determine whether their learning success was only a fraud, or if it might open up a new chapter in "animal psychology" - or even belong to the realm of parapsychology and telepathy. When their tricks were discovered, the teachers of the animals were marked as charlatans. Both the attempts to detect charlatans and the efforts to avoid this accusation during the talking horse experiments proceeded using ...
Hallebeek JM, Beynen AC.In a Latin square design, six horses were fed hay and concentrates with isoenergetic amounts of either starch, cellulose or medium chain triacylglycerols (MCT). The dietary variables provided on average 22% of total dietary net energy. Plasma triacylglycerols and other variables of lipid metabolism were determined. The experimental periods lasted 21 days. Blood samples were taken just before the morning meal and three and six hours later. The diet rich in MCT significantly raised the plasma level of triacylglycerols when compared to either the starch- or cellulose-rich diet. The plasma concent...
Ingarden J, Dubiel A, Rauluszkiewicz S.This study investigated the effect of a beta blocker on parturition, fetal membrane retention and subsequent fertility in mares. The animals were divided into an experimental group (150 mares treated during second stage labour with propranolol [50 mg i.v.]) and a control group (180 mares with no treatment). Administration of propranolol was associated with a significant decrease in time of expulsion of fetal membranes, and an increase in both exhibition of foal heat and pregnancy rates at this heat.
Drum MG, Les CM, Park RD, Norrdin RW, McIlwraith CW, Kawcak CE.The purpose of this study was to compare subchondral bone density obtained using quantitative computed tomography with ash density values from intact equine joints, and to determine if there are measurable anatomic variations in mean subchondral bone density. Five adult equine metacarpophalangeal joints were scanned with computed tomography (CT), disarticulated, and four 1-cm(3) regions of interest (ROI) cut from the distal third metacarpal bone. Bone cubes were ashed, and percent mineralization and ash density were recorded. Three-dimensional models were created of the distal third metacarpal...
Salinger A, Mochal-King C, Clinton K, Priddy LB, Elder S, Fontenot R, Eddy A, Wills R, Jaffe M.Frontal plane slab fractures account for the majority of third carpal bone (C3) fractures in racing and performance horses. Recommended treatment is stabilization with a lagged AO cortical screw. Associated complications are fragment splitting, fragment spinning, and irritation of dorsal soft tissue structures. A novel, headless, cannulated screw with interlocking threads the Headless Compression Screw Fastener (HCSF) has been developed to resist multidirectional forces and bending moments; however, it has not been applied in the horse. Simulated C3 frontal plane slab fractures were created in...
Illera JC, Illera M, Hamlin RL.Electrocardiograms were obtained from 100 male crossbred horses. In addition to standard limb leads, leads were taken from 12 points on the thorax. The QRS complexes were categorized into 1 of 8 forms on the basis of configuration and amplitudes of component deflections. Relative uniformity of QRS was determined for each lead, using the percentage of horses that possess a particular configuration. All unipolar thoracic leads, except those coplanar with the frontal plane, and lead aVR had great uniformity, with greater than 80% of the horses with a QRS of similar contour.
