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Topic:Imaging Techniques

Imaging techniques in horses encompass a range of diagnostic tools used to visualize the internal structures and systems of equine patients. These techniques assist in the diagnosis, monitoring, and treatment planning of various conditions affecting horses. Common imaging modalities include radiography (X-rays), ultrasonography, magnetic resonance imaging (MRI), and computed tomography (CT). Each technique offers unique advantages in terms of resolution, depth of penetration, and the type of tissues that can be examined. For instance, radiography is frequently used for assessing bone structures, while ultrasonography is often employed for soft tissue evaluation. MRI and CT provide detailed cross-sectional images, aiding in the assessment of complex anatomical regions. This page compiles peer-reviewed research studies and scholarly articles that explore the application, advancements, and comparative efficacy of imaging techniques in equine veterinary medicine.
Longitudinal Cell Tracking and Simultaneous Monitoring of Tissue Regeneration after Cell Treatment of Natural Tendon Disease by Low-Field Magnetic Resonance Imaging.
Stem cells international    January 10, 2016   Volume 2016 1207190 doi: 10.1155/2016/1207190
Berner D, Brehm W, Gerlach K, Gittel C, Offhaus J, Paebst F, Scharner D, Burk J.Treatment of tendon disease with multipotent mesenchymal stromal cells (MSC) is a promising option to improve tissue regeneration. To elucidate the mechanisms by which MSC support regeneration, longitudinal tracking of MSC labelled with superparamagnetic iron oxide (SPIO) by magnetic resonance imaging (MRI) could provide important insight. Nine equine patients suffering from tendon disease were treated with SPIO-labelled or nonlabelled allogeneic umbilical cord-derived MSC by local injection. Labelling of MSC was confirmed by microscopy and MRI. All animals were subjected to clinical, ultrason...
Addition of Mesenchymal Stem Cells to Autologous Platelet-Enhanced Fibrin Scaffolds in Chondral Defects: Does It Enhance Repair?
The Journal of bone and joint surgery. American volume    January 8, 2016   Volume 98, Issue 1 23-34 doi: 10.2106/JBJS.O.00407
Goodrich LR, Chen AC, Werpy NM, Williams AA, Kisiday JD, Su AW, Cory E, Morley PS, McIlwraith CW, Sah RL, Chu CR.The chondrogenic potential of culture-expanded bone-marrow-derived mesenchymal stem cells (BMDMSCs) is well described. Numerous studies have also shown enhanced repair when BMDMSCs, scaffolds, and growth factors are placed into chondral defects. Platelets provide a rich milieu of growth factors and, along with fibrin, are readily available for clinical use. The objective of this study was to determine if the addition of BMDMSCs to an autologous platelet-enriched fibrin (APEF) scaffold enhances chondral repair compared with APEF alone. Methods: A 15-mm-diameter full-thickness chondral defect wa...
High-throughput analysis of horse sperms’ 3D swimming patterns using computational on-chip imaging.
Animal reproduction science    January 7, 2016   Volume 169 45-55 doi: 10.1016/j.anireprosci.2015.12.012
Su TW, Choi I, Feng J, Huang K, Ozcan A.Using a high-throughput optical tracking technique that is based on partially-coherent digital in-line holography, here we report a detailed analysis of the statistical behavior of horse sperms' three-dimensional (3D) swimming dynamics. This dual-color and dual-angle lensfree imaging platform enables us to track individual 3D trajectories of ∼1000 horse sperms at sub-micron level within a sample volume of ∼9μL at a frame rate of 143 frames per second (FPS) and collect thousands of sperm trajectories within a few hours for statistical analysis of their 3D dynamics. Using this high-throughp...
Assessment of distribution of ventilation by electrical impedance tomography in standing horses.
Physiological measurement    December 29, 2015   Volume 37, Issue 2 175-186 doi: 10.1088/0967-3334/37/2/175
Ambrisko TD, Schramel JP, Adler A, Kutasi O, Makra Z, Moens YP.The aim was to evaluate the feasibility of using electrical impedance tomography (EIT) in horses. Thoracic EIT was used in nine horses. Thoracic and abdominal circumference changes were also measured with respiratory ultrasound plethysmography (RUP). Data were recorded during baseline, rebreathing of CO2 and sedation. Three breaths were selected for analysis from each recording. During baseline breathing, horses regularly took single large breaths (sighs), which were also analysed. Functional EIT images were created using standard deviations (SD) of pixel signals and correlation coefficients (...
Quantitative comparison of bone mineral density characteristics of the distal epiphysis of third metacarpal bones from Thoroughbred racehorses with or without condylar fracture.
American journal of veterinary research    December 29, 2015   Volume 77, Issue 1 32-38 doi: 10.2460/ajvr.77.1.32
Bogers SH, Rogers CW, Bolwell C, Roe W, Gee E, McIlwraith CW.To compare regional proportions and spatial distributions of volumetric bone mineral density (BMDv) of the palmar aspect of the distal epiphysis of the third metacarpal bone (McIII) in limbs with or without a condylar fracture from Thoroughbred racehorses. Methods: McIIIs from cadavers of Thoroughbred racehorses with (n = 6 bones) and without (8) a condylar fracture. Methods: BMDv and spatial distributions of BMDv in peripheral quantitative CT images of the distal epiphysis of McIIIs were quantitatively assessed with spatial analysis software. Relative proportions of voxels within 9 threshold ...
Diagnostic Imaging of the Lower Respiratory Tract in Neonatal Foals: Radiography and Computed Tomography.
The Veterinary clinics of North America. Equine practice    November 28, 2015   Volume 31, Issue 3 497-514 doi: 10.1016/j.cveq.2015.08.003
Lascola KM, Joslyn S.Diagnostic imaging plays an essential role in the diagnosis and monitoring of lower respiratory disease in neonatal foals. Radiography is most widely available to equine practitioners and is the primary modality that has been used for the characterization of respiratory disease in foals. Computed tomography imaging, although still limited in availability to the general practitioner, offers advantages over radiography and has been used diagnostically in neonatal foals with respiratory disease. Recognition of appropriate imaging protocols and patient-associated artifacts is critical for accurate...
Radiographic, computed tomographic and surgical anatomy of the equine sphenopalatine sinus in normal and diseased horses.
Equine veterinary journal    October 9, 2015   Volume 48, Issue 5 578-584 doi: 10.1111/evj.12492
Tucker R, Windley ZE, Abernethy AD, Witte TH, Fiske-Jackson AR, Turner S, Smith LJ, Perkins JD.Knowledge of imaging anatomy, surgical anatomy and disorders affecting the sphenopalatine sinus are currently lacking. Objective: To describe the computed tomographic (CT) and surgical anatomy of the sphenopalatine sinus and diagnosis, treatment and outcome in clinical cases with sphenopalatine sinus disease. Methods: Cadaver observational study and retrospective case series. Methods: The sphenopalatine sinuses of 10 normal cadaver heads were examined with digital radiography, CT and sinoscopic examination prior to anatomical sectioning. Sphenopalatine sinus anatomy was described and compared ...
Seizures in horses: diagnosis and classification.
Veterinary medicine (Auckland, N.Z.)    October 5, 2015   Volume 6 301-308 doi: 10.2147/VMRR.S62078
Lacombe VA.Seizures are a diverse and very common set of chronic neurologic disorders in humans and dogs but are less common in horses. Seizures refer to a specific clinical event (described as sudden and severe) regardless of the etiology, which includes both intracranial and extracranial causes. Therefore, after briefly reviewing some definitions, this article aims to describe the use of a standardized classification, which could facilitate a logical approach for the clinician to establish a diagnosis, as well as to use a consistent mode of communication. For instance, seizures can be classified by typ...
Radiographic characterization of ossification of the ungular cartilages in horses: 271 cases (2005-2012).
Journal of the American Veterinary Medical Association    September 19, 2015   Volume 247, Issue 7 801-811 doi: 10.2460/javma.247.7.801
Jones LE, Dyson SJ.To describe in detail the radiographic appearance of ossified ungular cartilages in horses and to evaluate the usefulness of a flexed dorsolateral-palmaromedial oblique (flexed oblique) radiographic view in evaluating the ungular cartilages. Methods: Retrospective case series. Sample-Radiographs of 1,255 front feet of horses. Methods: Ossification of the ungular cartilages was graded during evaluation of radiographs on a scale from 0 to 5; feet with cartilages with an ossification grade ≥ 2 were included (386 feet [271 horses]). The shape (straight, curved outward or inward, and dorsopalmar ...
Clinical Research Abstracts of the British Equine Veterinary Association Congress 2015.
Equine veterinary journal    September 17, 2015   Volume 47 Suppl 48 13-14 doi: 10.1111/evj.12486_30
Satchell G, McGrath M, Dixon J, Pfau T, Weller R.Infrared imaging is becoming popular as an aid to traditional diagnostic tools, its repeatability under practically relevant conditions is still discussed. Objective: We hypothesise that time of day, ambient temperature and relative humidity affect thermography readings, and that there is no difference between values from left and right side. Methods: Repeated thermography in a convenience sample. Methods: Fifteen sound horses were thermographically imaged at 3 time points in the same day (08.00, 12.00 and 16.00 h). Images were taken of the following regions: neck, carpus, distolateral thora...
Endobronchial Ultrasound Reliably Quantifies Airway Smooth Muscle Remodeling in an Equine Asthma Model.
PloS one    September 8, 2015   Volume 10, Issue 9 e0136284 doi: 10.1371/journal.pone.0136284
Bullone M, Beauchamp G, Godbout M, Martin JG, Lavoie JP.Endobronchial ultrasonography (EBUS) revealed differences in the thickness of the layer representing subepithelial tissues (L2) between human asthmatics and controls, but whether this measurement correlates with airway smooth muscle (ASM) remodeling in asthma is unknown. In this study, we sought to determine the ability of EBUS to predict histological ASM remodeling in normal and equine asthmatic airways. We studied 109 isolated bronchi from the lungs of 13 horses. They underwent EBUS examination using a 30 MHz radial probe before being processed for histology. ASM remodeling parameters were e...
Ultrasonography detects early laryngeal muscle atrophy in an equine neurectomy model.
Muscle & nerve    August 1, 2015   Volume 53, Issue 4 583-592 doi: 10.1002/mus.24785
Chalmers HJ, Caswell J, Perkins J, Goodwin D, Viel L, Ducharme NG, Piercy RJ.A unilateral neurectomy model was used to study the relationship between histologic and ultrasonographic tissue characteristics during muscle atrophy over time. Methods: This investigation was an in vivo experimental study in an equine model (n = 28). Mean pixel intensity of ultrasonographic images was measured, a muscle appearance grade was assigned weekly, and muscles were harvested from 4 to 32 weeks. Minimum fiber diameter, fiber density per unit area, percent collagen, percent fat, and fiber type profile were measured from muscle cryosections and correlated with the ultrasonographic param...
Radiographic and Computed Tomographic Configuration of Incomplete Proximal Fractures of the Proximal Phalanx in Horses Not Used for Racing.
Veterinary surgery : VS    July 21, 2015   Volume 44, Issue 7 809-815 doi: 10.1111/vsu.12364
Brünisholz HP, Hagen R, Fürst AE, Kuemmerle JM.To characterize the configuration of incomplete proximal fractures of the proximal phalanx (P1) in horses not used for racing and compare radiographic with computed tomography (CT) findings. Methods: Historical cohort. Methods: Twenty-four horses with incomplete fractures of P1. Methods: Medical records of horses not used for racing diagnosed with an incomplete proximal fracture of P1 based on clinical and radiographic examination and confirmed by CT between 2008 and 2013 were retrieved. Radiographs and CT studies of these horses were analyzed using a subjective grading system and by measuring...
X-ray boost for Vets with Horsepower.
The Veterinary record    July 18, 2015   Volume 177, Issue 3 62 doi: 10.1136/vr.h3664
No abstract available
Evaluation of a Novel Screw Position in a Type III Distal Phalanx Fracture Model: An Ex Vivo Study.
Veterinary surgery : VS    July 4, 2015   Volume 44, Issue 7 829-837 doi: 10.1111/vsu.12354
Bindler D, Koch C, Gendron K, Ferguson SJ, Kaposi AD, Papp M, Bodó G.Mechanical evaluation of a novel screw position used for repair in a type III distal phalanx fracture model and assessment of solar canal penetration (SCP). Methods: Experimental study. Methods: Disarticulated equine hooves (n = 24) and 24 isolated distal phalanges. Methods: Hooves/distal phalanges cut in a sagittal plane were repaired with 1 of 2 different cortical screw placements in lag fashion. In group 1 (conventional screw placement), the screw was inserted halfway between the proximal border of the solar canal (SC) and the subchondral bone surface on a line parallel to the dorsal co...
Angiographic Variation of the Internal Carotid Artery and its Branches in Horses.
Veterinary surgery : VS    July 1, 2015   Volume 44, Issue 6 784-789 doi: 10.1111/vsu.12357
Khairuddin NH, Sullivan M, Pollock PJ.To record the angiographic anatomy of the equine internal carotid artery (ICA) using angiography techniques. Methods: In vitro descriptive study. Methods: Equine cadaver specimens (n = 50). Methods: Head and neck specimens from horses of mixed breed, age, sex, and use without a history of guttural pouch disease had carotid and cerebral angiography using conventional (n = 7) and rotational angiography (43). Angiographic findings were verified by arterial latex casts. Results: Variation in ICA anatomy was categorized into 4 groups: (1) the internal carotid and occipital arteries arising ...
Computed Tomographic Tenography of Normal Equine Digital Flexor Tendon Sheath: An Ex Vivo Study.
Veterinary medicine international    June 22, 2015   Volume 2015 903169 doi: 10.1155/2015/903169
Aim of this study was to document the normal computed tomographic tenography findings of digital flexor tendon sheath. Six ex vivo normal equine forelimbs were used. An axial approach was used to inject 185 mg/mL of iopamidol in a total volume of 60 mL into the digital flexor tendon sheaths. Single-slice helical scans, with 5 mm thickness, spaced every 3 mm, for a pitch of 0.6, and with bone algorithm reconstruction, were performed before and after injections of contrast medium. To obtain better image quality for multiplanar reconstruction and 3D reformatting, postprocessing retrorecon...
Estimation of articular cartilage properties using multivariate analysis of optical coherence tomography signal.
Osteoarthritis and cartilage    June 6, 2015   Volume 23, Issue 12 2206-2213 doi: 10.1016/j.joca.2015.05.034
Puhakka PH, Te Moller NCR, Afara IO, Mäkelä JTA, Tiitu V, Korhonen RK, Brommer H, Virén T, Jurvelin JS, Töyräs J.The aim was to investigate the applicability of multivariate analysis of optical coherence tomography (OCT) information for determining structural integrity, composition and mechanical properties of articular cartilage. Methods: Equine osteochondral samples (N = 65) were imaged with OCT, and their total attenuation and backscattering coefficients (μt and μb) were measured. Subsequently, the Mankin score, optical density (OD) describing the fixed charge density, light absorbance in amide I region (Aamide), collagen orientation, permeability, fibril network modulus (Ef) and non-fibrillar mat...
Determining collagen distribution in articular cartilage using contrast-enhanced micro-computed tomography.
Osteoarthritis and cartilage    May 21, 2015   Volume 23, Issue 9 1613-1621 doi: 10.1016/j.joca.2015.05.004
Nieminen HJ, Ylitalo T, Karhula S, Suuronen JP, Kauppinen S, Serimaa R, Hæggström E, Pritzker KP, Valkealahti M, Lehenkari P, Finnilä M....Collagen distribution within articular cartilage (AC) is typically evaluated from histological sections, e.g., using collagen staining and light microscopy (LM). Unfortunately, all techniques based on histological sections are time-consuming, destructive, and without extraordinary effort, limited to two dimensions. This study investigates whether phosphotungstic acid (PTA) and phosphomolybdic acid (PMA), two collagen-specific markers and X-ray absorbers, could (1) produce contrast for AC X-ray imaging or (2) be used to detect collagen distribution within AC. Methods: We labeled equine AC sampl...
Ultrasonographic detection of early atrophy of the intrinsic laryngeal muscles of horses.
American journal of veterinary research    April 25, 2015   Volume 76, Issue 5 426-436 doi: 10.2460/ajvr.76.5.426
Chalmers HJ, Viel L, Caswell JL, Ducharme N.To describe the ultrasonographic changes in the cricoarytenoideus dorsalis (CAD) and cricoarytenoideus lateralis (CAL) muscles of horses before and at various times during the 32 weeks after unilateral neurectomy of the right recurrent laryngeal nerve. Methods: 28 healthy Standardbreds. Methods: For each horse, the appearance of the CAD and CAL muscles on the right (neurectomized) and left (control) sides was serially monitored ultrasonographically by percutaneous (CAD and CAL) and transesophageal (CAD) approaches. The ultrasonographic images were assessed to determine the mean pixel intensity...
A mechanostatistical approach to cortical bone remodelling: an equine model.
Biomechanics and modeling in mechanobiology    April 11, 2015   Volume 15, Issue 1 29-42 doi: 10.1007/s10237-015-0669-x
Wang X, Thomas CD, Clement JG, Das R, Davies H, Fernandez JW.In this study, the development of a mechanostatistical model of three-dimensional cortical bone remodelling informed with in vivo equine data is presented. The equine model was chosen as it is highly translational to the human condition due to similar Haversian systems, availability of in vivo bone strain and biomarker data, and furthermore, equine models are recommended by the US Federal Drugs Administration for comparative joint research. The model was derived from micro-computed tomography imaged specimens taken from the equine third metacarpal bone, and the Frost-based 'mechanostat' was in...
ANATOMICAL STUDY OF CRANIAL NERVE EMERGENCE AND SKULL FORAMINA IN THE HORSE USING MAGNETIC RESONANCE IMAGING AND COMPUTED TOMOGRAPHY. Gonçalves R, Malalana F, McConnell JF, Maddox T.For accurate interpretation of magnetic resonance (MR) images of the equine brain, knowledge of the normal cross-sectional anatomy of the brain and associated structures (such as the cranial nerves) is essential. The purpose of this prospective cadaver study was to describe and compare MRI and computed tomography (CT) anatomy of cranial nerves' origins and associated skull foramina in a sample of five horses. All horses were presented for euthanasia for reasons unrelated to the head. Heads were collected posteuthanasia and T2-weighted MR images were obtained in the transverse, sagittal, and do...
Magnetic resonance imaging of an equine fracture model containing stainless steel metal implants.
Equine veterinary journal    March 15, 2015   Volume 48, Issue 3 321-325 doi: 10.1111/evj.12424
Pownder SL, Koff MF, Shah PH, Fortier LA, Potter HG.Post operative imaging in subjects with orthopaedic implants is challenging across all modalities. Magnetic resonance imaging (MRI) is preferred to assess human post operative musculoskeletal complications, as soft tissue and bones are evaluated without using ionising radiation. However, with conventional MRI pulse sequences, metal creates susceptibility artefact that distorts anatomy. Assessment of the post operative equine patient is arguably more challenging due to the volume of metal present, and MRI is often not performed in horses with implants. Novel pulse sequences such as multiacquisi...
Role of subchondral bone remodelling in collapse of the articular surface of Thoroughbred racehorses with palmar osteochondral disease.
Equine veterinary journal    March 12, 2015   Volume 48, Issue 2 228-233 doi: 10.1111/evj.12415
Bani Hassan E, Mirams M, Ghasem-Zadeh A, Mackie EJ, Whitton RC.To gain a better understanding of the aetiology of articular surface collapse in horses with palmar osteochondral disease. Objective: To determine whether acceleration of focal bone resorption associated with reduced physical activity contributes to articular surface collapse in racehorses with palmar osteochondral disease. Methods: Cross-sectional study comparing metacarpal bones from horses at varying stages of race training. Methods: Metacarpal bones from 36 racing Thoroughbred horses were examined with high-resolution peripheral quantitative computed tomography to determine the proportion ...
Navigational ultrasound imaging: A novel imaging tool for aiding interventional therapies of equine musculoskeletal injuries.
Equine veterinary journal    March 12, 2015   Volume 48, Issue 2 195-200 doi: 10.1111/evj.12410
Lustgarten M, Redding WR, Schnabel LV, Prange T, Seiler GS.Navigational ultrasound imaging, also known as fusion imaging, is a novel technology that allows real-time ultrasound imaging to be correlated with a previously acquired computed tomography (CT) or magnetic resonance imaging (MRI) study. It has been used in man to aid interventional therapies and has been shown to be valuable for sampling and assessing lesions diagnosed with MRI or CT that are equivocal on ultrasonography. To date, there are no reports of the use of this modality in veterinary medicine. Objective: To assess whether navigational ultrasound imaging can be used to assist commonly...
Detection of early osteoarthritis in the centrodistal joints of Icelandic horses: Evaluation of radiography and low-field magnetic resonance imaging.
Equine veterinary journal    January 30, 2015   Volume 48, Issue 1 57-64 doi: 10.1111/evj.12370
Ley CJ, Björnsdóttir S, Ekman S, Boyde A, Hansson K.Validated noninvasive detection methods for early osteoarthritis (OA) are required for OA prevention and early intervention treatment strategies. Objective: To evaluate radiography and low-field magnetic resonance imaging (MRI) for the detection of early stage OA osteochondral lesions in equine centrodistal joints using microscopy as the reference standard. Methods: Prospective imaging of live horses and imaging and microscopy of cadaver tarsal joints. Methods: Centrodistal (distal intertarsal) joints of 38 Icelandic research horses aged 27-29 months were radiographed. Horses were subjected to...
Central corneal thickness measurements and ultrasonographic study of the growing equine eye.
Veterinary ophthalmology    January 27, 2015   Volume 18, Issue 6 462-471 doi: 10.1111/vop.12252
Herbig LE, Eule JC.The aim of this study was to determine the central corneal thickness (CCT) of healthy equine eyes with high-frequency ultrasound (HF) and ultrasound biomicroscopy (UBM) and to measure the axial dimensions with B-mode ultrasound. Additionally, the influence of the age on the measured dimensions of the globe was analyzed. Methods: Fifty warm blood horses (mean age 23.0 ± 21.12 months) were divided into 3 age groups (group 1: 0-6 months; group 2: ≥7-30 months; and group 3: ≥31-78 months). Methods: Corneal measurements were performed with a 22-MHz and a 50-MHz transducer. Crystalline lens thi...
Relationship between the shape of the central and third tarsal bones and the presence of tarsal osteoarthritis.
Veterinary journal (London, England : 1997)    January 13, 2015   Volume 204, Issue 1 94-98 doi: 10.1016/j.tvjl.2015.01.006
Sprackman L, Dakin SG, May SA, Weller R.Osteoarthritis (OA) of the low motion joints of the tarsus, commonly termed 'bone spavin', is common in horses. Whilst the aetiology of this condition is multifactorial, it has been suggested that dorsal wedging of the central tarsal bone (CTB) and third tarsal bone (TTB) may predispose to the development of this disease. The aim of this study was to investigate the relationship between tarsal bone conformation and osteoarthritis of the proximal intertarsal (PIT), distal intertarsal (DIT) and tarsometatarsal (TMT) joints. It was hypothesised that wedging of the CTB and TTB would be associated ...
Comparison of a new laser beam wound camera and a digital photoplanimetry-based method for wound measurement in horses.
Veterinary journal (London, England : 1997)    January 5, 2015   Volume 203, Issue 3 309-314 doi: 10.1016/j.tvjl.2014.12.035
Van Hecke LL, De Mil TA, Haspeslagh M, Chiers K, Martens AM.The aim of this study was to compare the accuracy, precision, inter- and intra-operator reliability of a new laser beam (LB) wound camera and a digital photoplanimetry-based (DPB) method for measuring the dimensions of equine wounds. Forty-one wounds were created on equine cadavers. The area, circumference, maximum depth and volume of each wound were measured four times with both techniques by two operators. A silicone cast was made of each wound and served as the reference standard to measure the wound dimensions. The DPB method had a higher accuracy and precision in determining the wound vol...
Comparison of radiographic and computed tomographic images of the lungs in healthy neonatal foals.
American journal of veterinary research    December 24, 2014   Volume 76, Issue 1 42-52 doi: 10.2460/ajvr.76.1.42
Schliewert EC, Lascola KM, O'Brien RT, Clark-Price SC, Wilkins PA, Foreman JH, Mitchell MA, Hartman SK, Kline KH.To compare CT and radiographic images of the lungs in sedated healthy foals positioned in sternal recumbency and to investigate whether a relationship exists between CT-derived measurements of lung attenuation and Paco2 and Pao2. Methods: 6 healthy Standardbred foals < 14 days of age. Methods: Thoracic CT images were acquired followed by radiographic views with each foal sedated and positioned in sternal recumbency. For each foal, both CT and radiographic images were evaluated for severity and extent of changes by lung regions on the basis of a subjective scoring system by 3 investigators. ...
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