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Topic:Veterinary Science
Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Catalase activity in equine semen. To characterize the activity of catalase in equine semen. Methods: 15 stallions of known and unknown reproductive history. Methods: Seminal plasma was collected from raw equine semen by centrifugation, and samples of seminal plasma were frozen prior to assay for catalase activity. Tissue samples (n = 3 stallions) from the bulbourethral gland, prostate gland, vesicular gland, and testis were homogenized, and cauda epididymal fluid was collected for determination of catalase activity. Catalase activity was determined as an enzyme kinetic assay by the disappearance of H2O2 as measured by ultravio...
Genotyping of Bacteroides fragilis isolates from stool specimens by arbitrarily-primed-PCR. In order to determine genetic relatedness of Bacteroides fragilis isolates from different clinical sources, arbitrarily primed polymerase chain reaction (PCR) (AP-PCR) was used to compare 17 strains isolated from patients with inflammatory bowel disease (IBD) and 20 strains isolated from foals with diarrhea. Three reference ATCC strains were also analyzed. Eighteen unique types were identified with a 22-mer arbitrary primer (ERIC-2) among the 20 patient isolates. Types 1 (enterotoxigenic) and 9 (nonenterotoxigenic), were each found in the stools of two patients. All other isolates showed a dis...
The effects of treadmill inclination and speed on the activity of two hindlimb muscles in the trotting horse. Electromyographic activity (EMG) was used to determine how hindlimb muscle activation patterns vary with speed and incline in the horse. EMG was recorded using surface electrodes over the gluteus medius and tensor fasciae latae muscles during treadmill locomotion at trot for different combinations of speed (3.5 to 6 m/s) and inclination (0, 3 and 6%). Raw EMG signals were processed to determine stride duration, activity onset and end, and integrated EMG (IEMG). Stride and stance phase duration decreased linearly with increasing speed. Stride duration was not influenced by the slope. Onset and ...
Effect of water restriction on equine behaviour and physiology. Six pregnant mares were used to determine what level of water restriction causes physiological and/or behavioural changes indicative of stress. Nonlegume hay was fed ad libitum. During the first week of restriction, 5 l water/100 kg bwt was available, during the second week 4 l/100 kg bwt and, during the third week, 3 l/100 kg bwt. Ad libitum water intake was 6.9 l/100 kg bwt; at 3 l/100 kg bwt water intake was 42% of this. Daily hay intake fell significantly with increasing water restriction from 12.9 +/- 0.75 kg to 8.3 +/- 0.54 kg; bodyweight fell significantly for a total loss of 48.5 +/- 8...
Effects of exercise intensity and duration on plasma beta-endorphin concentrations in horses. To determine the relationship between plasma beta-endorphin (EN) concentrations and exercise intensity and duration in horses. Methods: 8 mares with a mean age of 6 years (range, 3 to 13 years) and mean body weight of 450 kg. Methods: Horses were exercised for 20 minutes at 60% of maximal oxygen consumption (VO2max) and to fatigue at 95% V02max. Plasma EN concentrations were determined before exercise, after a 10-minute warmup period, after 5, 10, 15, and 20 minutes at 60% VO2max or at the point of fatigue (95% VO2max), and at regular intervals after exercise. Glucose concentrations were deter...
The forelimb in walking horses: 1. Kinematics and ground reaction forces. Video (60 Hz) and force (2000 Hz) data were collected from 5 sound horses during walking. Forelimb data were analysed for 8 strides (4 left, 4 right) per horse to determine sagittal plane kinematics and ground reaction forces (GRFs). The results suggested that brachial rotation was responsible for protraction and retraction of the limb as a whole, while rotations of the scapula and antebrachium elevated the distal limb during breakover and early swing then lowered it in preparation for ground contact. The coffin joint was flexed maximally at the time of peak longitudinal braking force, which o...
Detection of DNA in ancient bones using histochemical methods. We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient bones. The bones examined were fragments of femurs from two human individuals found in the Pompeian C. I. Polybius house and fragments of metacarpals from two horses (Equus sp.) found in the Pompeian "Casti Amanti" house. Both buildings were buried by the 79 A. D. Vesuvius eruption. Fragments of femurs from a modern horse, a modern swine and a modern amphibian also were studied as controls. Some bone sections were stained with two different DNA-specific fluorochromes, 4'-'6-diamidino-2-phenylindole ...
Detection of biological threat agents by immunomagnetic microsphere-based solid phase fluorogenic- and electro-chemiluminescence. This article reviews the recent development of two solid-phase chemiluminescence-based techniques, fluorogenic-chemiluminescence (FCL) and electro-chemiluminescence (ECL) for detection of biological threat agents. Both techniques entail a labeled sandwich immunoassay. The objectives of this work are to develop advanced techniques for sensitive and effective detection of a target analyte, particularly in cases where the analysis includes complex samples containing multiple contaminating factors. Other important considerations in developing such detection techniques include the ease of use, the ...
Concentrations of cadmium, lead and zinc in livestock feed and organs around a metal production centre in eastern Kazakhstan. This paper presents results of analysis of animal feed and meat (cattle, horse and sheep) products from a metal processing region (Oskemen) in east Kazakhstan. Samples were collected from a range of districts of differing distances from the main source of anthropogenic pollution and with differing underlying metal-containing geologies. Analyses for cadmium, lead and zinc revealed high concentrations in many feed and meat samples. Horse (an important food animal) samples had higher levels of contamination than cattle, which were higher than sheep. For example, mean cadmium concentrations in hor...
A unique exocelom-like space during early pregnancy in the horse. The free allantois and allantochorion of conceptuses from 17 mares between 20 and 90 days of gestation were examined to determine the manner in which the associated mesodermal derivatives differentiated. It was found that a robust basement membrane developed under the allantoic endoderm, and that this basement membrane was partially isolated from the vascular layer of the allantois by a mesothelial layer and an exocelom-like space. The exocelom-like space persisted until approximately the stage of villous formation, and remnants of the space persisted over larger allantoic vessels even later. ...
Light and scanning electron microscopic studies of the nasal turbinates of the horse. The nasal turbinates of 5 young horses were studied by light and scanning electron-microscopy. Stratified cuboidal epithelium lined the rostral part of the dorsal and ventral nasal turbinates of the vestibular region. The polyangular microvillus cells of this region were separated by linear depressions. The mid and caudal parts of the dorsal and ventral nasal turbinates and the rostral part of the ethmoturbinates were lined by pseudostratified columnar ciliated respiratory epithelium. Numerous cilia with dilated blebs on the ciliated cells concealed adjacent non-ciliated supporting cells and g...
Streptococcal toxic shock in a horse. A 14-year-old horse was admitted to the veterinary hospital for treatment of tachycardia and lethargy. Initial diagnoses were ventricular tachycardia and renal dysfunction. During hospitalization other findings included fever, renal failure, hepatic failure, hypotension, and intermittent ventricular arrhythmias. Bacteriologic culture of 2 blood samples collected during febrile crises 7 days apart yielded Streptococcus mitis. These culture results along with other clinical and physical examination findings fulfill the criteria for a diagnosis of streptococcal toxic shock syndrome, previously de...
[The specific blocks of heterochromatin on metaphase chromosomes of horse and Prjewalski horse detected by in situ digestion with restriction endonucleases]. Restriction endonuclease in situ digestion of metaphase chromosomes gives an opportunity to reveal strips with different structure within GC-rich pericentric heterochromatin of the domestic horse and the wild Przewalski horse. Blocks of heterochromatin, which are insensitive to HaeIII and brightly stained with chromomycin A3 after restriction enzyme digestion, are localized on the border with euchromatin in the majority of chromosomes of Equus caballus and E. przewalskii. In contrast to chromosome 5 of E. caballus, acrocentric chromosomes of E. prezewalskii which are homologous to this chromos...
Effects of transport container and ambient storage temperature on motion characteristics of equine spermatozoa. This study was conducted to compare the cooling rates and storage temperatures within equine semen transport containers exposed to different ambient temperatures, and to evaluate the ability of these containers to preserve spermatozoal motility following 24 h of storage under these conditions. In Experiment 1, nonfat dried milk solids, glucose, sucrose, equine semen extender was divided into seven 40-mL aliquots and loaded into seven different semen transport containers: Equitainer I, Equitainer II, Equitainer III, ExpectaFoal, Bio-Flite, Lane STS, and Equine Express. After containers were loa...
Physiological responses of horses to 24 hours of transportation using a commercial van during summer conditions. Fifteen mature horses (mares, n = 6); geldings, n = 9) were used to assess the physiological responses of 24 h of transport in a commercial van under California summer conditions. The study was conducted on four consecutive days, and data were collected on d 1 and d 2 to obtain baseline values and to determine any diurnal variation in the individual measurements. Travel commenced on d 3 at 0800 for 24 h, with a total of 1,622 km traveled. Blood samples were collected at 0800, 1100, 1400, 2000, and 0200 each day. Horses were weighed and rectal temperatures recorded at 0800 each day and at 2000 ...
Prospects for controlling animal parasitic nematodes by predacious micro fungi. Resistance against anthelmintics is widespread, particularly in parasitic nematode populations of small ruminants. Several new techniques or supplements have been developed or are under investigation. Biological control (BC) is one of these new methods. The net-trapping predacious fungus Duddingtonia flagrans produces thick walled resting spores, chlamydospores, which are able to survive passage through the gastrointestinal tract of cattle, horses, sheep and pigs. Under Danish climatic conditions it has been shown that the number of parasite larvae on pasture and the worm burden of the grazing...
Development of analytical methods for the detection of metaraminol in the horse. Aramine (metaraminol bitartrate) has been found in the possession of horse trainers and veterinarians who have been investigated for possible inappropriate drug administration to racing horses. Metaraminol (3-hydroxyphenylisopropanolamine) is a sympathomimetic amine that directly and indirectly affects adrenergic receptors, with alpha effects being predominant. Because it has the potential to affect the performance of a racing horse, its use is prohibited. In the present study, methods for the detection of metaraminol were developed. Metaraminol was found to be extracted with poor recovery ( 9...
Carbohydrate supplementation of horses during endurance exercise: comparison of fructose and glucose. To delay the onset of fatigue, endurance horses are often fed at rest stops during races. The resulting increase in blood insulin may adversely inhibit lipolysis. In humans, ingestion of fructose produces a smaller insulin rise than glucose. This study compared glucose and fructose as carbohydrate supplements for endurance horses. Three Arabian geldings were given 300 g of fructose (F), glucose (G) or 50% glucose: 50% fructose (GF), in 1.5 L water, by stomach tube. In the Resting Test, carbohydrate was administered at rest. Following treatment, blood samples were taken every 30 min for 8 h, an...
Glycosylation in the near-term epitheliochorial placenta of the horse, donkey and camel: a comparative study of interbreeding and non-interbreeding species. Studies from this laboratory have shown great diversity in the glycosylation of tissues comprising the interhaemal barrier of species with different placental types. This diversity may be one of the factors preventing interbreeding between species. Glycan expression within the uterine epithelium and trophoblast of the interhaemal barrier was examined to test this proposition in three species with similar diffuse, microcotyledonary, epitheliochorial allantochorionic types of placenta: the horse (Equus caballus) and donkey (Equus asinus), which can interbreed with each other, and the camel (Came...
Detection of steroidogenic acute regulatory protein in equine ovaries. A steroidogenic acute regulatory (StAR) protein has been identified in several species as a probable important rate-limiting step in steroidogenesis. This protein is believed to be responsible for transporting cholesterol from the outer to the inner mitochondrial membrane. It is known that equine chorionic gonadotrophin (eCG) stimulates steroidogenesis in the corpora lutea of early pregnant mares and that eCG also upregulates StAR mRNA in bovine ovaries. In the present study, ovarian tissue from cyclic and early pregnant mares was immunostained to detect the distribution of the StAR protein. W...
Preferred speed and cost of transport: the effect of incline. Preferred speed is the behavioral tendency of animals to utilize a relatively narrow set of speeds near the middle of a much broader range that they are capable of using within a particular gait. Possible explanations for this behavior include minimizing musculoskeletal stresses and maximizing energetic economy. If preferred speed is determined by energetic economy (cost of transport, C(T)), then shifts in preferred speed should produce shifts in C(T). To test this hypothesis, preferred speeds were measured in trotting horses on the level and on an incline. The preferred trotting speed decreas...
