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Home / Equine Research Bank / Immun Inflamm Dis / A comparison of nanoparticullate CpG immunotherapy with and ...
Immunity, inflammation and disease2017; 6(1); 81-96; doi: 10.1002/iid3.198

A comparison of nanoparticullate CpG immunotherapy with and without allergens in spontaneously equine asthma-affected horses, an animal model.

Abstract: New therapeutic strategies to modulate the immune response of human and equine allergic asthma are still under extensive investigation. Immunomodulating agents stimulating T-regulatory cells offer new treatment options beyond conventional symptomatic treatment or specific immunotherapy for human and equine allergic airway diseases, with the goal of a homoeostatic T-helper cell balance. The aim of this study was to evaluate the effects of a nebulized gelatin nanoparticle-CpG formulation (CpG-GNP) with and without specific allergens for the treatment of spontaneous allergic equine asthma as a model for human asthma. Twenty equine asthma-affected horses were treated either with CpG-GNP alone or CpG-GNP with allergens. Two specific allergens were selected for each horse based on history and an in-vitro test. Each horse received seven administrations of the respective nebulized composition and was examined before treatment, immediately after and 6 weeks after the treatment course. Clinical parameters such as breathing rate, indirect interpleural measurement, arterial blood gases, amount of tracheal mucus and percentage of neutrophils and cytokines in tracheal washes and serum samples were evaluated. Treatment with CpG-GNP alone as well as in combinations with relevant allergens resulted in clinical improvement of nasal discharge, breathing rate, amount of secretion and viscosity, neutrophil percentage and partial oxygen pressure directly after and 6 weeks after treatment. There were no significant differences between the two treatments in clinical parameters or local cytokine profiles in the tracheal wash fluid (IL-10, IFN-g, and IL-17). IL-4 concentrations decreased significantly in both groups. Nonspecific CpG-GNP-based immunotherapy shows potential as a treatment for equine and possibly also human allergic asthma.
© 2017 The Authors. Immunity, Inflammation and Disease Published by John Wiley & Sons Ltd.
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Publication Date: 2017-11-01 PubMed ID: 29094511PubMed Central: PMC5818452DOI: 10.1002/iid3.198Google Scholar: Lookup
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  • Clinical Trial
  • Veterinary
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the effectiveness of nanoparticle-CpG immunotherapy, with and without allergens, in treating equine asthma, a condition seen as a model for human asthma. The results indicated that CpG nanoparticle-based immunotherapy can potentially improve asthma symptoms and may be applied in human treatment strategies.

Research Objectives and Approach:

  • The research aimed to assess the impact of nebulized gelatin nanoparticle-CpG formulation, applied with or without allergies, on equine asthma.
  • Twenty horses with asthma were treated either with the nanoparticle formulation alone or with the addition of allergens, based on their history and in-vitro tests.
  • Each horse was subjected to seven administrations of the treatment and was examined prior to treatment, instantaneously afterwards, and six weeks post-treatment.

Methodology and Measurements:

  • Investigators tracked various clinical indicators such as breathing rate, blood gas levels, the volume of tracheal mucus, the count of neutrophils and cytokines in tracheal washes, and serum samples.

Findings:

  • Both the treatment formats led to improvements in symptoms like nasal discharge, rate of respiration, level of secretion and viscosity, the percentage of neutrophils, and partial oxygen pressure, both immediately and six weeks after treatment.
  • No significant differences were noted between the two treatment formats in terms of clinical outcomes or local cytokine profiles (IL-10, IFN-g, and IL-17) in the tracheal wash fluid.
  • The concentration of IL-4 decreased significantly in both treatment groups.

Implications:

  • The study results suggest that nanoparticle-CpG immunotherapy can potentially be used in the treatment of asthma in horses and possibly also in humans.
  • The treatment appears to be effective both with and without the addition of specific allergens, offering flexibility in treatment plans.

Conclusions:

  • The research demonstrated that non-specific CpG-GNP-based immunotherapy has promise as a potential strategy for managing allergic asthma in both horses and potentially humans.

Cite This Article

APA
Klier J, Geis S, Steuer J, Geh K, Reese S, Fuchs S, Mueller RS, Winter G, Gehlen H. (2017). A comparison of nanoparticullate CpG immunotherapy with and without allergens in spontaneously equine asthma-affected horses, an animal model. Immun Inflamm Dis, 6(1), 81-96. https://doi.org/10.1002/iid3.198

Publication

Immunity, inflammation and disease
ISSN: 2050-4527
NlmUniqueID: 101635460
Country: England
Language: English
Volume: 6
Issue: 1
Pages: 81-96

Researcher Affiliations

Klier, John
  • Centre for Clinical Veterinary Medicine, Equine Clinic, Ludwig-Maximilians-University, Munich, Germany.
Geis, Sabine
  • Centre for Clinical Veterinary Medicine, Equine Clinic, Ludwig-Maximilians-University, Munich, Germany.
  • Department of Veterinary Medicine, Equine Clinic, Surgery and Radiology, Free University of Berlin, Berlin, Germany.
Steuer, Jeanette
  • Centre for Clinical Veterinary Medicine, Equine Clinic, Ludwig-Maximilians-University, Munich, Germany.
  • Department of Veterinary Medicine, Equine Clinic, Surgery and Radiology, Free University of Berlin, Berlin, Germany.
Geh, Katharina
  • Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig-Maximilians-University, Munich, Germany.
Reese, Sven
  • Department of Veterinary Science, Institute of Anatomy, Histology and Embryology, Ludwig-Maximilians-University, Munich, Germany.
Fuchs, Sebastian
  • Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig-Maximilians-University, Munich, Germany.
Mueller, Ralf S
  • Centre for Clinical Veterinary Medicine, Small Animal Medicine Clinic, Ludwig-Maximilians-University, Munich, Germany.
Winter, Gerhard
  • Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig-Maximilians-University, Munich, Germany.
Gehlen, Heidrun
  • Department of Veterinary Medicine, Equine Clinic, Surgery and Radiology, Free University of Berlin, Berlin, Germany.

MeSH Terms

  • Allergens / pharmacology
  • Animals
  • Asthma / immunology
  • Asthma / therapy
  • Asthma / veterinary
  • Cytokines / immunology
  • Desensitization, Immunologic
  • Disease Models, Animal
  • Female
  • Horse Diseases / immunology
  • Horse Diseases / therapy
  • Horses
  • Male
  • Nanoparticles / therapeutic use
  • Oligodeoxyribonucleotides / pharmacology
  • Random Allocation

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Citations

This article has been cited 7 times.
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