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BMC veterinary research2020; 16(1); 44; doi: 10.1186/s12917-020-2262-5

Betulinic acid shows anticancer activity against equine melanoma cells and permeates isolated equine skin in vitro.

Abstract: Equine malignant melanoma (EMM) is a frequently occurring dermoepidermal tumor in grey horses. Currently available therapies are either challenging or inefficient. Betulinic acid (BA), a naturally occurring triterpenoid, is a promising compound for cancer treatment. To evaluate the potential of BA as a topical therapy for EMM, its anticancer effects on primary equine melanoma cells and dermal fibroblasts and its percutaneous permeation through isolated equine skin were assessed in vitro. Results: BA showed antiproliferative and cytotoxic effects on both primary equine melanoma cells and fibroblasts in a time- and dose-dependent manner. The lowest half-maximal inhibitory concentrations were obtained 96 h after the beginning of drug exposure (12.7 μmol/L and 23.6 μmol/L for melanoma cells eRGO1 and MelDuWi, respectively, in cytotoxicity assay). High concentrations of the compound were reached in the required skin layers in vitro. Conclusions: BA is a promising substance for topical EMM treatment. Further clinical studies in horses are necessary to assess safety and antitumoral effects in vivo.
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Publication Date: 2020-02-05 PubMed ID: 32024502PubMed Central: PMC7003431DOI: 10.1186/s12917-020-2262-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article outlines a study on betulinic acid (BA), a naturally found compound, and its potential as a topical treatment for equine malignant melanoma (EMM), a common skin cancer in grey horses. The study examines the antiproliferative and cytotoxic effects of BA on equine melanoma cells and how it permeates through equine skin.

Objective of the Research

  • The main goal of this research was to evaluate the efficacy of betulinic acid (BA) as a topical treatment for equine malignant melanoma (EMM). The researchers aimed to determine the effects of BA on equine melanoma cells and fibroblasts, and its potential skin absorption.

Methodology

  • The researchers tested the impact of BA on primary equine melanoma cells and fibroblasts.
  • They measured the effects in a time- and dose-dependent manner.
  • The study also included an in vitro investigation of BA’s skin permeation capacities.

Results

  • The study revealed that BA exhibited both antiproliferative and cytotoxic effects on the melanoma cells and fibroblasts.
  • The effects were recorded as time- and dose-dependent, with the most significant impact seen 96 hours after initial drug exposure.
  • The required skin layers showed a high concentration of the compound throughout the in vitro evaluation.

Conclusions

  • The findings of this research considered BA to be a promising substance for the topical treatment of EMM.
  • However, further research is required to understand its safety and anti-tumor effects in a live situation, necessitating more clinical studies on horses.

Cite This Article

APA
Weber LA, Meißner J, Delarocque J, Kalbitz J, Feige K, Kietzmann M, Michaelis A, Paschke R, Michael J, Pratscher B, Cavalleri JV. (2020). Betulinic acid shows anticancer activity against equine melanoma cells and permeates isolated equine skin in vitro. BMC Vet Res, 16(1), 44. https://doi.org/10.1186/s12917-020-2262-5

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 16
Issue: 1
Pages: 44
PII: 44

Researcher Affiliations

Weber, Lisa A
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559, Hannover, Germany.
Meißner, Jessica
  • Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559, Hanover, Germany. Jessica.Meissner@tiho-hannover.de.
Delarocque, Julien
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559, Hannover, Germany.
Kalbitz, Jutta
  • Biosolutions Halle GmbH, Weinbergweg 22, 06120, Halle (Saale), Germany.
Feige, Karsten
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559, Hannover, Germany.
Kietzmann, Manfred
  • Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559, Hanover, Germany.
Michaelis, Anne
  • Biozentrum, Martin Luther University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany.
Paschke, Reinhard
  • Biozentrum, Martin Luther University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany.
Michael, Julia
  • Skinomics GmbH, Weinbergweg 23, 06120, Halle (Saale), Germany.
Pratscher, Barbara
  • University Small Animal Clinic, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210, Vienna, Austria.
  • University Equine Clinic, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210, Vienna, Austria.
Cavalleri, Jessika-M V
  • University Equine Clinic, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210, Vienna, Austria.

MeSH Terms

  • Animals
  • Antineoplastic Agents / pharmacokinetics
  • Antineoplastic Agents / pharmacology
  • Cell Line, Tumor
  • Fibroblasts / drug effects
  • Horse Diseases / drug therapy
  • Horses
  • Melanoma / drug therapy
  • Melanoma / veterinary
  • Pentacyclic Triterpenes
  • Skin / drug effects
  • Skin Neoplasms / drug therapy
  • Skin Neoplasms / veterinary
  • Triterpenes / pharmacokinetics
  • Triterpenes / pharmacology
  • Betulinic Acid

Conflict of Interest Statement

Manfred Kietzmann is a member of the editorial board of BMC Veterinary Research.

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