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Veterinary surgery : VS2007; 36(7); 613-622; doi: 10.1111/j.1532-950X.2007.00313.x

Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells.

Abstract: To characterize equine adipose tissue-derived stromal cell (ASC) frequency and growth characteristics and assess of their adipogenic and osteogenic differentiation potential. Methods: In vitro experimental study. Methods: Horses (n=5; aged, 9 months to 5 years). Methods: Cell doubling characteristics of ASCs harvested from supragluteal subcutaneous adipose tissue were evaluated over 10 passages. Primary, second (P2), and fourth (P4) passage ASCs were induced under appropriate conditions to undergo adipogenesis and osteogenesis. Limit dilution assays were performed on each passage to determine the frequency of colony-forming units with a fibroblastic (CFU-F) phenotype and the frequency of ASC differentiation into the adipocyte (CFU-Ad) and osteoblast (CFU-Ob) phenotype. Results: ASC isolates exhibited an average cell-doubling time of 2.1+/-0.9 days during the first 10 cell doublings. Approximately 1 in 2.3+/-0.4 of the total stromal vascular fraction nucleated cells were ASCs, based on the CFU-F assays, and 1 in 3.6+/-1.3 expressed alkaline phosphatase, an osteogenic marker. Primary ASCs differentiated in response to adipogenic (1 in 4.9+/-5.4, CFU-Ad) and osteogenic (1 in <2.44, CFU-Ob) inductive conditions and maintained their differentiation potential during subsequent passages (P2 and P4). Conclusions: The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of equine ASCs show some differences to those documented for ASCs in other mammalian species. Conclusions: Adipose tissue is a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.
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Publication Date: 2007-09-27 PubMed ID: 17894587DOI: 10.1111/j.1532-950X.2007.00313.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines the growth characteristics, frequency, and potential for adipogenic and osteogenic differentiation of stromal cells derived from equine adipose tissue. The results indicate that these cells exhibit unique characteristics compared to similar ASCs in other mammals, and show potential for use in tissue engineering applications in veterinary medicine.

Understanding The Research Paper

This research aimed to characterize equine adipose tissue-derived stromal cells (ASCs) in terms of their frequency, growth properties, and ability to transform into adipocytes (fat cells) and osteoblasts (bone cells). These cell types are considered key for tissue engineering, an important field in regenerative medicine.

  • The researchers extracted ASCs from equine subcutaneous adipose tissue, which is a type of fat located beneath the skin of horses. They examined the cell doubling characteristics of these cells over 10 progression stages within a controlled lab environment.
  • Using various tests and assays, they analysed the frequency at which the extracted cells could convert into colony-forming units with fibroblastic phenotype (CFU-F, cell masses capable of producing connective tissues), adipocytes (CFU-Ad) and osteoblasts (CFU-Ob).
  • The study found that the equine ASCs isolated had an average cell doubling time of about 2.1 days during the initial 10 doublings, indicating a stable growth rate in vitro.
  • The tests showed that approximately half of the total stromal vascular fraction cells were ASCs based on the CFU-F assays, and 1 out of every 3.6 cells expressed alkaline phosphatase, a marker indicating the potential to become osteoblasts.
  • Upon inducement under certain conditions, the primary ASCs differentiated into adipocytes and osteoblasts, thereby demonstrating their adipogenic and osteogenic potentials respectively. They also retained this potential for differentiation even in subsequent passages (P2 and P4).

Implications and Significance

The findings of this research offer valuable insights into the characteristics and potentials of equine ASCs. These cells show some differences in feature as compared to ASCs from other mammals. This discovery has crucial implications, especially for veterinary medicine, as these stem cells can be a potential source for tissue engineering applications.

In other words, equine ASCs can potentially be used in regenerative therapies to replace or repair damaged tissues in horses, thereby advancing veterinary medical treatments. However, further research is required to validate these findings and develop potential therapeutic applications.

Cite This Article

APA
Vidal MA, Kilroy GE, Lopez MJ, Johnson JR, Moore RM, Gimble JM. (2007). Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells. Vet Surg, 36(7), 613-622. https://doi.org/10.1111/j.1532-950X.2007.00313.x

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 36
Issue: 7
Pages: 613-622

Researcher Affiliations

Vidal, Martin A
  • Equine Health Studies Program, Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. mvidal@vetmed.lsu.edu
Kilroy, Gail E
    Lopez, Mandi J
      Johnson, Jill R
        Moore, Rustin M
          Gimble, Jeffrey M

            MeSH Terms

            • Adipocytes / cytology
            • Adipocytes / physiology
            • Adipogenesis / physiology
            • Animals
            • Cell Count / veterinary
            • Cell Culture Techniques / methods
            • Cell Culture Techniques / veterinary
            • Cell Differentiation / physiology
            • Cell Division / physiology
            • Colony-Forming Units Assay / veterinary
            • Horses
            • Kinetics
            • Male
            • Osteoblasts / cytology
            • Osteoblasts / physiology
            • Osteogenesis / physiology
            • Stromal Cells / physiology
            • Tissue Engineering / methods
            • Tissue Engineering / veterinary

            Grant Funding

            • P30 DK072476 / NIDDK NIH HHS

            Citations

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