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Home / Equine Research Bank / Vet Surg / Characterization of equine adipose tissue-derived stromal ce...
Veterinary surgery : VS2007; 36(7); 613-622; doi: 10.1111/j.1532-950X.2007.00313.x

Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells.

Abstract: To characterize equine adipose tissue-derived stromal cell (ASC) frequency and growth characteristics and assess of their adipogenic and osteogenic differentiation potential. Methods: In vitro experimental study. Methods: Horses (n=5; aged, 9 months to 5 years). Methods: Cell doubling characteristics of ASCs harvested from supragluteal subcutaneous adipose tissue were evaluated over 10 passages. Primary, second (P2), and fourth (P4) passage ASCs were induced under appropriate conditions to undergo adipogenesis and osteogenesis. Limit dilution assays were performed on each passage to determine the frequency of colony-forming units with a fibroblastic (CFU-F) phenotype and the frequency of ASC differentiation into the adipocyte (CFU-Ad) and osteoblast (CFU-Ob) phenotype. Results: ASC isolates exhibited an average cell-doubling time of 2.1+/-0.9 days during the first 10 cell doublings. Approximately 1 in 2.3+/-0.4 of the total stromal vascular fraction nucleated cells were ASCs, based on the CFU-F assays, and 1 in 3.6+/-1.3 expressed alkaline phosphatase, an osteogenic marker. Primary ASCs differentiated in response to adipogenic (1 in 4.9+/-5.4, CFU-Ad) and osteogenic (1 in <2.44, CFU-Ob) inductive conditions and maintained their differentiation potential during subsequent passages (P2 and P4). Conclusions: The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of equine ASCs show some differences to those documented for ASCs in other mammalian species. Conclusions: Adipose tissue is a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.
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Publication Date: 2007-09-27 PubMed ID: 17894587DOI: 10.1111/j.1532-950X.2007.00313.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines the growth characteristics, frequency, and potential for adipogenic and osteogenic differentiation of stromal cells derived from equine adipose tissue. The results indicate that these cells exhibit unique characteristics compared to similar ASCs in other mammals, and show potential for use in tissue engineering applications in veterinary medicine.

Understanding The Research Paper

This research aimed to characterize equine adipose tissue-derived stromal cells (ASCs) in terms of their frequency, growth properties, and ability to transform into adipocytes (fat cells) and osteoblasts (bone cells). These cell types are considered key for tissue engineering, an important field in regenerative medicine.

  • The researchers extracted ASCs from equine subcutaneous adipose tissue, which is a type of fat located beneath the skin of horses. They examined the cell doubling characteristics of these cells over 10 progression stages within a controlled lab environment.
  • Using various tests and assays, they analysed the frequency at which the extracted cells could convert into colony-forming units with fibroblastic phenotype (CFU-F, cell masses capable of producing connective tissues), adipocytes (CFU-Ad) and osteoblasts (CFU-Ob).
  • The study found that the equine ASCs isolated had an average cell doubling time of about 2.1 days during the initial 10 doublings, indicating a stable growth rate in vitro.
  • The tests showed that approximately half of the total stromal vascular fraction cells were ASCs based on the CFU-F assays, and 1 out of every 3.6 cells expressed alkaline phosphatase, a marker indicating the potential to become osteoblasts.
  • Upon inducement under certain conditions, the primary ASCs differentiated into adipocytes and osteoblasts, thereby demonstrating their adipogenic and osteogenic potentials respectively. They also retained this potential for differentiation even in subsequent passages (P2 and P4).

Implications and Significance

The findings of this research offer valuable insights into the characteristics and potentials of equine ASCs. These cells show some differences in feature as compared to ASCs from other mammals. This discovery has crucial implications, especially for veterinary medicine, as these stem cells can be a potential source for tissue engineering applications.

In other words, equine ASCs can potentially be used in regenerative therapies to replace or repair damaged tissues in horses, thereby advancing veterinary medical treatments. However, further research is required to validate these findings and develop potential therapeutic applications.

Cite This Article

APA
Vidal MA, Kilroy GE, Lopez MJ, Johnson JR, Moore RM, Gimble JM. (2007). Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells. Vet Surg, 36(7), 613-622. https://doi.org/10.1111/j.1532-950X.2007.00313.x

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 36
Issue: 7
Pages: 613-622

Researcher Affiliations

Vidal, Martin A
  • Equine Health Studies Program, Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. mvidal@vetmed.lsu.edu
Kilroy, Gail E
    Lopez, Mandi J
      Johnson, Jill R
        Moore, Rustin M
          Gimble, Jeffrey M

            MeSH Terms

            • Adipocytes / cytology
            • Adipocytes / physiology
            • Adipogenesis / physiology
            • Animals
            • Cell Count / veterinary
            • Cell Culture Techniques / methods
            • Cell Culture Techniques / veterinary
            • Cell Differentiation / physiology
            • Cell Division / physiology
            • Colony-Forming Units Assay / veterinary
            • Horses
            • Kinetics
            • Male
            • Osteoblasts / cytology
            • Osteoblasts / physiology
            • Osteogenesis / physiology
            • Stromal Cells / physiology
            • Tissue Engineering / methods
            • Tissue Engineering / veterinary

            Grant Funding

            • P30 DK072476 / NIDDK NIH HHS

            Citations

            This article has been cited 66 times.
            1. Petrova V, Vachkova E. Outlook of Adipose-Derived Stem Cells: Challenges to Their Clinical Application in Horses.. Vet Sci 2023 May 12;10(5).
              doi: 10.3390/vetsci10050348pubmed: 37235430google scholar: lookup
            2. Andrietti ALP, Durgam SS, Naumann B, Stewart M. Basal and inducible Osterix expression reflect equine mesenchymal progenitor cell osteogenic capacity.. Front Vet Sci 2023;10:1125893.
              doi: 10.3389/fvets.2023.1125893pubmed: 37035801google scholar: lookup
            3. Rothoerl R, Tomelden J, Alt EU. Safety and Efficacy of Autologous Stem Cell Treatment for Facetogenic Chronic Back Pain.. J Pers Med 2023 Feb 28;13(3).
              doi: 10.3390/jpm13030436pubmed: 36983621google scholar: lookup
            4. Bagge J, Berg LC, Janes J, MacLeod JN. Donor age effects on in vitro chondrogenic and osteogenic differentiation performance of equine bone marrow- and adipose tissue-derived mesenchymal stromal cells.. BMC Vet Res 2022 Nov 3;18(1):388.
              doi: 10.1186/s12917-022-03475-2pubmed: 36329434google scholar: lookup
            5. Trachsel DS, Stage HJ, Rausch S, Trappe S, Söllig K, Sponder G, Merle R, Aschenbach JR, Gehlen H. Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine.. Animals (Basel) 2022 Aug 11;12(16).
              doi: 10.3390/ani12162049pubmed: 36009640google scholar: lookup
            6. Rezaei F, Khasaf S, Ghasemi S, Parham A, Mirshokraei P. How to maintain and transport equine adipose tissue for isolating mesenchymal stem cells?. BMC Vet Res 2022 Jul 21;18(1):284.
              doi: 10.1186/s12917-022-03379-1pubmed: 35864533google scholar: lookup
            7. Abd-El-Aziz A, Riveroll A, Esparza-Gonsalez B, McD￮ L, Cohen AM, Fenech AL, Montelpare WJ. Heat Shock Alters the Proteomic Profile of Equine Mesenchymal Stem Cells.. Int J Mol Sci 2022 Jun 29;23(13).
              doi: 10.3390/ijms23137233pubmed: 35806237google scholar: lookup
            8. Song K, Yang GM, Han J, Gil M, Dayem AA, Kim K, Lim KM, Kang GH, Kim S, Jang SB, Vellingiri B, Cho SG. Modulation of Osteogenic Differentiation of Adipose-Derived Stromal Cells by Co-Treatment with 3, 4'-Dihydroxyflavone, U0126, and N-Acetyl Cysteine.. Int J Stem Cells 2022 Aug 30;15(3):334-345.
              doi: 10.15283/ijsc22044pubmed: 35769058google scholar: lookup
            9. Taylor SD, Serpa PBS, Santos AP, Hart KA, Vaughn SA, Moore GE, Mukhopadhyay A, Page AE. Effects of intravenous administration of peripheral blood-derived mesenchymal stromal cells after infusion of lipopolysaccharide in horses.. J Vet Intern Med 2022 Jul;36(4):1491-1501.
              doi: 10.1111/jvim.16447pubmed: 35698909google scholar: lookup
            10. Veronesi F, Maglio M, Contartese D, Martini L, Muttini A, Fini M. Stromal Vascular Fraction and Amniotic Epithelial Cells: Preclinical and Clinical Relevance in Musculoskeletal Regenerative Medicine.. Stem Cells Int 2021;2021:6632052.
              doi: 10.1155/2021/6632052pubmed: 33688354google scholar: lookup
            11. Jifcovici A, Solano MA, Fitzpatrick N, Findji L, Blunn G, Sanghani-Kerai A. Comparison of Fat Harvested from Flank and Falciform Regions for Stem Cell Therapy in Dogs.. Vet Sci 2021 Jan 25;8(2).
              doi: 10.3390/vetsci8020019pubmed: 33503997google scholar: lookup
            12. Bagge J, MacLeod JN, Berg LC. Cellular Proliferation of Equine Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells Decline With Increasing Donor Age.. Front Vet Sci 2020;7:602403.
              doi: 10.3389/fvets.2020.602403pubmed: 33363241google scholar: lookup
            13. Widowati W, Noverina R, Ayuningtyas W, Kurniawan D, Kusuma HSW, Arumwardana S, Artie DS, Sholihah IA, Handayani RAS, Laksmitawati DR, Rinendyaputri R, Rilianawati R, Faried A. Proliferation, Characterization and Differentiation Potency of Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs) Cultured in Fresh Frozen and non-Fresh Frozen Plasma.. Int J Mol Cell Med 2019 Fall;8(4):283-294.
              doi: 10.22088/IJMCM.BUMS.8.4.283pubmed: 32587838google scholar: lookup
            14. Mocchi M, Dotti S, Bue MD, Villa R, Bari E, Perteghella S, Torre ML, Grolli S. Veterinary Regenerative Medicine for Musculoskeletal Disorders: Can Mesenchymal Stem/Stromal Cells and Their Secretome Be the New Frontier?. Cells 2020 Jun 11;9(6).
              doi: 10.3390/cells9061453pubmed: 32545382google scholar: lookup
            15. Mohiuddin OA, O'Donnell BT, Poche JN, Iftikhar R, Wise RM, Motherwell JM, Campbell B, Savkovic SD, Bunnell BA, Hayes DJ, Gimble JM. Human Adipose-Derived Hydrogel Characterization Based on In Vitro ASC Biocompatibility and Differentiation.. Stem Cells Int 2019;2019:9276398.
              doi: 10.1155/2019/9276398pubmed: 32082388google scholar: lookup
            16. Magri C, Schramme M, Febre M, Cauvin E, Labadie F, Saulnier N, François I, Lechartier A, Aebischer D, Moncelet AS, Maddens S. Comparison of efficacy and safety of single versus repeated intra-articular injection of allogeneic neonatal mesenchymal stem cells for treatment of osteoarthritis of the metacarpophalangeal/metatarsophalangeal joint in horses: A clinical pilot study.. PLoS One 2019;14(8):e0221317.
              doi: 10.1371/journal.pone.0221317pubmed: 31465445google scholar: lookup
            17. Yang Q, Pinto VMR, Duan W, Paxton EE, Dessauer JH, Ryan W, Lopez MJ. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates.. Front Bioeng Biotechnol 2019;7:155.
              doi: 10.3389/fbioe.2019.00155pubmed: 31355191google scholar: lookup
            18. Pinheiro LL, de Lima AR, Branco É. Is Stem Cell Commerce in Small Animal Therapies Scientifically and Morally Justified?. Stem Cell Rev Rep 2019 Aug;15(4):506-518.
              doi: 10.1007/s12015-019-09898-zpubmed: 31140074google scholar: lookup
            19. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance.. Cell Transplant 2018 Jan;27(1):93-116.
              doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
            20. Duan W, Chen C, Haque M, Hayes D, Lopez MJ. Polymer-mineral scaffold augments in vivo equine multipotent stromal cell osteogenesis.. Stem Cell Res Ther 2018 Mar 9;9(1):60.
              doi: 10.1186/s13287-018-0790-8pubmed: 29523214google scholar: lookup
            21. Olivera R, Moro LN, Jordan R, Pallarols N, Guglielminetti A, Luzzani C, Miriuka SG, Vichera G. Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses.. Stem Cells Cloning 2018;11:13-22.
              doi: 10.2147/SCCAA.S151763pubmed: 29497320google scholar: lookup
            22. Schröck C, Eydt C, Geburek F, Kaiser L, Päbst F, Burk J, Pfarrer C, Staszyk C. Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia.. Vet Med Sci 2017 Nov;3(4):239-251.
              doi: 10.1002/vms3.74pubmed: 29152317google scholar: lookup
            23. Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB. In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.. Stem Cell Res Ther 2017 Oct 3;8(1):218.
              doi: 10.1186/s13287-017-0639-6pubmed: 28974260google scholar: lookup
            24. Geburek F, Roggel F, van Schie HTM, Beineke A, Estrada R, Weber K, Hellige M, Rohn K, Jagodzinski M, Welke B, Hurschler C, Conrad S, Skutella T, van de Lest C, van Weeren R, Stadler PM. Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial.. Stem Cell Res Ther 2017 Jun 5;8(1):129.
              doi: 10.1186/s13287-017-0564-8pubmed: 28583184google scholar: lookup
            25. Olivera R, Moro LN, Jordan R, Luzzani C, Miriuka S, Radrizzani M, Donadeu FX, Vichera G. In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors.. PLoS One 2016;11(10):e0164049.
              doi: 10.1371/journal.pone.0164049pubmed: 27732616google scholar: lookup
            26. Maumus M, Roussignol G, Toupet K, Penarier G, Bentz I, Teixeira S, Oustric D, Jung M, Lepage O, Steinberg R, Jorgensen C, Noel D. Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.. Front Immunol 2016;7:392.
              doi: 10.3389/fimmu.2016.00392pubmed: 27729913google scholar: lookup
            27. Williams LB, Co C, Koenig JB, Tse C, Lindsay E, Koch TG. Response to Intravenous Allogeneic Equine Cord Blood-Derived Mesenchymal Stromal Cells Administered from Chilled or Frozen State in Serum and Protein-Free Media.. Front Vet Sci 2016;3:56.
              doi: 10.3389/fvets.2016.00056pubmed: 27500136google scholar: lookup
            28. Duan W, Lopez MJ. Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue.. Stem Cell Rev Rep 2016 Apr;12(2):257-68.
              doi: 10.1007/s12015-015-9634-4pubmed: 26537238google scholar: lookup
            29. Salehi H, Amirpour N, Niapour A, Razavi S. An Overview of Neural Differentiation Potential of Human Adipose Derived Stem Cells.. Stem Cell Rev Rep 2016 Feb;12(1):26-41.
              doi: 10.1007/s12015-015-9631-7pubmed: 26490462google scholar: lookup
            30. Arnhold S, Wenisch S. Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine.. Am J Stem Cells 2015;4(1):1-12.
              pubmed: 25973326
            31. Radtke CL, Nino-Fong R, Rodriguez-Lecompte JC, Esparza Gonzalez BP, Stryhn H, McD￮ LA. Osteogenic potential of sorted equine mesenchymal stem cell subpopulations.. Can J Vet Res 2015 Apr;79(2):101-8.
              pubmed: 25852225
            32. Abouhamzeh B, Salehi M, Hosseini A, Masteri-Farahani AR, Fadai F, Heidari MH, Nourozian M, Soleimani M, Khorashadizadeh M, Mossahebi-Mohammadi M, Mansouri A. DNA Methylation and Histone Acetylation Patterns in Cultured Bovine Adipose Tissue-Derived Stem Cells (BADSCs).. Cell J 2015 Winter;16(4):466-75.
              doi: 10.22074/cellj.2015.492pubmed: 25685737google scholar: lookup
            33. Alipour F, Parham A, Kazemi Mehrjerdi H, Dehghani H. Equine adipose-derived mesenchymal stem cells: phenotype and growth characteristics, gene expression profile and differentiation potentials.. Cell J 2015 Winter;16(4):456-65.
              doi: 10.22074/cellj.2015.491pubmed: 25685736google scholar: lookup
            34. Mohanty N, Gulati BR, Kumar R, Gera S, Kumar S, Kumar P, Yadav PS. Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood.. Cytotechnology 2016 Aug;68(4):795-807.
              doi: 10.1007/s10616-014-9831-zpubmed: 25487085google scholar: lookup
            35. Radtke CL, Nino-Fong R, Esparza Gonzalez BP, McD￮ LA. Application of a novel sorting system for equine mesenchymal stem cells (MSCs).. Can J Vet Res 2014 Oct;78(4):290-6.
              pubmed: 25355998
            36. Liao HT, Chen CT. Osteogenic potential: Comparison between bone marrow and adipose-derived mesenchymal stem cells.. World J Stem Cells 2014 Jul 26;6(3):288-95.
              doi: 10.4252/wjsc.v6.i3.288pubmed: 25126378google scholar: lookup
            37. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential.. Stem Cell Res Ther 2014 Feb 21;5(1):25.
              doi: 10.1186/scrt414pubmed: 24559797google scholar: lookup
            38. Mohanty N, Gulati BR, Kumar R, Gera S, Kumar P, Somasundaram RK, Kumar S. Immunophenotypic characterization and tenogenic differentiation of mesenchymal stromal cells isolated from equine umbilical cord blood.. In Vitro Cell Dev Biol Anim 2014 Jun;50(6):538-48.
              doi: 10.1007/s11626-013-9729-7pubmed: 24414976google scholar: lookup
            39. Guest DJ, Ousey JC, Smith MR. Defining the expression of marker genes in equine mesenchymal stromal cells.. Stem Cells Cloning 2008;1:1-9.
              doi: 10.2147/sccaa.s3824pubmed: 24198500google scholar: lookup
            40. Smith RK, Werling NJ, Dakin SG, Alam R, Goodship AE, Dudhia J. Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.. PLoS One 2013;8(9):e75697.
              doi: 10.1371/journal.pone.0075697pubmed: 24086616google scholar: lookup
            41. Xie L, Zhang N, Marsano A, Vunjak-Novakovic G, Zhang Y, Lopez MJ. In vitro mesenchymal trilineage differentiation and extracellular matrix production by adipose and bone marrow derived adult equine multipotent stromal cells on a collagen scaffold.. Stem Cell Rev Rep 2013 Dec;9(6):858-72.
              doi: 10.1007/s12015-013-9456-1pubmed: 23892935google scholar: lookup
            42. Carvalho Ade M, Badial PR, Álvarez LE, Yamada AL, Borges AS, Deffune E, Hussni CA, Garcia Alves AL. Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial.. Stem Cell Res Ther 2013 Jul 22;4(4):85.
              doi: 10.1186/scrt236pubmed: 23876512google scholar: lookup
            43. Tetta C, Consiglio AL, Bruno S, Tetta E, Gatti E, Dobreva M, Cremonesi F, Camussi G. The role of microvesicles derived from mesenchymal stem cells in tissue regeneration; a dream for tendon repair?. Muscles Ligaments Tendons J 2012 Jul;2(3):212-21.
              pubmed: 23738299
            44. Via AG, Frizziero A, Oliva F. Biological properties of mesenchymal Stem Cells from different sources.. Muscles Ligaments Tendons J 2012 Jul;2(3):154-62.
              pubmed: 23738292
            45. Raabe O, Shell K, Goessl A, Crispens C, Delhasse Y, Eva A, Scheiner-Bobis G, Wenisch S, Arnhold S. Effect of extracorporeal shock wave on proliferation and differentiation of equine adipose tissue-derived mesenchymal stem cells in vitro.. Am J Stem Cells 2013;2(1):62-73.
              pubmed: 23671817
            46. Carrade DD, Lame MW, Kent MS, Clark KC, Walker NJ, Borjesson DL. Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().. Cell Med 2012;4(1):1-11.
              doi: 10.3727/215517912X647217pubmed: 23152950google scholar: lookup
            47. Coli A, Nocchi F, Lamanna R, Iorio M, Lapi S, Urciuoli P, Scatena F, Giannessi E, Stornelli MR, Passeri S. Isolation and characterization of equine amnion mesenchymal stem cells.. Cell Biol Int Rep (2010) 2011 Sep 13;18(1):e00011.
              doi: 10.1042/CBR20110004pubmed: 23124164google scholar: lookup
            48. Requicha JF, Viegas CA, Albuquerque CM, Azevedo JM, Reis RL, Gomes ME. Effect of anatomical origin and cell passage number on the stemness and osteogenic differentiation potential of canine adipose-derived stem cells.. Stem Cell Rev Rep 2012 Dec;8(4):1211-22.
              doi: 10.1007/s12015-012-9397-0pubmed: 22773405google scholar: lookup
            49. Mathieu PS, Loboa EG. Cytoskeletal and focal adhesion influences on mesenchymal stem cell shape, mechanical properties, and differentiation down osteogenic, adipogenic, and chondrogenic pathways.. Tissue Eng Part B Rev 2012 Dec;18(6):436-44.
              doi: 10.1089/ten.TEB.2012.0014pubmed: 22741572google scholar: lookup
            50. Baer PC, Geiger H. Adipose-derived mesenchymal stromal/stem cells: tissue localization, characterization, and heterogeneity.. Stem Cells Int 2012;2012:812693.
              doi: 10.1155/2012/812693pubmed: 22577397google scholar: lookup
            51. Reich CM, Raabe O, Wenisch S, Bridger PS, Kramer M, Arnhold S. Isolation, culture and chondrogenic differentiation of canine adipose tissue- and bone marrow-derived mesenchymal stem cells--a comparative study.. Vet Res Commun 2012 Jun;36(2):139-48.
              doi: 10.1007/s11259-012-9523-0pubmed: 22392598google scholar: lookup
            52. Iacono E, Cunto M, Zambelli D, Ricci F, Tazzari PL, Merlo B. Could fetal fluid and membranes be an alternative source for mesenchymal stem cells (MSCs) in the feline species? A preliminary study.. Vet Res Commun 2012 Jun;36(2):107-18.
              doi: 10.1007/s11259-012-9520-3pubmed: 22327440google scholar: lookup
            53. Hsiao ST, Asgari A, Lokmic Z, Sinclair R, Dusting GJ, Lim SY, Dilley RJ. Comparative analysis of paracrine factor expression in human adult mesenchymal stem cells derived from bone marrow, adipose, and dermal tissue.. Stem Cells Dev 2012 Aug 10;21(12):2189-203.
              doi: 10.1089/scd.2011.0674pubmed: 22188562google scholar: lookup
            54. Violini S, Gorni C, Pisani LF, Ramelli P, Caniatti M, Mariani P. Isolation and differentiation potential of an equine amnion-derived stromal cell line.. Cytotechnology 2012 Jan;64(1):1-7.
              doi: 10.1007/s10616-011-9398-xpubmed: 21994048google scholar: lookup
            55. Mensing N, Gasse H, Hambruch N, Haeger JD, Pfarrer C, Staszyk C. Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.. BMC Vet Res 2011 Aug 2;7:42.
              doi: 10.1186/1746-6148-7-42pubmed: 21810270google scholar: lookup
            56. Raabe O, Shell K, Würtz A, Reich CM, Wenisch S, Arnhold S. Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells.. Vet Res Commun 2011 Aug;35(6):355-65.
              doi: 10.1007/s11259-011-9480-zpubmed: 21614641google scholar: lookup
            57. Fortier LA, Travis AJ. Stem cells in veterinary medicine.. Stem Cell Res Ther 2011 Feb 23;2(1):9.
              doi: 10.1186/scrt50pubmed: 21371354google scholar: lookup
            58. Bodle JC, Hanson AD, Loboa EG. Adipose-derived stem cells in functional bone tissue engineering: lessons from bone mechanobiology.. Tissue Eng Part B Rev 2011 Jun;17(3):195-211.
              doi: 10.1089/ten.TEB.2010.0738pubmed: 21338267google scholar: lookup
            59. Spencer ND, Chun R, Vidal MA, Gimble JM, Lopez MJ. In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells.. Vet J 2012 Feb;191(2):231-9.
              doi: 10.1016/j.tvjl.2010.12.030pubmed: 21315625google scholar: lookup
            60. Fite BZ, Decaris M, Sun Y, Sun Y, Lam A, Ho CK, Leach JK, Marcu L. Noninvasive multimodal evaluation of bioengineered cartilage constructs combining time-resolved fluorescence and ultrasound imaging.. Tissue Eng Part C Methods 2011 Apr;17(4):495-504.
              doi: 10.1089/ten.tec.2010.0368pubmed: 21303258google scholar: lookup
            61. Raabe O, Reich C, Wenisch S, Hild A, Burg-Roderfeld M, Siebert HC, Arnhold S. Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells.. Histochem Cell Biol 2010 Dec;134(6):545-54.
              doi: 10.1007/s00418-010-0760-4pubmed: 21076963google scholar: lookup
            62. Ahmadian Kia N, Bahrami AR, Ebrahimi M, Matin MM, Neshati Z, Almohaddesin MR, Aghdami N, Bidkhori HR. Comparative analysis of chemokine receptor's expression in mesenchymal stem cells derived from human bone marrow and adipose tissue.. J Mol Neurosci 2011 Jul;44(3):178-85.
              doi: 10.1007/s12031-010-9446-6pubmed: 20938756google scholar: lookup
            63. Colleoni S, Bottani E, Tessaro I, Mari G, Merlo B, Romagnoli N, Spadari A, Galli C, Lazzari G. Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor.. Vet Res Commun 2009 Dec;33(8):811-21.
              doi: 10.1007/s11259-009-9229-0pubmed: 19472068google scholar: lookup
            64. Koch TG, Berg LC, Betts DH. Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine.. Can Vet J 2009 Feb;50(2):155-65.
              pubmed: 19412395
            65. Vidal MA, Robinson SO, Lopez MJ, Paulsen DB, Borkhsenious O, Johnson JR, Moore RM, Gimble JM. Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow.. Vet Surg 2008 Dec;37(8):713-24.
              doi: 10.1111/j.1532-950X.2008.00462.xpubmed: 19121166google scholar: lookup
            66. da Silva Meirelles L, Sand TT, Harman RJ, Lennon DP, Caplan AI. MSC frequency correlates with blood vessel density in equine adipose tissue.. Tissue Eng Part A 2009 Feb;15(2):221-9.
              doi: 10.1089/ten.tea.2008.0103pubmed: 18847356google scholar: lookup
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