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BMC veterinary research2011; 7; 48; doi: 10.1186/1746-6148-7-48

Comparison of genomic and proteomic data in recurrent airway obstruction affected horses using Ingenuity Pathway Analysis®.

Abstract: Recurrent airway obstruction (RAO) is a severe chronic respiratory disease affecting horses worldwide, though mostly in the Northern hemisphere. Environmental as well as genetic factors strongly influence the course and prognosis of the disease. Research has been focused on characterization of immunologic factors contributing to inflammatory responses, on genetic linkage analysis, and, more recently, on proteomic analysis of airway secretions from affected horses. The goal of this study was to investigate the interactions between eight candidate genes previously identified in a genetic linkage study and proteins expressed in bronchoalveolar lavage fluid (BALF) collected from healthy and RAO-affected horses. The analysis was carried out with Ingenuity Pathway Analysis® bioinformatics software. Results: The gene with the greatest number of indirect interactions with the set of proteins identified is Interleukin 4 Receptor (IL-4R), whose protein has also been detected in BALF. Interleukin 21 receptor and chemokine (C-C motif) ligand 24 also showed a large number of interactions with the group of detected proteins. Protein products of other genes like that of SOCS5, revealed direct interactions with the IL-4R protein. The interacting proteins NOD2, RPS6KA5 and FOXP3 found in several pathways are reported regulators of the NFκB pathway. Conclusions: The pathways generated with IL-4R highlight possible important intracellular signaling cascades implicating, for instance, NFκB. Furthermore, the proposed interaction between SOCS5 and IL-4R could explain how different genes can lead to identical clinical RAO phenotypes, as observed in two Swiss Warmblood half sibling families because these proteins interact upstream of an important cascade where they may act as a functional unit.
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Publication Date: 2011-08-15 PubMed ID: 21843342PubMed Central: PMC3174119DOI: 10.1186/1746-6148-7-48Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study delves into the genetic and proteomic factors that interact in horses affected by Recurrent Airway Obstruction (RAO), a chronic respiratory disease. Using bioinformatics software, the researchers examined links between genetic markers and proteins found in the respiratory fluid of both healthy and RAO-affected horses.

Objective and Methodology

  • The main objective of this study was to understand the interplay between previously identified genetic markers, or candidate genes, and the proteins identified in bronchoalavolar lavage fluid (BALF) – a fluid collected from the lungs. The researchers studied this in both healthy horses and those affected by Recurrent Airway Obstruction (RAO).
  • Using a bioinformatics software called Ingenuity Pathway Analysis®, the researchers were able to track and analyze the complex interactions between these genes and proteins.

Findings

  • The key outcome of this study is that the Interleukin 4 Receptor (IL-4R) gene had the highest number of indirect interactions with the identified group of proteins. The protein product of this gene was also detected in the BALF of the horses.
  • Two other genes, the Interleukin 21 receptor and chemokine (C-C motif) ligand 24, also showed a significant number of interactions with the studied protein group.
  • The protein product of SOCS5, another gene, exhibited direct interactions with the IL-4R protein.
  • Three proteins (NOD2, RPS6KA5, and FOXP3) that interacted in multiple pathways were found to be regulators of the NFκB pathway, a critical cellular signaling pathway.

Conclusion

  • The identified interactions centered around the IL-4R gene and its protein product could indicate the importance of certain cellular signaling pathways, such as that of NFκB, in the pathogenesis of RAO.
  • In addition, the study explores an interaction between proteins SOCS5 and IL-4R, suggesting how variations in different genes could result in the same RAO clinical symptoms. This is exemplified by the two Swiss Warmblood half-sibling families that share similar RAO phenotypes, and the proteins potentially act together in an upstream step of a pivotal cascade forming a functional unit.

Cite This Article

APA
Racine J, Gerber V, Feutz MM, Riley CP, Adamec J, Swinburne JE, Couetil LL. (2011). Comparison of genomic and proteomic data in recurrent airway obstruction affected horses using Ingenuity Pathway Analysis®. BMC Vet Res, 7, 48. https://doi.org/10.1186/1746-6148-7-48

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 7
Pages: 48

Researcher Affiliations

Racine, Julien
  • Department of Clinical Veterinary Medicine, Vetsuisse-Faculty, University of Berne, Berne, Switzerland.
Gerber, Vinzenz
    Feutz, Marybeth Miskovic
      Riley, C Paige
        Adamec, Jiri
          Swinburne, June E
            Couetil, Laurent L

              MeSH Terms

              • Airway Obstruction / genetics
              • Airway Obstruction / immunology
              • Airway Obstruction / veterinary
              • Animals
              • Bronchoalveolar Lavage Fluid / immunology
              • Computational Biology / methods
              • Gene Expression Regulation / immunology
              • Genomics / methods
              • Horse Diseases / genetics
              • Horse Diseases / immunology
              • Horses
              • Proteomics / methods
              • Receptors, Interleukin-4 / genetics
              • Receptors, Interleukin-4 / immunology
              • Suppressor of Cytokine Signaling Proteins / genetics
              • Suppressor of Cytokine Signaling Proteins / immunology

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