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Home / Equine Research Bank / PLoS One / Comparison of the microbiome, metabolome, and lipidome of ob...
PloS one2019; 14(4); e0215918; doi: 10.1371/journal.pone.0215918

Comparison of the microbiome, metabolome, and lipidome of obese and non-obese horses.

Abstract: Metabolic diseases such as obesity and type 2 diabetes in humans have been linked to alterations in the gastrointestinal microbiota and metabolome. Knowledge of these associations has improved our understanding of the pathophysiology of these diseases and guided development of diagnostic biomarkers and therapeutic interventions. The cellular and molecular pathophysiology of equine metabolic syndrome (EMS) and obesity in horses, however, remain ill-defined. Thus, the objectives of this study were to characterize the fecal microbiome, fecal metabolome, and circulating lipidome in obese and non-obese horses. The fecal microbiota, fecal metabolome, and serum lipidome were evaluated in obese (case) horses (n = 20) and non-obese (control) horses (n = 20) matched by farm of origin (n = 7). Significant differences in metabolites of the mitochondrial tricarboxylic acid cycle and circulating free fatty acids were identified in the obese horses compared to the non-obese horses. These results indicate that the host and bacterial metabolism should be considered important in obese horses. Further studies to determine whether these associations are causal and the mechanistic basis of the association are warranted because they might reveal diagnostic biomarkers and therapeutic interventions to mitigate obesity, EMS, and sequelae including laminitis.
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Publication Date: 2019-04-23 PubMed ID: 31013335PubMed Central: PMC6478336DOI: 10.1371/journal.pone.0215918Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the link between the gastrointestinal microbiome and metabolome and obesity, specifically in horses. It finds significant differences in certain metabolites and circulating free fatty acids in obese horses, suggesting that host and bacterial metabolism play a role in equine obesity.

Research Objective

  • The aim of the research was to explore the pathophysiology of equine metabolic syndrome (EMS) and obesity in horses, which is currently not well understood. Specifically, the study sought to characterize the fecal microbiome, fecal metabolome, and circulating lipidome in obese and non-obese horses.

Methodology

  • For this study, a total of 20 obese horses and 20 non-obese horses from the same farm were selected. These two groups of horses were carefully matched by their origins to control for potential external influencing factors.
  • The scientists analyzed the fecal microbiota, fecal metabolome, and serum lipidome of both groups of horses to identify potential differences related to obesity.

Key Findings

  • Significant differences were observed in the metabolites related to the mitochondrial tricarboxylic acid cycle and circulating free fatty acids in obese horses compared to non-obese horses. This observation indicates alterations in both host and bacterial metabolism in obese horses, highlighting the role of these aspects in equine obesity.
  • These findings suggest that the horse’s own metabolism, as well as the metabolism of its gut microbiota, may play an important role in obesity and metabolic diseases in horses.

Future Research Implications

  • Despite these findings, it is yet to be determined if the identified associations are causal or coincidental, warranting further studies to clarify these aspects. Understanding these connections more thoroughly may uncover new diagnostic biomarkers and therapeutic interventions for equine metabolic syndrome and obesity.
  • The research could potentially lead to breakthroughs in preventing and treating associated conditions, such as laminitis, a painful and debilitating disease affecting the horse’s hooves, that often follows as a consequence of EMS and obesity.

Cite This Article

APA
Coleman MC, Whitfield-Cargile CM, Madrigal RG, Cohen ND. (2019). Comparison of the microbiome, metabolome, and lipidome of obese and non-obese horses. PLoS One, 14(4), e0215918. https://doi.org/10.1371/journal.pone.0215918

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 14
Issue: 4
Pages: e0215918
PII: e0215918

Researcher Affiliations

Coleman, Michelle C
  • Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, United States of America.
Whitfield-Cargile, Canaan M
  • Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, United States of America.
Madrigal, Rodolfo G
  • Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, United States of America.
Cohen, Noah D
  • Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, United States of America.

MeSH Terms

  • Animals
  • Gastrointestinal Microbiome / genetics
  • Horses / genetics
  • Horses / metabolism
  • Horses / microbiology
  • Humans
  • Lipidomics
  • Lipids / blood
  • Metabolic Syndrome / genetics
  • Metabolic Syndrome / microbiology
  • Metabolome / genetics
  • Microbiota / genetics
  • Obesity / genetics
  • Obesity / metabolism
  • Obesity / veterinary

Conflict of Interest Statement

The authors have declared that no competing interests exist.

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Citations

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