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Home / Equine Research Bank / Front Vet Sci / Donor-Matched Comparison of Chondrogenic Potential of Equine...
Frontiers in veterinary science2017; 3; 121; doi: 10.3389/fvets.2016.00121

Donor-Matched Comparison of Chondrogenic Potential of Equine Bone Marrow- and Synovial Fluid-Derived Mesenchymal Stem Cells: Implications for Cartilage Tissue Regeneration.

Abstract: Mesenchymal stem cells (MSCs) have been demonstrated to be useful for cartilage tissue regeneration. Bone marrow (BM) and synovial fluid (SF) are promising sources for MSCs to be used in cartilage regeneration. In order to improve the clinical outcomes, it is recommended that prior to clinical use, the cellular properties and, specifically, their chondrogenic potential must be investigated. The purpose of this study is to compare and better understand the chondrogenic potential of equine bone marrow-derived mesenchymal stem cells (BMMSCs) and synovial fluid-derived mesenchymal stem cells (SFMSCs) populated from the same equine donor. BM- and SF-derived MSCs cultures were generated from five equine donors, and the MSCs were evaluated for their morphology, proliferation, trilineage differentiation, and immunophenotyping. Differences in their chondrogenic potentials were further evaluated quantitatively using glycosaminoglycan (GAG) content and immunofluorescence of chondrogenic differentiation protein markers, SRY-type HMG box9, Aggrecan, and collagen II. The BMMSCs and SFMSCs were similar in cellular morphology, viability, and immunophenotype, but, varied in their chondrogenic potential, and expression of the key chondrogenic proteins. The SFMSCs exhibited a significant increase in GAG content compared to the BMMSCs ( < 0.0001) in three donors, suggesting increased levels of chondrogenesis. The expression of the key chondrogenic proteins correlated positively with the GAG content, suggesting that the differentiation process is dependent on the expression of the target proteins in these three donors. Our findings suggest that even though SFMSCs were hypothesized to be more chondrogenic relative to BMMSCs, there was considerable donor-to-donor variation in the primary cultures of MSCs which can significantly affect their downstream application.
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Publication Date: 2017-01-18 PubMed ID: 28149840PubMed Central: PMC5241318DOI: 10.3389/fvets.2016.00121Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the comparative potential of bone marrow-derived and synovial fluid-derived mesenchymal stem cells for cartilage tissue regeneration in horses. The findings illustrate a significant difference in their chondrogenic potential, which relies heavily on the expression of target proteins.

Introduction and Methodology

  • The study aims to explore the efficiency of mesenchymal stem cells (MSCs) derived from both bone marrow (BM) and synovial fluid (SF) in the process of cartilage regeneration. To assure the clinical effectiveness of these stem cells, it is vital to first investigate their cellular properties and, particularly, their chondrogenic potential, which consists of their ability to alter into cartilage cells.
  • The MSC cultures observed in this study were generated from five horse donors. The methodology involved evaluating the MSCs in terms of their morphology, proliferation, trilineage differentiation, and immunophenotyping, which is a process used to study the protein expression of cells.

Observations and Findings

  • Researchers found that Bone Marrow-Derived Mesenchymal Stem Cells (BMMSCs) and Synovial Fluid-Derived Mesenchymal Stem Cells (SFMSCs) shared similarities in cellular morphology, viability, and immunophenotype, but significantly varied in their chondrogenic potential and in the expression of certain key proteins that lead to cartilage differentiation.
  • SFMSCs showed a significant increase in glycosaminoglycan (GAG) content, a major component of the extracellular matrix in cartilage tissue, in three out of five donors. This suggests an increased level of chondrogenesis or cartilage formation.
  • Importantly, the expression of SRY-type HMG box9, Aggrecan, and Collagen II proteins, which are crucial to chondrogenic differentiation, were found to line up positively with the GAG content, thereby implying that the differentiation process is dependent on the expression of these proteins.

Conclusion and Future Considerations

  • The study concludes that even though it was hypothesized that SFMSCs might possess a higher chondrogenic potential relative to BMMSCs, there is a great amount of variation from donor to donor in the primary cultures of MSCs which could significantly impact their eventual usage.
  • This indicates a strong requirement for the further exploration of individual donor factors that could influence the efficiency of SFMSCs and BMMSCs for cartilage regeneration.

Cite This Article

APA
Zayed M, Caniglia C, Misk N, Dhar MS. (2017). Donor-Matched Comparison of Chondrogenic Potential of Equine Bone Marrow- and Synovial Fluid-Derived Mesenchymal Stem Cells: Implications for Cartilage Tissue Regeneration. Front Vet Sci, 3, 121. https://doi.org/10.3389/fvets.2016.00121

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 3
Pages: 121
PII: 121

Researcher Affiliations

Zayed, Mohammed
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA; Department of Animal Surgery, College of Veterinary Medicine, South Valley University, Qena, Egypt.
Caniglia, Christopher
  • Manor Equine Hospital , Monkton, MD , USA.
Misk, Nabil
  • Department of Animal Surgery, College of Veterinary Medicine, Assiut University , Asyut , Egypt.
Dhar, Madhu S
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee , Knoxville, TN , USA.

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